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The map label for this gene is argF [H]

Identifier: 169629426

GI number: 169629426

Start: 2395350

End: 2396291

Strand: Direct

Name: argF [H]

Synonym: MAB_2340

Alternate gene names: 169629426

Gene position: 2395350-2396291 (Clockwise)

Preceding gene: 169629425

Following gene: 169629427

Centisome position: 47.27

GC content: 64.12

Gene sequence:

>942_bases
ATGATTCAGTCGAAGTCACTTCGCCATTTTCTGAGGGACGACGACCTCTCACCTGCCGAACAGGCCGAAGTCCTCACGTT
GGCAGCCGAACTCAAGAAGGCGCCGTTCTCGCGGCGCCCGCTGGAGGGGCCGCATGGGGTAGCCGTCATCTTCGAGAAAA
ACTCCACGCGAACCCGATTCTCGTTCGAAATGGGTATCGCGCAGTTGGGCGGGCACGCGGTGGTCGTCGATAGCCGCACG
ACTCAGCTCGGGCGCGAAGAGACCCTGGAGGACACCGGGCAGGTGCTGTCGCGCTACGTGGAAGCCATCGTGTGGCGTAC
CTACGCGCAGGAGCGCTTGACCGCCATGGCAAGTGGTGCCTCCGTACCCATTGTCAATGCCCTCTCCGACGATTTTCATC
CGTGCCAGGTACTCGCTGATCTGCAGACGCTGGCCGAGCGCAAGGGCTCGCTGGATGGGCTTCGTCTGACGTACCTGGGT
GATGGCGCCAACAATATGGCCCACTCGTTGATGCTCGGTGGGGTGACAGCCGGTATCCACGTGACCATCGCCGCGCCCAA
CGGGTTTGCGCCACACGCCAAATACGTGGCCGAGGCCGAGAAGGTGGCCGCGACTACGGGTGCTTCGGTGTCGGTCACCG
CCGACCCGCGCTCCGCCGTGGCCAGGGCCGATGTGCTGGTCACCGATGCCTGGACCTCGATGGGGCAGGAGAACGATGGG
CTCGACAGGGTACGGCCGTTCCGGGCGTTCCAGGTGAACGAGGACCTGCTTGCCCTGGCGGATTCCGAAGCCGTTGTCCT
GCACTGCCTTCCCGCTCACCGCGGTCATGAAATCACGGACGGGGTGATAGACGGCCCGCGCAGCGCGGTGTGGGACGAGG
CCGAAAACCGTCTGCACGCCCAGAAGGCGCTGCTGGTGTGGTTACTGGAGAACCGACGATGA

Upstream 100 bases:

>100_bases
TGCGGCTGGCGCCACCGCTGATCATCACCGATGAACAAATCGGGGAATTTGTCACCGCGCTGCCGGGAATCCTCGACACC
GCCGCAGAACAGGAGCAGGC

Downstream 100 bases:

>100_bases
CTGCCTCGCACGCGGCCGATACCCGAGCCGGGCGGCAAGCGCGCATCATCGCGCTGCTGTCCACCCAGTCGGTGCGCAGC
CAGAGCGAATTGGCCGCGTT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MIQSKSLRHFLRDDDLSPAEQAEVLTLAAELKKAPFSRRPLEGPHGVAVIFEKNSTRTRFSFEMGIAQLGGHAVVVDSRT
TQLGREETLEDTGQVLSRYVEAIVWRTYAQERLTAMASGASVPIVNALSDDFHPCQVLADLQTLAERKGSLDGLRLTYLG
DGANNMAHSLMLGGVTAGIHVTIAAPNGFAPHAKYVAEAEKVAATTGASVSVTADPRSAVARADVLVTDAWTSMGQENDG
LDRVRPFRAFQVNEDLLALADSEAVVLHCLPAHRGHEITDGVIDGPRSAVWDEAENRLHAQKALLVWLLENRR

Sequences:

>Translated_313_residues
MIQSKSLRHFLRDDDLSPAEQAEVLTLAAELKKAPFSRRPLEGPHGVAVIFEKNSTRTRFSFEMGIAQLGGHAVVVDSRT
TQLGREETLEDTGQVLSRYVEAIVWRTYAQERLTAMASGASVPIVNALSDDFHPCQVLADLQTLAERKGSLDGLRLTYLG
DGANNMAHSLMLGGVTAGIHVTIAAPNGFAPHAKYVAEAEKVAATTGASVSVTADPRSAVARADVLVTDAWTSMGQENDG
LDRVRPFRAFQVNEDLLALADSEAVVLHCLPAHRGHEITDGVIDGPRSAVWDEAENRLHAQKALLVWLLENRR
>Mature_313_residues
MIQSKSLRHFLRDDDLSPAEQAEVLTLAAELKKAPFSRRPLEGPHGVAVIFEKNSTRTRFSFEMGIAQLGGHAVVVDSRT
TQLGREETLEDTGQVLSRYVEAIVWRTYAQERLTAMASGASVPIVNALSDDFHPCQVLADLQTLAERKGSLDGLRLTYLG
DGANNMAHSLMLGGVTAGIHVTIAAPNGFAPHAKYVAEAEKVAATTGASVSVTADPRSAVARADVLVTDAWTSMGQENDG
LDRVRPFRAFQVNEDLLALADSEAVVLHCLPAHRGHEITDGVIDGPRSAVWDEAENRLHAQKALLVWLLENRR

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=305, Percent_Identity=40.9836065573771, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI18105007, Length=316, Percent_Identity=25.9493670886076, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1790703, Length=324, Percent_Identity=36.4197530864198, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1786469, Length=323, Percent_Identity=35.6037151702786, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI2367364, Length=313, Percent_Identity=33.5463258785943, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI48994908, Length=353, Percent_Identity=27.1954674220963, Blast_Score=86, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322373, Length=325, Percent_Identity=34.7692307692308, Blast_Score=190, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6322331, Length=270, Percent_Identity=27.4074074074074, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24642586, Length=315, Percent_Identity=26.984126984127, Blast_Score=91, Evalue=7e-19,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 33933; Mature: 33933

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSKSLRHFLRDDDLSPAEQAEVLTLAAELKKAPFSRRPLEGPHGVAVIFEKNSTRTRF
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEE
SFEMGIAQLGGHAVVVDSRTTQLGREETLEDTGQVLSRYVEAIVWRTYAQERLTAMASGA
EEEHHHHHHCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SVPIVNALSDDFHPCQVLADLQTLAERKGSLDGLRLTYLGDGANNMAHSLMLGGVTAGIH
CCCEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCEEECEE
VTIAAPNGFAPHAKYVAEAEKVAATTGASVSVTADPRSAVARADVLVTDAWTSMGQENDG
EEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHEEEEHHHHHCCCCCCC
LDRVRPFRAFQVNEDLLALADSEAVVLHCLPAHRGHEITDGVIDGPRSAVWDEAENRLHA
HHHHCCCCEEECCCHHEEECCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QKALLVWLLENRR
HHHHHEEEECCCC
>Mature Secondary Structure
MIQSKSLRHFLRDDDLSPAEQAEVLTLAAELKKAPFSRRPLEGPHGVAVIFEKNSTRTRF
CCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEE
SFEMGIAQLGGHAVVVDSRTTQLGREETLEDTGQVLSRYVEAIVWRTYAQERLTAMASGA
EEEHHHHHHCCEEEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SVPIVNALSDDFHPCQVLADLQTLAERKGSLDGLRLTYLGDGANNMAHSLMLGGVTAGIH
CCCEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCEEECEE
VTIAAPNGFAPHAKYVAEAEKVAATTGASVSVTADPRSAVARADVLVTDAWTSMGQENDG
EEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHEEEEHHHHHCCCCCCC
LDRVRPFRAFQVNEDLLALADSEAVVLHCLPAHRGHEITDGVIDGPRSAVWDEAENRLHA
HHHHCCCCEEECCCHHEEECCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QKALLVWLLENRR
HHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA