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The map label for this gene is sigF [H]

Identifier: 169629597

GI number: 169629597

Start: 2565465

End: 2566283

Strand: Direct

Name: sigF [H]

Synonym: MAB_2511

Alternate gene names: 169629597

Gene position: 2565465-2566283 (Clockwise)

Preceding gene: 169629594

Following gene: 169629598

Centisome position: 50.63

GC content: 59.34

Gene sequence:

>819_bases
ATGGAATGCCCTGTTGATGTACCGGAGACCGGACACTCACAGGTGAAACCACGCGTTGGCCGGCCGACTGACGACTACGC
GGACGTACCGGACATGTTTCGGTCTCTCGCCGGCTTGGCGGAGCACAGTGACGCATACCAACGTAAGCACGAGGAGATAG
TCACCAGATGCCTGCCGCTGGCCGACAATGTCGCACGTCATTTCGATCGCCGTGGTGAAGATCTCGAGGACCTGATCCAG
GTTGCCCGAGTGGGCTTGCTCAATGCGGTCAACCGTTTTGACCCGGAAAAGGGCGCCAGCTTCCTCGGCTTTGCGGTTCC
TACCATGATGGGGGAGGTGCGCCGGCACTTTCGCGACCACGGATGGTCGCTGCACGTACCGCGGGCTATAAGAGATAGAC
ACGCTCAAATCTCCAGGGCCACTGCGGAATTGACCCAGGAGCTACAGCGGGCGCCTACCGCCGGGGAGTTGGCGGCAGAG
TTGGGTATCGACCGCGAAGAGGTGGTGGAATCTCTGATTGCCGCGGACGCCTATCAGCCTCAATCCATCGAGGCGCCCCT
CAGCCACGGCGATCAGGAATCCAAGCATCTTTCCGATCTGCTGGGGAATGAAGATCCAGCCCTGGAACACGTGACCAACC
GCGAGGCAGTACGGCCTCTTTTAGCCAATTTACCCCTGCGAGAGCGAACCGTGCTTGAGCTCCGGTTTTTCAAGGGAATG
ACCCAGAGCCAGATTGCCGCACAGATTGGTGTTTCGCAGATGCACGTATCCCGGATTCTCAGCGACACCTTGCGGTACCT
GCGAGAACAGATGGCGTAG

Upstream 100 bases:

>100_bases
GGCGGCGGACCACCGTCGGATGTAGGCGCAGCTGGCCGACATGGACGTTGCCGGCCAAATTATATGAGCTGCTCCGTCGA
CAGCGTTGAGAGGTTTACAC

Downstream 100 bases:

>100_bases
ACCGCCCTGTCCGCGGTTAGAGAACTGCTTGACCGGGTATATGACGTGCCAAGCGGTCTGCCCTGGAGGTGTGCAATGAG
CCCATTGTCCTTGATTCGAC

Product: RNA polymerase sigma factor SigF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MECPVDVPETGHSQVKPRVGRPTDDYADVPDMFRSLAGLAEHSDAYQRKHEEIVTRCLPLADNVARHFDRRGEDLEDLIQ
VARVGLLNAVNRFDPEKGASFLGFAVPTMMGEVRRHFRDHGWSLHVPRAIRDRHAQISRATAELTQELQRAPTAGELAAE
LGIDREEVVESLIAADAYQPQSIEAPLSHGDQESKHLSDLLGNEDPALEHVTNREAVRPLLANLPLRERTVLELRFFKGM
TQSQIAAQIGVSQMHVSRILSDTLRYLREQMA

Sequences:

>Translated_272_residues
MECPVDVPETGHSQVKPRVGRPTDDYADVPDMFRSLAGLAEHSDAYQRKHEEIVTRCLPLADNVARHFDRRGEDLEDLIQ
VARVGLLNAVNRFDPEKGASFLGFAVPTMMGEVRRHFRDHGWSLHVPRAIRDRHAQISRATAELTQELQRAPTAGELAAE
LGIDREEVVESLIAADAYQPQSIEAPLSHGDQESKHLSDLLGNEDPALEHVTNREAVRPLLANLPLRERTVLELRFFKGM
TQSQIAAQIGVSQMHVSRILSDTLRYLREQMA
>Mature_272_residues
MECPVDVPETGHSQVKPRVGRPTDDYADVPDMFRSLAGLAEHSDAYQRKHEEIVTRCLPLADNVARHFDRRGEDLEDLIQ
VARVGLLNAVNRFDPEKGASFLGFAVPTMMGEVRRHFRDHGWSLHVPRAIRDRHAQISRATAELTQELQRAPTAGELAAE
LGIDREEVVESLIAADAYQPQSIEAPLSHGDQESKHLSDLLGNEDPALEHVTNREAVRPLLANLPLRERTVLELRFFKGM
TQSQIAAQIGVSQMHVSRILSDTLRYLREQMA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for normal spore maturation [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. SigB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=233, Percent_Identity=29.1845493562232, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=31.1881188118812, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789098, Length=226, Percent_Identity=28.7610619469027, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 30544; Mature: 30544

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MECPVDVPETGHSQVKPRVGRPTDDYADVPDMFRSLAGLAEHSDAYQRKHEEIVTRCLPL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADNVARHFDRRGEDLEDLIQVARVGLLNAVNRFDPEKGASFLGFAVPTMMGEVRRHFRDH
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHC
GWSLHVPRAIRDRHAQISRATAELTQELQRAPTAGELAAELGIDREEVVESLIAADAYQP
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCC
QSIEAPLSHGDQESKHLSDLLGNEDPALEHVTNREAVRPLLANLPLRERTVLELRFFKGM
CCCCCCHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
TQSQIAAQIGVSQMHVSRILSDTLRYLREQMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MECPVDVPETGHSQVKPRVGRPTDDYADVPDMFRSLAGLAEHSDAYQRKHEEIVTRCLPL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADNVARHFDRRGEDLEDLIQVARVGLLNAVNRFDPEKGASFLGFAVPTMMGEVRRHFRDH
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHC
GWSLHVPRAIRDRHAQISRATAELTQELQRAPTAGELAAELGIDREEVVESLIAADAYQP
CCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCC
QSIEAPLSHGDQESKHLSDLLGNEDPALEHVTNREAVRPLLANLPLRERTVLELRFFKGM
CCCCCCHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
TQSQIAAQIGVSQMHVSRILSDTLRYLREQMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7476207; 12000953 [H]