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The map label for this gene is miaA

Identifier: 169630127

GI number: 169630127

Start: 3096905

End: 3097846

Strand: Reverse

Name: miaA

Synonym: MAB_3044c

Alternate gene names: 169630127

Gene position: 3097846-3096905 (Counterclockwise)

Preceding gene: 169630128

Following gene: 169630126

Centisome position: 61.14

GC content: 66.88

Gene sequence:

>942_bases
ATGACACGGCCCATCGCGGTCATCGGTCCCACCGCCACCGGCAAGTCGGCGCTCGCGCTGGAGCTGGCCGAGCGCTTGGG
CGGGGAGATCGTCAATGCCGACGCGATGCAGCTGTATCGCGGTATGGATATCGGCACCGCCAAGGTGCCGGAATGCGAGC
GGCGGGGTCTCGTTCACCACATGCTCGACGTGCTTGACGTGACCGAAACAGCCACAGTCGCGACCTATCAAGAACGCGCC
GTCGCGACGATCGACGATATCCGTGCGCGCGGACATGTGCCGGTGATCGTCGGCGGATCGATGATGTACATCCAAGCGTT
ACTTGATGATTGGGCGTTTCCCGCGACGGACCCGCAGGTGCGTGCGCGCTGGGAGGAGCGCTTGGCGCAGATCGGGGTCA
CCGCGCTGCACGCCGAACTCGCCGCGCGTGACCCGGCGGCGGCCGCGATCATCTTGCCCACCGACGGGCGCCGCACAGTG
CGCGCGCTTGAGGTGATCGAGCTCACCGGTCAGCCGTTCGCGGCATCGGCGCCCACCATCGGCCCGCCACGGTGGGACAC
ATTGATCGTCGGATTGGATTGGGAGACGGAGAAACTCGATGATCGGATCGCCCGGCGCACGGACCTGATGTTCGAGCAGG
GTTTCGTCGGCGAGGTCGAATATCTGTTGAGTACCGGGTTGCGTGACGGTGTGACCGCGTCGCGGGCCATCGGATACGCG
CAGGTCATCGCGGCGCTGGACGCCGGGGGTGGTGCAGGGGCGCTGGCGCAGGCGCGCGACCTGACCTTCGTCGGCACCCG
CCGCTATGTCCGTCGTCAACGGTCGTGGTTCGGCCGCGATCATCGCGTGGTATGGCTGGCAGGGGAGTCCGTGGAGACCG
GTGGCCCTGAAGGGCTGGCGGGCGAAATCGTGTCGCGCTGGCGCCTATCCTCGAATACGTGA

Upstream 100 bases:

>100_bases
TGCTCGCCGGGCTGGTGGATTCCGACGTCGTCGAGGCCAGGTGCTTGTATCGCGGCTCGCCCTACGGGGTGGGGTATTTT
GCCGGCATTTGGCGGGTGTC

Downstream 100 bases:

>100_bases
AGTTCACCAAGGGGCACGGCACCCAGAACGATTTCGTGGTGCTGCCGGATGTCCACATCAAACGCGACCTGTCGGTGGCC
GCCGTCCAGGCGTTGTGCGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTRPIAVIGPTATGKSALALELAERLGGEIVNADAMQLYRGMDIGTAKVPECERRGLVHHMLDVLDVTETATVATYQERA
VATIDDIRARGHVPVIVGGSMMYIQALLDDWAFPATDPQVRARWEERLAQIGVTALHAELAARDPAAAAIILPTDGRRTV
RALEVIELTGQPFAASAPTIGPPRWDTLIVGLDWETEKLDDRIARRTDLMFEQGFVGEVEYLLSTGLRDGVTASRAIGYA
QVIAALDAGGGAGALAQARDLTFVGTRRYVRRQRSWFGRDHRVVWLAGESVETGGPEGLAGEIVSRWRLSSNT

Sequences:

>Translated_313_residues
MTRPIAVIGPTATGKSALALELAERLGGEIVNADAMQLYRGMDIGTAKVPECERRGLVHHMLDVLDVTETATVATYQERA
VATIDDIRARGHVPVIVGGSMMYIQALLDDWAFPATDPQVRARWEERLAQIGVTALHAELAARDPAAAAIILPTDGRRTV
RALEVIELTGQPFAASAPTIGPPRWDTLIVGLDWETEKLDDRIARRTDLMFEQGFVGEVEYLLSTGLRDGVTASRAIGYA
QVIAALDAGGGAGALAQARDLTFVGTRRYVRRQRSWFGRDHRVVWLAGESVETGGPEGLAGEIVSRWRLSSNT
>Mature_312_residues
TRPIAVIGPTATGKSALALELAERLGGEIVNADAMQLYRGMDIGTAKVPECERRGLVHHMLDVLDVTETATVATYQERAV
ATIDDIRARGHVPVIVGGSMMYIQALLDDWAFPATDPQVRARWEERLAQIGVTALHAELAARDPAAAAIILPTDGRRTVR
ALEVIELTGQPFAASAPTIGPPRWDTLIVGLDWETEKLDDRIARRTDLMFEQGFVGEVEYLLSTGLRDGVTASRAIGYAQ
VIAALDAGGGAGALAQARDLTFVGTRRYVRRQRSWFGRDHRVVWLAGESVETGGPEGLAGEIVSRWRLSSNT

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=34.0836012861736, Blast_Score=134, Evalue=7e-32,
Organism=Escherichia coli, GI1790613, Length=304, Percent_Identity=35.1973684210526, Blast_Score=159, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI25144712, Length=302, Percent_Identity=29.4701986754967, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=28.2312925170068, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24649830, Length=264, Percent_Identity=32.5757575757576, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_MYCA9 (B1MD01)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703776.1
- ProteinModelPortal:   B1MD01
- SMR:   B1MD01
- EnsemblBacteria:   EBMYCT00000007467
- GeneID:   5965551
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3044c
- GeneTree:   EBGT00050000016412
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- BioCyc:   MABS561007:MAB_3044C-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33921; Mature: 33790

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPIAVIGPTATGKSALALELAERLGGEIVNADAMQLYRGMDIGTAKVPECERRGLVHH
CCCCEEEECCCCCCCHHHHHHHHHHHCCEEECHHHHHHHHCCCCCCCCCCCHHHCCHHHH
MLDVLDVTETATVATYQERAVATIDDIRARGHVPVIVGGSMMYIQALLDDWAFPATDPQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCCHHH
RARWEERLAQIGVTALHAELAARDPAAAAIILPTDGRRTVRALEVIELTGQPFAASAPTI
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCC
GPPRWDTLIVGLDWETEKLDDRIARRTDLMFEQGFVGEVEYLLSTGLRDGVTASRAIGYA
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHH
QVIAALDAGGGAGALAQARDLTFVGTRRYVRRQRSWFGRDHRVVWLAGESVETGGPEGLA
HHHHHHCCCCCCCCHHHHCCEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHH
GEIVSRWRLSSNT
HHHHHHHHCCCCC
>Mature Secondary Structure 
TRPIAVIGPTATGKSALALELAERLGGEIVNADAMQLYRGMDIGTAKVPECERRGLVHH
CCCEEEECCCCCCCHHHHHHHHHHHCCEEECHHHHHHHHCCCCCCCCCCCHHHCCHHHH
MLDVLDVTETATVATYQERAVATIDDIRARGHVPVIVGGSMMYIQALLDDWAFPATDPQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCCHHH
RARWEERLAQIGVTALHAELAARDPAAAAIILPTDGRRTVRALEVIELTGQPFAASAPTI
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCC
GPPRWDTLIVGLDWETEKLDDRIARRTDLMFEQGFVGEVEYLLSTGLRDGVTASRAIGYA
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHH
QVIAALDAGGGAGALAQARDLTFVGTRRYVRRQRSWFGRDHRVVWLAGESVETGGPEGLA
HHHHHHCCCCCCCCHHHHCCEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHH
GEIVSRWRLSSNT
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA