BacMap

Definition	Mycobacterium abscessus ATCC 19977 chromosome chromosome 1, complete sequence.
Accession	NC_010397
Length	5,067,172

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The map label for this gene is echA8 [H]

Identifier: 169628278

GI number: 169628278

Start: 1198236

End: 1199018

Strand: Reverse

Name: echA8 [H]

Synonym: MAB_1185c

Alternate gene names: 169628278

Gene position: 1199018-1198236 (Counterclockwise)

Preceding gene: 169628279

Following gene: 169628277

Centisome position: 23.66

GC content: 64.11

Gene sequence:

>783_bases
ATGACCTCACACAATTTCGAAACCATCCTCACCGAGCGCATCGACCGTGTCGCGGTCATCACGCTGAACCGGCCCAAGGC
GCTCAACGCACTGAATTCGCAGGTGATGAACGAGGTCACCACCGCCGCAGCCGAATTCGACGCCGATCACGGTATCGGCG
CCATCATCATCACCGGTAGCGAGAAGGCCTTCGCGGCGGGCGCGGATATCAAGGAGATGAGCGAGCAGTCGTTCAGTGAC
ATGTTCGGTTCCGACTTCTTTTCCGCCTGGGGCAAGCTGGGCGCGGTGCGCACCCCGACCATCGCGGCGGTGAGCGGATA
CGCCCTCGGTGGTGGATGCGAGCTGGCCATGATGTGCGACGTGATCATCGCCGCCGAGAACGCCACGTTCGGCCAACCCG
AGATCAAACTGGGCGTGCTGCCCGGCATGGGCGGCTCCCAGCGGCTCACCCGCGCCATTGGCAAGGCGAAGGCCATGGAC
ATGATCCTCACCGGCCGCAATATGGACGCCGCCGAAGCCGAGCGCAGCGGCCTGGTCTCGCGCGTGGTCGCCACCGAGAG
CCTGCTCGACGAGGCCAAGGCCGTCGCCAAGACCATCTCGGAGATGTCACTGTCGGCTTCGATGATGGCCAAGGAAGCCG
TGAACCGGGCCTTCGAATCCAGCCTGGCCGAGGGATTGTTGTTCGAGCGCAGGATCTTCCACTCGGCATTCGGTACCGCC
GACCAGTCCGAGGGCATGGCCGCGTTCGTCGAGAAGCGCCCCGCCAACTTCATTCACCGCTGA

Upstream 100 bases:

>100_bases
AGCTGGGCCTGGCCGCGGCACACATCTACGACGACTTCAATTCCACCGGAGGCTCCGGCCTAGACTTCTCGGCGATCTTC
TCGCGAATCCAAGGAGCGTC

Downstream 100 bases:

>100_bases
GCGCATGACCCTGCCGTTCGATCCCGTCGACGATGCCCATCGCCATTGGGTGGAAAACGGCTGGGGCGAGGTCGCCGACG
GGATGGCCGCCGTCACGTCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTSHNFETILTERIDRVAVITLNRPKALNALNSQVMNEVTTAAAEFDADHGIGAIIITGSEKAFAAGADIKEMSEQSFSD
MFGSDFFSAWGKLGAVRTPTIAAVSGYALGGGCELAMMCDVIIAAENATFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMD
MILTGRNMDAAEAERSGLVSRVVATESLLDEAKAVAKTISEMSLSASMMAKEAVNRAFESSLAEGLLFERRIFHSAFGTA
DQSEGMAAFVEKRPANFIHR

Sequences:

>Translated_260_residues
MTSHNFETILTERIDRVAVITLNRPKALNALNSQVMNEVTTAAAEFDADHGIGAIIITGSEKAFAAGADIKEMSEQSFSD
MFGSDFFSAWGKLGAVRTPTIAAVSGYALGGGCELAMMCDVIIAAENATFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMD
MILTGRNMDAAEAERSGLVSRVVATESLLDEAKAVAKTISEMSLSASMMAKEAVNRAFESSLAEGLLFERRIFHSAFGTA
DQSEGMAAFVEKRPANFIHR
>Mature_259_residues
TSHNFETILTERIDRVAVITLNRPKALNALNSQVMNEVTTAAAEFDADHGIGAIIITGSEKAFAAGADIKEMSEQSFSDM
FGSDFFSAWGKLGAVRTPTIAAVSGYALGGGCELAMMCDVIIAAENATFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDM
ILTGRNMDAAEAERSGLVSRVVATESLLDEAKAVAKTISEMSLSASMMAKEAVNRAFESSLAEGLLFERRIFHSAFGTAD
QSEGMAAFVEKRPANFIHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=53.125, Blast_Score=278, Evalue=3e-75,
Organism=Homo sapiens, GI4502327, Length=248, Percent_Identity=35.8870967741936, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI68989263, Length=217, Percent_Identity=33.6405529953917, Blast_Score=114, Evalue=8e-26,
Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=27.1653543307087, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=31.5573770491803, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI20127408, Length=180, Percent_Identity=35, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI37594469, Length=188, Percent_Identity=31.9148936170213, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI37594471, Length=188, Percent_Identity=31.9148936170213, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=29.6992481203008, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI261878539, Length=126, Percent_Identity=36.5079365079365, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157694516, Length=241, Percent_Identity=24.0663900414938, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI213417737, Length=241, Percent_Identity=24.0663900414938, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI25453481, Length=262, Percent_Identity=24.4274809160305, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI51538573, Length=262, Percent_Identity=24.4274809160305, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI221136756, Length=263, Percent_Identity=23.574144486692, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221136753, Length=263, Percent_Identity=23.574144486692, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI4757968, Length=262, Percent_Identity=24.0458015267176, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI49355787, Length=262, Percent_Identity=24.0458015267176, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221307494, Length=266, Percent_Identity=23.3082706766917, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4757966, Length=196, Percent_Identity=27.0408163265306, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI51538581, Length=196, Percent_Identity=27.0408163265306, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI62530384, Length=212, Percent_Identity=26.8867924528302, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI116235487, Length=208, Percent_Identity=26.4423076923077, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=42.96875, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI221142681, Length=253, Percent_Identity=37.1541501976285, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1788597, Length=266, Percent_Identity=34.5864661654135, Blast_Score=120, Evalue=8e-29,
Organism=Escherichia coli, GI1790281, Length=191, Percent_Identity=34.0314136125654, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=31.5175097276265, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788682, Length=252, Percent_Identity=30.952380952381, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=30.9160305343511, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=51.5748031496063, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=47.3251028806584, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17540714, Length=228, Percent_Identity=31.140350877193, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=257, Percent_Identity=29.1828793774319, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17549921, Length=205, Percent_Identity=31.219512195122, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=263, Percent_Identity=28.1368821292776, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=183, Percent_Identity=30.0546448087432, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=27.9527559055118, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144157, Length=215, Percent_Identity=31.6279069767442, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=215, Percent_Identity=31.6279069767442, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=231, Percent_Identity=27.7056277056277, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=235, Percent_Identity=28.5106382978723, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17508951, Length=235, Percent_Identity=28.5106382978723, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=209, Percent_Identity=26.7942583732057, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=260, Percent_Identity=23.4615384615385, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=202, Percent_Identity=29.2079207920792, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20129971, Length=261, Percent_Identity=55.1724137931034, Blast_Score=289, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24653477, Length=261, Percent_Identity=55.1724137931034, Blast_Score=289, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=34.2412451361868, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=32.8638497652582, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=26.7441860465116, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21357171, Length=234, Percent_Identity=30.3418803418803, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24654903, Length=226, Percent_Identity=26.9911504424779, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19920382, Length=226, Percent_Identity=30.5309734513274, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI28571729, Length=230, Percent_Identity=28.2608695652174, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571730, Length=230, Percent_Identity=28.2608695652174, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921000, Length=182, Percent_Identity=33.5164835164835, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583077, Length=182, Percent_Identity=33.5164835164835, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24583079, Length=182, Percent_Identity=33.5164835164835, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24583165, Length=165, Percent_Identity=27.8787878787879, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19921018, Length=198, Percent_Identity=26.7676767676768, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27610; Mature: 27479

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHNFETILTERIDRVAVITLNRPKALNALNSQVMNEVTTAAAEFDADHGIGAIIITGS
CCCCHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
EKAFAAGADIKEMSEQSFSDMFGSDFFSAWGKLGAVRTPTIAAVSGYALGGGCELAMMCD
CCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEECCCCHHHHHHH
VIIAAENATFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDMILTGRNMDAAEAERSGLVS
HHHEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
RVVATESLLDEAKAVAKTISEMSLSASMMAKEAVNRAFESSLAEGLLFERRIFHSAFGTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DQSEGMAAFVEKRPANFIHR
CCCCCHHHHHHHCCHHHCCC
>Mature Secondary Structure 
TSHNFETILTERIDRVAVITLNRPKALNALNSQVMNEVTTAAAEFDADHGIGAIIITGS
CCCHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
EKAFAAGADIKEMSEQSFSDMFGSDFFSAWGKLGAVRTPTIAAVSGYALGGGCELAMMCD
CCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEECCCCHHHHHHH
VIIAAENATFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDMILTGRNMDAAEAERSGLVS
HHHEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
RVVATESLLDEAKAVAKTISEMSLSASMMAKEAVNRAFESSLAEGLLFERRIFHSAFGTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DQSEGMAAFVEKRPANFIHR
CCCCCHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]