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The map label for this gene is crt [H]

Identifier: 169628280

GI number: 169628280

Start: 1199893

End: 1200924

Strand: Reverse

Name: crt [H]

Synonym: MAB_1187c

Alternate gene names: 169628280

Gene position: 1200924-1199893 (Counterclockwise)

Preceding gene: 169628281

Following gene: 169628279

Centisome position: 23.7

GC content: 65.7

Gene sequence:

>1032_bases
GTGACGGATCAGATCGAAACCCGGGTCGAGAAGGGGGTCGGCCTGCTGACGCTGAACCGTCCCAAGGCCATCAACTCGCT
CACCTACGACATGATCGCGGCGATGAACGCCGTCCTGGCGGAGTGGGAGGCGGACGACGCGGTACGCGTGGTCGTGCTCT
CGGGCGCCGGCGAGCGTGGTCTGTGCGCCGGCGGCGACGTGGTGGCCGTGCACGACAGCGCCAAGATCGACGGCGTGGCA
GCCCGCAGGTTCTTCCGCGACGAGTACGTGCTCAACGCACAGATCGCGCGCTTCCCGAAACCGTATGTGGCGCTGATGGA
CGGCATCGTGATGGGCGGCGGTGTCGGAGTCGGCGGGCATGCCAACATCCGCATTGTCACCGATACCTCCAAGGTGGCGA
TGCCGGAGGTGGGCATCGGCTTCATCCCGGACGTGGGCGGTACCTACCTACTCTCGCGCGCCCCCGGCCAGCTCGGCTTG
CACGCCGCACTCACGGGTGCACCCTTCTCCGGAGCCGACGCCATCGCGCTGGGCTTCGCCGACCACTACGTGCCGCATGA
CCGGCTCGAGAAGTTCACCAAGGCCATCATCGATAGCGATATCGCCACTGCGCTTGCGGTCCACGGCACCAACGCACCGT
CGAGTGATCTGCTGGCTCAACGCAATTGGATCGACGAGTGCTACGGCGGTGAATCCGTCGCCGATATCGTCGCCGCGCTG
CAGGCCCGTGGTGACGAACGGGCACGCGCGGCAGCCGATCAAATCCTGACCCGCTCCCCCATCGCACTGTCGGTGACGCT
TGCGGCAGTGCGCAGGGCTCGCGAACTGCCGTCGCTGGAAGCGGTTCTCACCCAGGAATATCGAGTGTCCAGCCAATCGG
TGCGCTCGCATGATCTGGTCGAGGGCATCAGGGCTCAGCTGGTCGACAAGGATCGCAACCCGCAGTGGGTTCCGAAGACA
CTCGCCGAGGTCACCAAGGCCGATGTCGAGGAGTTCTTCCAGCCAGTCAGCGACGACTTGGAGTGGCCGTGA

Upstream 100 bases:

>100_bases
AGATCGTGCGTGACCTACGGGTACACCAAATCCTGGAAGGAACCAACGAGATCATGCGAGTCATCGTGTCCCGCAAGTTG
ATAGAACAGGGGCTTCCGGC

Downstream 100 bases:

>100_bases
CCAATATCGCCTTTATCGGCCTGGGCCACATGGGATTACCGATGGCGGTCAATCTCACCAAGGCCGGACACACCGTGGCG
GCTTTCGATCTCTCCGAGCA

Product: 3-hydroxyisobutyryl-CoA hydrolase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MTDQIETRVEKGVGLLTLNRPKAINSLTYDMIAAMNAVLAEWEADDAVRVVVLSGAGERGLCAGGDVVAVHDSAKIDGVA
ARRFFRDEYVLNAQIARFPKPYVALMDGIVMGGGVGVGGHANIRIVTDTSKVAMPEVGIGFIPDVGGTYLLSRAPGQLGL
HAALTGAPFSGADAIALGFADHYVPHDRLEKFTKAIIDSDIATALAVHGTNAPSSDLLAQRNWIDECYGGESVADIVAAL
QARGDERARAAADQILTRSPIALSVTLAAVRRARELPSLEAVLTQEYRVSSQSVRSHDLVEGIRAQLVDKDRNPQWVPKT
LAEVTKADVEEFFQPVSDDLEWP

Sequences:

>Translated_343_residues
MTDQIETRVEKGVGLLTLNRPKAINSLTYDMIAAMNAVLAEWEADDAVRVVVLSGAGERGLCAGGDVVAVHDSAKIDGVA
ARRFFRDEYVLNAQIARFPKPYVALMDGIVMGGGVGVGGHANIRIVTDTSKVAMPEVGIGFIPDVGGTYLLSRAPGQLGL
HAALTGAPFSGADAIALGFADHYVPHDRLEKFTKAIIDSDIATALAVHGTNAPSSDLLAQRNWIDECYGGESVADIVAAL
QARGDERARAAADQILTRSPIALSVTLAAVRRARELPSLEAVLTQEYRVSSQSVRSHDLVEGIRAQLVDKDRNPQWVPKT
LAEVTKADVEEFFQPVSDDLEWP
>Mature_342_residues
TDQIETRVEKGVGLLTLNRPKAINSLTYDMIAAMNAVLAEWEADDAVRVVVLSGAGERGLCAGGDVVAVHDSAKIDGVAA
RRFFRDEYVLNAQIARFPKPYVALMDGIVMGGGVGVGGHANIRIVTDTSKVAMPEVGIGFIPDVGGTYLLSRAPGQLGLH
AALTGAPFSGADAIALGFADHYVPHDRLEKFTKAIIDSDIATALAVHGTNAPSSDLLAQRNWIDECYGGESVADIVAALQ
ARGDERARAAADQILTRSPIALSVTLAAVRRARELPSLEAVLTQEYRVSSQSVRSHDLVEGIRAQLVDKDRNPQWVPKTL
AEVTKADVEEFFQPVSDDLEWP

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=342, Percent_Identity=37.719298245614, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI37594469, Length=289, Percent_Identity=36.6782006920415, Blast_Score=194, Evalue=9e-50,
Organism=Homo sapiens, GI194097323, Length=206, Percent_Identity=32.0388349514563, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787660, Length=224, Percent_Identity=34.375, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1787659, Length=188, Percent_Identity=28.1914893617021, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788682, Length=181, Percent_Identity=27.6243093922652, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI221142681, Length=175, Percent_Identity=27.4285714285714, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788597, Length=199, Percent_Identity=26.6331658291457, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=337, Percent_Identity=37.3887240356083, Blast_Score=234, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI25144157, Length=337, Percent_Identity=37.3887240356083, Blast_Score=234, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17540714, Length=228, Percent_Identity=28.9473684210526, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=319, Percent_Identity=24.4514106583072, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=181, Percent_Identity=27.6243093922652, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=196, Percent_Identity=30.6122448979592, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=351, Percent_Identity=33.6182336182336, Blast_Score=176, Evalue=5e-45,
Organism=Drosophila melanogaster, GI28571730, Length=333, Percent_Identity=36.3363363363363, Blast_Score=225, Evalue=4e-59,
Organism=Drosophila melanogaster, GI28571729, Length=333, Percent_Identity=36.3363363363363, Blast_Score=224, Evalue=5e-59,
Organism=Drosophila melanogaster, GI20129971, Length=183, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24653477, Length=183, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24653139, Length=179, Percent_Identity=31.2849162011173, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19922422, Length=209, Percent_Identity=28.2296650717703, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 36663; Mature: 36532

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQIETRVEKGVGLLTLNRPKAINSLTYDMIAAMNAVLAEWEADDAVRVVVLSGAGERG
CCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
LCAGGDVVAVHDSAKIDGVAARRFFRDEYVLNAQIARFPKPYVALMDGIVMGGGVGVGGH
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCCCCCCC
ANIRIVTDTSKVAMPEVGIGFIPDVGGTYLLSRAPGQLGLHAALTGAPFSGADAIALGFA
CEEEEEECCCHHCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCCEEEECCH
DHYVPHDRLEKFTKAIIDSDIATALAVHGTNAPSSDLLAQRNWIDECYGGESVADIVAAL
HHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
QARGDERARAAADQILTRSPIALSVTLAAVRRARELPSLEAVLTQEYRVSSQSVRSHDLV
HHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
EGIRAQLVDKDRNPQWVPKTLAEVTKADVEEFFQPVSDDLEWP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDQIETRVEKGVGLLTLNRPKAINSLTYDMIAAMNAVLAEWEADDAVRVVVLSGAGERG
CCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
LCAGGDVVAVHDSAKIDGVAARRFFRDEYVLNAQIARFPKPYVALMDGIVMGGGVGVGGH
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCCCCCCC
ANIRIVTDTSKVAMPEVGIGFIPDVGGTYLLSRAPGQLGLHAALTGAPFSGADAIALGFA
CEEEEEECCCHHCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCCCEEEECCH
DHYVPHDRLEKFTKAIIDSDIATALAVHGTNAPSSDLLAQRNWIDECYGGESVADIVAAL
HHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
QARGDERARAAADQILTRSPIALSVTLAAVRRARELPSLEAVLTQEYRVSSQSVRSHDLV
HHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
EGIRAQLVDKDRNPQWVPKTLAEVTKADVEEFFQPVSDDLEWP
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]