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The map label for this gene is pcrA [H]

Identifier: 169628149

GI number: 169628149

Start: 1061713

End: 1064136

Strand: Direct

Name: pcrA [H]

Synonym: MAB_1054

Alternate gene names: 169628149

Gene position: 1061713-1064136 (Clockwise)

Preceding gene: 169628144

Following gene: 169628151

Centisome position: 20.95

GC content: 63.99

Gene sequence:

>2424_bases
ATGGCGAATCTTTCCGAACCAGCACTTTTTGACATGCCCAGCGACTCGAGTGCCTCCAGCGACGCGTTGCTCGACGGCCT
GAACCCGCAGCAGCGGGCTGCCGTCGCCCACCAGGGCGCACCGCTGCTCATTGTCGCCGGTGCCGGCTCGGGCAAGACCG
CGGTGCTGACCCGTCGCATCGCGTACCTGCTGGCCGCGCGGGACGTCAGCCCCGGTCAGATCCTGGCGATCACCTTCACC
AACAAGGCGGCGGCCGAGATGCGCGAGCGCGTTGGGCGGATCGTGGCGAATCGGGTGCAGTCCATGTGGGTGTCGACGTT
CCACTCCAGCTGCGTGCGCATCCTGCGTAACCAGGCCTCGCTGCTGCCCGGGCTCAACTCCAATTTCTCGATATACGACT
CGGACGATTCACGGCGGCTGCTGCAGATGATCGGTCGCGATATGGGCCTGGACATCAAGCGCTATTCGCCACGGCTGCTC
GCCACCGCGATCTCCAATCTGAAGAACGAGCTCATCTCTCCCGAACAGGCGGTGGCCAACCTCACCGCCGGCGGCCAGGA
TGGGGAGCTGCGCGGGGATCTTCCGCAGATCGTGGCCGACGTGTACGGCGAGTACCAGCGCCGGCTGCGCGCCGCCAACG
CCTTGGACTTCGACGATCTGATCGGCGAGACCGTGGCCGTGCTGCAGCAGTTCCCGCAGATCGCGCAGTTCTATCGCCGC
AAGTTCCGGCACATCCTGGTTGACGAGTACCAGGACACCAACCACGCCCAGTACGTGTTGGTCCGCGAGCTGGTGGGAGC
TCATGTCGACCGCACTGTGGACCCGGATGGTCCGGAGCCGGCCGAGCTGTGCGTGGTGGGTGACGCCGACCAGTCCATCT
ACGCGTTCCGCGGCGCGACCATCCGCAATATCGAAGAATTCGAGCGCGACTATCCGAACGCGAAAACCATTCTGCTGGAG
CAAAACTATCGGTCCACGCAGAACATATTGAGTGCGGCGAACTCGGTCATCGCCAAAAATGCGAACCGCCGGGAGAAGCG
GTTATGGACCGATTCCGGCGAGGGAGAGCTGATCGTCGGCTATGTCGCGGACAACGAACACGACGAGGCGTCCTTCATCG
CCCGCGAGATCGACGGGCTCTTCGATCGCAGCGATGCCAACTATGGTGACATCGCGGTGTTTTACCGCACCAACAACAAT
TCCCGGTCGCTGGAAGAGATCTTCATCCGTACCGGTATTCCGTACAAAGTTGTTGGGGGAGTGAGGTTTTACGAACGCAA
GGAAATTCGCGACATCGTCGCCTACCTGCGGGTGCTGACCAACCCGGGCGATGCGGTGAGCCTGCGGCGGATCCTCAACA
CCCCTCGCCGGGGTATCGGCGACCGGGCCGAGGCCTGTGTCGCGGTACACGCCGAGACCAATGGCATCGGATTCGGGGAA
GCGCTGGCCGACGCCGCCGCGGGCAAGGTGGCGATGCTGAACTCTCGTTCCGAGAAGGCAATTGCGGCCTTCATGGATAT
GCTCGACCAGATCCGTGTCACCCTGCGTACCGATGATCCGGGAGCGCTGCCCGATATCGGCGACGTGGTAGAGGCCGTAC
TGAACCACACCGGATATCGCTACGAACTGGAGAACAGCAGCGATCCGCAGGAGCTGGCACGTCTTGACAACCTCAATGAA
TTGGTCAGCGTCGCACATGAATTCAGTGCGGACGCGGCCAATGCGCAGGCCGCCGGGGAAGACGTGCCGGGTGACGATGA
GGATGTCGGTGCCCCTCCGGGCAGCCTCGAGGCATTCCTGGAGCGGGTATCCCTGGTCGCCGATGCGGACGAGCTGCCCG
AGTCCAGCGCCGGCGTCGTCACCATGATGACCCTGCACACCGCCAAGGGGCTGGAGTTCCCGGTGGTGTTCGTGACCGGC
TGGGAAGACGGCATGTTCCCGCATATGCGGGCACTGGGTGACCCGGTGGAGCTCGCCGAGGAACGGCGGCTGGCCTATGT
CGGGATCACCCGGGCTCGCCAGCGTCTCTATCTGAGCCGGGCCAAAGTTCGTTCGTCGTGGGGGCAGCCCATGATGAATC
CGGAATCACGCTTCCTGCAAGAGATTCCGCAAGAACTTATCGACTGGCGGCGCACCGATCCGCTGCCTGGGCGGATCGCG
ACGGGCGCCGGCGGTTACGGCGGTGGAGGGTACGGATCCGGCGGATCGCGTGCGGGCGGCGGCTCGCTGGGCGCTCCCTC
CAAAAAACGCACCAAGCCGCTGATGGTGCTGGAGGCCGGGGACCGGGTGAGCCACGACAAGTACGGATTGGGTCGCGTCG
TGGAGGTGACCGGTGTCGGCGAGAGCGCCACTTCGCTCATCGACTTCGGCAGTGAGGGCCGCGTGAAGTTGATGCACAAC
TTCGCACCCGTGCAGAAGCTCTAG

Upstream 100 bases:

>100_bases
TGGTCAAGCACATCGAAGAGTGTAGCCGACATGCGGTGAATGCCGGCGTGAAGAGCATCAAGCCGAGATTGTCCCTTGTC
AGCGGTAAGTTAGATCGGTC

Downstream 100 bases:

>100_bases
AACTCACAACGGGCCAGCCGAGCCATGCGGGCCCGCTGTGATGGCACGGCAGGGCAAAAAAGGCCGGGTGTGAGCAACGT
TGCTCACACCCGGCCTTGGA

Product: putative ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MANLSEPALFDMPSDSSASSDALLDGLNPQQRAAVAHQGAPLLIVAGAGSGKTAVLTRRIAYLLAARDVSPGQILAITFT
NKAAAEMRERVGRIVANRVQSMWVSTFHSSCVRILRNQASLLPGLNSNFSIYDSDDSRRLLQMIGRDMGLDIKRYSPRLL
ATAISNLKNELISPEQAVANLTAGGQDGELRGDLPQIVADVYGEYQRRLRAANALDFDDLIGETVAVLQQFPQIAQFYRR
KFRHILVDEYQDTNHAQYVLVRELVGAHVDRTVDPDGPEPAELCVVGDADQSIYAFRGATIRNIEEFERDYPNAKTILLE
QNYRSTQNILSAANSVIAKNANRREKRLWTDSGEGELIVGYVADNEHDEASFIAREIDGLFDRSDANYGDIAVFYRTNNN
SRSLEEIFIRTGIPYKVVGGVRFYERKEIRDIVAYLRVLTNPGDAVSLRRILNTPRRGIGDRAEACVAVHAETNGIGFGE
ALADAAAGKVAMLNSRSEKAIAAFMDMLDQIRVTLRTDDPGALPDIGDVVEAVLNHTGYRYELENSSDPQELARLDNLNE
LVSVAHEFSADAANAQAAGEDVPGDDEDVGAPPGSLEAFLERVSLVADADELPESSAGVVTMMTLHTAKGLEFPVVFVTG
WEDGMFPHMRALGDPVELAEERRLAYVGITRARQRLYLSRAKVRSSWGQPMMNPESRFLQEIPQELIDWRRTDPLPGRIA
TGAGGYGGGGYGSGGSRAGGGSLGAPSKKRTKPLMVLEAGDRVSHDKYGLGRVVEVTGVGESATSLIDFGSEGRVKLMHN
FAPVQKL

Sequences:

>Translated_807_residues
MANLSEPALFDMPSDSSASSDALLDGLNPQQRAAVAHQGAPLLIVAGAGSGKTAVLTRRIAYLLAARDVSPGQILAITFT
NKAAAEMRERVGRIVANRVQSMWVSTFHSSCVRILRNQASLLPGLNSNFSIYDSDDSRRLLQMIGRDMGLDIKRYSPRLL
ATAISNLKNELISPEQAVANLTAGGQDGELRGDLPQIVADVYGEYQRRLRAANALDFDDLIGETVAVLQQFPQIAQFYRR
KFRHILVDEYQDTNHAQYVLVRELVGAHVDRTVDPDGPEPAELCVVGDADQSIYAFRGATIRNIEEFERDYPNAKTILLE
QNYRSTQNILSAANSVIAKNANRREKRLWTDSGEGELIVGYVADNEHDEASFIAREIDGLFDRSDANYGDIAVFYRTNNN
SRSLEEIFIRTGIPYKVVGGVRFYERKEIRDIVAYLRVLTNPGDAVSLRRILNTPRRGIGDRAEACVAVHAETNGIGFGE
ALADAAAGKVAMLNSRSEKAIAAFMDMLDQIRVTLRTDDPGALPDIGDVVEAVLNHTGYRYELENSSDPQELARLDNLNE
LVSVAHEFSADAANAQAAGEDVPGDDEDVGAPPGSLEAFLERVSLVADADELPESSAGVVTMMTLHTAKGLEFPVVFVTG
WEDGMFPHMRALGDPVELAEERRLAYVGITRARQRLYLSRAKVRSSWGQPMMNPESRFLQEIPQELIDWRRTDPLPGRIA
TGAGGYGGGGYGSGGSRAGGGSLGAPSKKRTKPLMVLEAGDRVSHDKYGLGRVVEVTGVGESATSLIDFGSEGRVKLMHN
FAPVQKL
>Mature_806_residues
ANLSEPALFDMPSDSSASSDALLDGLNPQQRAAVAHQGAPLLIVAGAGSGKTAVLTRRIAYLLAARDVSPGQILAITFTN
KAAAEMRERVGRIVANRVQSMWVSTFHSSCVRILRNQASLLPGLNSNFSIYDSDDSRRLLQMIGRDMGLDIKRYSPRLLA
TAISNLKNELISPEQAVANLTAGGQDGELRGDLPQIVADVYGEYQRRLRAANALDFDDLIGETVAVLQQFPQIAQFYRRK
FRHILVDEYQDTNHAQYVLVRELVGAHVDRTVDPDGPEPAELCVVGDADQSIYAFRGATIRNIEEFERDYPNAKTILLEQ
NYRSTQNILSAANSVIAKNANRREKRLWTDSGEGELIVGYVADNEHDEASFIAREIDGLFDRSDANYGDIAVFYRTNNNS
RSLEEIFIRTGIPYKVVGGVRFYERKEIRDIVAYLRVLTNPGDAVSLRRILNTPRRGIGDRAEACVAVHAETNGIGFGEA
LADAAAGKVAMLNSRSEKAIAAFMDMLDQIRVTLRTDDPGALPDIGDVVEAVLNHTGYRYELENSSDPQELARLDNLNEL
VSVAHEFSADAANAQAAGEDVPGDDEDVGAPPGSLEAFLERVSLVADADELPESSAGVVTMMTLHTAKGLEFPVVFVTGW
EDGMFPHMRALGDPVELAEERRLAYVGITRARQRLYLSRAKVRSSWGQPMMNPESRFLQEIPQELIDWRRTDPLPGRIAT
GAGGYGGGGYGSGGSRAGGGSLGAPSKKRTKPLMVLEAGDRVSHDKYGLGRVVEVTGVGESATSLIDFGSEGRVKLMHNF
APVQKL

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=785, Percent_Identity=36.8152866242038, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI48994965, Length=689, Percent_Identity=37.8809869375907, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1787196, Length=348, Percent_Identity=27.5862068965517, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=777, Percent_Identity=29.0862290862291, Blast_Score=238, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6324477, Length=486, Percent_Identity=25.3086419753086, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 88063; Mature: 87932

