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The map label for this gene is gatA [H]

Identifier: 169629111

GI number: 169629111

Start: 2021053

End: 2022858

Strand: Direct

Name: gatA [H]

Synonym: MAB_2024

Alternate gene names: 169629111

Gene position: 2021053-2022858 (Clockwise)

Preceding gene: 169629109

Following gene: 169629112

Centisome position: 39.89

GC content: 63.51

Gene sequence:

>1806_bases
ATGAACTACTCCGGATTCACCGCACAGCCCACTTCCTCGACACGGCGCCCTGACGCTGCCCGTAACGCGGGGGAGTGCCG
CGGAAGACCGCTCTTTGAGGTGGTTGAGGCCTCGATTGCCGAGCTTCGCGCCGCACTGGAGTCGGGTGAGACGAGCAGTG
CCGAACTCACCCGCGCGTATCTGGAGCGCATCGATGCGTATGACAAGAGCGGTCCGACGCTGAATGCGCTGGTGGTGATA
AACCCAGACGCGATGCTCGAAGCTCGCGCATCCGACGCGCGCCGCGGCCGCGGTGAACTGCTCGGTCCTCTGGACGGCAT
CCCGTATACGGCCAAGGACAGCTATCTGGTGAAAGGCCTCACCGCTGCCGCTGGCTCGCCCGCTTTTGAACACCTTGTCG
CGCAACGAGATGCGTTCACGATCGAGCGGCTGCGTGCCGGTGGCGCGGTATTGATCGGGCTGACAAATATGCCGCCGATG
GCGAACGGTGGCATGCAGCGGGGAGTGTATGGGCGCGCGGAGAGTCCTTACAACGGTGATTTCTTAACGGCGGCGTTTGG
TTCCGGTTCATCCAACGGTTCTGGCACTGCCACCGCTGCCAGCTTCGCGGCGTTCGGGCTCGGTGAAGAGACCTGGTCGA
GTGGCCGTTCGCCGGCTTCGAACAACGCTCTTTGTGCCTACACTCCGTCGCGCGGCGTGATTTCAGTGCGTGGTAATTGG
CCGCTGGTCCCCACCATGGACGTGGTGGTGCCGCATACGCGTTCCATGGCCGACATGTTCGAGGTGCTCGATGTGATTGT
CGCCGACGATGCTGAAACACGCGGTGACTTCTGGCGCATGCAATCGTGGGTCGAGATTCCCGCCGCATCCACTGTGCGAC
CGGCGTCCTACCCGACGCTTGCCCCCGCGAGTGCGGCGAAAGCCCGTGCGGCTTTGAGGGGCAAGCGGTTTGGGGTGCCG
CGCATGTATGTCAACGCTGACCCTGAGGCTGGCACGGGCGAAAGCCCTGGCATCGGCGGGTCCACGGGGCAGCCCATCGT
CACGCGGTCGTCCGTGATGGATCTTTGGCGTGTTGCCAAGCTGGATTTGGAAAGGGCAGGGGCTGAGGTGATTCTGGTCG
ATTTTCCCGTGGTGTCGAACTACGAAGGAGACAGGCCCGGGGCACCGACGATCGGCACACGTGGACTGGTGTCCCCGGAA
TTCCTTCGTACCGAGACCGGACTGCTGGCAGCCTGGTCGTGGGATGACTTTCTGCGGGCCAACGACGACCCGGCCCTGAA
CCGTCTTGCGGACGTGAATGGAGAGATGATCTCTCCTCATCCGGCAGGGGCGTTGCCAGATCGCTATGCCGGATTTGACG
ATGACATCGCTGAATACCCAGCGTGCATTCGGGAAAATGCTGTCTCCGGGCTGGGCGCGATCCCGCACCTTCCGGAGGGT
CTGCGAGGACTGGAGGAGACCCGCCGTCTCGATCTGGAGGAGTGGATGGACGGACACCGGCTGGATGCCGTGATATTCCC
GGCCTCCGCCGATGTGGGGGCTGCGGATATGGACGTTGACCAGGAGTCGGCTGATAGGGGATGGCGTAACGGGGTCTGGG
TGGCCAACGGCAATCTCGTACCGCGTCACTTGGGCATCCCGACCGTGACCGTTCCGATGGGCACTATGTCGGATATCGGC
ATGCCTGTCGGGCTCACTTTCGCAGGGCGCAGCTACGACGACGCTGCTCTGCTCACCTTGGCTGCGGCGTTTGAAGCAAC
CGGGATGCGCCGAACCGTGCCTCCGCGCACCCCGCGGCTTGGCTAA

Upstream 100 bases:

>100_bases
CGATGAACAGCCCGGATCGCTGCGGGAACTGGGGATGGACGTCATCCCGGTCAGACACCGCCGAAGCTATACCGCCCCAC
ATACATTGAGGAACCACACA

Downstream 100 bases:

>100_bases
GTACTGGGCGGGGGCAGGTAGCACCTGCCCCCGCTCGTGTGGAGGTGCGGAACTACCCGACTCGCTCTTTCAACCCTGGA
AGGGAATTTCCATGACTGTG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 601; Mature: 601

Protein sequence:

>601_residues
MNYSGFTAQPTSSTRRPDAARNAGECRGRPLFEVVEASIAELRAALESGETSSAELTRAYLERIDAYDKSGPTLNALVVI
NPDAMLEARASDARRGRGELLGPLDGIPYTAKDSYLVKGLTAAAGSPAFEHLVAQRDAFTIERLRAGGAVLIGLTNMPPM
ANGGMQRGVYGRAESPYNGDFLTAAFGSGSSNGSGTATAASFAAFGLGEETWSSGRSPASNNALCAYTPSRGVISVRGNW
PLVPTMDVVVPHTRSMADMFEVLDVIVADDAETRGDFWRMQSWVEIPAASTVRPASYPTLAPASAAKARAALRGKRFGVP
RMYVNADPEAGTGESPGIGGSTGQPIVTRSSVMDLWRVAKLDLERAGAEVILVDFPVVSNYEGDRPGAPTIGTRGLVSPE
FLRTETGLLAAWSWDDFLRANDDPALNRLADVNGEMISPHPAGALPDRYAGFDDDIAEYPACIRENAVSGLGAIPHLPEG
LRGLEETRRLDLEEWMDGHRLDAVIFPASADVGAADMDVDQESADRGWRNGVWVANGNLVPRHLGIPTVTVPMGTMSDIG
MPVGLTFAGRSYDDAALLTLAAAFEATGMRRTVPPRTPRLG

Sequences:

>Translated_601_residues
MNYSGFTAQPTSSTRRPDAARNAGECRGRPLFEVVEASIAELRAALESGETSSAELTRAYLERIDAYDKSGPTLNALVVI
NPDAMLEARASDARRGRGELLGPLDGIPYTAKDSYLVKGLTAAAGSPAFEHLVAQRDAFTIERLRAGGAVLIGLTNMPPM
ANGGMQRGVYGRAESPYNGDFLTAAFGSGSSNGSGTATAASFAAFGLGEETWSSGRSPASNNALCAYTPSRGVISVRGNW
PLVPTMDVVVPHTRSMADMFEVLDVIVADDAETRGDFWRMQSWVEIPAASTVRPASYPTLAPASAAKARAALRGKRFGVP
RMYVNADPEAGTGESPGIGGSTGQPIVTRSSVMDLWRVAKLDLERAGAEVILVDFPVVSNYEGDRPGAPTIGTRGLVSPE
FLRTETGLLAAWSWDDFLRANDDPALNRLADVNGEMISPHPAGALPDRYAGFDDDIAEYPACIRENAVSGLGAIPHLPEG
LRGLEETRRLDLEEWMDGHRLDAVIFPASADVGAADMDVDQESADRGWRNGVWVANGNLVPRHLGIPTVTVPMGTMSDIG
MPVGLTFAGRSYDDAALLTLAAAFEATGMRRTVPPRTPRLG
>Mature_601_residues
MNYSGFTAQPTSSTRRPDAARNAGECRGRPLFEVVEASIAELRAALESGETSSAELTRAYLERIDAYDKSGPTLNALVVI
NPDAMLEARASDARRGRGELLGPLDGIPYTAKDSYLVKGLTAAAGSPAFEHLVAQRDAFTIERLRAGGAVLIGLTNMPPM
ANGGMQRGVYGRAESPYNGDFLTAAFGSGSSNGSGTATAASFAAFGLGEETWSSGRSPASNNALCAYTPSRGVISVRGNW
PLVPTMDVVVPHTRSMADMFEVLDVIVADDAETRGDFWRMQSWVEIPAASTVRPASYPTLAPASAAKARAALRGKRFGVP
RMYVNADPEAGTGESPGIGGSTGQPIVTRSSVMDLWRVAKLDLERAGAEVILVDFPVVSNYEGDRPGAPTIGTRGLVSPE
FLRTETGLLAAWSWDDFLRANDDPALNRLADVNGEMISPHPAGALPDRYAGFDDDIAEYPACIRENAVSGLGAIPHLPEG
LRGLEETRRLDLEEWMDGHRLDAVIFPASADVGAADMDVDQESADRGWRNGVWVANGNLVPRHLGIPTVTVPMGTMSDIG
MPVGLTFAGRSYDDAALLTLAAAFEATGMRRTVPPRTPRLG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=575, Percent_Identity=25.0434782608696, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI195972892, Length=224, Percent_Identity=27.2321428571429, Blast_Score=82, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=240, Percent_Identity=26.25, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=245, Percent_Identity=27.7551020408163, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=225, Percent_Identity=28, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24644968, Length=268, Percent_Identity=27.6119402985075, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI161078093, Length=259, Percent_Identity=30.5019305019305, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648113, Length=257, Percent_Identity=30.3501945525292, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356731, Length=245, Percent_Identity=27.7551020408163, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648435, Length=218, Percent_Identity=28.4403669724771, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648437, Length=218, Percent_Identity=28.4403669724771, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI45550774, Length=218, Percent_Identity=28.4403669724771, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24652985, Length=217, Percent_Identity=30.8755760368664, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19922090, Length=217, Percent_Identity=30.8755760368664, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24652981, Length=217, Percent_Identity=30.8755760368664, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24652983, Length=217, Percent_Identity=30.8755760368664, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 63797; Mature: 63797

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYSGFTAQPTSSTRRPDAARNAGECRGRPLFEVVEASIAELRAALESGETSSAELTRAY
CCCCCCCCCCCCCCCCCCHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LERIDAYDKSGPTLNALVVINPDAMLEARASDARRGRGELLGPLDGIPYTAKDSYLVKGL
HHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
TAAAGSPAFEHLVAQRDAFTIERLRAGGAVLIGLTNMPPMANGGMQRGVYGRAESPYNGD
HHCCCCHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
FLTAAFGSGSSNGSGTATAASFAAFGLGEETWSSGRSPASNNALCAYTPSRGVISVRGNW
EEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCCCCEEEECCCC
PLVPTMDVVVPHTRSMADMFEVLDVIVADDAETRGDFWRMQSWVEIPAASTVRPASYPTL
CCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
APASAAKARAALRGKRFGVPRMYVNADPEAGTGESPGIGGSTGQPIVTRSSVMDLWRVAK
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHH
LDLERAGAEVILVDFPVVSNYEGDRPGAPTIGTRGLVSPEFLRTETGLLAAWSWDDFLRA
HHHHHCCCEEEEEECCEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCHHHHCC
NDDPALNRLADVNGEMISPHPAGALPDRYAGFDDDIAEYPACIRENAVSGLGAIPHLPEG
CCCHHHHHHHHCCCCEECCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHH
LRGLEETRRLDLEEWMDGHRLDAVIFPASADVGAADMDVDQESADRGWRNGVWVANGNLV
HHHHHHHHCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCEEEECCCCC
PRHLGIPTVTVPMGTMSDIGMPVGLTFAGRSYDDAALLTLAAAFEATGMRRTVPPRTPRL
CCCCCCCEEEECCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
G
C
>Mature Secondary Structure
MNYSGFTAQPTSSTRRPDAARNAGECRGRPLFEVVEASIAELRAALESGETSSAELTRAY
CCCCCCCCCCCCCCCCCCHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
LERIDAYDKSGPTLNALVVINPDAMLEARASDARRGRGELLGPLDGIPYTAKDSYLVKGL
HHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
TAAAGSPAFEHLVAQRDAFTIERLRAGGAVLIGLTNMPPMANGGMQRGVYGRAESPYNGD
HHCCCCHHHHHHHHHCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
FLTAAFGSGSSNGSGTATAASFAAFGLGEETWSSGRSPASNNALCAYTPSRGVISVRGNW
EEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCCCCEEEECCCC
PLVPTMDVVVPHTRSMADMFEVLDVIVADDAETRGDFWRMQSWVEIPAASTVRPASYPTL
CCCCCCCEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
APASAAKARAALRGKRFGVPRMYVNADPEAGTGESPGIGGSTGQPIVTRSSVMDLWRVAK
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHH
LDLERAGAEVILVDFPVVSNYEGDRPGAPTIGTRGLVSPEFLRTETGLLAAWSWDDFLRA
HHHHHCCCEEEEEECCEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEECCHHHHCC
NDDPALNRLADVNGEMISPHPAGALPDRYAGFDDDIAEYPACIRENAVSGLGAIPHLPEG
CCCHHHHHHHHCCCCEECCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHH
LRGLEETRRLDLEEWMDGHRLDAVIFPASADVGAADMDVDQESADRGWRNGVWVANGNLV
HHHHHHHHCCCHHHHCCCCCEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCEEEECCCCC
PRHLGIPTVTVPMGTMSDIGMPVGLTFAGRSYDDAALLTLAAAFEATGMRRTVPPRTPRL
CCCCCCCEEEECCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA