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The map label for this gene is 169629902

Identifier: 169629902

GI number: 169629902

Start: 2868217

End: 2869065

Strand: Reverse

Name: 169629902

Synonym: MAB_2818c

Alternate gene names: NA

Gene position: 2869065-2868217 (Counterclockwise)

Preceding gene: 169629904

Following gene: 169629901

Centisome position: 56.62

GC content: 61.25

Gene sequence:

>849_bases
ATGACGGTGATCAGAGCCGCACTGACGCAAGCGACTTGGACCGGCGACAAGGAATCGATGCTCGCCAAACACGAGCGTTT
TGTCGCCCAGGCTGCGGCCCGGGGTGCCCAGGTGATCTGCTTTCAAGAGCTGTTCTACGGCCCGTATTTCGGCATCGTCC
AGGACAAGAAGTACTACGGCTACGCCGAACCCGTGCCCGGCCCGGTTACCGAGCGATTCGCCAAGTTGGCCAGGGAGCAC
CGCATGGTCCTGGTGCTGCCCGTGTACGAGCAGGAACAACCGGGCGTTCTCTACAACACCGCCGCCGTCGTCGATTCCGA
TGGCAGATACCTGGGCAAGTACCGTAAGCACCATATTCCGCACCTGGACCGGTTCTGGGAGAAGTTCTACTTCCGGCCGG
GCAATCTCGGATACCCGGTGTTCGACACCGCCGTCGGCAAGATCGGCGTCTACATCTGTTATGACCGCCATTTCCCCGAG
GGCTGGCGCAACTACGGACTGGCCGGAGCAGAGCTGGTCTTCAATCCGTCGGCCACCAAACCTGGTCTTTCCAACAGGCT
TTGGGAACTCGAGCAGCCCGCCGCCGCGGCGGCGAACCAGTACTTCGTCGGCGCCAACAATCGGATAGGCGCCGAAAGCG
GTGAATTCGGCGATCAGGCGGTCAGCTTCTATGGCAGTTCGTACTTTGTGGACCCGCGCGGCAACTATGTCGGCGAAATC
GGCTCCGCGACCGACGAAGAGATCGTGATACGGGATCTCGACCTGTCGCTGGTCCGTCAAGTTCGGGATGACTGGCAGTT
TTACCGGGACCGCCGTCCGGATACCTACGGCCCGATCATCGCACCGTAG

Upstream 100 bases:

>100_bases
GCCCGGAGGGCACCCTGGATACATGCCCGGCGGTTGGGCACCGGGCGCCCGTTGAACCGTTTGGCGCGCGCCAACGGCGC
AGTGCGCATAAAGTCGGTCA

Downstream 100 bases:

>100_bases
GAGGCTGCGATGGCAACGACTTTGATCCGGGGCGGCACGGTGGTGTCGGCGACCGGACGCGGTGCCGCCGATGTCTTGGT
GGACGGCGCGACGATCGCGG

Product: putative hydrolase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTVIRAALTQATWTGDKESMLAKHERFVAQAAARGAQVICFQELFYGPYFGIVQDKKYYGYAEPVPGPVTERFAKLAREH
RMVLVLPVYEQEQPGVLYNTAAVVDSDGRYLGKYRKHHIPHLDRFWEKFYFRPGNLGYPVFDTAVGKIGVYICYDRHFPE
GWRNYGLAGAELVFNPSATKPGLSNRLWELEQPAAAAANQYFVGANNRIGAESGEFGDQAVSFYGSSYFVDPRGNYVGEI
GSATDEEIVIRDLDLSLVRQVRDDWQFYRDRRPDTYGPIIAP

Sequences:

>Translated_282_residues
MTVIRAALTQATWTGDKESMLAKHERFVAQAAARGAQVICFQELFYGPYFGIVQDKKYYGYAEPVPGPVTERFAKLAREH
RMVLVLPVYEQEQPGVLYNTAAVVDSDGRYLGKYRKHHIPHLDRFWEKFYFRPGNLGYPVFDTAVGKIGVYICYDRHFPE
GWRNYGLAGAELVFNPSATKPGLSNRLWELEQPAAAAANQYFVGANNRIGAESGEFGDQAVSFYGSSYFVDPRGNYVGEI
GSATDEEIVIRDLDLSLVRQVRDDWQFYRDRRPDTYGPIIAP
>Mature_281_residues
TVIRAALTQATWTGDKESMLAKHERFVAQAAARGAQVICFQELFYGPYFGIVQDKKYYGYAEPVPGPVTERFAKLAREHR
MVLVLPVYEQEQPGVLYNTAAVVDSDGRYLGKYRKHHIPHLDRFWEKFYFRPGNLGYPVFDTAVGKIGVYICYDRHFPEG
WRNYGLAGAELVFNPSATKPGLSNRLWELEQPAAAAANQYFVGANNRIGAESGEFGDQAVSFYGSSYFVDPRGNYVGEIG
SATDEEIVIRDLDLSLVRQVRDDWQFYRDRRPDTYGPIIAP

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI7706509, Length=281, Percent_Identity=40.9252669039146, Blast_Score=184, Evalue=7e-47,
Organism=Homo sapiens, GI9910460, Length=284, Percent_Identity=32.0422535211268, Blast_Score=134, Evalue=7e-32,
Organism=Homo sapiens, GI297632350, Length=287, Percent_Identity=31.3588850174216, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI297632348, Length=287, Percent_Identity=31.3588850174216, Blast_Score=104, Evalue=9e-23,
Organism=Homo sapiens, GI5031947, Length=282, Percent_Identity=31.5602836879433, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI297632346, Length=183, Percent_Identity=32.7868852459016, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17533173, Length=281, Percent_Identity=39.8576512455516, Blast_Score=185, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17556280, Length=278, Percent_Identity=28.0575539568345, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323383, Length=289, Percent_Identity=29.0657439446367, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322335, Length=300, Percent_Identity=23.3333333333333, Blast_Score=72, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21358471, Length=284, Percent_Identity=39.4366197183099, Blast_Score=191, Evalue=4e-49,
Organism=Drosophila melanogaster, GI21355835, Length=286, Percent_Identity=30.7692307692308, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI17933642, Length=278, Percent_Identity=26.2589928057554, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 31963; Mature: 31832

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIRAALTQATWTGDKESMLAKHERFVAQAAARGAQVICFQELFYGPYFGIVQDKKYYG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCEEECCCCCC
YAEPVPGPVTERFAKLAREHRMVLVLPVYEQEQPGVLYNTAAVVDSDGRYLGKYRKHHIP
CCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEEEEEECCCHHHHHHHHHCCC
HLDRFWEKFYFRPGNLGYPVFDTAVGKIGVYICYDRHFPEGWRNYGLAGAELVFNPSATK
HHHHHHHHHHCCCCCCCCCHHHHHHHHEEEEEEECCCCCHHHHHCCCCCCEEEECCCCCC
PGLSNRLWELEQPAAAAANQYFVGANNRIGAESGEFGDQAVSFYGSSYFVDPRGNYVGEI
CCCCCCCCCCCCCHHHHCCEEEECCCCCCCCCCCCCCHHHHHHCCCEEEECCCCCEEECC
GSATDEEIVIRDLDLSLVRQVRDDWQFYRDRRPDTYGPIIAP
CCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TVIRAALTQATWTGDKESMLAKHERFVAQAAARGAQVICFQELFYGPYFGIVQDKKYYG
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCCCEEECCCCCC
YAEPVPGPVTERFAKLAREHRMVLVLPVYEQEQPGVLYNTAAVVDSDGRYLGKYRKHHIP
CCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCEEEEEEEEEECCCHHHHHHHHHCCC
HLDRFWEKFYFRPGNLGYPVFDTAVGKIGVYICYDRHFPEGWRNYGLAGAELVFNPSATK
HHHHHHHHHHCCCCCCCCCHHHHHHHHEEEEEEECCCCCHHHHHCCCCCCEEEECCCCCC
PGLSNRLWELEQPAAAAANQYFVGANNRIGAESGEFGDQAVSFYGSSYFVDPRGNYVGEI
CCCCCCCCCCCCCHHHHCCEEEECCCCCCCCCCCCCCHHHHHHCCCEEEECCCCCEEECC
GSATDEEIVIRDLDLSLVRQVRDDWQFYRDRRPDTYGPIIAP
CCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]