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLSEPALFDMPSDSSASSDALLDGLNPQQRAAVAHQGAPLLIVAGAGSGKTAVLTRRI
CCCCCCCCEEECCCCCCCCCHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
AYLLAARDVSPGQILAITFTNKAAAEMRERVGRIVANRVQSMWVSTFHSSCVRILRNQAS
HHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLPGLNSNFSIYDSDDSRRLLQMIGRDMGLDIKRYSPRLLATAISNLKNELISPEQAVAN
HCCCCCCCCEEEECCHHHHHHHHHCHHCCCCHHHCCHHHHHHHHHHHHHHHCCHHHHHHH
LTAGGQDGELRGDLPQIVADVYGEYQRRLRAANALDFDDLIGETVAVLQQFPQIAQFYRR
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KFRHILVDEYQDTNHAQYVLVRELVGAHVDRTVDPDGPEPAELCVVGDADQSIYAFRGAT
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEECCCH
IRNIEEFERDYPNAKTILLEQNYRSTQNILSAANSVIAKNANRREKRLWTDSGEGELIVG
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEE
YVADNEHDEASFIAREIDGLFDRSDANYGDIAVFYRTNNNSRSLEEIFIRTGIPYKVVGG
EECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEECC
VRFYERKEIRDIVAYLRVLTNPGDAVSLRRILNTPRRGIGDRAEACVAVHAETNGIGFGE
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHH
ALADAAAGKVAMLNSRSEKAIAAFMDMLDQIRVTLRTDDPGALPDIGDVVEAVLNHTGYR
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHCCCCE
YELENSSDPQELARLDNLNELVSVAHEFSADAANAQAAGEDVPGDDEDVGAPPGSLEAFL
EEECCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ERVSLVADADELPESSAGVVTMMTLHTAKGLEFPVVFVTGWEDGMFPHMRALGDPVELAE
HHHHHHHCHHHCCCCCCCEEEEEEEHHCCCCCCCEEEEECCCCCCCHHHHHCCCHHHHHH
ERRLAYVGITRARQRLYLSRAKVRSSWGQPMMNPESRFLQEIPQELIDWRRTDPLPGRIA
HCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEE
TGAGGYGGGGYGSGGSRAGGGSLGAPSKKRTKPLMVLEAGDRVSHDKYGLGRVVEVTGVG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCC
ESATSLIDFGSEGRVKLMHNFAPVQKL
CHHHHHHHCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
ANLSEPALFDMPSDSSASSDALLDGLNPQQRAAVAHQGAPLLIVAGAGSGKTAVLTRRI
CCCCCCCEEECCCCCCCCCHHHHCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
AYLLAARDVSPGQILAITFTNKAAAEMRERVGRIVANRVQSMWVSTFHSSCVRILRNQAS
HHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLPGLNSNFSIYDSDDSRRLLQMIGRDMGLDIKRYSPRLLATAISNLKNELISPEQAVAN
HCCCCCCCCEEEECCHHHHHHHHHCHHCCCCHHHCCHHHHHHHHHHHHHHHCCHHHHHHH
LTAGGQDGELRGDLPQIVADVYGEYQRRLRAANALDFDDLIGETVAVLQQFPQIAQFYRR
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KFRHILVDEYQDTNHAQYVLVRELVGAHVDRTVDPDGPEPAELCVVGDADQSIYAFRGAT
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEECCCH
IRNIEEFERDYPNAKTILLEQNYRSTQNILSAANSVIAKNANRREKRLWTDSGEGELIVG
HHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEE
YVADNEHDEASFIAREIDGLFDRSDANYGDIAVFYRTNNNSRSLEEIFIRTGIPYKVVGG
EECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEECC
VRFYERKEIRDIVAYLRVLTNPGDAVSLRRILNTPRRGIGDRAEACVAVHAETNGIGFGE
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHH
ALADAAAGKVAMLNSRSEKAIAAFMDMLDQIRVTLRTDDPGALPDIGDVVEAVLNHTGYR
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHCCCCE
YELENSSDPQELARLDNLNELVSVAHEFSADAANAQAAGEDVPGDDEDVGAPPGSLEAFL
EEECCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ERVSLVADADELPESSAGVVTMMTLHTAKGLEFPVVFVTGWEDGMFPHMRALGDPVELAE
HHHHHHHCHHHCCCCCCCEEEEEEEHHCCCCCCCEEEEECCCCCCCHHHHHCCCHHHHHH
ERRLAYVGITRARQRLYLSRAKVRSSWGQPMMNPESRFLQEIPQELIDWRRTDPLPGRIA
HCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEE
TGAGGYGGGGYGSGGSRAGGGSLGAPSKKRTKPLMVLEAGDRVSHDKYGLGRVVEVTGVG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCC
ESATSLIDFGSEGRVKLMHNFAPVQKL
CHHHHHHHCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]