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The map label for this gene is dapF

Identifier: 169630126

GI number: 169630126

Start: 3096021

End: 3096908

Strand: Reverse

Name: dapF

Synonym: MAB_3043c

Alternate gene names: 169630126

Gene position: 3096908-3096021 (Counterclockwise)

Preceding gene: 169630127

Following gene: 169630125

Centisome position: 61.12

GC content: 65.2

Gene sequence:

>888_bases
GTGAAGTTCACCAAGGGGCACGGCACCCAGAACGATTTCGTGGTGCTGCCGGATGTCCACATCAAACGCGACCTGTCGGT
GGCCGCCGTCCAGGCGTTGTGCGACCGGCAGCGCGGTCTCGGCGCCGATGGCGTGCTGCGCGTGACCACGGTGGGCGCTG
CTCTGGAGGGCGGGGTCCTGGCCGAGAAACCTGCCGGGGTGAGCAGCGAAGACTGGTTCATGGACTACCGCAATGCTGAC
GGTTCGATCGCCGAGATGTGCGGCAACGGTGTGCGAGTATTCGCGCACTATCTGCGATCGGTAGGACTGGAGCATCGGGA
CGAGTTTGTGGTGGCCTCGCTGGCCGGGCCCCGGCCGGTGCGCATCAATTCCTGGAGCCAGCTCACCGCCGATGTGACGG
TCGACATGGGGCCGGTCAAGGAGTTCGGTGCCGGCGAGGCGATCGTCGGTGGACGCCGGTTTTCCGGCCTGGCCATCGAC
GTGGGTAATCCACACTTGGCCTGTGTGGATGCCGTACTGACAACCGAGCAGCTGCGCATCCTCGATGTGGGTGCGCCGGT
TACCTTCGACGAGCAGTTGTTCCCCGATGGAGTCAATGTCGAGGTGCTGACGGCTCCGTCCGCGGGCCCGGCGAACACGG
TGCATATGCGCGTTCACGAGCGTGGTGTCGGCGAGACACGTTCCTGTGGAACGGGAACGGTAGCGGCGGCCTACGCCGCG
CTGCGTCACATCGGGCAGCGGACCGGCGAGGTCGTCGTCAACATTCCTGGTGGACAGGTTCGCGTGACGGTCACCGGTGA
ATCCAGCTTCCTGCGCGGGCCTTCCATGTTGCTGGCCGATGGCGAAATCTCGGATGAATGGTGGGGCGGCATTGGCGCCT
GCGGATAA

Upstream 100 bases:

>100_bases
ATCATCGCGTGGTATGGCTGGCAGGGGAGTCCGTGGAGACCGGTGGCCCTGAAGGGCTGGCGGGCGAAATCGTGTCGCGC
TGGCGCCTATCCTCGAATAC

Downstream 100 bases:

>100_bases
TTCGGATGCGTTCGATGAGCCCCGGGTGGGAGCATCGGTAGCCAGTATGCGTACCACCTATGAGACGCCCACCGACGGCG
AGCTTGCGCTGGAAGACCGC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MKFTKGHGTQNDFVVLPDVHIKRDLSVAAVQALCDRQRGLGADGVLRVTTVGAALEGGVLAEKPAGVSSEDWFMDYRNAD
GSIAEMCGNGVRVFAHYLRSVGLEHRDEFVVASLAGPRPVRINSWSQLTADVTVDMGPVKEFGAGEAIVGGRRFSGLAID
VGNPHLACVDAVLTTEQLRILDVGAPVTFDEQLFPDGVNVEVLTAPSAGPANTVHMRVHERGVGETRSCGTGTVAAAYAA
LRHIGQRTGEVVVNIPGGQVRVTVTGESSFLRGPSMLLADGEISDEWWGGIGACG

Sequences:

>Translated_295_residues
MKFTKGHGTQNDFVVLPDVHIKRDLSVAAVQALCDRQRGLGADGVLRVTTVGAALEGGVLAEKPAGVSSEDWFMDYRNAD
GSIAEMCGNGVRVFAHYLRSVGLEHRDEFVVASLAGPRPVRINSWSQLTADVTVDMGPVKEFGAGEAIVGGRRFSGLAID
VGNPHLACVDAVLTTEQLRILDVGAPVTFDEQLFPDGVNVEVLTAPSAGPANTVHMRVHERGVGETRSCGTGTVAAAYAA
LRHIGQRTGEVVVNIPGGQVRVTVTGESSFLRGPSMLLADGEISDEWWGGIGACG
>Mature_295_residues
MKFTKGHGTQNDFVVLPDVHIKRDLSVAAVQALCDRQRGLGADGVLRVTTVGAALEGGVLAEKPAGVSSEDWFMDYRNAD
GSIAEMCGNGVRVFAHYLRSVGLEHRDEFVVASLAGPRPVRINSWSQLTADVTVDMGPVKEFGAGEAIVGGRRFSGLAID
VGNPHLACVDAVLTTEQLRILDVGAPVTFDEQLFPDGVNVEVLTAPSAGPANTVHMRVHERGVGETRSCGTGTVAAAYAA
LRHIGQRTGEVVVNIPGGQVRVTVTGESSFLRGPSMLLADGEISDEWWGGIGACG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=297, Percent_Identity=30.6397306397306, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_MYCA9 (B1MD00)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703775.1
- ProteinModelPortal:   B1MD00
- SMR:   B1MD00
- EnsemblBacteria:   EBMYCT00000006717
- GeneID:   5965550
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3043c
- GeneTree:   EBGT00050000017180
- HOGENOM:   HBG399442
- OMA:   NACGTAM
- ProtClustDB:   PRK00450
- BioCyc:   MABS561007:MAB_3043C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31029; Mature: 31029

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 87-87 ACT_SITE 229-229

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTKGHGTQNDFVVLPDVHIKRDLSVAAVQALCDRQRGLGADGVLRVTTVGAALEGGVL
CCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHCCCEE
AEKPAGVSSEDWFMDYRNADGSIAEMCGNGVRVFAHYLRSVGLEHRDEFVVASLAGPRPV
ECCCCCCCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCE
RINSWSQLTADVTVDMGPVKEFGAGEAIVGGRRFSGLAIDVGNPHLACVDAVLTTEQLRI
EECCHHHEEEEEEEECCCCHHCCCCCEEECCCEECCEEEECCCCCEEEHHHHHHHCEEEE
LDVGAPVTFDEQLFPDGVNVEVLTAPSAGPANTVHMRVHERGVGETRSCGTGTVAAAYAA
EECCCCCCCCHHHCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHH
LRHIGQRTGEVVVNIPGGQVRVTVTGESSFLRGPSMLLADGEISDEWWGGIGACG
HHHHHCCCCCEEEECCCCEEEEEEECCCCCCCCCCEEEECCCCCHHHCCCCCCCC
>Mature Secondary Structure
MKFTKGHGTQNDFVVLPDVHIKRDLSVAAVQALCDRQRGLGADGVLRVTTVGAALEGGVL
CCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHCCCEE
AEKPAGVSSEDWFMDYRNADGSIAEMCGNGVRVFAHYLRSVGLEHRDEFVVASLAGPRPV
ECCCCCCCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCE
RINSWSQLTADVTVDMGPVKEFGAGEAIVGGRRFSGLAIDVGNPHLACVDAVLTTEQLRI
EECCHHHEEEEEEEECCCCHHCCCCCEEECCCEECCEEEECCCCCEEEHHHHHHHCEEEE
LDVGAPVTFDEQLFPDGVNVEVLTAPSAGPANTVHMRVHERGVGETRSCGTGTVAAAYAA
EECCCCCCCCHHHCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHH
LRHIGQRTGEVVVNIPGGQVRVTVTGESSFLRGPSMLLADGEISDEWWGGIGACG
HHHHHCCCCCEEEECCCCEEEEEEECCCCCCCCCCEEEECCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA