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The map label for this gene is braE [H]

Identifier: 169629709

GI number: 169629709

Start: 2664410

End: 2665582

Strand: Reverse

Name: braE [H]

Synonym: MAB_2624c

Alternate gene names: 169629709

Gene position: 2665582-2664410 (Counterclockwise)

Preceding gene: 169629710

Following gene: 169629708

Centisome position: 52.6

GC content: 62.15

Gene sequence:

>1173_bases
ATGAGCGCAGCACGTGCCCTGGCACCCGGCGACTCCATCCGACGGTGGTGGTCCGAGCTGGCCCGTCCGGTGCAGTGGGT
GTACGGGGTAGTCGGATTCGCATTGCTTGCGCTGCTGCCGGTCTGGAAGATCCCCTATCTAGATACCCCCAACACCAGCT
TCGGCGGGGTGATGGCCCAGTTTGCGATGGTCGCGCTCATCGCGATAGGCCTCAACGTCGTTGTGGGACAAGCGGGATTG
CTCGACCTAGGCTATGTCGGTTTCTATGCGATTGGTGCCTACACAGTCGCCTTGCTCACCAGTCCCGACAGCCCGTGGAA
TCGGATGGGCACGTCGGCGTTTTTCAGTTCGGACTGGGCATGGCTGTCCTGCCTGCCGATCGCCGTGGCCATCACTGCGT
GCTTCGGCCTGATCCTCGGTACGCCCACGCTGCGGCTTCGGGGCGACTACCTGGCTATCGTCACGCTCGGTTTCGGCGAA
ATCATCCGGCTGCTCGCAGATAACCTGGATGTCACCAATGGCCCACGTGGACTGCACAAGGTCGCGTACCCGCATGTGGG
GGAGCAGCACGTGCACGGCGGTGGCGTTTTCTCCAGTGCCAATTCGGGCGGCCATCTGAACTACGGCGTGTGGTGGTACT
GGCTGGGGCTGTGCCTGATTGCCGCGGTGTTGGTGCTGATGGGCAACCTCGAGCGCAGCCGGGTGGGGCGTTCGTGGGTG
GCTATTCGTGAGGACGAGGACGCCGCCGAAATGATGGGCGTGCCCACCTTCCGCTTCAAGCTGTGGGCCTTCGTGATTGG
GGCGAGTATCGGCGGGTTGTCCGGCGCACTGTATGCGGGCCAGGTCCAATATGTGGCATCGCCGACCTTCAACATCATCA
ACTCGATGCTGTTTCTGTGCGCGGTGGTCCTCGGCGGACAAGGCAACAAGCTTGGTGTCGTCGTCGGTGCGTTCATCATC
GTCTACCTGCCAAACCGCCTGTTGGGCGTGCAGGTACAGGGAATCGACCTGGGCAACCTGAAGTATCTGTTCTTCGGGCT
GGCGCTGGTGGTGCTGATGCTCTTCCGCCCACAGGGGCTGTTCCCCGCGCATCAGAAATTGATGGCCTACTGGCGCAAGA
CATCCGAACGTTTGAGTGCCCAGAAACCCGACGAAGTCGAGGCCGGGGCATGA

Upstream 100 bases:

>100_bases
GCACGCAGTGGCGCGATGTGGTGGCGTTCGTACTGCTGGTGTTGGTGCTCATGTTCCGTCCCACCGGCATTCTCGGCGAG
AGCCTAGGGCGGGCAAAGGC

Downstream 100 bases:

>100_bases
CCAAACATCGCGACAGCAGCCTTGATGACGAGCAGGACTCCGTCGAGATGCTCCAGGCCGAGATCGAGGAGGGATCGGAT
GAGGGTGAATCCGGGGTACC

Product: high-affinity branched-chain amino acid ABC transporter (LivM)

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSAARALAPGDSIRRWWSELARPVQWVYGVVGFALLALLPVWKIPYLDTPNTSFGGVMAQFAMVALIAIGLNVVVGQAGL
LDLGYVGFYAIGAYTVALLTSPDSPWNRMGTSAFFSSDWAWLSCLPIAVAITACFGLILGTPTLRLRGDYLAIVTLGFGE
IIRLLADNLDVTNGPRGLHKVAYPHVGEQHVHGGGVFSSANSGGHLNYGVWWYWLGLCLIAAVLVLMGNLERSRVGRSWV
AIREDEDAAEMMGVPTFRFKLWAFVIGASIGGLSGALYAGQVQYVASPTFNIINSMLFLCAVVLGGQGNKLGVVVGAFII
VYLPNRLLGVQVQGIDLGNLKYLFFGLALVVLMLFRPQGLFPAHQKLMAYWRKTSERLSAQKPDEVEAGA

Sequences:

>Translated_390_residues
MSAARALAPGDSIRRWWSELARPVQWVYGVVGFALLALLPVWKIPYLDTPNTSFGGVMAQFAMVALIAIGLNVVVGQAGL
LDLGYVGFYAIGAYTVALLTSPDSPWNRMGTSAFFSSDWAWLSCLPIAVAITACFGLILGTPTLRLRGDYLAIVTLGFGE
IIRLLADNLDVTNGPRGLHKVAYPHVGEQHVHGGGVFSSANSGGHLNYGVWWYWLGLCLIAAVLVLMGNLERSRVGRSWV
AIREDEDAAEMMGVPTFRFKLWAFVIGASIGGLSGALYAGQVQYVASPTFNIINSMLFLCAVVLGGQGNKLGVVVGAFII
VYLPNRLLGVQVQGIDLGNLKYLFFGLALVVLMLFRPQGLFPAHQKLMAYWRKTSERLSAQKPDEVEAGA
>Mature_389_residues
SAARALAPGDSIRRWWSELARPVQWVYGVVGFALLALLPVWKIPYLDTPNTSFGGVMAQFAMVALIAIGLNVVVGQAGLL
DLGYVGFYAIGAYTVALLTSPDSPWNRMGTSAFFSSDWAWLSCLPIAVAITACFGLILGTPTLRLRGDYLAIVTLGFGEI
IRLLADNLDVTNGPRGLHKVAYPHVGEQHVHGGGVFSSANSGGHLNYGVWWYWLGLCLIAAVLVLMGNLERSRVGRSWVA
IREDEDAAEMMGVPTFRFKLWAFVIGASIGGLSGALYAGQVQYVASPTFNIINSMLFLCAVVLGGQGNKLGVVVGAFIIV
YLPNRLLGVQVQGIDLGNLKYLFFGLALVVLMLFRPQGLFPAHQKLMAYWRKTSERLSAQKPDEVEAGA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=319, Percent_Identity=38.871473354232, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 42128; Mature: 41997

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAARALAPGDSIRRWWSELARPVQWVYGVVGFALLALLPVWKIPYLDTPNTSFGGVMAQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
FAMVALIAIGLNVVVGQAGLLDLGYVGFYAIGAYTVALLTSPDSPWNRMGTSAFFSSDWA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHCCCHHHHCCCCH
WLSCLPIAVAITACFGLILGTPTLRLRGDYLAIVTLGFGEIIRLLADNLDVTNGPRGLHK
HHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCHHHH
VAYPHVGEQHVHGGGVFSSANSGGHLNYGVWWYWLGLCLIAAVLVLMGNLERSRVGRSWV
HCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEE
AIREDEDAAEMMGVPTFRFKLWAFVIGASIGGLSGALYAGQVQYVASPTFNIINSMLFLC
EEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHCEEEEECCCHHHHHHHHHHHH
AVVLGGQGNKLGVVVGAFIIVYLPNRLLGVQVQGIDLGNLKYLFFGLALVVLMLFRPQGL
HHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
FPAHQKLMAYWRKTSERLSAQKPDEVEAGA
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SAARALAPGDSIRRWWSELARPVQWVYGVVGFALLALLPVWKIPYLDTPNTSFGGVMAQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
FAMVALIAIGLNVVVGQAGLLDLGYVGFYAIGAYTVALLTSPDSPWNRMGTSAFFSSDWA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEECCCCCHHHCCCHHHHCCCCH
WLSCLPIAVAITACFGLILGTPTLRLRGDYLAIVTLGFGEIIRLLADNLDVTNGPRGLHK
HHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCHHHH
VAYPHVGEQHVHGGGVFSSANSGGHLNYGVWWYWLGLCLIAAVLVLMGNLERSRVGRSWV
HCCCCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEE
AIREDEDAAEMMGVPTFRFKLWAFVIGASIGGLSGALYAGQVQYVASPTFNIINSMLFLC
EEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHCEEEEECCCHHHHHHHHHHHH
AVVLGGQGNKLGVVVGAFIIVYLPNRLLGVQVQGIDLGNLKYLFFGLALVVLMLFRPQGL
HHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCC
FPAHQKLMAYWRKTSERLSAQKPDEVEAGA
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]