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The map label for this gene is dkgA [H]

Identifier: 169630024

GI number: 169630024

Start: 2997407

End: 2998297

Strand: Direct

Name: dkgA [H]

Synonym: MAB_2941

Alternate gene names: 169630024

Gene position: 2997407-2998297 (Clockwise)

Preceding gene: 169630023

Following gene: 169630025

Centisome position: 59.15

GC content: 60.72

Gene sequence:

>891_bases
GTGCATCCATGCACTGTCCCCACAGTTCGCTTCCATAACGGTGTCGACATCCCCCAGCTGGGCCTCGGTGTCGCCGCCAT
CCCCGACGCAGAAGCGGAAACCATTGTCCGGCAAGCACTTGATGCCGGGTATCGGCATATCGATACCGCGACCAGATACG
GCAACGAGCGCGGCGTCGGCCTCGGCATCTCTCGCTCGGGTGTATGCCGGGAAGAAGTTTTCGTCACCACGAAGCTATGG
GTAGACGATTTCCGAGATGCCGCCAGCGCTTTCGCGCGGAGCCTTGAGGCGCTCGGCACCGACTATGTGGATCTATTCCT
CATTCATTGGCCCGCACCGGATTACGGACAGTATGTGACGGCGTGGCAGCAGATTGTGCAGCTGTATCTCTCCGGCGACA
TCCGCGCCATCGGCGTATCCAACTTCGAACCTGAGCACGTGCAAGCCATCGTGGACGCGACCGGCGTGCTGCCGGTGATC
AACCAGGTGGAACTACATCCGTACTTCCAGCAGCGAAAGTTGCGGCAGTTCAACTCAGATCGGGAAATCGTCACCGAGGC
CTGGAGTCCCCTTGCCCAGGGCATCGGTACCGCCGAGGACTCGCCGCTGGCGGCGCTGGCCGCCAAGCACAACAAGACAC
CGGCTCAGCTGATCCTGCGTTGGCATATCGAGCTAGGACATGTGGTGATCCCGAAGACATGCTCGCCCCAACGGTTGACG
GAGAATCTGCAGATCTTCGATTTCAGTTTGTCCCCGGAAGATCTGGACGTGTTCGCTCAGTTCGACCAGCCCAACGGCAG
ACTCGGAACACACCCCAACGAAGGATTCTCGGCGCACCATTCGGCGCGCAGTAGATGGCATCGGGACCGGCTGGCCAGAC
GAGCCGCCTAG

Upstream 100 bases:

>100_bases
TTTCAACTGACAGTTCTACAGTTTCAACTGTCACAAGGACGTGTCGCTCCCGGGTATCAATTCCACTGCTCCTTCAGCCG
CAGAGTGATTGGAGTCCGTA

Downstream 100 bases:

>100_bases
TCAGCGGCCCGGGCTTCTTGCGGTCGCGCGGACGTGTTGACCGGTCCGCGCCGGCGCCCCCGCATCACATCAATAGAATC
GGTGGCCGTGGCTGAACAAG

Product: putative oxidoreductase

Products: NA

Alternate protein names: 2,5-DKG reductase A; 2,5-DKGR A; 25DKGR-A; AKR5C [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MHPCTVPTVRFHNGVDIPQLGLGVAAIPDAEAETIVRQALDAGYRHIDTATRYGNERGVGLGISRSGVCREEVFVTTKLW
VDDFRDAASAFARSLEALGTDYVDLFLIHWPAPDYGQYVTAWQQIVQLYLSGDIRAIGVSNFEPEHVQAIVDATGVLPVI
NQVELHPYFQQRKLRQFNSDREIVTEAWSPLAQGIGTAEDSPLAALAAKHNKTPAQLILRWHIELGHVVIPKTCSPQRLT
ENLQIFDFSLSPEDLDVFAQFDQPNGRLGTHPNEGFSAHHSARSRWHRDRLARRAA

Sequences:

>Translated_296_residues
MHPCTVPTVRFHNGVDIPQLGLGVAAIPDAEAETIVRQALDAGYRHIDTATRYGNERGVGLGISRSGVCREEVFVTTKLW
VDDFRDAASAFARSLEALGTDYVDLFLIHWPAPDYGQYVTAWQQIVQLYLSGDIRAIGVSNFEPEHVQAIVDATGVLPVI
NQVELHPYFQQRKLRQFNSDREIVTEAWSPLAQGIGTAEDSPLAALAAKHNKTPAQLILRWHIELGHVVIPKTCSPQRLT
ENLQIFDFSLSPEDLDVFAQFDQPNGRLGTHPNEGFSAHHSARSRWHRDRLARRAA
>Mature_296_residues
MHPCTVPTVRFHNGVDIPQLGLGVAAIPDAEAETIVRQALDAGYRHIDTATRYGNERGVGLGISRSGVCREEVFVTTKLW
VDDFRDAASAFARSLEALGTDYVDLFLIHWPAPDYGQYVTAWQQIVQLYLSGDIRAIGVSNFEPEHVQAIVDATGVLPVI
NQVELHPYFQQRKLRQFNSDREIVTEAWSPLAQGIGTAEDSPLAALAAKHNKTPAQLILRWHIELGHVVIPKTCSPQRLT
ENLQIFDFSLSPEDLDVFAQFDQPNGRLGTHPNEGFSAHHSARSRWHRDRLARRAA

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=290, Percent_Identity=35.1724137931034, Blast_Score=177, Evalue=8e-45,
Organism=Homo sapiens, GI5453543, Length=287, Percent_Identity=35.8885017421603, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI93277124, Length=305, Percent_Identity=31.4754098360656, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI310109922, Length=269, Percent_Identity=38.2899628252788, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI310109920, Length=287, Percent_Identity=35.8885017421603, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI45446745, Length=287, Percent_Identity=35.8885017421603, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI4503285, Length=287, Percent_Identity=35.8885017421603, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI24497583, Length=320, Percent_Identity=33.75, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI24497577, Length=290, Percent_Identity=31.7241379310345, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI5174391, Length=290, Percent_Identity=31.7241379310345, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI24497585, Length=317, Percent_Identity=34.384858044164, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI4502049, Length=288, Percent_Identity=35.4166666666667, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI291291012, Length=263, Percent_Identity=34.9809885931559, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI5174695, Length=287, Percent_Identity=34.4947735191638, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=33.2129963898917, Blast_Score=142, Evalue=4e-34,
Organism=Homo sapiens, GI300116271, Length=267, Percent_Identity=33.7078651685393, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI310109924, Length=137, Percent_Identity=39.4160583941606, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI310109926, Length=112, Percent_Identity=41.9642857142857, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI207028673, Length=113, Percent_Identity=41.5929203539823, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87082198, Length=270, Percent_Identity=47.4074074074074, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1786400, Length=256, Percent_Identity=38.671875, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1788081, Length=283, Percent_Identity=26.8551236749117, Blast_Score=84, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI71998625, Length=270, Percent_Identity=38.5185185185185, Blast_Score=198, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17537077, Length=286, Percent_Identity=37.0629370629371, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17537075, Length=314, Percent_Identity=35.3503184713376, Blast_Score=168, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17566692, Length=287, Percent_Identity=35.191637630662, Blast_Score=163, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17538386, Length=275, Percent_Identity=33.0909090909091, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17564128, Length=290, Percent_Identity=35.8620689655172, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17550248, Length=312, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17561298, Length=271, Percent_Identity=30.9963099630996, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17562292, Length=275, Percent_Identity=31.6363636363636, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17561300, Length=252, Percent_Identity=31.3492063492063, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17552492, Length=262, Percent_Identity=30.5343511450382, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17537079, Length=288, Percent_Identity=30.2083333333333, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324694, Length=295, Percent_Identity=36.271186440678, Blast_Score=186, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6320576, Length=288, Percent_Identity=35.0694444444444, Blast_Score=184, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319625, Length=302, Percent_Identity=34.7682119205298, Blast_Score=158, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6322556, Length=264, Percent_Identity=32.1969696969697, Blast_Score=155, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6320079, Length=304, Percent_Identity=29.6052631578947, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321896, Length=297, Percent_Identity=30.6397306397306, Blast_Score=119, Evalue=4e-28,
Organism=Drosophila melanogaster, GI20129731, Length=303, Percent_Identity=37.953795379538, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24644950, Length=294, Percent_Identity=36.0544217687075, Blast_Score=172, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24657054, Length=305, Percent_Identity=34.0983606557377, Blast_Score=170, Evalue=9e-43,
Organism=Drosophila melanogaster, GI21356425, Length=290, Percent_Identity=33.7931034482759, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI45553081, Length=287, Percent_Identity=36.9337979094077, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24663317, Length=288, Percent_Identity=34.7222222222222, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI221378297, Length=314, Percent_Identity=33.7579617834395, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24662781, Length=309, Percent_Identity=35.5987055016181, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24662789, Length=315, Percent_Identity=32.0634920634921, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24662785, Length=306, Percent_Identity=35.2941176470588, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI281366140, Length=251, Percent_Identity=36.6533864541833, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 33014; Mature: 33014

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPCTVPTVRFHNGVDIPQLGLGVAAIPDAEAETIVRQALDAGYRHIDTATRYGNERGVG
CCCCCCCEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
LGISRSGVCREEVFVTTKLWVDDFRDAASAFARSLEALGTDYVDLFLIHWPAPDYGQYVT
ECCCCCCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
AWQQIVQLYLSGDIRAIGVSNFEPEHVQAIVDATGVLPVINQVELHPYFQQRKLRQFNSD
HHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHCCCC
REIVTEAWSPLAQGIGTAEDSPLAALAAKHNKTPAQLILRWHIELGHVVIPKTCSPQRLT
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHEEEHEEEEECCEEECCCCCHHHHH
ENLQIFDFSLSPEDLDVFAQFDQPNGRLGTHPNEGFSAHHSARSRWHRDRLARRAA
CCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHPCTVPTVRFHNGVDIPQLGLGVAAIPDAEAETIVRQALDAGYRHIDTATRYGNERGVG
CCCCCCCEEEECCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
LGISRSGVCREEVFVTTKLWVDDFRDAASAFARSLEALGTDYVDLFLIHWPAPDYGQYVT
ECCCCCCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
AWQQIVQLYLSGDIRAIGVSNFEPEHVQAIVDATGVLPVINQVELHPYFQQRKLRQFNSD
HHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHCCCC
REIVTEAWSPLAQGIGTAEDSPLAALAAKHNKTPAQLILRWHIELGHVVIPKTCSPQRLT
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHEEEHEEEEECCEEECCCCCHHHHH
ENLQIFDFSLSPEDLDVFAQFDQPNGRLGTHPNEGFSAHHSARSRWHRDRLARRAA
CCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]