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The map label for this gene is metK [H]

Identifier: 169629904

GI number: 169629904

Start: 2870101

End: 2871267

Strand: Reverse

Name: metK [H]

Synonym: MAB_2820c

Alternate gene names: 169629904

Gene position: 2871267-2870101 (Counterclockwise)

Preceding gene: 169629905

Following gene: 169629902

Centisome position: 56.66

GC content: 65.64

Gene sequence:

>1167_bases
GTGACCGAAGGGCATCCGGACAAGATCTGTGACGCCATCAGTGACTCGATCCTGGACGCCATGCTCGCGCAGGACCCGAA
GTCCCGGGTGGCGGTGGAGACGCTGGTGACCACCGGTCAGGTCCATGTCGCCGGCGAGGTGACTACCTCCGCCTATGTCG
ATATCCCGCACATCGTCCGGGAAAGAGTGCTGGAAATCGGCTATGACTCCTCTTCCAAGGGCTTCGATGGAAACTCCTGT
GGTGTGAATGTCGCCATCGGTGCGCAGTCACCCGATATCGCGCAAGGTGTCGACACGGCCCACGAGGCCCGCGTTGAGGG
CGCCGCGGATCCGCTGGACGCTCAGGGAGCCGGCGATCAGGGCCTGATGTTCGGCTACGCCATCAACGACACCCCCGAGC
TGATGCCGCTGCCCATCGCGCTGGCACACCGGCTCTCGCGCCGCCTGACTGAGGTGCGCAAGGATGGGCTGCTGCCGTAC
CTGCGTCCCGACGGTAAGACTCAGGTCACCATCGAGTATGACGGCAACAAGGCCGTCCGGCTGGACACTGTCGTGCTCTC
CACGCAGCACGCCGCGGATATCGATCTCGAAAATCTGCTGACCCCGGACATCCGCGAGAAGGTGGTCCAGGTAGTGCTCG
ACGAGCTGAACCAGGATGGTCTGGACATCTCCGACTACCGGCTGCTGGTCAACCCGACGGGCAAGTTCGTGCTCGGCGGG
CCGATGGGTGACGCCGGACTGACCGGCCGCAAGATCATCGTCGACACCTACGGCGGCTGGGCCCGCCACGGCGGCGGCGC
GTTCTCGGGCAAGGACCCGTCAAAGGTGGACCGTTCCGCCGCGTACGCGATGCGCTGGGTGGCCAAGAATGTCGTCGCGG
CCGGCCTCGCCGACCGGGCCGAGGTGCAGGTCGCGTACGCCATCGGCAAGGCAGCGCCGGTCGGTCTGTTCGTTGACACG
TTCGGTACCGCCACCGTCGATCAGGCCAAGATTGAGAAGGCCATCAACGAGGTGTTCGACCTGCGTCCCGGCGCCATCAT
CCGCGACCTGGACCTCCTGCGTCCCATCTACGCGCAGACCGCCGCGTACGGCCACTTCGGCCGCACCGATGTCGAGCTGC
CCTGGGAACAGACCGACCGCGTCGATGCGCTCAAGGCCGCCATTTAG

Upstream 100 bases:

>100_bases
GCCTCGATCTTGGTTCGTCGGCCATAATGATTAGGACATGTAACGATCTGAAAGGGGCTGCGCTGTGAGCTCAGCTGGAC
GGCTTTTCACCAGCGAATCA

Downstream 100 bases:

>100_bases
CCGCTGCGCTTCCTGCCGAGCCGACGCAAAGCCCCCGACACGCCGAGTGCGGGGGCTTTTGCGTCGGCTCGGCGCCTAGA
TGATCTTCGGGATCAGCGCC

Product: S-adenosylmethionine synthetase (methionine adenosyltransferase)

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MTEGHPDKICDAISDSILDAMLAQDPKSRVAVETLVTTGQVHVAGEVTTSAYVDIPHIVRERVLEIGYDSSSKGFDGNSC
GVNVAIGAQSPDIAQGVDTAHEARVEGAADPLDAQGAGDQGLMFGYAINDTPELMPLPIALAHRLSRRLTEVRKDGLLPY
LRPDGKTQVTIEYDGNKAVRLDTVVLSTQHAADIDLENLLTPDIREKVVQVVLDELNQDGLDISDYRLLVNPTGKFVLGG
PMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLADRAEVQVAYAIGKAAPVGLFVDT
FGTATVDQAKIEKAINEVFDLRPGAIIRDLDLLRPIYAQTAAYGHFGRTDVELPWEQTDRVDALKAAI

Sequences:

>Translated_388_residues
MTEGHPDKICDAISDSILDAMLAQDPKSRVAVETLVTTGQVHVAGEVTTSAYVDIPHIVRERVLEIGYDSSSKGFDGNSC
GVNVAIGAQSPDIAQGVDTAHEARVEGAADPLDAQGAGDQGLMFGYAINDTPELMPLPIALAHRLSRRLTEVRKDGLLPY
LRPDGKTQVTIEYDGNKAVRLDTVVLSTQHAADIDLENLLTPDIREKVVQVVLDELNQDGLDISDYRLLVNPTGKFVLGG
PMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLADRAEVQVAYAIGKAAPVGLFVDT
FGTATVDQAKIEKAINEVFDLRPGAIIRDLDLLRPIYAQTAAYGHFGRTDVELPWEQTDRVDALKAAI
>Mature_387_residues
TEGHPDKICDAISDSILDAMLAQDPKSRVAVETLVTTGQVHVAGEVTTSAYVDIPHIVRERVLEIGYDSSSKGFDGNSCG
VNVAIGAQSPDIAQGVDTAHEARVEGAADPLDAQGAGDQGLMFGYAINDTPELMPLPIALAHRLSRRLTEVRKDGLLPYL
RPDGKTQVTIEYDGNKAVRLDTVVLSTQHAADIDLENLLTPDIREKVVQVVLDELNQDGLDISDYRLLVNPTGKFVLGGP
MGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLADRAEVQVAYAIGKAAPVGLFVDTF
GTATVDQAKIEKAINEVFDLRPGAIIRDLDLLRPIYAQTAAYGHFGRTDVELPWEQTDRVDALKAAI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=54.2105263157895, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=53.9473684210526, Blast_Score=371, Evalue=1e-103,
Organism=Escherichia coli, GI1789311, Length=387, Percent_Identity=60.4651162790698, Blast_Score=455, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17538494, Length=381, Percent_Identity=54.8556430446194, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25145633, Length=385, Percent_Identity=54.8051948051948, Blast_Score=381, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17538498, Length=381, Percent_Identity=54.5931758530184, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17551082, Length=381, Percent_Identity=50.3937007874016, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI25145635, Length=345, Percent_Identity=53.9130434782609, Blast_Score=324, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI32565911, Length=341, Percent_Identity=53.6656891495601, Blast_Score=322, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI32565905, Length=382, Percent_Identity=48.9528795811518, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI32565903, Length=317, Percent_Identity=52.9968454258675, Blast_Score=296, Evalue=9e-81,
Organism=Caenorhabditis elegans, GI32565909, Length=76, Percent_Identity=53.9473684210526, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320710, Length=387, Percent_Identity=54.7803617571059, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6323209, Length=382, Percent_Identity=56.282722513089, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI45552159, Length=379, Percent_Identity=51.7150395778364, Blast_Score=350, Evalue=8e-97,
Organism=Drosophila melanogaster, GI24580529, Length=379, Percent_Identity=51.7150395778364, Blast_Score=350, Evalue=8e-97,
Organism=Drosophila melanogaster, GI24580537, Length=379, Percent_Identity=51.7150395778364, Blast_Score=350, Evalue=8e-97,
Organism=Drosophila melanogaster, GI24580533, Length=379, Percent_Identity=51.7150395778364, Blast_Score=350, Evalue=8e-97,
Organism=Drosophila melanogaster, GI24580535, Length=379, Percent_Identity=51.7150395778364, Blast_Score=350, Evalue=8e-97,
Organism=Drosophila melanogaster, GI24580531, Length=379, Percent_Identity=51.7150395778364, Blast_Score=350, Evalue=8e-97,
Organism=Drosophila melanogaster, GI24580527, Length=379, Percent_Identity=51.7150395778364, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580525, Length=379, Percent_Identity=51.7150395778364, Blast_Score=346, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24580541, Length=375, Percent_Identity=50.6666666666667, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24580539, Length=108, Percent_Identity=54.6296296296296, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41523; Mature: 41392

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEGHPDKICDAISDSILDAMLAQDPKSRVAVETLVTTGQVHVAGEVTTSAYVDIPHIVR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCEEECCHHHHH
ERVLEIGYDSSSKGFDGNSCGVNVAIGAQSPDIAQGVDTAHEARVEGAADPLDAQGAGDQ
HHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCHHHHHHCCCCCCCCCCCCCCCC
GLMFGYAINDTPELMPLPIALAHRLSRRLTEVRKDGLLPYLRPDGKTQVTIEYDGNKAVR
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEECCCCEEE
LDTVVLSTQHAADIDLENLLTPDIREKVVQVVLDELNQDGLDISDYRLLVNPTGKFVLGG
EEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEC
PMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLADRA
CCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EVQVAYAIGKAAPVGLFVDTFGTATVDQAKIEKAINEVFDLRPGAIIRDLDLLRPIYAQT
HEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AAYGHFGRTDVELPWEQTDRVDALKAAI
HHCCCCCCCCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
TEGHPDKICDAISDSILDAMLAQDPKSRVAVETLVTTGQVHVAGEVTTSAYVDIPHIVR
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCEEECCHHHHH
ERVLEIGYDSSSKGFDGNSCGVNVAIGAQSPDIAQGVDTAHEARVEGAADPLDAQGAGDQ
HHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCHHHHHHCCCCCCCCCCCCCCCC
GLMFGYAINDTPELMPLPIALAHRLSRRLTEVRKDGLLPYLRPDGKTQVTIEYDGNKAVR
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEECCCCEEE
LDTVVLSTQHAADIDLENLLTPDIREKVVQVVLDELNQDGLDISDYRLLVNPTGKFVLGG
EEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEC
PMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNVVAAGLADRA
CCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EVQVAYAIGKAAPVGLFVDTFGTATVDQAKIEKAINEVFDLRPGAIIRDLDLLRPIYAQT
HEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AAYGHFGRTDVELPWEQTDRVDALKAAI
HHCCCCCCCCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA