Click here to switch to the map view.

The map label for this gene is hemK [H]

Identifier: 169628534

GI number: 169628534

Start: 1448307

End: 1449149

Strand: Direct

Name: hemK [H]

Synonym: MAB_1443

Alternate gene names: 169628534

Gene position: 1448307-1449149 (Clockwise)

Preceding gene: 169628533

Following gene: 169628535

Centisome position: 28.58

GC content: 65.12

Gene sequence:

>843_bases
ATGAGCCGCTTGCGTGAGCTGCTCGCCGAGGCAGCCGAATCTCTTTCACGGGCAGGTGTTTCCAGCCCTCAGGTGGACGC
CGAGGAGCTGGCCGCCCACCTGCTGGGGGTCTCGCGGACACAGTTGCGCTTTACCGAGGCCGCGGCGGACTTTCCGGGGA
GATATCGAGATCTGGTGGCACAGCGCGCGCGGCGCATTCCGTTGCAGCACTTGGTCGGAAGTGCTGCTTTTGGTCCCATC
GAAGTGCGCGTGGGCCCCGGGGTGTTCATTCCACGTCCTGAAACAGAGTCTCTGTATGCCTGGGCGGCAGGGCAGCTGGC
GCCTCACGCGACGGTTGTCGAGTTATGTGCCGGTTCTGCGGCGCTGGCCATTGCTTTGGCGCAGCACGAGCCGACGGCCC
GTGTCACCGCGATCGAGGTCGACGTGGATGCCCTGATCTACACCCGGCGAAACGCCGAGGGTACCGGCGTCGAGGTGGTC
CAGGCCGATGTGACTTCGCCGGACTTGCTCACCGAGCTGAACGGCGCTGTCGATCTGATCGTCGCGAATCCGCCTTATAT
TCCCCAAGATACTGAGCTGGAGCCCGAAGTGGCGCGGCATGATCCGCCGCAGGCGCTTTTCGCCGGCGCGGACGGGCTCG
CGATCATCGCGCCGATCGTGATCGCCGCCGGAAGGCTCCTGGCGCCGGGGGGCGCCATCGGTATTGAGCATGACGACAGT
AAGGGCGGGCGCACACGCGAGTTGTTTACGGCCTGTGACTTTTTCGATGAGGTTGTGCAGCGGCACGACCTGACCGGACG
GCCACGATTCGTCACCGCTCGCCGGAAAATCGCGGCGTCCTAG

Upstream 100 bases:

>100_bases
TCAAGGCGCATAACCTGGACCAGGTTCTCGATGGAGATCTGGATGCCCTGTTCGATGCGTTGGCAGCGGCCGACCGGAAG
GCGCGCCTGCAGGAGGCGTG

Downstream 100 bases:

>100_bases
ACTGGGCGGGTGACCGTCGTCTACGACTGCCAAGACGCTGCGACCCGCCAGAGCGGGATCGACGCGGCGGTGAGTGCGCT
CAAGGCGGGCGAGCTGGTGG

Product: modification methylase HemK

Products: NA

Alternate protein names: M.MleHemKP [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSRLRELLAEAAESLSRAGVSSPQVDAEELAAHLLGVSRTQLRFTEAAADFPGRYRDLVAQRARRIPLQHLVGSAAFGPI
EVRVGPGVFIPRPETESLYAWAAGQLAPHATVVELCAGSAALAIALAQHEPTARVTAIEVDVDALIYTRRNAEGTGVEVV
QADVTSPDLLTELNGAVDLIVANPPYIPQDTELEPEVARHDPPQALFAGADGLAIIAPIVIAAGRLLAPGGAIGIEHDDS
KGGRTRELFTACDFFDEVVQRHDLTGRPRFVTARRKIAAS

Sequences:

>Translated_280_residues
MSRLRELLAEAAESLSRAGVSSPQVDAEELAAHLLGVSRTQLRFTEAAADFPGRYRDLVAQRARRIPLQHLVGSAAFGPI
EVRVGPGVFIPRPETESLYAWAAGQLAPHATVVELCAGSAALAIALAQHEPTARVTAIEVDVDALIYTRRNAEGTGVEVV
QADVTSPDLLTELNGAVDLIVANPPYIPQDTELEPEVARHDPPQALFAGADGLAIIAPIVIAAGRLLAPGGAIGIEHDDS
KGGRTRELFTACDFFDEVVQRHDLTGRPRFVTARRKIAAS
>Mature_279_residues
SRLRELLAEAAESLSRAGVSSPQVDAEELAAHLLGVSRTQLRFTEAAADFPGRYRDLVAQRARRIPLQHLVGSAAFGPIE
VRVGPGVFIPRPETESLYAWAAGQLAPHATVVELCAGSAALAIALAQHEPTARVTAIEVDVDALIYTRRNAEGTGVEVVQ
ADVTSPDLLTELNGAVDLIVANPPYIPQDTELEPEVARHDPPQALFAGADGLAIIAPIVIAAGRLLAPGGAIGIEHDDSK
GGRTRELFTACDFFDEVVQRHDLTGRPRFVTARRKIAAS

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=298, Percent_Identity=32.5503355704698, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1787463, Length=251, Percent_Identity=32.2709163346614, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082085, Length=176, Percent_Identity=33.5227272727273, Blast_Score=75, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324266, Length=160, Percent_Identity=28.75, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582226, Length=250, Percent_Identity=29.2, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 29780; Mature: 29649

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLRELLAEAAESLSRAGVSSPQVDAEELAAHLLGVSRTQLRFTEAAADFPGRYRDLVA
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHH
QRARRIPLQHLVGSAAFGPIEVRVGPGVFIPRPETESLYAWAAGQLAPHATVVELCAGSA
HHHHCCCHHHHHCCCCCCCEEEEECCCEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCH
ALAIALAQHEPTARVTAIEVDVDALIYTRRNAEGTGVEVVQADVTSPDLLTELNGAVDLI
HEEEEEECCCCCCEEEEEEEEHEEEEEEECCCCCCCEEEEEECCCCCHHHHHCCCCEEEE
VANPPYIPQDTELEPEVARHDPPQALFAGADGLAIIAPIVIAAGRLLAPGGAIGIEHDDS
ECCCCCCCCCCCCCCHHHHCCCCHHHHCCCCCHHHHHHHHHHCCCEECCCCEEECCCCCC
KGGRTRELFTACDFFDEVVQRHDLTGRPRFVTARRKIAAS
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHCCC
>Mature Secondary Structure 
SRLRELLAEAAESLSRAGVSSPQVDAEELAAHLLGVSRTQLRFTEAAADFPGRYRDLVA
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHH
QRARRIPLQHLVGSAAFGPIEVRVGPGVFIPRPETESLYAWAAGQLAPHATVVELCAGSA
HHHHCCCHHHHHCCCCCCCEEEEECCCEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCH
ALAIALAQHEPTARVTAIEVDVDALIYTRRNAEGTGVEVVQADVTSPDLLTELNGAVDLI
HEEEEEECCCCCCEEEEEEEEHEEEEEEECCCCCCCEEEEEECCCCCHHHHHCCCCEEEE
VANPPYIPQDTELEPEVARHDPPQALFAGADGLAIIAPIVIAAGRLLAPGGAIGIEHDDS
ECCCCCCCCCCCCCCHHHHCCCCHHHHCCCCCHHHHHHHHHHCCCEECCCCEEECCCCCC
KGGRTRELFTACDFFDEVVQRHDLTGRPRFVTARRKIAAS
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]