Click here to switch to the map view.

The map label for this gene is purT

Identifier: 169628284

GI number: 169628284

Start: 1204802

End: 1206010

Strand: Reverse

Name: purT

Synonym: MAB_1191c

Alternate gene names: 169628284

Gene position: 1206010-1204802 (Counterclockwise)

Preceding gene: 169628285

Following gene: 169628282

Centisome position: 23.8

GC content: 67.25

Gene sequence:

>1209_bases
ATGACCACTATCGGAACTCCCCTGTCACCGCGCGCCACCCGTGTCATGCTGCTCGGTTCCGGCGAACTCGGCCGCGAGGT
CCTGATCGCCCTGCAGCGTCTGGGCGTCGAGACCATCGCCGTCGATCGCTACGAGAACGCCCCCGGCCACCAGATCGCCC
ACCACGCGCGCGTCATCTCGATGACCGACCCCGAACAGTTGCGCGCCCTGATCGAGGCCGAGAAGCCCGACCTGGTGGTG
CCCGAGATCGAGGCCATCGCCACGGCCACCCTGGAAGCCCTGGAGACCGAGGGCGTGGTACGCGTGATCCCGACAGCCCG
CGCGGCGCGGCTCACCATGGACCGCGAGGGTATCCGCCGGCTCGCGGCGGAGACACTTGGATTGCCCACCAGCCCTTACC
AATTCTGCGGCTCACTCGATGAGCTCCAGGCGGCGATCGACCCGGAGTCCGGGGGCGTCGGCTACCCCTGCGTGGTGAAG
CCGCTCATGAGCAGCTCCGGCAAGGGGCAGAGCAAGCTCGACGGACCGGCCGATGTCGAGAAGGCCTGGGACTACGCGAT
GGCGGGTAGCCGGGTCACCAACAACCGCGTGATCGTCGAGGGGTTTGTCGACTTCGACTACGAGATCACGCTTTTGACGG
TGCGGGCGCTAGGCGACGACGGCGAAGTCGAGACCAGCTTCTGCGCGCCCATCGGGCACCGGCAGGCCAATGGTGACTAT
GTCGAGAGTTGGCAGCCGCATCCCATGACGGAGGCCGCCTTGCGCAGCGCGCAGCACATCGCCCGATCCGTTACCGAGAA
CCTGGGCGGCCAGGGAATTTTCGGTGTCGAGCTGTTCATCAAGGGCGATCAGGTGTATTTCAGCGAAGTGAGTCCGCGCC
CCCACGACACCGGCATGGTCACCATGATCACGCAGTGGCAGAACGAGTTCGAGTTGCATGCCCGCGCCATTCTCGGGCTT
CCCGTCGATACGACGTTGAAATCTGCGGGGGCCAGCGCGGTGATCTATGGCGGGGTCGAGGCGCAAGGCGTGGTGTTCGA
TGGTGTCGACGAGGCGCTCCGCGTCCCGCGGACCGAGCTGCGGCTGTTCGGCAAGCCGGAAAGCTTCATCACCCGCCGGA
TGGGCGTGGCGCTGGCCCGCGACGAGGATGTGGACACGGCCCGGCGCAACGCCGCCGAGGCCGCGGGCCGGGTCCGCGTT
TCCGGCTGA

Upstream 100 bases:

>100_bases
GCGGGTTCGTATCGCACGTTCCCGCCACGACACGGCGTGTCGGCGTTTGGGGCGCCTGCCGGCCGGGCGGCAGGCGCCCG
CTCACTAGACTTCCGTCGCG

Downstream 100 bases:

>100_bases
ACATTCGCATTAAAAAGGGGCGCCCCTTCCGGAGCGCCCCTTTTCGTTGCTTAGTGCTAGTCGTTCTGGAAGTAGCTCAG
CAGTCGCAGGATTTCGACGT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTTIGTPLSPRATRVMLLGSGELGREVLIALQRLGVETIAVDRYENAPGHQIAHHARVISMTDPEQLRALIEAEKPDLVV
PEIEAIATATLEALETEGVVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYQFCGSLDELQAAIDPESGGVGYPCVVK
PLMSSSGKGQSKLDGPADVEKAWDYAMAGSRVTNNRVIVEGFVDFDYEITLLTVRALGDDGEVETSFCAPIGHRQANGDY
VESWQPHPMTEAALRSAQHIARSVTENLGGQGIFGVELFIKGDQVYFSEVSPRPHDTGMVTMITQWQNEFELHARAILGL
PVDTTLKSAGASAVIYGGVEAQGVVFDGVDEALRVPRTELRLFGKPESFITRRMGVALARDEDVDTARRNAAEAAGRVRV
SG

Sequences:

>Translated_402_residues
MTTIGTPLSPRATRVMLLGSGELGREVLIALQRLGVETIAVDRYENAPGHQIAHHARVISMTDPEQLRALIEAEKPDLVV
PEIEAIATATLEALETEGVVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYQFCGSLDELQAAIDPESGGVGYPCVVK
PLMSSSGKGQSKLDGPADVEKAWDYAMAGSRVTNNRVIVEGFVDFDYEITLLTVRALGDDGEVETSFCAPIGHRQANGDY
VESWQPHPMTEAALRSAQHIARSVTENLGGQGIFGVELFIKGDQVYFSEVSPRPHDTGMVTMITQWQNEFELHARAILGL
PVDTTLKSAGASAVIYGGVEAQGVVFDGVDEALRVPRTELRLFGKPESFITRRMGVALARDEDVDTARRNAAEAAGRVRV
SG
>Mature_401_residues
TTIGTPLSPRATRVMLLGSGELGREVLIALQRLGVETIAVDRYENAPGHQIAHHARVISMTDPEQLRALIEAEKPDLVVP
EIEAIATATLEALETEGVVRVIPTARAARLTMDREGIRRLAAETLGLPTSPYQFCGSLDELQAAIDPESGGVGYPCVVKP
LMSSSGKGQSKLDGPADVEKAWDYAMAGSRVTNNRVIVEGFVDFDYEITLLTVRALGDDGEVETSFCAPIGHRQANGDYV
ESWQPHPMTEAALRSAQHIARSVTENLGGQGIFGVELFIKGDQVYFSEVSPRPHDTGMVTMITQWQNEFELHARAILGLP
VDTTLKSAGASAVIYGGVEAQGVVFDGVDEALRVPRTELRLFGKPESFITRRMGVALARDEDVDTARRNAAEAAGRVRVS
G

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=403, Percent_Identity=57.0719602977667, Blast_Score=418, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6324702, Length=377, Percent_Identity=25.7294429708223, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_MYCA9 (B1MKI7)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001701933.1
- ProteinModelPortal:   B1MKI7
- SMR:   B1MKI7
- EnsemblBacteria:   EBMYCT00000004672
- GeneID:   5963715
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_1191c
- GeneTree:   EBGT00050000015722
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- ProtClustDB:   PRK09288
- BioCyc:   MABS561007:MAB_1191C-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 43387; Mature: 43256

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 82-82 BINDING 115-115 BINDING 160-160 BINDING 208-208 BINDING 277-277 BINDING 289-289 BINDING 296-296 BINDING 365-365

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIGTPLSPRATRVMLLGSGELGREVLIALQRLGVETIAVDRYENAPGHQIAHHARVIS
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHEEEEE
MTDPEQLRALIEAEKPDLVVPEIEAIATATLEALETEGVVRVIPTARAARLTMDREGIRR
CCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCEEEEECCCCHHHEECCHHHHHH
LAAETLGLPTSPYQFCGSLDELQAAIDPESGGVGYPCVVKPLMSSSGKGQSKLDGPADVE
HHHHHHCCCCCHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHH
KAWDYAMAGSRVTNNRVIVEGFVDFDYEITLLTVRALGDDGEVETSFCAPIGHRQANGDY
HHHHHHHCCCEEECCEEEEEEEECCCEEEEEEEEEECCCCCCCCHHHCCCCCCCCCCCCH
VESWQPHPMTEAALRSAQHIARSVTENLGGQGIFGVELFIKGDQVYFSEVSPRPHDTGMV
HCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEE
TMITQWQNEFELHARAILGLPVDTTLKSAGASAVIYGGVEAQGVVFDGVDEALRVPRTEL
EEEEHHCCHHHEEEHEEECCCCCHHHHHCCCCEEEECCCCCCCEEECCCHHHHHCCHHHH
RLFGKPESFITRRMGVALARDEDVDTARRNAAEAAGRVRVSG
EECCCCHHHHHHHHCEEEECCCCHHHHHHHHHHHCCEEEECC
>Mature Secondary Structure 
TTIGTPLSPRATRVMLLGSGELGREVLIALQRLGVETIAVDRYENAPGHQIAHHARVIS
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHEEEEE
MTDPEQLRALIEAEKPDLVVPEIEAIATATLEALETEGVVRVIPTARAARLTMDREGIRR
CCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHCCCEEEEECCCCHHHEECCHHHHHH
LAAETLGLPTSPYQFCGSLDELQAAIDPESGGVGYPCVVKPLMSSSGKGQSKLDGPADVE
HHHHHHCCCCCHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHH
KAWDYAMAGSRVTNNRVIVEGFVDFDYEITLLTVRALGDDGEVETSFCAPIGHRQANGDY
HHHHHHHCCCEEECCEEEEEEEECCCEEEEEEEEEECCCCCCCCHHHCCCCCCCCCCCCH
VESWQPHPMTEAALRSAQHIARSVTENLGGQGIFGVELFIKGDQVYFSEVSPRPHDTGMV
HCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEE
TMITQWQNEFELHARAILGLPVDTTLKSAGASAVIYGGVEAQGVVFDGVDEALRVPRTEL
EEEEHHCCHHHEEEHEEECCCCCHHHHHCCCCEEEECCCCCCCEEECCCHHHHHCCHHHH
RLFGKPESFITRRMGVALARDEDVDTARRNAAEAAGRVRVSG
EECCCCHHHHHHHHCEEEECCCCHHHHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA