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The map label for this gene is tcmP [H]

Identifier: 169628608

GI number: 169628608

Start: 1536709

End: 1537539

Strand: Reverse

Name: tcmP [H]

Synonym: MAB_1517c

Alternate gene names: 169628608

Gene position: 1537539-1536709 (Counterclockwise)

Preceding gene: 169628611

Following gene: 169628606

Centisome position: 30.34

GC content: 61.49

Gene sequence:

>831_bases
ATGGGCATCGACGTCAGCGGAATCACCCCCGAGGAAGAGACCGCCTTTCTCACCCTGAAGGCCAGGGCCGTCAACGACGG
CTGGCGGCGGCCGATCCTGCGGGATCCGGGTGCCACCGCCGCCGTCTCCAAAATCGACTACGACTTCCACGCACTCGGGG
TGATTACCCCGGTGGTATGCCAATCATCGCTACGCGCAAAGCTCCTCGACGACCGAGTGCGCGCCTTTGTCACCGAGCAC
CCGAACGCGGTGGTAGTGGACATGGGCGCGGGCCTCGACGACGGATACCGACGTATTCAGCCACCGGCCACCGTCGACTG
GTACAGCGTTGACCTGCCCGGGATGGCCACACTGCGCGATGAAGTGATGCCCCCTGGCCCGGGCGAACACACCGTCGGGA
TATCTATTACCGATCCGAATTGGCTTGGCGGGATACCGTCGGATCGTCCGGCGATGGTGATCGCCGACGGGTTCTTCGCT
TTCCTGACCAAGGAACAGATCATCGCCACCCTGCGGGCCATCACCGACCACTTCAGCACTGGGCAACTGGCATTCAATGA
CTACGGACGCATGGTGGTGGGGGTATGGATCTCCAAGCTGTTCCCACAAAAGATGTTCAAGAAGGTCAATCAGCTGCGGG
GATTCGAGGGCTATAACGATCCGCGCACGCCGGAACGCTGGAACCCCCGGCTGCGATTTGTCGAGGAGTTCAATCTGGTG
GATGCGCCCGAGATCGAGTTGTTTCCCACCTGGTTGCGGATTTCGTCACAACTGGCGCGGTACAGCACCTCGATGAAGCG
TTCGGCCCGCATCCTGCGGTTCGAGTTCTAG

Upstream 100 bases:

>100_bases
CGTATCCGGGCACGGGCGGTGAGGTCGTCGGGATCCACGGTTTCCATCGCCATATGCTACACGACTGGACAATTGACTAC
ACGCTTGTATAGTCGAATCC

Downstream 100 bases:

>100_bases
ACCACAAATAACCGCTACATCAGTCGCGGCGGAACTTGCCGGCGACCTTGCGGACCACCAGCACCGCAACCACGGCACCG
AAGCCCAGGATGGTGAATTT

Product: O-methyltransferase Omt

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MGIDVSGITPEEETAFLTLKARAVNDGWRRPILRDPGATAAVSKIDYDFHALGVITPVVCQSSLRAKLLDDRVRAFVTEH
PNAVVVDMGAGLDDGYRRIQPPATVDWYSVDLPGMATLRDEVMPPGPGEHTVGISITDPNWLGGIPSDRPAMVIADGFFA
FLTKEQIIATLRAITDHFSTGQLAFNDYGRMVVGVWISKLFPQKMFKKVNQLRGFEGYNDPRTPERWNPRLRFVEEFNLV
DAPEIELFPTWLRISSQLARYSTSMKRSARILRFEF

Sequences:

>Translated_276_residues
MGIDVSGITPEEETAFLTLKARAVNDGWRRPILRDPGATAAVSKIDYDFHALGVITPVVCQSSLRAKLLDDRVRAFVTEH
PNAVVVDMGAGLDDGYRRIQPPATVDWYSVDLPGMATLRDEVMPPGPGEHTVGISITDPNWLGGIPSDRPAMVIADGFFA
FLTKEQIIATLRAITDHFSTGQLAFNDYGRMVVGVWISKLFPQKMFKKVNQLRGFEGYNDPRTPERWNPRLRFVEEFNLV
DAPEIELFPTWLRISSQLARYSTSMKRSARILRFEF
>Mature_275_residues
GIDVSGITPEEETAFLTLKARAVNDGWRRPILRDPGATAAVSKIDYDFHALGVITPVVCQSSLRAKLLDDRVRAFVTEHP
NAVVVDMGAGLDDGYRRIQPPATVDWYSVDLPGMATLRDEVMPPGPGEHTVGISITDPNWLGGIPSDRPAMVIADGFFAF
LTKEQIIATLRAITDHFSTGQLAFNDYGRMVVGVWISKLFPQKMFKKVNQLRGFEGYNDPRTPERWNPRLRFVEEFNLVD
APEIELFPTWLRISSQLARYSTSMKRSARILRFEF

Specific function: O-methyltransferase that catalyzes the methylation of the C-9 carboxy group of tetracenomycin E (TCM E) to yield TCM A2. Catalyzes as well the following side reactions:methylation of 8- O-methyl-TCM D3 to 9-carboxymethyl-8-O-methyl-TCM D3; and of TCM B3 t

COG id: COG3315

COG function: function code Q; O-Methyltransferase involved in polyketide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007213
- InterPro:   IPR016874 [H]

Pfam domain/function: PF04072 LCM [H]

EC number: NA

Molecular weight: Translated: 31015; Mature: 30884

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIDVSGITPEEETAFLTLKARAVNDGWRRPILRDPGATAAVSKIDYDFHALGVITPVVC
CCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHH
QSSLRAKLLDDRVRAFVTEHPNAVVVDMGAGLDDGYRRIQPPATVDWYSVDLPGMATLRD
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCEEEEEECCCCHHHHHH
EVMPPGPGEHTVGISITDPNWLGGIPSDRPAMVIADGFFAFLTKEQIIATLRAITDHFST
HCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCC
GQLAFNDYGRMVVGVWISKLFPQKMFKKVNQLRGFEGYNDPRTPERWNPRLRFVEEFNLV
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHCCCC
DAPEIELFPTWLRISSQLARYSTSMKRSARILRFEF
CCCCCEEEHHHHHHHHHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure 
GIDVSGITPEEETAFLTLKARAVNDGWRRPILRDPGATAAVSKIDYDFHALGVITPVVC
CCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHH
QSSLRAKLLDDRVRAFVTEHPNAVVVDMGAGLDDGYRRIQPPATVDWYSVDLPGMATLRD
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCEEEEEECCCCHHHHHH
EVMPPGPGEHTVGISITDPNWLGGIPSDRPAMVIADGFFAFLTKEQIIATLRAITDHFST
HCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCC
GQLAFNDYGRMVVGVWISKLFPQKMFKKVNQLRGFEGYNDPRTPERWNPRLRFVEEFNLV
CCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEHHHCCCC
DAPEIELFPTWLRISSQLARYSTSMKRSARILRFEF
CCCCCEEEHHHHHHHHHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8509339 [H]