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The map label for this gene is garR [C]

Identifier: 169628313

GI number: 169628313

Start: 1232437

End: 1233303

Strand: Direct

Name: garR [C]

Synonym: MAB_1220

Alternate gene names: 169628313

Gene position: 1232437-1233303 (Clockwise)

Preceding gene: 169628312

Following gene: 169628314

Centisome position: 24.32

GC content: 66.32

Gene sequence:

>867_bases
ATGACGCAGCAAGCCATGAAACTGGGATACATCGGCCTGGGCAATATGGGGGCGCCCATGGCCCGGCGGCTTGTCGATTG
GCCAGGCGGCCTCACGGTCTTCGACCTGCGGGCCGAGGCGGTGGCGCCGCTGGTTGAAGAAGGCGCCGTGGGTGCCTCCG
ATGTGGCCGATATCGCGGATGCCGACCTCATCAGTGTCACCGTGCTCGATGACGCACAGGTGCGTCAGGTAGTGGGGCAG
GTCGCCCCGAAGGCAAAGCCCGGCACCATCATCGCGATCCATTCCACCATTAGCGATACGACGGCCGTCGAGCTGGCGCA
GGCCTGGAGGCCGCGCGATATTCACGTCATCGACGCACCGGTGAGCGGCGGTGCGCCGGGTGCGCACAAGGGTGAGCTGG
CCACCATGGTGGGTGCCGACGGCGAGACCTTCGCGCGGGTCAAAGAGGCCTTCTCGAAGTGGGCGTCGTTGGTGATTCAT
GCCGGTGCCCCGGGCGCAGGCACCCGAATGAAGCTGGCGCGCAACCTTCTACATTTTGTGGCCTTCACGGCCGCGGGGGA
GGCGCAGCGCTTGGCCGAGAGCTGTGGATTGGACATCACCGACCTGGGCAAGGTCGTGCGGCACACCGATGCCATCACCG
GTGGGCCCGGTGCCATCATGCTGCGCGACACCACCGAGCCGGTGACGCCGGACAGTTTCTGGCTGCCCATCTTCACCCAT
GTACGGGGCCTGGGCGAGAAGGACCTGAGCCTGGCGCTGGCGCTGGGCGAGGAGCAATCCGTCGACTTGCCGTTGGCGAC
GCTGGCCCGTGAGCAGCTGGCGGCGAGTCTTGGTGTGCCACACGAGGAAGGGGCAAGTAATGGATGA

Upstream 100 bases:

>100_bases
TTGACGACATGGTGGGCATGTGTCTTTTCCTGTTGTCGGATAAGGCGTCGTGGATCACCGGCCAGGTATTCAACGTCGAT
GGCGGACAGATCATCCGGTC

Downstream 100 bases:

>100_bases
GTTGCGCCGCCAGGGTCTGGACAAGATGAAAGAGGTGTACGGCTGGGACATGCCCGACATCCCTGGTGACTACTTCAAAT
ACACCATCGATCATCTCTTC

Product: dehydrogenase/reductase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MTQQAMKLGYIGLGNMGAPMARRLVDWPGGLTVFDLRAEAVAPLVEEGAVGASDVADIADADLISVTVLDDAQVRQVVGQ
VAPKAKPGTIIAIHSTISDTTAVELAQAWRPRDIHVIDAPVSGGAPGAHKGELATMVGADGETFARVKEAFSKWASLVIH
AGAPGAGTRMKLARNLLHFVAFTAAGEAQRLAESCGLDITDLGKVVRHTDAITGGPGAIMLRDTTEPVTPDSFWLPIFTH
VRGLGEKDLSLALALGEEQSVDLPLATLAREQLAASLGVPHEEGASNG

Sequences:

>Translated_288_residues
MTQQAMKLGYIGLGNMGAPMARRLVDWPGGLTVFDLRAEAVAPLVEEGAVGASDVADIADADLISVTVLDDAQVRQVVGQ
VAPKAKPGTIIAIHSTISDTTAVELAQAWRPRDIHVIDAPVSGGAPGAHKGELATMVGADGETFARVKEAFSKWASLVIH
AGAPGAGTRMKLARNLLHFVAFTAAGEAQRLAESCGLDITDLGKVVRHTDAITGGPGAIMLRDTTEPVTPDSFWLPIFTH
VRGLGEKDLSLALALGEEQSVDLPLATLAREQLAASLGVPHEEGASNG
>Mature_287_residues
TQQAMKLGYIGLGNMGAPMARRLVDWPGGLTVFDLRAEAVAPLVEEGAVGASDVADIADADLISVTVLDDAQVRQVVGQV
APKAKPGTIIAIHSTISDTTAVELAQAWRPRDIHVIDAPVSGGAPGAHKGELATMVGADGETFARVKEAFSKWASLVIHA
GAPGAGTRMKLARNLLHFVAFTAAGEAQRLAESCGLDITDLGKVVRHTDAITGGPGAIMLRDTTEPVTPDSFWLPIFTHV
RGLGEKDLSLALALGEEQSVDLPLATLAREQLAASLGVPHEEGASNG

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=254, Percent_Identity=31.1023622047244, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI40556376, Length=275, Percent_Identity=25.4545454545455, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI145693186, Length=277, Percent_Identity=33.5740072202166, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790315, Length=273, Percent_Identity=29.3040293040293, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1789092, Length=184, Percent_Identity=38.5869565217391, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786719, Length=275, Percent_Identity=30.5454545454545, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17557316, Length=273, Percent_Identity=33.3333333333333, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24655230, Length=274, Percent_Identity=29.1970802919708, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19922568, Length=274, Percent_Identity=29.1970802919708, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 29913; Mature: 29782

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQAMKLGYIGLGNMGAPMARRLVDWPGGLTVFDLRAEAVAPLVEEGAVGASDVADIAD
CCCHHHHCCCEECCCCCCHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCCHHHHHHCC
ADLISVTVLDDAQVRQVVGQVAPKAKPGTIIAIHSTISDTTAVELAQAWRPRDIHVIDAP
CCEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECC
VSGGAPGAHKGELATMVGADGETFARVKEAFSKWASLVIHAGAPGAGTRMKLARNLLHFV
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHH
AFTAAGEAQRLAESCGLDITDLGKVVRHTDAITGGPGAIMLRDTTEPVTPDSFWLPIFTH
HHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHH
VRGLGEKDLSLALALGEEQSVDLPLATLAREQLAASLGVPHEEGASNG
HHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TQQAMKLGYIGLGNMGAPMARRLVDWPGGLTVFDLRAEAVAPLVEEGAVGASDVADIAD
CCHHHHCCCEECCCCCCHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCCHHHHHHCC
ADLISVTVLDDAQVRQVVGQVAPKAKPGTIIAIHSTISDTTAVELAQAWRPRDIHVIDAP
CCEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECC
VSGGAPGAHKGELATMVGADGETFARVKEAFSKWASLVIHAGAPGAGTRMKLARNLLHFV
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHH
AFTAAGEAQRLAESCGLDITDLGKVVRHTDAITGGPGAIMLRDTTEPVTPDSFWLPIFTH
HHHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHH
VRGLGEKDLSLALALGEEQSVDLPLATLAREQLAASLGVPHEEGASNG
HHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]