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The map label for this gene is eno

Identifier: 169628258

GI number: 169628258

Start: 1181599

End: 1182891

Strand: Direct

Name: eno

Synonym: MAB_1165

Alternate gene names: 169628258

Gene position: 1181599-1182891 (Clockwise)

Preceding gene: 169628257

Following gene: 169628259

Centisome position: 23.32

GC content: 66.9

Gene sequence:

>1293_bases
GTGCCGATTCTTGAGCAGGTAGGCGCCCGCGAGATCCTCGACTCACGCGGCAACCCGACCGTCGAGGTCGAGGTCGCCTT
GACCGATGGCACGTTCGCACGGGCGGCCGTCCCCTCCGGTGCTTCCACCGGCGAGCATGAGGCCGTTGAGCTGCGCGACG
GCGATGCCCGGTACGGCGGCAAGGGTGTCACCAAGGCCGTCAACGCCGTGCTGGACGAGATCGCCCCGGCCATCATCGGC
GAGAGCGCCGACGATCAGCGCCTCATCGATCAGGCCCTACTGGACCTGGATGGCACTCCGGACAAGTCACGGCTGGGTGC
CAACGCCATCTTGGGTGTCTCGCTGGCCGTGGCCAAGGCCGCTGCCGATTCGGCCGGGCTGGCCCTGTTCCGCTACCTGG
GCGGGCCGAACGCACACATCCTGCCGGTGCCGATGATGAACATCCTCAACGGTGGCGCGCATGCTGATACCGGGGTGGAT
GTGCAGGAGTTCATGGTGGCGCCGATCGGTGCCCCGAGCTTCAAGGAGTCCCTGCGGTGGGGCACCGAGGTCTACCACTC
GCTGAAGTCGGTGCTCAAGAAGCAGGGCCTGTCCACCGGCCTGGGCGACGAGGGCGGGTTTGCCCCCGACGTCGCCGGGA
CGCGGGCCGCGCTGGACCTGATCAGCACCGCCATCGAGGCGACCGGCCTCAAGCTGGGCGCGGATGTGGCGCTGGCGCTG
GATGTCGCCGCCACCGAGTTCTACAGCGCCGCAGACGGTTACAGCTTTGAGAAGGAGAAGCGCACCGCCGAGCAGATGGG
TGCGTTCTACGCCGAGCTGCTCGACGCTTACCCGCTGGTGTCCATCGAGGACCCGCTGTCGGAGGACGACTGGGACGGCT
GGGTCGCGCTCACCACGGCGATCGGCGACCGCGTGCAGTTGGTGGGCGACGATCTGTTTGTCACCAATCCGGAGCGCCTG
GAAGAAGGCATTGAGCGGGGTGCGGCGAATGCCTTGCTGGTCAAGGTGAACCAGATCGGCACGCTCACCGAGACGTTGGA
CGCGGTGACGCTGGCGCACAGCAGTGGCTACAAGACCATGATGAGCCACCGCAGTGGCGAGACCGAAGACACCACCATCG
CCGACCTGGCGGTCGCGGTGGGCAGCGGTCAGATCAAGACCGGCGCTCCGGCGCGTAGCGAGCGAGTGGCCAAGTACAAC
CAGTTGCTGCGTATCGAGGAAACGCTCGGGGACGCTGCCCGTTTCGCGGGAGACCTTGCCTTTCCCCGTTTTTCGATCGA
GCCCGCCAACTAG

Upstream 100 bases:

>100_bases
CCGAACAGGTACGGGACTGGGCAACGGCCTACGCCAAGGGTGCGACGCTGTAGCACTAGTCTGTTACCCGACCACCGGAG
CACCCCCCGAAGGAGAGACA

Downstream 100 bases:

>100_bases
CCGGTTCTGCTGAGGCCGATCCATGGCGGATTCGAAGCGCCGCCCCGCGTCCGGTAGGTCGCGGGGGCCGGCCCCGGCTG
CGCGTAAGCGGACGCCGGTC

Product: enolase (Eno)

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPILEQVGAREILDSRGNPTVEVEVALTDGTFARAAVPSGASTGEHEAVELRDGDARYGGKGVTKAVNAVLDEIAPAIIG
ESADDQRLIDQALLDLDGTPDKSRLGANAILGVSLAVAKAAADSAGLALFRYLGGPNAHILPVPMMNILNGGAHADTGVD
VQEFMVAPIGAPSFKESLRWGTEVYHSLKSVLKKQGLSTGLGDEGGFAPDVAGTRAALDLISTAIEATGLKLGADVALAL
DVAATEFYSAADGYSFEKEKRTAEQMGAFYAELLDAYPLVSIEDPLSEDDWDGWVALTTAIGDRVQLVGDDLFVTNPERL
EEGIERGAANALLVKVNQIGTLTETLDAVTLAHSSGYKTMMSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYN
QLLRIEETLGDAARFAGDLAFPRFSIEPAN

Sequences:

>Translated_430_residues
MPILEQVGAREILDSRGNPTVEVEVALTDGTFARAAVPSGASTGEHEAVELRDGDARYGGKGVTKAVNAVLDEIAPAIIG
ESADDQRLIDQALLDLDGTPDKSRLGANAILGVSLAVAKAAADSAGLALFRYLGGPNAHILPVPMMNILNGGAHADTGVD
VQEFMVAPIGAPSFKESLRWGTEVYHSLKSVLKKQGLSTGLGDEGGFAPDVAGTRAALDLISTAIEATGLKLGADVALAL
DVAATEFYSAADGYSFEKEKRTAEQMGAFYAELLDAYPLVSIEDPLSEDDWDGWVALTTAIGDRVQLVGDDLFVTNPERL
EEGIERGAANALLVKVNQIGTLTETLDAVTLAHSSGYKTMMSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYN
QLLRIEETLGDAARFAGDLAFPRFSIEPAN
>Mature_429_residues
PILEQVGAREILDSRGNPTVEVEVALTDGTFARAAVPSGASTGEHEAVELRDGDARYGGKGVTKAVNAVLDEIAPAIIGE
SADDQRLIDQALLDLDGTPDKSRLGANAILGVSLAVAKAAADSAGLALFRYLGGPNAHILPVPMMNILNGGAHADTGVDV
QEFMVAPIGAPSFKESLRWGTEVYHSLKSVLKKQGLSTGLGDEGGFAPDVAGTRAALDLISTAIEATGLKLGADVALALD
VAATEFYSAADGYSFEKEKRTAEQMGAFYAELLDAYPLVSIEDPLSEDDWDGWVALTTAIGDRVQLVGDDLFVTNPERLE
EGIERGAANALLVKVNQIGTLTETLDAVTLAHSSGYKTMMSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYNQ
LLRIEETLGDAARFAGDLAFPRFSIEPAN

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=54.1860465116279, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=53.3799533799534, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=53.3799533799534, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=51.2761020881671, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=49.6487119437939, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=24.7761194029851, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=24.7761194029851, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=24.7761194029851, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI310129182, Length=125, Percent_Identity=32.8, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI310110045, Length=125, Percent_Identity=32.8, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI310120572, Length=125, Percent_Identity=32.8, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1789141, Length=426, Percent_Identity=56.1032863849765, Blast_Score=456, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI71995829, Length=434, Percent_Identity=52.073732718894, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=434, Percent_Identity=52.073732718894, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=50.261780104712, Blast_Score=191, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=51.8433179723502, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.8845265588915, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.8845265588915, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.8845265588915, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=51.1574074074074, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=51.9721577726218, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=51.9721577726218, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=51.9721577726218, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=51.9721577726218, Blast_Score=400, Evalue=1e-111,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=51.9721577726218, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=51.9721577726218, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_MYCA9 (B1MKG1)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001701907.1
- ProteinModelPortal:   B1MKG1
- SMR:   B1MKG1
- EnsemblBacteria:   EBMYCT00000008888
- GeneID:   5963689
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_1165
- GeneTree:   EBGT00050000016239
- HOGENOM:   HBG726599
- OMA:   GSHADSN
- BioCyc:   MABS561007:MAB_1165-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45066; Mature: 44935

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 335-335 BINDING 154-154 BINDING 163-163 BINDING 283-283 BINDING 310-310 BINDING 335-335 BINDING 386-386

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPILEQVGAREILDSRGNPTVEVEVALTDGTFARAAVPSGASTGEHEAVELRDGDARYGG
CCCHHHCCHHHHHHCCCCCEEEEEEEEECCCCHHHCCCCCCCCCCCCEEEEECCCCCCCC
KGVTKAVNAVLDEIAPAIIGESADDQRLIDQALLDLDGTPDKSRLGANAILGVSLAVAKA
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHH
AADSAGLALFRYLGGPNAHILPVPMMNILNGGAHADTGVDVQEFMVAPIGAPSFKESLRW
HCCHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHH
GTEVYHSLKSVLKKQGLSTGLGDEGGFAPDVAGTRAALDLISTAIEATGLKLGADVALAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCEEEEH
DVAATEFYSAADGYSFEKEKRTAEQMGAFYAELLDAYPLVSIEDPLSEDDWDGWVALTTA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEH
IGDRVQLVGDDLFVTNPERLEEGIERGAANALLVKVNQIGTLTETLDAVTLAHSSGYKTM
HCCEEEEECCCEEECCHHHHHHHHHCCCCCEEEEEECHHCCHHHHHHHHHHHCCCCHHHH
MSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYNQLLRIEETLGDAARFAGDLA
HHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FPRFSIEPAN
CCCEECCCCC
>Mature Secondary Structure 
PILEQVGAREILDSRGNPTVEVEVALTDGTFARAAVPSGASTGEHEAVELRDGDARYGG
CCHHHCCHHHHHHCCCCCEEEEEEEEECCCCHHHCCCCCCCCCCCCEEEEECCCCCCCC
KGVTKAVNAVLDEIAPAIIGESADDQRLIDQALLDLDGTPDKSRLGANAILGVSLAVAKA
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHH
AADSAGLALFRYLGGPNAHILPVPMMNILNGGAHADTGVDVQEFMVAPIGAPSFKESLRW
HCCHHHHHHHHHHCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHH
GTEVYHSLKSVLKKQGLSTGLGDEGGFAPDVAGTRAALDLISTAIEATGLKLGADVALAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEECCCEEEEH
DVAATEFYSAADGYSFEKEKRTAEQMGAFYAELLDAYPLVSIEDPLSEDDWDGWVALTTA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEH
IGDRVQLVGDDLFVTNPERLEEGIERGAANALLVKVNQIGTLTETLDAVTLAHSSGYKTM
HCCEEEEECCCEEECCHHHHHHHHHCCCCCEEEEEECHHCCHHHHHHHHHHHCCCCHHHH
MSHRSGETEDTTIADLAVAVGSGQIKTGAPARSERVAKYNQLLRIEETLGDAARFAGDLA
HHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FPRFSIEPAN
CCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA