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The map label for this gene is baiB [H]

Identifier: 169629402

GI number: 169629402

Start: 2369798

End: 2371288

Strand: Direct

Name: baiB [H]

Synonym: MAB_2316

Alternate gene names: 169629402

Gene position: 2369798-2371288 (Clockwise)

Preceding gene: 169629401

Following gene: 169629407

Centisome position: 46.77

GC content: 64.12

Gene sequence:

>1491_bases
GTGGCAGTCATGACTGACGCCGCCGTTGGTGAACCGTTCGTTCGCATCCTCGCCAACTTGGCAGATGCAGAACCGGACGC
CCCGGCTGTCACATGCGGTACCGATTCGGTTACCCGGGCAGAACTGGAGCGGCGTGCCACCGCGTTGGCGCATGCGTACC
AGCGTCTCGGTGTGCGACAGGGTGACCTCGTCACCATCGGGCTGCCCAACTCGGTGGAGTTCTTCGCTGCCCAGATCGCG
GTGTGGAAACTGGGTGCGACGCCGCAGCCGGTGTCCTGGCGGCTGCCCCTTGCCGAACGCCGCGCCATCGTCGAACTGGC
CGACTCGACGCTCGTGGTCGGGGTAGACCCGGCCGACCATCCTGATCGGGTTTGTGTATCAGCGGGATTCGAGCCCGGTG
CCGAGGACTACCTTGCCGGTCCCGTGCCCGAGGTGGTATCTCCGGCCTGGAAGGCGCCCACCTCCGGTGGCAGCACCGGC
CGGCCCAAGATCATCGTCGCGCCTACCCCCGCGGTCATCACCGGAACGGCAGCCGGCGAGATTATGGGTATGCAGCACGC
CGACGCCCAACTGGTCGCCGGCCCGCTGTATCACAATGCCCCGCTGATGTTTTCGTCATATGGCCTGCTCCTTGGACATC
ATCTGGTGGTGCAACCGAAGTTCGACGCACTGGAGGCGCTTGAGCTGATCGGTACCCACAAGGTCACCTGGCTGCAAGTG
GTACCCACCATGATGTCGCGCATGCTGCGTGAACTCCGTCAGCATCCCGGACGGTTCGATCTGTCTACTATTCGGGTCCT
GTGGCATATGGCGGCTCCCTGCCCGGTGTGGTTGAAGCAAGCGTGGATTGACCTGCTGGGCCCCGAGGTCATCTGGGAGC
TGTATGCCGGCACCGAGGCCATCGGCGGAACCATCATCACCGGACAGGAATGGCTGGCGCACCGTGGGTCCGTCGGTAAG
CCGGCACTCGGTGAACTCGCCATCCTCGACGAGAACGGAAACCCGCTTCCCGCGGGCGAGGTGGGGGAGATCTTCATGCG
CCCGTGGGAAGGAATGCCCACCCCGTACAAGTACCTGGGTGCTGAGATCAAGCGGTTCGGCACCTGGGAATCGATCGGCG
ATCTCGGCTGGCTGGACGATGACGGCTACCTGTACATGAGTGATCGCCGTACCGACTTGATCGTCACGGGCGGGGCGAAT
GTGTTTCCCGCGGAAGTGGAATCGGTGATGAACGGCTACCCGGGTGTGATCGACAGCGTTGTCGTGGGTCTACCCGATGA
TGACCTCGGGCAGAGCGTGCATGCCGTTGTACACGCCGAACCCAACGTGACCGAGGATCAGTTGCGCGACTATCTGCTGG
ACCAGATTGTCCGGTACAAGGTTCCGCGCACCATCGAGTTTGTCGCCGACCCGCTGCGGGAGGACTCGGGCAAGGTGCGG
CGCAGCCTCATCCGTGAGCAGGCGACTGCCCGTCGGGCAGCCGCGGGTTAG

Upstream 100 bases:

>100_bases
CCCGTGCGAAACGGCAAGGCGCCTGCGAAGGCGCCCGCCAAGAGTACCGGGAAGACACTGGCCAAAGCGCGGACCAGGAA
GGTTGTCACTCCGGTCTAGG

Downstream 100 bases:

>100_bases
TCCTCGCGCATAGACTCACGGATTTCCGCAAGCCGCTGTGCGGCCGCCCTGTTTTTCGCTTCGAACTGTGCCTCGACAGC
GCGGCCTTCTGCACTCTCGG

Product: acid-CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MAVMTDAAVGEPFVRILANLADAEPDAPAVTCGTDSVTRAELERRATALAHAYQRLGVRQGDLVTIGLPNSVEFFAAQIA
VWKLGATPQPVSWRLPLAERRAIVELADSTLVVGVDPADHPDRVCVSAGFEPGAEDYLAGPVPEVVSPAWKAPTSGGSTG
RPKIIVAPTPAVITGTAAGEIMGMQHADAQLVAGPLYHNAPLMFSSYGLLLGHHLVVQPKFDALEALELIGTHKVTWLQV
VPTMMSRMLRELRQHPGRFDLSTIRVLWHMAAPCPVWLKQAWIDLLGPEVIWELYAGTEAIGGTIITGQEWLAHRGSVGK
PALGELAILDENGNPLPAGEVGEIFMRPWEGMPTPYKYLGAEIKRFGTWESIGDLGWLDDDGYLYMSDRRTDLIVTGGAN
VFPAEVESVMNGYPGVIDSVVVGLPDDDLGQSVHAVVHAEPNVTEDQLRDYLLDQIVRYKVPRTIEFVADPLREDSGKVR
RSLIREQATARRAAAG

Sequences:

>Translated_496_residues
MAVMTDAAVGEPFVRILANLADAEPDAPAVTCGTDSVTRAELERRATALAHAYQRLGVRQGDLVTIGLPNSVEFFAAQIA
VWKLGATPQPVSWRLPLAERRAIVELADSTLVVGVDPADHPDRVCVSAGFEPGAEDYLAGPVPEVVSPAWKAPTSGGSTG
RPKIIVAPTPAVITGTAAGEIMGMQHADAQLVAGPLYHNAPLMFSSYGLLLGHHLVVQPKFDALEALELIGTHKVTWLQV
VPTMMSRMLRELRQHPGRFDLSTIRVLWHMAAPCPVWLKQAWIDLLGPEVIWELYAGTEAIGGTIITGQEWLAHRGSVGK
PALGELAILDENGNPLPAGEVGEIFMRPWEGMPTPYKYLGAEIKRFGTWESIGDLGWLDDDGYLYMSDRRTDLIVTGGAN
VFPAEVESVMNGYPGVIDSVVVGLPDDDLGQSVHAVVHAEPNVTEDQLRDYLLDQIVRYKVPRTIEFVADPLREDSGKVR
RSLIREQATARRAAAG
>Mature_495_residues
AVMTDAAVGEPFVRILANLADAEPDAPAVTCGTDSVTRAELERRATALAHAYQRLGVRQGDLVTIGLPNSVEFFAAQIAV
WKLGATPQPVSWRLPLAERRAIVELADSTLVVGVDPADHPDRVCVSAGFEPGAEDYLAGPVPEVVSPAWKAPTSGGSTGR
PKIIVAPTPAVITGTAAGEIMGMQHADAQLVAGPLYHNAPLMFSSYGLLLGHHLVVQPKFDALEALELIGTHKVTWLQVV
PTMMSRMLRELRQHPGRFDLSTIRVLWHMAAPCPVWLKQAWIDLLGPEVIWELYAGTEAIGGTIITGQEWLAHRGSVGKP
ALGELAILDENGNPLPAGEVGEIFMRPWEGMPTPYKYLGAEIKRFGTWESIGDLGWLDDDGYLYMSDRRTDLIVTGGANV
FPAEVESVMNGYPGVIDSVVVGLPDDDLGQSVHAVVHAEPNVTEDQLRDYLLDQIVRYKVPRTIEFVADPLREDSGKVRR
SLIREQATARRAAAG

Specific function: Active with a large number of C-24 bile acids possessing unhindered C-24 carboxyl groups. Requires ATP and produces AMP and pyrophosphate in addition to the bile-acid CoA thioester [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI122937307, Length=263, Percent_Identity=31.5589353612167, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI42544132, Length=299, Percent_Identity=28.0936454849498, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI156151445, Length=353, Percent_Identity=25.4957507082153, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI38505220, Length=478, Percent_Identity=22.5941422594142, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI58082049, Length=301, Percent_Identity=26.578073089701, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI157311624, Length=300, Percent_Identity=26.3333333333333, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI157311622, Length=300, Percent_Identity=26.3333333333333, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI115511026, Length=185, Percent_Identity=30.8108108108108, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI187761345, Length=521, Percent_Identity=25.5278310940499, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI187761343, Length=521, Percent_Identity=25.5278310940499, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI13375727, Length=534, Percent_Identity=22.8464419475655, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI28416953, Length=524, Percent_Identity=23.6641221374046, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788107, Length=525, Percent_Identity=25.1428571428571, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI221142682, Length=523, Percent_Identity=22.9445506692161, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786810, Length=516, Percent_Identity=25, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI145693145, Length=519, Percent_Identity=23.6994219653179, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786801, Length=438, Percent_Identity=24.6575342465753, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790505, Length=539, Percent_Identity=23.0055658627087, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=488, Percent_Identity=28.8934426229508, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=490, Percent_Identity=27.7551020408163, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=355, Percent_Identity=27.6056338028169, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=355, Percent_Identity=27.6056338028169, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=355, Percent_Identity=27.6056338028169, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17559526, Length=365, Percent_Identity=27.3972602739726, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=519, Percent_Identity=25.8188824662813, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=518, Percent_Identity=25.6756756756757, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=354, Percent_Identity=28.2485875706215, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=540, Percent_Identity=22.5925925925926, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=348, Percent_Identity=26.1494252873563, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323182, Length=555, Percent_Identity=25.5855855855856, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=526, Percent_Identity=23.0038022813688, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355181, Length=494, Percent_Identity=25.9109311740891, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922652, Length=458, Percent_Identity=24.235807860262, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648260, Length=355, Percent_Identity=26.7605633802817, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356441, Length=511, Percent_Identity=23.679060665362, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24581924, Length=368, Percent_Identity=25, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648253, Length=486, Percent_Identity=23.2510288065844, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24648255, Length=486, Percent_Identity=23.2510288065844, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24656500, Length=492, Percent_Identity=22.1544715447154, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356947, Length=482, Percent_Identity=23.2365145228216, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI18859661, Length=511, Percent_Identity=22.5048923679061, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI62472339, Length=544, Percent_Identity=22.6102941176471, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21358303, Length=248, Percent_Identity=26.6129032258064, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24667955, Length=544, Percent_Identity=22.6102941176471, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24667959, Length=374, Percent_Identity=25.4010695187166, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI20130357, Length=283, Percent_Identity=26.5017667844523, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24653035, Length=152, Percent_Identity=32.8947368421053, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648257, Length=288, Percent_Identity=24.3055555555556, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI161076582, Length=220, Percent_Identity=25.9090909090909, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 53694; Mature: 53563

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVMTDAAVGEPFVRILANLADAEPDAPAVTCGTDSVTRAELERRATALAHAYQRLGVRQ
CCEECCCCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
GDLVTIGLPNSVEFFAAQIAVWKLGATPQPVSWRLPLAERRAIVELADSTLVVGVDPADH
CCEEEEECCCCHHHHHHHHHEEECCCCCCCCEEECCHHHHHHHHEECCCEEEEECCCCCC
PDRVCVSAGFEPGAEDYLAGPVPEVVSPAWKAPTSGGSTGRPKIIVAPTPAVITGTAAGE
CCCEEEECCCCCCCHHHCCCCCHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCHH
IMGMQHADAQLVAGPLYHNAPLMFSSYGLLLGHHLVVQPKFDALEALELIGTHKVTWLQV
HCCCCCCCCHHEECCCCCCCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCHHHHHH
VPTMMSRMLRELRQHPGRFDLSTIRVLWHMAAPCPVWLKQAWIDLLGPEVIWELYAGTEA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHCCHH
IGGTIITGQEWLAHRGSVGKPALGELAILDENGNPLPAGEVGEIFMRPWEGMPTPYKYLG
HCCEEEECHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCHHHHH
AEIKRFGTWESIGDLGWLDDDGYLYMSDRRTDLIVTGGANVFPAEVESVMNGYPGVIDSV
HHHHHCCCHHHHCCCCCCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHCCCCCCHHHH
VVGLPDDDLGQSVHAVVHAEPNVTEDQLRDYLLDQIVRYKVPRTIEFVADPLREDSGKVR
EECCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHH
RSLIREQATARRAAAG
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVMTDAAVGEPFVRILANLADAEPDAPAVTCGTDSVTRAELERRATALAHAYQRLGVRQ
CEECCCCCCCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
GDLVTIGLPNSVEFFAAQIAVWKLGATPQPVSWRLPLAERRAIVELADSTLVVGVDPADH
CCEEEEECCCCHHHHHHHHHEEECCCCCCCCEEECCHHHHHHHHEECCCEEEEECCCCCC
PDRVCVSAGFEPGAEDYLAGPVPEVVSPAWKAPTSGGSTGRPKIIVAPTPAVITGTAAGE
CCCEEEECCCCCCCHHHCCCCCHHHHCCCCCCCCCCCCCCCCEEEEECCCCEEECCCCHH
IMGMQHADAQLVAGPLYHNAPLMFSSYGLLLGHHLVVQPKFDALEALELIGTHKVTWLQV
HCCCCCCCCHHEECCCCCCCCHHHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCHHHHHH
VPTMMSRMLRELRQHPGRFDLSTIRVLWHMAAPCPVWLKQAWIDLLGPEVIWELYAGTEA
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHCCHH
IGGTIITGQEWLAHRGSVGKPALGELAILDENGNPLPAGEVGEIFMRPWEGMPTPYKYLG
HCCEEEECHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCHHHHH
AEIKRFGTWESIGDLGWLDDDGYLYMSDRRTDLIVTGGANVFPAEVESVMNGYPGVIDSV
HHHHHCCCHHHHCCCCCCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHCCCCCCHHHH
VVGLPDDDLGQSVHAVVHAEPNVTEDQLRDYLLDQIVRYKVPRTIEFVADPLREDSGKVR
EECCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHH
RSLIREQATARRAAAG
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254270; 1551828 [H]