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The map label for this gene is rnhB

Identifier: 169630303

GI number: 169630303

Start: 3259131

End: 3259835

Strand: Reverse

Name: rnhB

Synonym: MAB_3222c

Alternate gene names: 169630303

Gene position: 3259835-3259131 (Counterclockwise)

Preceding gene: 169630304

Following gene: 169630302

Centisome position: 64.33

GC content: 63.55

Gene sequence:

>705_bases
ATGAGTACCAGCTGGCCGCCACGCACGGTGATTCGCAAGGCATCCGGATTGCGAACGCTCGAATTGACGCTCGACAGAGT
GGGTTTAGGCCCGGTCGCCGGTGTGGACGAGGCGGGACGTGGGGCCTGCGCGGGTCCGTTGGTGATCGCGGCCTGTGTGC
TGGGCTCCAATCAGCGTAAGAGCCTCGCCGCGCTTAACGACTCGAAGAAGCTCACCGAGAAGATGCGCGAGACGCTGTTT
CCTCTCATCTGCCGGTACGCCGAGGCCTACCATGTGGTGTCGGTGCCTGCCGATGAGGTGGACCGCATTGGCGTTCACGT
GGCCAATATCGAGGGAATGCGCCGGGCGGTGGCAGGTCTGGGCGTAAAGCCCGGGTATGTGCTGACGGACGGTTTCCGGG
TGCCGGGATTGCCGGTGCCGTCGCTGCCGGTGATCGGGGGTGACGCGTCGGCGGCCTGTATCGCGGCGGCCAGCGTATTG
GCGAAGGTGAGCCGGGACCGGGTGATGGTGAACATGGATTCCGATCACCCTGGATACGGATTCGCGGTACACAAGGGGTA
CAGCACAGCCGTCCACACCGAGGCATTGCGGCGTCTGGGACCATCGGCTCAGCACCGACAGTCGTTCGTGAATGTCCGTA
ACGCGGCCATGGGAAGTTCTTTGATGCGGCCGCTTGCGCCAAGAGAATCGGATACAGGGGGATAA

Upstream 100 bases:

>100_bases
CCCGTGGCCAACGTCATCGGCAAGGCGCGATTCATCGCATGGCCGCCCGGTCGTTGGGGTGCGGTCAAGACGGTTAATCC
CCAGACCTAGCTTTCGCGGG

Downstream 100 bases:

>100_bases
TGGAAGGCCTATGAGCGCCGAAGACCTCGAAAAGTACGAAACCGAGATGGAACTCTCGCTATATCGCGAGTACAAGGACA
TCGTTGGGCAGTTCAGCTAT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSTSWPPRTVIRKASGLRTLELTLDRVGLGPVAGVDEAGRGACAGPLVIAACVLGSNQRKSLAALNDSKKLTEKMRETLF
PLICRYAEAYHVVSVPADEVDRIGVHVANIEGMRRAVAGLGVKPGYVLTDGFRVPGLPVPSLPVIGGDASAACIAAASVL
AKVSRDRVMVNMDSDHPGYGFAVHKGYSTAVHTEALRRLGPSAQHRQSFVNVRNAAMGSSLMRPLAPRESDTGG

Sequences:

>Translated_234_residues
MSTSWPPRTVIRKASGLRTLELTLDRVGLGPVAGVDEAGRGACAGPLVIAACVLGSNQRKSLAALNDSKKLTEKMRETLF
PLICRYAEAYHVVSVPADEVDRIGVHVANIEGMRRAVAGLGVKPGYVLTDGFRVPGLPVPSLPVIGGDASAACIAAASVL
AKVSRDRVMVNMDSDHPGYGFAVHKGYSTAVHTEALRRLGPSAQHRQSFVNVRNAAMGSSLMRPLAPRESDTGG
>Mature_233_residues
STSWPPRTVIRKASGLRTLELTLDRVGLGPVAGVDEAGRGACAGPLVIAACVLGSNQRKSLAALNDSKKLTEKMRETLFP
LICRYAEAYHVVSVPADEVDRIGVHVANIEGMRRAVAGLGVKPGYVLTDGFRVPGLPVPSLPVIGGDASAACIAAASVLA
KVSRDRVMVNMDSDHPGYGFAVHKGYSTAVHTEALRRLGPSAQHRQSFVNVRNAAMGSSLMRPLAPRESDTGG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=184, Percent_Identity=46.195652173913, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_MYCA9 (B1MDH9)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703952.1
- ProteinModelPortal:   B1MDH9
- SMR:   B1MDH9
- EnsemblBacteria:   EBMYCT00000004298
- GeneID:   5965725
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3222c
- GeneTree:   EBGT00050000016477
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   MABS561007:MAB_3222C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 24621; Mature: 24489

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSWPPRTVIRKASGLRTLELTLDRVGLGPVAGVDEAGRGACAGPLVIAACVLGSNQRK
CCCCCCHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCH
SLAALNDSKKLTEKMRETLFPLICRYAEAYHVVSVPADEVDRIGVHVANIEGMRRAVAGL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHCEEEECHHHHHHHHHHC
GVKPGYVLTDGFRVPGLPVPSLPVIGGDASAACIAAASVLAKVSRDRVMVNMDSDHPGYG
CCCCCEEEECCEECCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
FAVHKGYSTAVHTEALRRLGPSAQHRQSFVNVRNAAMGSSLMRPLAPRESDTGG
EEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
STSWPPRTVIRKASGLRTLELTLDRVGLGPVAGVDEAGRGACAGPLVIAACVLGSNQRK
CCCCCHHHHHHHHCCCEEEEEEHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCH
SLAALNDSKKLTEKMRETLFPLICRYAEAYHVVSVPADEVDRIGVHVANIEGMRRAVAGL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHCEEEECHHHHHHHHHHC
GVKPGYVLTDGFRVPGLPVPSLPVIGGDASAACIAAASVLAKVSRDRVMVNMDSDHPGYG
CCCCCEEEECCEECCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
FAVHKGYSTAVHTEALRRLGPSAQHRQSFVNVRNAAMGSSLMRPLAPRESDTGG
EEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA