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The map label for this gene is nfdA [H]

Identifier: 169628956

GI number: 169628956

Start: 1866638

End: 1868389

Strand: Reverse

Name: nfdA [H]

Synonym: MAB_1868c

Alternate gene names: 169628956

Gene position: 1868389-1866638 (Counterclockwise)

Preceding gene: 169628957

Following gene: 169628954

Centisome position: 36.87

GC content: 67.07

Gene sequence:

>1752_bases
ATGTCGATCAATCGGAGGCAGTTCGTGGTCGGCGGAGCCGCCGCTACCGCTGCCACCACGGGACTGGCGGCACTGGCCGG
GTGCGGCCACGACACCACCGATCCCGATACCGGTGCCGACCTGATCTTTGTCGGTGGCCCGGTGGTGACGGTCGATGGGC
ACGGCACCACGACCGAGGCACTGGCCGTTGCCCAGGGCAAGATCGCCGCGGTCGGTTCACGGCAGGACGTGATGAGGCTG
CGCGGTGCCCAAACGGATGTGATCGAGCTCGACGGCCTGGCGCTGCTTCCGGCGTTCGTGGAGGCGCACAGCCACCCGAG
CCAGATCGCCGCCACCCTCGGTCCGCCCGCCGTGGACGTGCGCCCTTTCGTCGTTCCCACCGGTCAGCAGGTCATGCAGA
AGCTGGCCGACACCGTCCGGAGTACCCCGAAGGGTACCCAGGTACTGCTCTACGGCATCGACGTGCTGCTGCAGCCGGAT
CTCCAATTACCTACGCGCACAATTCTTGACGCGCTGGCTCCGGACCATCCGGTGATCATCGTCGCCAACAGCGGACACGC
CGCCTACGCCAACACAGCTTCCTTCCGGCTCGCCGGAATCACCAGGGACACACCCAATCCCGTCGGCGCCGAATACGTCC
ACGGACCCGACGGCGAGCTCACCGGCGAGGTTCGCGAGGCCGCCGCGCTGACCACCTTGGCAATGCCGTATCTACGGAAT
GTCGCCGAGCCCAACGCCTTCCAGAATCTGCACTGGGCGTTTGGGCAGCTGGCCGCCGTGGGCATCGCCACCGCCAGCGA
GCACAGCTACAGCGCCGCGCAGCAGGGCGACCTCTACCGCAGGATGGCCGCCGATCCGGGTGTCAAACTGCGCATCCGCG
CCTATGAGATGGGCGCCCCCGAGCTGGCCAAGGACCCCACGCATGTCGCCGGGCCGGCGGCCGGCGCCGATCAGCTGTTC
GCGCAGATCGGCATGAAGCTGTGGGCAGACGGATCACCCTGGCAGGGAAATATTTTCACCTCGTTCCCCTACCTGGACAC
CGCGGCTACGCGCCGCATGGGCCTGGAGCCACACCATCACGGCGGCATGAACTACACGCCGGCCCAGGTCACCGAGCTGG
CGACGGCCTTCATCGAGCAGGGCTGGCAGCTGGCGTGTCACGTCCACGGTGACCGCGCGATCGATGTGGTGCTCGACGCG
TATGAGAAGGCCCAGTCCCCCGCCTCGCTACGCTCCCGCCTGGAACATGTGGGCGCGATGACTCCCGCACAGTTCCAGCG
CGCGGCACGCCTGGGTGTGCATCCGAGCCTGTTCATCGAACATGTCTACTTCTGGGGCGACCCACTCATCGACGAGCTGT
TCGGTCAAGACCATGGCGCGCACTGGATGTCGGCAAAATCTGCGCTGGACGCCGGGCTCAGAATCTCGTTTCACAACGAT
GGCAACGTGTCCCCGCCGAACCCGCTGGGCAACATCGCCACCGCCGTCACCCGCACGGCCAAGGGCTCCGGCCGTGTGCT
GGCCCCCGAACAACGGATCAGCGTCACGCAGGCCGTAAGAGCCCAGACCATCGACGCGGCCTGGCAGCTGCATCTCGACA
ACGAGGTCGGCAGTCTGGAAGTGGGCAAGTACGCCGACCTGGTGGTGTTATCCGCCGACCCGCACGCGGTAGACCCGCAG
GCACTGCGCGATATCTCGGTGCGCGCCACCTACCTGGCCGGTCGGCAGACCTATGCCGCACCCGTCGGCTGA

Upstream 100 bases:

>100_bases
GTCTCCGAGCGCATCGGCATCGAAAGCATCGAAGCCGCGTTCGAAAAGATGCACCATGGCGAGGTCCTGCGCTCCGTAGT
GGTGCTCTAGCCGCACTCCG

Downstream 100 bases:

>100_bases
CTCGCACCCTCAGCGCTGCGGGTCCAGCATTCCGCGGGTCTTCTTGGCCTCGTACAGCGCATGGTCGGCTTGCTGCACAA
GCGATTCCAGGTCGACCTTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MSINRRQFVVGGAAATAATTGLAALAGCGHDTTDPDTGADLIFVGGPVVTVDGHGTTTEALAVAQGKIAAVGSRQDVMRL
RGAQTDVIELDGLALLPAFVEAHSHPSQIAATLGPPAVDVRPFVVPTGQQVMQKLADTVRSTPKGTQVLLYGIDVLLQPD
LQLPTRTILDALAPDHPVIIVANSGHAAYANTASFRLAGITRDTPNPVGAEYVHGPDGELTGEVREAAALTTLAMPYLRN
VAEPNAFQNLHWAFGQLAAVGIATASEHSYSAAQQGDLYRRMAADPGVKLRIRAYEMGAPELAKDPTHVAGPAAGADQLF
AQIGMKLWADGSPWQGNIFTSFPYLDTAATRRMGLEPHHHGGMNYTPAQVTELATAFIEQGWQLACHVHGDRAIDVVLDA
YEKAQSPASLRSRLEHVGAMTPAQFQRAARLGVHPSLFIEHVYFWGDPLIDELFGQDHGAHWMSAKSALDAGLRISFHND
GNVSPPNPLGNIATAVTRTAKGSGRVLAPEQRISVTQAVRAQTIDAAWQLHLDNEVGSLEVGKYADLVVLSADPHAVDPQ
ALRDISVRATYLAGRQTYAAPVG

Sequences:

>Translated_583_residues
MSINRRQFVVGGAAATAATTGLAALAGCGHDTTDPDTGADLIFVGGPVVTVDGHGTTTEALAVAQGKIAAVGSRQDVMRL
RGAQTDVIELDGLALLPAFVEAHSHPSQIAATLGPPAVDVRPFVVPTGQQVMQKLADTVRSTPKGTQVLLYGIDVLLQPD
LQLPTRTILDALAPDHPVIIVANSGHAAYANTASFRLAGITRDTPNPVGAEYVHGPDGELTGEVREAAALTTLAMPYLRN
VAEPNAFQNLHWAFGQLAAVGIATASEHSYSAAQQGDLYRRMAADPGVKLRIRAYEMGAPELAKDPTHVAGPAAGADQLF
AQIGMKLWADGSPWQGNIFTSFPYLDTAATRRMGLEPHHHGGMNYTPAQVTELATAFIEQGWQLACHVHGDRAIDVVLDA
YEKAQSPASLRSRLEHVGAMTPAQFQRAARLGVHPSLFIEHVYFWGDPLIDELFGQDHGAHWMSAKSALDAGLRISFHND
GNVSPPNPLGNIATAVTRTAKGSGRVLAPEQRISVTQAVRAQTIDAAWQLHLDNEVGSLEVGKYADLVVLSADPHAVDPQ
ALRDISVRATYLAGRQTYAAPVG
>Mature_582_residues
SINRRQFVVGGAAATAATTGLAALAGCGHDTTDPDTGADLIFVGGPVVTVDGHGTTTEALAVAQGKIAAVGSRQDVMRLR
GAQTDVIELDGLALLPAFVEAHSHPSQIAATLGPPAVDVRPFVVPTGQQVMQKLADTVRSTPKGTQVLLYGIDVLLQPDL
QLPTRTILDALAPDHPVIIVANSGHAAYANTASFRLAGITRDTPNPVGAEYVHGPDGELTGEVREAAALTTLAMPYLRNV
AEPNAFQNLHWAFGQLAAVGIATASEHSYSAAQQGDLYRRMAADPGVKLRIRAYEMGAPELAKDPTHVAGPAAGADQLFA
QIGMKLWADGSPWQGNIFTSFPYLDTAATRRMGLEPHHHGGMNYTPAQVTELATAFIEQGWQLACHVHGDRAIDVVLDAY
EKAQSPASLRSRLEHVGAMTPAQFQRAARLGVHPSLFIEHVYFWGDPLIDELFGQDHGAHWMSAKSALDAGLRISFHNDG
NVSPPNPLGNIATAVTRTAKGSGRVLAPEQRISVTQAVRAQTIDAAWQLHLDNEVGSLEVGKYADLVVLSADPHAVDPQA
LRDISVRATYLAGRQTYAAPVG

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 61736; Mature: 61605

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSINRRQFVVGGAAATAATTGLAALAGCGHDTTDPDTGADLIFVGGPVVTVDGHGTTTEA
CCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEEEECCCCCCHHH
LAVAQGKIAAVGSRQDVMRLRGAQTDVIELDGLALLPAFVEAHSHPSQIAATLGPPAVDV
HHECCCCEEECCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCCC
RPFVVPTGQQVMQKLADTVRSTPKGTQVLLYGIDVLLQPDLQLPTRTILDALAPDHPVII
EEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHCCCCCEEE
VANSGHAAYANTASFRLAGITRDTPNPVGAEYVHGPDGELTGEVREAAALTTLAMPYLRN
EECCCCEEEECCCEEEEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
VAEPNAFQNLHWAFGQLAAVGIATASEHSYSAAQQGDLYRRMAADPGVKLRIRAYEMGAP
CCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCC
ELAKDPTHVAGPAAGADQLFAQIGMKLWADGSPWQGNIFTSFPYLDTAATRRMGLEPHHH
HHCCCCCCCCCCCCCHHHHHHHHCHHEECCCCCCCCCEEECCCCHHHHHHHHCCCCCCCC
GGMNYTPAQVTELATAFIEQGWQLACHVHGDRAIDVVLDAYEKAQSPASLRSRLEHVGAM
CCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHHHHHHHCCCCHHHHHHHHHHCCC
TPAQFQRAARLGVHPSLFIEHVYFWGDPLIDELFGQDHGAHWMSAKSALDAGLRISFHND
CHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCC
GNVSPPNPLGNIATAVTRTAKGSGRVLAPEQRISVTQAVRAQTIDAAWQLHLDNEVGSLE
CCCCCCCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCEEEEEEECCCCCCEE
VGKYADLVVLSADPHAVDPQALRDISVRATYLAGRQTYAAPVG
CCCCCCEEEECCCCCCCCHHHHHHHHEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
SINRRQFVVGGAAATAATTGLAALAGCGHDTTDPDTGADLIFVGGPVVTVDGHGTTTEA
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEEEECCCCCCHHH
LAVAQGKIAAVGSRQDVMRLRGAQTDVIELDGLALLPAFVEAHSHPSQIAATLGPPAVDV
HHECCCCEEECCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCCC
RPFVVPTGQQVMQKLADTVRSTPKGTQVLLYGIDVLLQPDLQLPTRTILDALAPDHPVII
EEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHCCCCCEEE
VANSGHAAYANTASFRLAGITRDTPNPVGAEYVHGPDGELTGEVREAAALTTLAMPYLRN
EECCCCEEEECCCEEEEEEECCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHH
VAEPNAFQNLHWAFGQLAAVGIATASEHSYSAAQQGDLYRRMAADPGVKLRIRAYEMGAP
CCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCC
ELAKDPTHVAGPAAGADQLFAQIGMKLWADGSPWQGNIFTSFPYLDTAATRRMGLEPHHH
HHCCCCCCCCCCCCCHHHHHHHHCHHEECCCCCCCCCEEECCCCHHHHHHHHCCCCCCCC
GGMNYTPAQVTELATAFIEQGWQLACHVHGDRAIDVVLDAYEKAQSPASLRSRLEHVGAM
CCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHHHHHHHCCCCHHHHHHHHHHCCC
TPAQFQRAARLGVHPSLFIEHVYFWGDPLIDELFGQDHGAHWMSAKSALDAGLRISFHND
CHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHCCCCEEEECCC
GNVSPPNPLGNIATAVTRTAKGSGRVLAPEQRISVTQAVRAQTIDAAWQLHLDNEVGSLE
CCCCCCCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCEEEEEEECCCCCCEE
VGKYADLVVLSADPHAVDPQALRDISVRATYLAGRQTYAAPVG
CCCCCCEEEECCCCCCCCHHHHHHHHEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA