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The map label for this gene is mrdA [H]

Identifier: 169630249

GI number: 169630249

Start: 3213327

End: 3215144

Strand: Reverse

Name: mrdA [H]

Synonym: MAB_3167c

Alternate gene names: 169630249

Gene position: 3215144-3213327 (Counterclockwise)

Preceding gene: 169630250

Following gene: 169630247

Centisome position: 63.45

GC content: 64.36

Gene sequence:

>1818_bases
ATGTTCAGACGTGGATGGGTCCTGGCGATGGTCGGCACCGTGCTGCTGGTGGCCGGTGCTGTCGCGTGTACCCCGCGCCC
CGATGGGCCGGGTCCGGTGGCGGAGAAGTTTTTCGAGGCACTGGCCAAGGGCGATACTGCCGCGGCCGCTAAGCTAACCG
ACGACCCCGATGGCGCGAAAGTGGGCCTGGACCAAGCGTTCTCGGGACTACAGGCGACGTCGTTCAAGGCCGCGGTGAAT
GGCTCGCAATACACCCAGGACACCGGTAGTGCGGATGCGACCTATACCTGGCAGCTGCCCAGAAAGCGTGTCTGGACGTA
TAACGGGCGTCTGGAGATGTTGCGTACGGCAGGTAGCTGGCAGGTACGTTGGGCTCCGAGTGACCTGCATCCCAAACTTG
GTGAGCGCCAAATGCTCTCACTGCGCACAGACCCCGCTAAGCGGGCGACGGTCAACGAAGCGGGCGGTACGACGGTGCTG
GCCCCGGCCAATCTGTACAGAATCGCCTTCGATGCCTCGAAGGCCGGCAAATCGTTGATGAGCACCGCCACCGCACTTGC
CGACGCCATTCGGCCCTACGACGACACCATGAACGCCGCATCCCTGGCAGAGCAGGCGAGTGCGCAAACCTCGCCGATGG
ACCTGATCACGCTGCGTAAGGATGACTGGGACAAAGTATCCATCGCGCTGGAGACGCGGCCGGGGGCGCTGCGGCCCGGC
GTGGTGATGACGCCCATCGCCGATCTGTTGCCCACGGACGACGCTTTCGCGCCGGATATTGTCGCCCAGGTCAAAAAGGC
GGTGCTCGACGAGCTGGATGGCGAGGCCGGCTGGCGCGTGGTGAGCGTCAACCAGAACGGTGTCGACACTGCGGTGCTCA
ACGAGGTGAAACCCACTCCGGCGCCGTCGAAGACGATCAGCCTTGATCGCGCCGTGCAGAACGCCGCGCAGAATGCCGTC
AATACCCGTGGCCAGAAGGCCATGATGGTAGTCATCAAACCGTCGACGGGTGAGATCCTCGCGGTCGCCCAGAACGCCGC
CGCCAACGCTGATGGACCGCTGGCCACCACCGGGTTGTATCCGCCTGGGTCGACATTCAAGATCATCACCGCCGGTGCCG
CGCTGGAGCGCGGCATGGCCACTCCGGACACCATGGTGGGCTGCCCGAAGCGGATCACCATCGGTGATCGCAGCGTGCCC
AACTACAACGAGTTCGATCTCGGCACGGTGCCCATGTGGCGGGCATTCGCGAACTCGTGCAACACCACCTTCGCGAAGCT
GGCCAGCGAGATGCCTCTGGACGGTTTGACCGTCGCCGCTTCACAATTCGGTATCGGACCCGACTACGACGTCGCGGGTA
TCCCGACCATTTCGGGCAACGTACCACCGACCGTCAATCTCACCGAGCGCACCGAGGACGGATTCGGTCAGGGCAAGGTG
CTGGTCACGCCGTTCGGCATGGCGCTGGCAGCGGCCACCGTGGCAAATGGTAAAACACCTGTACCGCAGCTGATTTCGGG
TCAGATCACCGGAATAACCGGTGAGCGGCCCGCGGTGACCCCGACCATGGTCGATGGCCTGCGCGGAATGATGCGCGAGA
CGGTGCTCAGCGGCACCGCCATGGATCTCAAGGGTGAGGGTGCGGTCTTCGGAAAAACGGGCGAAGCGGAGTTTCCGGGC
GGATCACATGCCTGGTTCGCCGGATACCGCGGCGACATGGCCTTCGCCACCTTGATCGTCGGGGGCGGCGGTTCGGAGGC
CGCGGTGCGCGCCACCAAGGTGATGTTCCAGTCGCTGCCGCCCGACTACTTGGCCTGA

Upstream 100 bases:

>100_bases
ACCTTCGCGACGGTCCTCGTCAGCTAATTTTGCGAGCTAGTGCTCGCTAGCCCCGTGCGCCTGCGTGGTTTTGGGCGTTC
GCGGTCTTAGCATCGACCCG

Downstream 100 bases:

>100_bases
TCCGCGCGCGAGGCCAGCACGCAGAATTCATTGTCCTCGGGGTCGGCCAGCACCACCCAGGGCGAATCGCCCTGGCCAAT
ATCGATCCTGGTGGCGCCGT

Product: penicillin-binding protein, putative

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MFRRGWVLAMVGTVLLVAGAVACTPRPDGPGPVAEKFFEALAKGDTAAAAKLTDDPDGAKVGLDQAFSGLQATSFKAAVN
GSQYTQDTGSADATYTWQLPRKRVWTYNGRLEMLRTAGSWQVRWAPSDLHPKLGERQMLSLRTDPAKRATVNEAGGTTVL
APANLYRIAFDASKAGKSLMSTATALADAIRPYDDTMNAASLAEQASAQTSPMDLITLRKDDWDKVSIALETRPGALRPG
VVMTPIADLLPTDDAFAPDIVAQVKKAVLDELDGEAGWRVVSVNQNGVDTAVLNEVKPTPAPSKTISLDRAVQNAAQNAV
NTRGQKAMMVVIKPSTGEILAVAQNAAANADGPLATTGLYPPGSTFKIITAGAALERGMATPDTMVGCPKRITIGDRSVP
NYNEFDLGTVPMWRAFANSCNTTFAKLASEMPLDGLTVAASQFGIGPDYDVAGIPTISGNVPPTVNLTERTEDGFGQGKV
LVTPFGMALAAATVANGKTPVPQLISGQITGITGERPAVTPTMVDGLRGMMRETVLSGTAMDLKGEGAVFGKTGEAEFPG
GSHAWFAGYRGDMAFATLIVGGGGSEAAVRATKVMFQSLPPDYLA

Sequences:

>Translated_605_residues
MFRRGWVLAMVGTVLLVAGAVACTPRPDGPGPVAEKFFEALAKGDTAAAAKLTDDPDGAKVGLDQAFSGLQATSFKAAVN
GSQYTQDTGSADATYTWQLPRKRVWTYNGRLEMLRTAGSWQVRWAPSDLHPKLGERQMLSLRTDPAKRATVNEAGGTTVL
APANLYRIAFDASKAGKSLMSTATALADAIRPYDDTMNAASLAEQASAQTSPMDLITLRKDDWDKVSIALETRPGALRPG
VVMTPIADLLPTDDAFAPDIVAQVKKAVLDELDGEAGWRVVSVNQNGVDTAVLNEVKPTPAPSKTISLDRAVQNAAQNAV
NTRGQKAMMVVIKPSTGEILAVAQNAAANADGPLATTGLYPPGSTFKIITAGAALERGMATPDTMVGCPKRITIGDRSVP
NYNEFDLGTVPMWRAFANSCNTTFAKLASEMPLDGLTVAASQFGIGPDYDVAGIPTISGNVPPTVNLTERTEDGFGQGKV
LVTPFGMALAAATVANGKTPVPQLISGQITGITGERPAVTPTMVDGLRGMMRETVLSGTAMDLKGEGAVFGKTGEAEFPG
GSHAWFAGYRGDMAFATLIVGGGGSEAAVRATKVMFQSLPPDYLA
>Mature_605_residues
MFRRGWVLAMVGTVLLVAGAVACTPRPDGPGPVAEKFFEALAKGDTAAAAKLTDDPDGAKVGLDQAFSGLQATSFKAAVN
GSQYTQDTGSADATYTWQLPRKRVWTYNGRLEMLRTAGSWQVRWAPSDLHPKLGERQMLSLRTDPAKRATVNEAGGTTVL
APANLYRIAFDASKAGKSLMSTATALADAIRPYDDTMNAASLAEQASAQTSPMDLITLRKDDWDKVSIALETRPGALRPG
VVMTPIADLLPTDDAFAPDIVAQVKKAVLDELDGEAGWRVVSVNQNGVDTAVLNEVKPTPAPSKTISLDRAVQNAAQNAV
NTRGQKAMMVVIKPSTGEILAVAQNAAANADGPLATTGLYPPGSTFKIITAGAALERGMATPDTMVGCPKRITIGDRSVP
NYNEFDLGTVPMWRAFANSCNTTFAKLASEMPLDGLTVAASQFGIGPDYDVAGIPTISGNVPPTVNLTERTEDGFGQGKV
LVTPFGMALAAATVANGKTPVPQLISGQITGITGERPAVTPTMVDGLRGMMRETVLSGTAMDLKGEGAVFGKTGEAEFPG
GSHAWFAGYRGDMAFATLIVGGGGSEAAVRATKVMFQSLPPDYLA

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=277, Percent_Identity=26.7148014440433, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786272, Length=295, Percent_Identity=26.4406779661017, Blast_Score=70, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 63298; Mature: 63298

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRGWVLAMVGTVLLVAGAVACTPRPDGPGPVAEKFFEALAKGDTAAAAKLTDDPDGAK
CCCCHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCE
VGLDQAFSGLQATSFKAAVNGSQYTQDTGSADATYTWQLPRKRVWTYNGRLEMLRTAGSW
ECHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCEEEEECCCCEEEEECCCEEEEECCCCE
QVRWAPSDLHPKLGERQMLSLRTDPAKRATVNEAGGTTVLAPANLYRIAFDASKAGKSLM
EEEECCCCCCCCCCCCCEEEECCCCCHHCCCCCCCCEEEEECCCEEEEEECCCHHHHHHH
STATALADAIRPYDDTMNAASLAEQASAQTSPMDLITLRKDDWDKVSIALETRPGALRPG
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCC
VVMTPIADLLPTDDAFAPDIVAQVKKAVLDELDGEAGWRVVSVNQNGVDTAVLNEVKPTP
EEECCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHCCCCCC
APSKTISLDRAVQNAAQNAVNTRGQKAMMVVIKPSTGEILAVAQNAAANADGPLATTGLY
CCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCEEECCCC
PPGSTFKIITAGAALERGMATPDTMVGCPKRITIGDRSVPNYNEFDLGTVPMWRAFANSC
CCCCCEEEEECCHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHCC
NTTFAKLASEMPLDGLTVAASQFGIGPDYDVAGIPTISGNVPPTVNLTERTEDGFGQGKV
CHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCE
LVTPFGMALAAATVANGKTPVPQLISGQITGITGERPAVTPTMVDGLRGMMRETVLSGTA
EEEECHHHHHHHHHCCCCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCE
MDLKGEGAVFGKTGEAEFPGGSHAWFAGYRGDMAFATLIVGGGGSEAAVRATKVMFQSLP
EEECCCCEEEECCCCCCCCCCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHCC
PDYLA
CHHCC
>Mature Secondary Structure
MFRRGWVLAMVGTVLLVAGAVACTPRPDGPGPVAEKFFEALAKGDTAAAAKLTDDPDGAK
CCCCHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCE
VGLDQAFSGLQATSFKAAVNGSQYTQDTGSADATYTWQLPRKRVWTYNGRLEMLRTAGSW
ECHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCEEEEECCCCEEEEECCCEEEEECCCCE
QVRWAPSDLHPKLGERQMLSLRTDPAKRATVNEAGGTTVLAPANLYRIAFDASKAGKSLM
EEEECCCCCCCCCCCCCEEEECCCCCHHCCCCCCCCEEEEECCCEEEEEECCCHHHHHHH
STATALADAIRPYDDTMNAASLAEQASAQTSPMDLITLRKDDWDKVSIALETRPGALRPG
HHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCC
VVMTPIADLLPTDDAFAPDIVAQVKKAVLDELDGEAGWRVVSVNQNGVDTAVLNEVKPTP
EEECCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHCCCCCC
APSKTISLDRAVQNAAQNAVNTRGQKAMMVVIKPSTGEILAVAQNAAANADGPLATTGLY
CCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEECCCCCCCCCCEEECCCC
PPGSTFKIITAGAALERGMATPDTMVGCPKRITIGDRSVPNYNEFDLGTVPMWRAFANSC
CCCCCEEEEECCHHHHCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHCC
NTTFAKLASEMPLDGLTVAASQFGIGPDYDVAGIPTISGNVPPTVNLTERTEDGFGQGKV
CHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCE
LVTPFGMALAAATVANGKTPVPQLISGQITGITGERPAVTPTMVDGLRGMMRETVLSGTA
EEEECHHHHHHHHHCCCCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCE
MDLKGEGAVFGKTGEAEFPGGSHAWFAGYRGDMAFATLIVGGGGSEAAVRATKVMFQSLP
EEECCCCEEEECCCCCCCCCCCCEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHHHCC
PDYLA
CHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 8930920 [H]