Click here to switch to the map view.

The map label for this gene is mmsB [H]

Identifier: 169628279

GI number: 169628279

Start: 1199015

End: 1199896

Strand: Reverse

Name: mmsB [H]

Synonym: MAB_1186c

Alternate gene names: 169628279

Gene position: 1199896-1199015 (Counterclockwise)

Preceding gene: 169628280

Following gene: 169628278

Centisome position: 23.68

GC content: 67.46

Gene sequence:

>882_bases
GTGACCAATATCGCCTTTATCGGCCTGGGCCACATGGGATTACCGATGGCGGTCAATCTCACCAAGGCCGGACACACCGT
GGCGGCTTTCGATCTCTCCGAGCAGGCCCGGGACGCCGCCACCCAGGCCGGTGTGCCGTTGGCCGAGTCGGGCGCCGCGG
CTGCCAGCACCGCCGATGTGGTGATCACCATGCTGCCCAAGGGCGCGCACGTGCTGGCGGCCTACGAGGATCTGCTGCCC
GCCGCGCGTCCCGGCACCCTGTTCATCGATTCCTCCACCGTCGACGTGGCCGATGCCCGCGCCGCGCATGAGGCTGCGAC
CGCCGCGGGACACCGCTTCGCCGACGCCCCCGTGTCCGGCGGGGTGCCCGGGGCCACCGCGGCGACTCTGACGTTCATGG
TGGGTGCCGACGACGCCGTATTCGTAGCCGCAGAACCGATTCTGGATGCCATGGGCAAGCGCGTGGTGCACTGCGGTGGT
CCCGGCGTCGGGCAGGCCGCGAAGATCTGCAACAACATGATCCTCGGCGTATCGATGATCGCCATCAGCGAGGCGTTTGT
GCTCGGCGAGAAACTCGGGTTGTCCCATCAGGCCCTGTTCGATGTTGCGGCCAACGCCTCGGGCCAGTGTTGGGCGCTCA
CCTCGAACTGTCCGGTGCCCGGCCCAGTGCCCACCAGCCCTGCCAATCGCGACTACGCCCCCGGCTTCGCCGTCGCGTTG
ATGGCCAAGGACTTGGGGCTGGCGGCCAATGCGGTGCGCGCCAATGGAGTTGACGCACAGCTGGGCCTGGCCGCGGCACA
CATCTACGACGACTTCAATTCCACCGGAGGCTCCGGCCTAGACTTCTCGGCGATCTTCTCGCGAATCCAAGGAGCGTCAT
GA

Upstream 100 bases:

>100_bases
AGGATCGCAACCCGCAGTGGGTTCCGAAGACACTCGCCGAGGTCACCAAGGCCGATGTCGAGGAGTTCTTCCAGCCAGTC
AGCGACGACTTGGAGTGGCC

Downstream 100 bases:

>100_bases
CCTCACACAATTTCGAAACCATCCTCACCGAGCGCATCGACCGTGTCGCGGTCATCACGCTGAACCGGCCCAAGGCGCTC
AACGCACTGAATTCGCAGGT

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTNIAFIGLGHMGLPMAVNLTKAGHTVAAFDLSEQARDAATQAGVPLAESGAAAASTADVVITMLPKGAHVLAAYEDLLP
AARPGTLFIDSSTVDVADARAAHEAATAAGHRFADAPVSGGVPGATAATLTFMVGADDAVFVAAEPILDAMGKRVVHCGG
PGVGQAAKICNNMILGVSMIAISEAFVLGEKLGLSHQALFDVAANASGQCWALTSNCPVPGPVPTSPANRDYAPGFAVAL
MAKDLGLAANAVRANGVDAQLGLAAAHIYDDFNSTGGSGLDFSAIFSRIQGAS

Sequences:

