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The map label for this gene is menE [H]

Identifier: 169629149

GI number: 169629149

Start: 2060888

End: 2061262

Strand: Reverse

Name: menE [H]

Synonym: MAB_2062c

Alternate gene names: 169629149

Gene position: 2061262-2060888 (Counterclockwise)

Preceding gene: 169629150

Following gene: 169629148

Centisome position: 40.68

GC content: 67.73

Gene sequence:

>375_bases
ATGGACGCGGAGGGCTTCATCACCCTCGTCGACCGCAAGAAGGACATGATCATCTCCGGCGGCGAGAACGTCTACCCGAT
CGAGGTCGAGCAGGTCCTCTTCCGCCACCCGGCGGTGTTCGACGCCGCCGTGATCGGCGGTAAGGACGCGAAGTGGGGCG
AACGCGTCGTCGCGGTCGTGGTTGCCGACCCGGCGACGCAGGCGCCGAGCGCCGAGGAGATCGTGGCGTGGTGCCGGGAG
CGGCTGGCACACTTCAAGTGTCCGCGTGACGTGCACTTCGTCGCCGAACTGCCGCGCAACGCCACCGGCAAGCTGCTCAA
GACCGAGCTGCGCGCGCAGTTCACGGGCATCGAGGGCGGAGTCGTTTTGCGCTGA

Upstream 100 bases:

>100_bases
GCCGAGAACATCAGCACGCGGGCCGGGTCGATCGGCCGCGTCGCGATGCATGTCGACGGCGTTGCGCGGGGGCTGGTTCC
ACACGGGCGATCTCGGGCGC

Downstream 100 bases:

>100_bases
GTCGTGCCGCTGCACATCGCGGGTTCGGAGCAGGGCCCCTAAATCATGAACTGAGAAAAGGATCGACAGCATCGGCGGGG
GCTAGCGGTTTCGTTCACCT

Product: long-chain-fatty-acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MDAEGFITLVDRKKDMIISGGENVYPIEVEQVLFRHPAVFDAAVIGGKDAKWGERVVAVVVADPATQAPSAEEIVAWCRE
RLAHFKCPRDVHFVAELPRNATGKLLKTELRAQFTGIEGGVVLR

Sequences:

>Translated_124_residues
MDAEGFITLVDRKKDMIISGGENVYPIEVEQVLFRHPAVFDAAVIGGKDAKWGERVVAVVVADPATQAPSAEEIVAWCRE
RLAHFKCPRDVHFVAELPRNATGKLLKTELRAQFTGIEGGVVLR
>Mature_124_residues
MDAEGFITLVDRKKDMIISGGENVYPIEVEQVLFRHPAVFDAAVIGGKDAKWGERVVAVVVADPATQAPSAEEIVAWCRE
RLAHFKCPRDVHFVAELPRNATGKLLKTELRAQFTGIEGGVVLR

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=113, Percent_Identity=40.7079646017699, Blast_Score=97, Evalue=3e-21,
Organism=Homo sapiens, GI115511026, Length=120, Percent_Identity=38.3333333333333, Blast_Score=85, Evalue=1e-17,
Organism=Homo sapiens, GI42544132, Length=117, Percent_Identity=36.7521367521368, Blast_Score=83, Evalue=7e-17,
Organism=Homo sapiens, GI38505220, Length=117, Percent_Identity=36.7521367521368, Blast_Score=80, Evalue=3e-16,
Organism=Homo sapiens, GI122937307, Length=117, Percent_Identity=39.3162393162393, Blast_Score=80, Evalue=5e-16,
Organism=Homo sapiens, GI157311624, Length=114, Percent_Identity=35.0877192982456, Blast_Score=73, Evalue=6e-14,
Organism=Homo sapiens, GI157311622, Length=114, Percent_Identity=35.0877192982456, Blast_Score=73, Evalue=6e-14,
Organism=Homo sapiens, GI187761345, Length=100, Percent_Identity=41, Blast_Score=72, Evalue=8e-14,
Organism=Homo sapiens, GI187761343, Length=100, Percent_Identity=41, Blast_Score=72, Evalue=8e-14,
Organism=Homo sapiens, GI58082049, Length=114, Percent_Identity=35.0877192982456, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788107, Length=117, Percent_Identity=44.4444444444444, Blast_Score=100, Evalue=3e-23,
Organism=Escherichia coli, GI145693145, Length=112, Percent_Identity=41.9642857142857, Blast_Score=92, Evalue=1e-20,
Organism=Escherichia coli, GI221142682, Length=110, Percent_Identity=37.2727272727273, Blast_Score=81, Evalue=2e-17,
Organism=Escherichia coli, GI1786810, Length=113, Percent_Identity=40.7079646017699, Blast_Score=74, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71994703, Length=124, Percent_Identity=44.3548387096774, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=124, Percent_Identity=44.3548387096774, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=124, Percent_Identity=44.3548387096774, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=116, Percent_Identity=40.5172413793103, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560308, Length=114, Percent_Identity=38.5964912280702, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI32563687, Length=121, Percent_Identity=34.7107438016529, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=113, Percent_Identity=34.5132743362832, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17559526, Length=112, Percent_Identity=35.7142857142857, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71985884, Length=114, Percent_Identity=37.719298245614, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=113, Percent_Identity=36.283185840708, Blast_Score=83, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24581924, Length=122, Percent_Identity=40.1639344262295, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18859661, Length=115, Percent_Identity=42.6086956521739, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 13641; Mature: 13641

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAEGFITLVDRKKDMIISGGENVYPIEVEQVLFRHPAVFDAAVIGGKDAKWGERVVAVV
CCCCCEEEEEECCCCEEEECCCCEEEEHHHHHHHHCCCHHHHEEECCCCCCCCCEEEEEE
VADPATQAPSAEEIVAWCRERLAHFKCPRDVHFVAELPRNATGKLLKTELRAQFTGIEGG
EECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHEECCCCCCCCHHHHHHHHHHHCCCCCC
VVLR
EEEC
>Mature Secondary Structure
MDAEGFITLVDRKKDMIISGGENVYPIEVEQVLFRHPAVFDAAVIGGKDAKWGERVVAVV
CCCCCEEEEEECCCCEEEECCCCEEEEHHHHHHHHCCCHHHHEEECCCCCCCCCEEEEEE
VADPATQAPSAEEIVAWCRERLAHFKCPRDVHFVAELPRNATGKLLKTELRAQFTGIEGG
EECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHEECCCCCCCCHHHHHHHHHHHCCCCCC
VVLR
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA