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The map label for this gene is sigB [H]

Identifier: 169630111

GI number: 169630111

Start: 3080141

End: 3081112

Strand: Direct

Name: sigB [H]

Synonym: MAB_3028

Alternate gene names: 169630111

Gene position: 3080141-3081112 (Clockwise)

Preceding gene: 169630110

Following gene: 169630112

Centisome position: 60.79

GC content: 65.12

Gene sequence:

>972_bases
ATGGCAAACGCCACCACCCGTCCGGCCCGCACCACCGAGTCCGAGGATCTGGACGCCCAGAGTCCAGCCGCCGATCTCGT
TCGGGTGTATTTGAACGGCATTGGCAAGACCGCCCTGCTCACAGCGGAGGACGAAGTCGAGCTGGCCAAACGGATTGAGG
CGGGGCTCTATGCCCAGCACCTTCTCGACACCAAGAAGCGTCTTGGCGAGGCCCGCAAGAAGGACCTGGCCGTGATCGTG
CGGGAAGGCAACGCAGCGCGCAGCCATCTGCTGGAAGCGAACCTGCGTCTGGTGGTCTCGCTCGCCAAGCGCTACACCGG
CCGCGGTATGCCCCTGCTGGATCTCATCCAGGAAGGCAACCTGGGCTTGATCCGCGCCATGGAGAAGTTCGACTACGCCA
AGGGCTTCAAGTTCTCGACGTACGCCACGTGGTGGATCCGCCAGGCCATCACCCGCGGCATGGCCGACCAGAGCCGCACC
ATCCGCCTGCCCGTGCATCTGGTGGAGCAGGTGAACAAGTTGGCACGCATCAAGCGCGAGCTGCACCAGCGGCTCGGCCG
CGAGGCCAGCGACGAGGAACTTGCCGAAGAGTCCGGCATCCCGGTGGAAAAGATCGGCGACCTGCTGGACCACAGCCGTG
ATCCGGTGAGCCTGGATATGCCGGTCGGTACCGACGAGGAAGCACCGCTGGGTGATTTCATCGAGGACACCGAGGCCATG
TCGGCGGAGAATGCCGTCATCGCTGAGCTGCTGCACTCGGATGTGCGCAGCGTGCTGGCCACGCTCGATGAACGCGAACA
GCAGGTCATTCGGCTGCGGTACGGCCTCGATGACGGACAGGCGCGGACGCTGGATCAGATCGGCAAGCTCTTCGGGCTCT
CCCGCGAGCGGGTGCGCCAGATCGAGCGTGAGGTCATGGCGAAACTGCGCCACGGCGACCGCGCGGAGCGCCTGCGTTCG
TACGCGAGCTAG

Upstream 100 bases:

>100_bases
TCCGCAGGTCAATGACGGCGATGTTCGGGAACCTCAGGAACTTTCTGGGGCCACCGGGCGTTGCAACACATGAGACTTAC
GGATCAACTAGGAGGCAGCG

Downstream 100 bases:

>100_bases
TGGACCTACTACCCTGCCCTCGGGGCGCTGCGTTCTGGAAGCGCAGCGCCCCGATTTACGTTGTAGGTCGGTAGACTGAG
CACGACCGTAAGGGGCGAGT

Product: RNA polymerase sigma factor SigB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MANATTRPARTTESEDLDAQSPAADLVRVYLNGIGKTALLTAEDEVELAKRIEAGLYAQHLLDTKKRLGEARKKDLAVIV
REGNAARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYAKGFKFSTYATWWIRQAITRGMADQSRT
IRLPVHLVEQVNKLARIKRELHQRLGREASDEELAEESGIPVEKIGDLLDHSRDPVSLDMPVGTDEEAPLGDFIEDTEAM
SAENAVIAELLHSDVRSVLATLDEREQQVIRLRYGLDDGQARTLDQIGKLFGLSRERVRQIEREVMAKLRHGDRAERLRS
YAS

Sequences:

>Translated_323_residues
MANATTRPARTTESEDLDAQSPAADLVRVYLNGIGKTALLTAEDEVELAKRIEAGLYAQHLLDTKKRLGEARKKDLAVIV
REGNAARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYAKGFKFSTYATWWIRQAITRGMADQSRT
IRLPVHLVEQVNKLARIKRELHQRLGREASDEELAEESGIPVEKIGDLLDHSRDPVSLDMPVGTDEEAPLGDFIEDTEAM
SAENAVIAELLHSDVRSVLATLDEREQQVIRLRYGLDDGQARTLDQIGKLFGLSRERVRQIEREVMAKLRHGDRAERLRS
YAS
>Mature_322_residues
ANATTRPARTTESEDLDAQSPAADLVRVYLNGIGKTALLTAEDEVELAKRIEAGLYAQHLLDTKKRLGEARKKDLAVIVR
EGNAARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYAKGFKFSTYATWWIRQAITRGMADQSRTI
RLPVHLVEQVNKLARIKRELHQRLGREASDEELAEESGIPVEKIGDLLDHSRDPVSLDMPVGTDEEAPLGDFIEDTEAMS
AENAVIAELLHSDVRSVLATLDEREQQVIRLRYGLDDGQARTLDQIGKLFGLSRERVRQIEREVMAKLRHGDRAERLRSY
AS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=271, Percent_Identity=48.7084870848708, Blast_Score=272, Evalue=2e-74,
Organism=Escherichia coli, GI1789098, Length=287, Percent_Identity=41.4634146341463, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1789871, Length=238, Percent_Identity=31.0924369747899, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788231, Length=203, Percent_Identity=30.5418719211823, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36287; Mature: 36156

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANATTRPARTTESEDLDAQSPAADLVRVYLNGIGKTALLTAEDEVELAKRIEAGLYAQH
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHH
LLDTKKRLGEARKKDLAVIVREGNAARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGN
HHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
LGLIRAMEKFDYAKGFKFSTYATWWIRQAITRGMADQSRTIRLPVHLVEQVNKLARIKRE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHH
LHQRLGREASDEELAEESGIPVEKIGDLLDHSRDPVSLDMPVGTDEEAPLGDFIEDTEAM
HHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHH
SAENAVIAELLHSDVRSVLATLDEREQQVIRLRYGLDDGQARTLDQIGKLFGLSRERVRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHH
IEREVMAKLRHGDRAERLRSYAS
HHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
ANATTRPARTTESEDLDAQSPAADLVRVYLNGIGKTALLTAEDEVELAKRIEAGLYAQH
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHH
LLDTKKRLGEARKKDLAVIVREGNAARSHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGN
HHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCC
LGLIRAMEKFDYAKGFKFSTYATWWIRQAITRGMADQSRTIRLPVHLVEQVNKLARIKRE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHH
LHQRLGREASDEELAEESGIPVEKIGDLLDHSRDPVSLDMPVGTDEEAPLGDFIEDTEAM
HHHHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHH
SAENAVIAELLHSDVRSVLATLDEREQQVIRLRYGLDDGQARTLDQIGKLFGLSRERVRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHH
IEREVMAKLRHGDRAERLRSYAS
HHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910; 2116013 [H]