>Translated_293_residues
MTNIAFIGLGHMGLPMAVNLTKAGHTVAAFDLSEQARDAATQAGVPLAESGAAAASTADVVITMLPKGAHVLAAYEDLLP
AARPGTLFIDSSTVDVADARAAHEAATAAGHRFADAPVSGGVPGATAATLTFMVGADDAVFVAAEPILDAMGKRVVHCGG
PGVGQAAKICNNMILGVSMIAISEAFVLGEKLGLSHQALFDVAANASGQCWALTSNCPVPGPVPTSPANRDYAPGFAVAL
MAKDLGLAANAVRANGVDAQLGLAAAHIYDDFNSTGGSGLDFSAIFSRIQGAS
>Mature_292_residues
TNIAFIGLGHMGLPMAVNLTKAGHTVAAFDLSEQARDAATQAGVPLAESGAAAASTADVVITMLPKGAHVLAAYEDLLPA
ARPGTLFIDSSTVDVADARAAHEAATAAGHRFADAPVSGGVPGATAATLTFMVGADDAVFVAAEPILDAMGKRVVHCGGP
GVGQAAKICNNMILGVSMIAISEAFVLGEKLGLSHQALFDVAANASGQCWALTSNCPVPGPVPTSPANRDYAPGFAVALM
AKDLGLAANAVRANGVDAQLGLAAAHIYDDFNSTGGSGLDFSAIFSRIQGAS

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=42.1232876712329, Blast_Score=215, Evalue=4e-56,
Organism=Homo sapiens, GI40556376, Length=289, Percent_Identity=29.0657439446367, Blast_Score=101, Evalue=8e-22,
Organism=Escherichia coli, GI145693186, Length=268, Percent_Identity=37.3134328358209, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1786719, Length=295, Percent_Identity=29.8305084745763, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1790315, Length=298, Percent_Identity=33.2214765100671, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789092, Length=294, Percent_Identity=28.2312925170068, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17557316, Length=273, Percent_Identity=42.4908424908425, Blast_Score=200, Evalue=9e-52,
Organism=Drosophila melanogaster, GI24655230, Length=289, Percent_Identity=44.2906574394464, Blast_Score=244, Evalue=4e-65,
Organism=Drosophila melanogaster, GI19922568, Length=289, Percent_Identity=44.2906574394464, Blast_Score=244, Evalue=4e-65,
Organism=Drosophila melanogaster, GI28574115, Length=295, Percent_Identity=23.728813559322, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 29264; Mature: 29133

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIAFIGLGHMGLPMAVNLTKAGHTVAAFDLSEQARDAATQAGVPLAESGAAAASTADV
CCEEEEEEECCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCEE
VITMLPKGAHVLAAYEDLLPAARPGTLFIDSSTVDVADARAAHEAATAAGHRFADAPVSG
EEEECCCCCEEEHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
GVPGATAATLTFMVGADDAVFVAAEPILDAMGKRVVHCGGPGVGQAAKICNNMILGVSMI
CCCCCCCEEEEEEECCCCEEEEEHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHH
AISEAFVLGEKLGLSHQALFDVAANASGQCWALTSNCPVPGPVPTSPANRDYAPGFAVAL
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
MAKDLGLAANAVRANGVDAQLGLAAAHIYDDFNSTGGSGLDFSAIFSRIQGAS
HHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TNIAFIGLGHMGLPMAVNLTKAGHTVAAFDLSEQARDAATQAGVPLAESGAAAASTADV
CEEEEEEECCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCEE
VITMLPKGAHVLAAYEDLLPAARPGTLFIDSSTVDVADARAAHEAATAAGHRFADAPVSG
EEEECCCCCEEEHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
GVPGATAATLTFMVGADDAVFVAAEPILDAMGKRVVHCGGPGVGQAAKICNNMILGVSMI
CCCCCCCEEEEEEECCCCEEEEEHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHH
AISEAFVLGEKLGLSHQALFDVAANASGQCWALTSNCPVPGPVPTSPANRDYAPGFAVAL
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
MAKDLGLAANAVRANGVDAQLGLAAAHIYDDFNSTGGSGLDFSAIFSRIQGAS
HHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]