Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 169630217

GI number: 169630217

Start: 3175150

End: 3176145

Strand: Reverse

Name: nusA [H]

Synonym: MAB_3135c

Alternate gene names: 169630217

Gene position: 3176145-3175150 (Counterclockwise)

Preceding gene: 169630218

Following gene: 169630216

Centisome position: 62.68

GC content: 64.26

Gene sequence:

>996_bases
ATGAATATCGATCCCGCAGCGGTAAACCTGATGGCTGCCGATAAGGGCATCACGGTCGACGAGGCGATCGACATCATCAA
GTCGGCGCTGCTCACCGCGTATCGCCATACCGATGGGCACGAGCCCAACGCGCGTATCGAAATCGACCGCAAGACCGGTG
TGGTGCGCGTGATGGCGCGTGAGACCGATCAGGACGGCAACCTCATCACGGAGTGGGATGCCACACCCGAAGGATTCGGC
CGCATCGCGGCCACCACCGCGCGGCAGGTGTTCCAGCAGGGAGTGCGTGACGCGGAGAACGAGCACAAGTTTGGCGAGTT
CTCCACGCGTGAAGGCGAGATCGTCGGCGGCGTGATCCAGCGTGACGCCCGGGCCAACGCCCGCGGGCTCGTCGTGGTCC
GCATGGGTAGCGAGACGAAGGGCTCCGAGGGGGTCATTCCGTCGGCCGAGCAGGTGCCAGGAGAGGAGTACCACCACGGA
GACCGCCTGCGCTGCTACGTCGTTGGCGTGTCGCGGGGCGCCCGGGAGCCGTTGATCACCCTGTCGCGCACCCACCCGAA
CCTTGTACGTAAGTTGTTTTCACTGGAAGTTCCAGAGATCAGCGACGGCTCGGTAGAGATCGTCGCGGTCGCACGTGAGG
CGGGGCATCGCTCCAAGATCGCGGTGGCATCGAAGGTTTCTGGGCTCAACGCCAAGGGCGCCTGCATCGGCCCGATGGGT
CAGCGTGTGCGCAACGTGATGAGTGAGCTGTCCGGCGAGAAGATCGACATCATCGATTACGACCCGGACCCCGCGCGATT
CGTGGCGAATGCGCTGTCGCCCGCCAAGGTGGTTTCGGTTTCGGTTATCGACGAGGCGGGCAAGGCTGCCCGCGTCATTG
TGCCCGATTTCCAACTCTCGCTGGCGATCGGCAAGGAGGGGCAGAACGCCCGGCTTGCCGCGCGACTGACCGGATGGCGC
ATCGACATCAGAAGTGACGCGGACCCCGAAAACTAG

Upstream 100 bases:

>100_bases
CGTTCTCGGATATCGAGAGCGCCGTCGTGCAGGTGGAGTTCAACACGCCCAACCCGCAGGAACTCAATCTGGCTGGGGCA
GCCGAAACTGGAGGTGGCGC

Downstream 100 bases:

>100_bases
CACCGGGTAGCGGTAGGTACTGACCAAAGTCCCTATCGGCACATAACTTGCATCTCATATACAAGTTATGTGCAACTGAC
GCGATTCACCGACCTGGGCC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNIDPAAVNLMAADKGITVDEAIDIIKSALLTAYRHTDGHEPNARIEIDRKTGVVRVMARETDQDGNLITEWDATPEGFG
RIAATTARQVFQQGVRDAENEHKFGEFSTREGEIVGGVIQRDARANARGLVVVRMGSETKGSEGVIPSAEQVPGEEYHHG
DRLRCYVVGVSRGAREPLITLSRTHPNLVRKLFSLEVPEISDGSVEIVAVAREAGHRSKIAVASKVSGLNAKGACIGPMG
QRVRNVMSELSGEKIDIIDYDPDPARFVANALSPAKVVSVSVIDEAGKAARVIVPDFQLSLAIGKEGQNARLAARLTGWR
IDIRSDADPEN

Sequences:

>Translated_331_residues
MNIDPAAVNLMAADKGITVDEAIDIIKSALLTAYRHTDGHEPNARIEIDRKTGVVRVMARETDQDGNLITEWDATPEGFG
RIAATTARQVFQQGVRDAENEHKFGEFSTREGEIVGGVIQRDARANARGLVVVRMGSETKGSEGVIPSAEQVPGEEYHHG
DRLRCYVVGVSRGAREPLITLSRTHPNLVRKLFSLEVPEISDGSVEIVAVAREAGHRSKIAVASKVSGLNAKGACIGPMG
QRVRNVMSELSGEKIDIIDYDPDPARFVANALSPAKVVSVSVIDEAGKAARVIVPDFQLSLAIGKEGQNARLAARLTGWR
IDIRSDADPEN
>Mature_331_residues
MNIDPAAVNLMAADKGITVDEAIDIIKSALLTAYRHTDGHEPNARIEIDRKTGVVRVMARETDQDGNLITEWDATPEGFG
RIAATTARQVFQQGVRDAENEHKFGEFSTREGEIVGGVIQRDARANARGLVVVRMGSETKGSEGVIPSAEQVPGEEYHHG
DRLRCYVVGVSRGAREPLITLSRTHPNLVRKLFSLEVPEISDGSVEIVAVAREAGHRSKIAVASKVSGLNAKGACIGPMG
QRVRNVMSELSGEKIDIIDYDPDPARFVANALSPAKVVSVSVIDEAGKAARVIVPDFQLSLAIGKEGQNARLAARLTGWR
IDIRSDADPEN

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=342, Percent_Identity=34.5029239766082, Blast_Score=184, Evalue=6e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N [H]

EC number: NA

Molecular weight: Translated: 35701; Mature: 35701

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDPAAVNLMAADKGITVDEAIDIIKSALLTAYRHTDGHEPNARIEIDRKTGVVRVMAR
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEE
ETDQDGNLITEWDATPEGFGRIAATTARQVFQQGVRDAENEHKFGEFSTREGEIVGGVIQ
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHH
RDARANARGLVVVRMGSETKGSEGVIPSAEQVPGEEYHHGDRLRCYVVGVSRGAREPLIT
HCCCCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCHHCCCCCEEEEEEEECCCCCCCEEE
LSRTHPNLVRKLFSLEVPEISDGSVEIVAVAREAGHRSKIAVASKVSGLNAKGACIGPMG
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEEHHCCCCCCCCCEECHHH
QRVRNVMSELSGEKIDIIDYDPDPARFVANALSPAKVVSVSVIDEAGKAARVIVPDFQLS
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCEEE
LAIGKEGQNARLAARLTGWRIDIRSDADPEN
EEECCCCCCCEEEEEEEEEEEEECCCCCCCC
>Mature Secondary Structure
MNIDPAAVNLMAADKGITVDEAIDIIKSALLTAYRHTDGHEPNARIEIDRKTGVVRVMAR
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEE
ETDQDGNLITEWDATPEGFGRIAATTARQVFQQGVRDAENEHKFGEFSTREGEIVGGVIQ
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHH
RDARANARGLVVVRMGSETKGSEGVIPSAEQVPGEEYHHGDRLRCYVVGVSRGAREPLIT
HCCCCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCHHCCCCCEEEEEEEECCCCCCCEEE
LSRTHPNLVRKLFSLEVPEISDGSVEIVAVAREAGHRSKIAVASKVSGLNAKGACIGPMG
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCEEEEEHHCCCCCCCCCEECHHH
QRVRNVMSELSGEKIDIIDYDPDPARFVANALSPAKVVSVSVIDEAGKAARVIVPDFQLS
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCEEE
LAIGKEGQNARLAARLTGWRIDIRSDADPEN
EEECCCCCCCEEEEEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]