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The map label for this gene is metF [H]

Identifier: 169629080

GI number: 169629080

Start: 1990758

End: 1991738

Strand: Direct

Name: metF [H]

Synonym: MAB_1993

Alternate gene names: 169629080

Gene position: 1990758-1991738 (Clockwise)

Preceding gene: 169629077

Following gene: 169629083

Centisome position: 39.29

GC content: 65.75

Gene sequence:

>981_bases
GTGACAACCAACCCGCTCGACCGCGATGGACCGGTCGCCGAGCTGCAGGATCCGGGGTCGATCGCCGATCGGCTCACCGC
GGTCCAGCCCGGCGAGATCGCGTTCTCGGTCGAGTTCATGCCGCCCCGGGACGAGGCGGCTGAGGCTCGTTTGTGGCGGG
CGGCAAGAGTTTTCGAGCGGTACCGGCCAGTGTTCGTTTCGGTCACCTACGGTGCGGGCGGTTCCACCCGTGACCGCACG
GTGCGGGTGACCGGTGAGCTTGCCGAGAACACCACGTTGCTGCCGGTCGCGCATCTGACGGCCGTCGGACACACCGTCGA
TGAGCTGCGCGCCATGGTGGGTGCGTATGTCGATCGCGGTATCACCAATATCCTTGCCCTGCGCGGCGATCCGGCCGGCG
ACGTGAACGCCGAGTGGGTACCGCATCCGGGCGGACTGACCTACGCCGAACAACTGGTGCGGTTGGTCCGCGACCTCGGC
GATTTCCATGTCGGGGTCGCCTCTTTCCCCGAGGGGCATCCGCAAGCGATCGACCTGGAAAGCGATACCGCCAATCTGGT
GAACAAGTTGCGTGCGGGTGCCGAGTACTCCATCACGCAGATGTTTTTCGACGTCGACGACTATCTCAGGCTGCGCGACC
GGGTGGTAGCGGCAGATCCGGAGCAGGGGGCCAAGCCCATTGTCCCCGGCCTGATGCCCATCACCTCGCTGCGTTCGGTA
CGTCGTCAGGTGGAGCTTTCCTCGTCCACGCTGCCCACGGCATTGGAGGAGCGGCTGCTCAGGGCGGCGGGCGACGGTCC
GGAAGAGAACCGCGACGAGGTCCGCAAGGTGGGTGTCGAGGTCACCACCACGATGGCTTCGCGGCTGCTGGCCGAGGGTG
TGCCGTGTCTGCACTTCATGACCCTCAACTTTGCGCGGGCCACCTCAGAGGTGTTGGAGAATCTCGGGGTGAGGGTTACT
CCGGGAACTGCTCGGGGGTAA

Upstream 100 bases:

>100_bases
GGCTACGTTGACACTCAGCGCGGCGTCCTTTCACGGTGCGGTAATTCAGTCCCGGATGTGCGGGGAGTGAACCTAGAGTA
ATCCCACCTATCCTGAACAG

Downstream 100 bases:

>100_bases
ACCGCGGCGATTGGTCGCGGTCTAGCCACGCCATGGAGGCGACGAGGGCGCCGACCAGCAGAGCGGCGATCGATACGAAG
ATCGCGGCACCATTGAACGA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTTNPLDRDGPVAELQDPGSIADRLTAVQPGEIAFSVEFMPPRDEAAEARLWRAARVFERYRPVFVSVTYGAGGSTRDRT
VRVTGELAENTTLLPVAHLTAVGHTVDELRAMVGAYVDRGITNILALRGDPAGDVNAEWVPHPGGLTYAEQLVRLVRDLG
DFHVGVASFPEGHPQAIDLESDTANLVNKLRAGAEYSITQMFFDVDDYLRLRDRVVAADPEQGAKPIVPGLMPITSLRSV
RRQVELSSSTLPTALEERLLRAAGDGPEENRDEVRKVGVEVTTTMASRLLAEGVPCLHFMTLNFARATSEVLENLGVRVT
PGTARG

Sequences:

>Translated_326_residues
MTTNPLDRDGPVAELQDPGSIADRLTAVQPGEIAFSVEFMPPRDEAAEARLWRAARVFERYRPVFVSVTYGAGGSTRDRT
VRVTGELAENTTLLPVAHLTAVGHTVDELRAMVGAYVDRGITNILALRGDPAGDVNAEWVPHPGGLTYAEQLVRLVRDLG
DFHVGVASFPEGHPQAIDLESDTANLVNKLRAGAEYSITQMFFDVDDYLRLRDRVVAADPEQGAKPIVPGLMPITSLRSV
RRQVELSSSTLPTALEERLLRAAGDGPEENRDEVRKVGVEVTTTMASRLLAEGVPCLHFMTLNFARATSEVLENLGVRVT
PGTARG
>Mature_325_residues
TTNPLDRDGPVAELQDPGSIADRLTAVQPGEIAFSVEFMPPRDEAAEARLWRAARVFERYRPVFVSVTYGAGGSTRDRTV
RVTGELAENTTLLPVAHLTAVGHTVDELRAMVGAYVDRGITNILALRGDPAGDVNAEWVPHPGGLTYAEQLVRLVRDLGD
FHVGVASFPEGHPQAIDLESDTANLVNKLRAGAEYSITQMFFDVDDYLRLRDRVVAADPEQGAKPIVPGLMPITSLRSVR
RQVELSSSTLPTALEERLLRAAGDGPEENRDEVRKVGVEVTTTMASRLLAEGVPCLHFMTLNFARATSEVLENLGVRVTP
GTARG

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=301, Percent_Identity=35.5481727574751, Blast_Score=163, Evalue=2e-40,
Organism=Escherichia coli, GI1790377, Length=301, Percent_Identity=37.5415282392027, Blast_Score=189, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI25147441, Length=309, Percent_Identity=30.42071197411, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25147436, Length=309, Percent_Identity=30.42071197411, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321313, Length=302, Percent_Identity=31.4569536423841, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6325234, Length=302, Percent_Identity=32.4503311258278, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 35404; Mature: 35273

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNPLDRDGPVAELQDPGSIADRLTAVQPGEIAFSVEFMPPRDEAAEARLWRAARVFER
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
YRPVFVSVTYGAGGSTRDRTVRVTGELAENTTLLPVAHLTAVGHTVDELRAMVGAYVDRG
CCCEEEEEEECCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHH
ITNILALRGDPAGDVNAEWVPHPGGLTYAEQLVRLVRDLGDFHVGVASFPEGHPQAIDLE
HHHEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC
SDTANLVNKLRAGAEYSITQMFFDVDDYLRLRDRVVAADPEQGAKPIVPGLMPITSLRSV
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHH
RRQVELSSSTLPTALEERLLRAAGDGPEENRDEVRKVGVEVTTTMASRLLAEGVPCLHFM
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHHCCCCHHHHH
TLNFARATSEVLENLGVRVTPGTARG
HHHHHHHHHHHHHHCCCEECCCCCCC
>Mature Secondary Structure 
TTNPLDRDGPVAELQDPGSIADRLTAVQPGEIAFSVEFMPPRDEAAEARLWRAARVFER
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
YRPVFVSVTYGAGGSTRDRTVRVTGELAENTTLLPVAHLTAVGHTVDELRAMVGAYVDRG
CCCEEEEEEECCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHCCHHHHHHHHHHHHHHH
ITNILALRGDPAGDVNAEWVPHPGGLTYAEQLVRLVRDLGDFHVGVASFPEGHPQAIDLE
HHHEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC
SDTANLVNKLRAGAEYSITQMFFDVDDYLRLRDRVVAADPEQGAKPIVPGLMPITSLRSV
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHH
RRQVELSSSTLPTALEERLLRAAGDGPEENRDEVRKVGVEVTTTMASRLLAEGVPCLHFM
HHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHHHHHHHCCCCHHHHH
TLNFARATSEVLENLGVRVTPGTARG
HHHHHHHHHHHHHHCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9515933 [H]