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The map label for this gene is mmcV [H]

Identifier: 169628750

GI number: 169628750

Start: 1688012

End: 1688938

Strand: Reverse

Name: mmcV [H]

Synonym: MAB_1660c

Alternate gene names: 169628750

Gene position: 1688938-1688012 (Counterclockwise)

Preceding gene: 169628751

Following gene: 169628749

Centisome position: 33.33

GC content: 64.94

Gene sequence:

>927_bases
ATGACGACTGAAATCGGAACGGAAGCTGTGTTGAACCAGACAGAGACACTCACCGACCTGGAATCCGAATCGATCCACAT
CATCCGCGAGGTGGCCGGCGAGTTCGAGCGCCCGGTGCTGTTGTTCTCCGGCGGCAAGGATTCGACGGTGCTGCTGCATA
TCGCACTCAAGGCCTTTTGGCCGGCACCCCTGCCTTTTTCGCTGTTGCACGTCGACACCGGGCACAACCTCCCCGAGGTG
CTGGCCTTCCGCGACGAGATCGTCGAGCGCCACAACCTGCGCCTGGTGGTCTCGAACGTCGAGGATTACCTGGCCGACGG
CCGTCTGACCGAGCGCCCGGACGGAATCCGCAATCCCCTGCAGACGATTCCGCTGCTCGAGGCCATCACCGACAACAAGT
TCGACGCGGTGCTGGGTGGCGGGCGCCGCGACGAGGAGCGTTCGCGCGCCAAGGAGCGAATCTTCAGCCTGCGCAATGCC
TTCGGCCAGTGGGATCCGAAGAAGCAGCGTCCCGAGCTGTGGAGCCTGTACAACGGCCGGCATGCTCCGGGCGAGCACGT
CCGGGTGTTCCCGATCAGTAACTGGACCGAGCTGGACGTGTGGCGTTACATCGCCCGCGAGAACGTGCAGCTGGCCAGCC
TCTACTACGCGCATGAGCGCGAGGTGTTCAACCGCGACGGCATGCTGCTGACCCCCGGCCCGTGGGGCGGACCACGGGAC
GGTGAAGAGCTGCAGACACTTTCGGTGCGCTATCGGACCGTCGGCGACGGTTCGACCACCGGTGCTGTGCTCTCCGACGC
CGCCGATGTGCATGCGGTGCTGGCCGAGGTGGCTTCCTCCCGGATCACCGAACGCGGCGCCACCCGTGGCGATGACCGAG
TGTCCGAGGCGGCGATGGAAGACCGCAAGCGAGAGGGTTATTTCTGA

Upstream 100 bases:

>100_bases
TCGGCTGCGCCCCGTGCACGGCCAAACCGGTACTCGGCGGCGACGCACGAAGCGGACGCTGGCAGGGCCTTGCCAAGACA
GAATGCGGGTTGCACGCCTC

Downstream 100 bases:

>100_bases
TCATGAGCGATCTTCTGCGCATTGCCACCGCCGGCAGTGTGGACGACGGAAAGTCCACTCTGGTGGGCCGGCTGCTGTAC
GACACCAAGTCGGTGCTCAT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Mitomycin biosynthesis protein V; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTTEIGTEAVLNQTETLTDLESESIHIIREVAGEFERPVLLFSGGKDSTVLLHIALKAFWPAPLPFSLLHVDTGHNLPEV
LAFRDEIVERHNLRLVVSNVEDYLADGRLTERPDGIRNPLQTIPLLEAITDNKFDAVLGGGRRDEERSRAKERIFSLRNA
FGQWDPKKQRPELWSLYNGRHAPGEHVRVFPISNWTELDVWRYIARENVQLASLYYAHEREVFNRDGMLLTPGPWGGPRD
GEELQTLSVRYRTVGDGSTTGAVLSDAADVHAVLAEVASSRITERGATRGDDRVSEAAMEDRKREGYF

Sequences:

>Translated_308_residues
MTTEIGTEAVLNQTETLTDLESESIHIIREVAGEFERPVLLFSGGKDSTVLLHIALKAFWPAPLPFSLLHVDTGHNLPEV
LAFRDEIVERHNLRLVVSNVEDYLADGRLTERPDGIRNPLQTIPLLEAITDNKFDAVLGGGRRDEERSRAKERIFSLRNA
FGQWDPKKQRPELWSLYNGRHAPGEHVRVFPISNWTELDVWRYIARENVQLASLYYAHEREVFNRDGMLLTPGPWGGPRD
GEELQTLSVRYRTVGDGSTTGAVLSDAADVHAVLAEVASSRITERGATRGDDRVSEAAMEDRKREGYF
>Mature_307_residues
TTEIGTEAVLNQTETLTDLESESIHIIREVAGEFERPVLLFSGGKDSTVLLHIALKAFWPAPLPFSLLHVDTGHNLPEVL
AFRDEIVERHNLRLVVSNVEDYLADGRLTERPDGIRNPLQTIPLLEAITDNKFDAVLGGGRRDEERSRAKERIFSLRNAF
GQWDPKKQRPELWSLYNGRHAPGEHVRVFPISNWTELDVWRYIARENVQLASLYYAHEREVFNRDGMLLTPGPWGGPRDG
EELQTLSVRYRTVGDGSTTGAVLSDAADVHAVLAEVASSRITERGATRGDDRVSEAAMEDRKREGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=295, Percent_Identity=47.4576271186441, Blast_Score=253, Evalue=9e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34725; Mature: 34594

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEIGTEAVLNQTETLTDLESESIHIIREVAGEFERPVLLFSGGKDSTVLLHIALKAFW
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEHHHHC
PAPLPFSLLHVDTGHNLPEVLAFRDEIVERHNLRLVVSNVEDYLADGRLTERPDGIRNPL
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCCCCHHCCHH
QTIPLLEAITDNKFDAVLGGGRRDEERSRAKERIFSLRNAFGQWDPKKQRPELWSLYNGR
HHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCC
HAPGEHVRVFPISNWTELDVWRYIARENVQLASLYYAHEREVFNRDGMLLTPGPWGGPRD
CCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
GEELQTLSVRYRTVGDGSTTGAVLSDAADVHAVLAEVASSRITERGATRGDDRVSEAAME
CHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
DRKREGYF
HHHHCCCC
>Mature Secondary Structure 
TTEIGTEAVLNQTETLTDLESESIHIIREVAGEFERPVLLFSGGKDSTVLLHIALKAFW
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEEEEEHHHHC
PAPLPFSLLHVDTGHNLPEVLAFRDEIVERHNLRLVVSNVEDYLADGRLTERPDGIRNPL
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHCCCCCCCCCHHCCHH
QTIPLLEAITDNKFDAVLGGGRRDEERSRAKERIFSLRNAFGQWDPKKQRPELWSLYNGR
HHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCC
HAPGEHVRVFPISNWTELDVWRYIARENVQLASLYYAHEREVFNRDGMLLTPGPWGGPRD
CCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
GEELQTLSVRYRTVGDGSTTGAVLSDAADVHAVLAEVASSRITERGATRGDDRVSEAAME
CHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
DRKREGYF
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10099135 [H]