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The map label for this gene is fadD21 [H]

Identifier: 169628032

GI number: 169628032

Start: 927298

End: 929016

Strand: Reverse

Name: fadD21 [H]

Synonym: MAB_0935c

Alternate gene names: 169628032

Gene position: 929016-927298 (Counterclockwise)

Preceding gene: 169628033

Following gene: 169628029

Centisome position: 18.33

GC content: 66.61

Gene sequence:

>1719_bases
ATGCTGCACGGACGTGCGAGCCTGCGCCCGGACGACATCGCATTCACCTTCACCGACTACACCAACAGTCCGACCGGTGT
CGCCGAAAGCCTCACCTGGTCTCAGCTGTCTCGCCGCACCTTCAATGTGGCCCGCGAACTCGGCCAGCACGCCTCGCCCG
GCGACCGGGCGCTGATCCTGGCGCCGCAAAGCCTCGAATACATCCTGGCGTTCCTGGGCTCCATGCAGGCCGGACTGATC
GCGGTGCCGCTTCCCTTGCCGCATCGCGGCTCGAGTCTTGACCGGGTCAGTGCCGTCTTCGACGACACCTCGCCCTCGGT
GGTGCTCACAACGTCCGCGGTCGCGTCAGATGTCGGCGACTACGTCGACCAGTCGCGCCTGGAGATCGCGCCCAAGCTCA
TCGAGATCGACACCTTGCGCCTCGACGTGGACGCCGGGCCAAGCCTCGTACCGGGCGACGTCCCCAACATCGCGTACCTG
CAATACAGCTCGGGTTCCACCCGGACACCTACCGGCGTCATGCTCTCGCACCGCAATCTGCAGGTGAACTTCGAGCAGTT
GATGCGCAGCTTCTTCGTGGACACCGGCTGCACCATCCCGTCGAACGCGACGATAGTGTCGTGGTTGCCCTTCTATCACG
ACATGGGCTTGGTGCTGGGCGTCTGCGCTCCGATCCTGGGCGGCTATCGGGCCGACCTGACCAGTCCGCTGGCCTTTCTG
GAAAGCCCGTCGCGCTGGGTGCGCGCGCTGGCCACAAACCCGCACGCCTGGTCCTCCGCTCCCAACTTCGCCTTCGATCT
GGCGGCCCGCAAGACGACCGACGCCGACCTGGCGGGGCTCGACCTGGGCGGCGTGCTGGGCATCATCAGCGGTGCCGAGC
GGGTCGAGCAGGCCACTCTGCAACGCTTCGTCGACCGGTTCGCCCACTTCAATTTCCGGGACTCCATGATGCGCCCCTCC
TACGGGCTGGCGGAGGCGACGGTGTTCGTCGCCTCCGGGACCTGGAGCGAGACAGCACCCGCGGCCCACTTCGACGTCGA
GGAGCTGTCGGCGGGCCGGGTGCGGCGGTGCGCGGCCGGGGCCGGAACGGCGCTGGTCCAGTACAAGGTGCCGCACTCCC
CCGTACTGCGGATCATCGACAGCGACACTCACCGGGAGTGCCCGGCGGACGTGGTCGGCGAGATCTGGGTGCACGGCGAC
AATGTCGCTTCCGGCTATTGGCGTAAGTCCCCCGAGGAGCAGCGGTGCTTCGGCGCGCAGATCGCCGACCCATCCGCCGG
CACCCCGGAGGGGCCCTGGCTCAGGACCGGCGATCTCGGCTTCGTGTCCGAGGGCGAGCTGTTCATCGTCGGCCGCATCA
AGGATCTGCTCATCATCCGCGGGCGCAATTACTACCCCGAGGACATCGAGGCCACCGTTCAGGAGATCAGCCGCGGCCGG
GTCGCGGCCATCTCGGTTCCGTTCGACAGCACCGAGAAGCTGGTCACCATCATCGAGCTCAAGAAGCGCAGCGATACCGA
CATCGAGGCCATCAGGAGCGAAATCACCTCGGCGATCTCGAATGCGCACGGGGTAAACGTCGGAGATGTCGTGCTGGTGC
CACCGGGATCGCTACCCACGACCACCAGCGGCAAGATTCGGCGCCGCTCGTGTGTCGAGCAGTATCTCCAGCATGAGTTC
ATCCGATTGGACGCGGACTCCCCGGTGGGCGTCGCCTAG

Upstream 100 bases:

>100_bases
GCTCTGGCCGTGCTGGGCAACGGCACCCGCCCGACCCCTCCCAATTAAGCATGACTTGGACAAAACTATGACACCTTCAA
CCCAGTCATCCATCCTGTCG

Downstream 100 bases:

>100_bases
CCCCTCTCGGTGTCAGATGCCGGCAAGCCCCACGGCCAGCAATACGCACCCGACCGCCGTCAGCACCAACGCGATATTGC
GGCGGCGGTTGGATTGGATC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MLHGRASLRPDDIAFTFTDYTNSPTGVAESLTWSQLSRRTFNVARELGQHASPGDRALILAPQSLEYILAFLGSMQAGLI
AVPLPLPHRGSSLDRVSAVFDDTSPSVVLTTSAVASDVGDYVDQSRLEIAPKLIEIDTLRLDVDAGPSLVPGDVPNIAYL
QYSSGSTRTPTGVMLSHRNLQVNFEQLMRSFFVDTGCTIPSNATIVSWLPFYHDMGLVLGVCAPILGGYRADLTSPLAFL
ESPSRWVRALATNPHAWSSAPNFAFDLAARKTTDADLAGLDLGGVLGIISGAERVEQATLQRFVDRFAHFNFRDSMMRPS
YGLAEATVFVASGTWSETAPAAHFDVEELSAGRVRRCAAGAGTALVQYKVPHSPVLRIIDSDTHRECPADVVGEIWVHGD
NVASGYWRKSPEEQRCFGAQIADPSAGTPEGPWLRTGDLGFVSEGELFIVGRIKDLLIIRGRNYYPEDIEATVQEISRGR
VAAISVPFDSTEKLVTIIELKKRSDTDIEAIRSEITSAISNAHGVNVGDVVLVPPGSLPTTTSGKIRRRSCVEQYLQHEF
IRLDADSPVGVA

Sequences:

>Translated_572_residues
MLHGRASLRPDDIAFTFTDYTNSPTGVAESLTWSQLSRRTFNVARELGQHASPGDRALILAPQSLEYILAFLGSMQAGLI
AVPLPLPHRGSSLDRVSAVFDDTSPSVVLTTSAVASDVGDYVDQSRLEIAPKLIEIDTLRLDVDAGPSLVPGDVPNIAYL
QYSSGSTRTPTGVMLSHRNLQVNFEQLMRSFFVDTGCTIPSNATIVSWLPFYHDMGLVLGVCAPILGGYRADLTSPLAFL
ESPSRWVRALATNPHAWSSAPNFAFDLAARKTTDADLAGLDLGGVLGIISGAERVEQATLQRFVDRFAHFNFRDSMMRPS
YGLAEATVFVASGTWSETAPAAHFDVEELSAGRVRRCAAGAGTALVQYKVPHSPVLRIIDSDTHRECPADVVGEIWVHGD
NVASGYWRKSPEEQRCFGAQIADPSAGTPEGPWLRTGDLGFVSEGELFIVGRIKDLLIIRGRNYYPEDIEATVQEISRGR
VAAISVPFDSTEKLVTIIELKKRSDTDIEAIRSEITSAISNAHGVNVGDVVLVPPGSLPTTTSGKIRRRSCVEQYLQHEF
IRLDADSPVGVA
>Mature_572_residues
MLHGRASLRPDDIAFTFTDYTNSPTGVAESLTWSQLSRRTFNVARELGQHASPGDRALILAPQSLEYILAFLGSMQAGLI
AVPLPLPHRGSSLDRVSAVFDDTSPSVVLTTSAVASDVGDYVDQSRLEIAPKLIEIDTLRLDVDAGPSLVPGDVPNIAYL
QYSSGSTRTPTGVMLSHRNLQVNFEQLMRSFFVDTGCTIPSNATIVSWLPFYHDMGLVLGVCAPILGGYRADLTSPLAFL
ESPSRWVRALATNPHAWSSAPNFAFDLAARKTTDADLAGLDLGGVLGIISGAERVEQATLQRFVDRFAHFNFRDSMMRPS
YGLAEATVFVASGTWSETAPAAHFDVEELSAGRVRRCAAGAGTALVQYKVPHSPVLRIIDSDTHRECPADVVGEIWVHGD
NVASGYWRKSPEEQRCFGAQIADPSAGTPEGPWLRTGDLGFVSEGELFIVGRIKDLLIIRGRNYYPEDIEATVQEISRGR
VAAISVPFDSTEKLVTIIELKKRSDTDIEAIRSEITSAISNAHGVNVGDVVLVPPGSLPTTTSGKIRRRSCVEQYLQHEF
IRLDADSPVGVA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=539, Percent_Identity=25.417439703154, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI55749758, Length=529, Percent_Identity=25.1417769376182, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI225735629, Length=534, Percent_Identity=25.2808988764045, Blast_Score=114, Evalue=4e-25,
Organism=Homo sapiens, GI45827692, Length=534, Percent_Identity=25.2808988764045, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI225735625, Length=607, Percent_Identity=23.3937397034596, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI45827694, Length=537, Percent_Identity=23.2774674115456, Blast_Score=77, Evalue=6e-14,
Organism=Escherichia coli, GI1788107, Length=579, Percent_Identity=26.0794473229706, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI193202679, Length=600, Percent_Identity=23.8333333333333, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI18859661, Length=528, Percent_Identity=24.2424242424242, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24654656, Length=465, Percent_Identity=23.6559139784946, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI281365686, Length=156, Percent_Identity=31.4102564102564, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 61978; Mature: 61978

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHGRASLRPDDIAFTFTDYTNSPTGVAESLTWSQLSRRTFNVARELGQHASPGDRALIL
CCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
APQSLEYILAFLGSMQAGLIAVPLPLPHRGSSLDRVSAVFDDTSPSVVLTTSAVASDVGD
CCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHH
YVDQSRLEIAPKLIEIDTLRLDVDAGPSLVPGDVPNIAYLQYSSGSTRTPTGVMLSHRNL
HHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
QVNFEQLMRSFFVDTGCTIPSNATIVSWLPFYHDMGLVLGVCAPILGGYRADLTSPLAFL
EECHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ESPSRWVRALATNPHAWSSAPNFAFDLAARKTTDADLAGLDLGGVLGIISGAERVEQATL
CCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QRFVDRFAHFNFRDSMMRPSYGLAEATVFVASGTWSETAPAAHFDVEELSAGRVRRCAAG
HHHHHHHHCCCCHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHCCCHHHHHHCC
AGTALVQYKVPHSPVLRIIDSDTHRECPADVVGEIWVHGDNVASGYWRKSPEEQRCFGAQ
CCCEEEEEECCCCCEEEEECCCCCCCCCHHHHCEEEEECCCCCCCCCCCCCHHHHHCCCC
IADPSAGTPEGPWLRTGDLGFVSEGELFIVGRIKDLLIIRGRNYYPEDIEATVQEISRGR
CCCCCCCCCCCCEEEECCCCEECCCCEEEEEEECEEEEECCCCCCCHHHHHHHHHHHCCC
VAAISVPFDSTEKLVTIIELKKRSDTDIEAIRSEITSAISNAHGVNVGDVVLVPPGSLPT
EEEEECCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCC
TTSGKIRRRSCVEQYLQHEFIRLDADSPVGVA
CCCCCHHHHHHHHHHHHHHEEEECCCCCCCCC
>Mature Secondary Structure
MLHGRASLRPDDIAFTFTDYTNSPTGVAESLTWSQLSRRTFNVARELGQHASPGDRALIL
CCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
APQSLEYILAFLGSMQAGLIAVPLPLPHRGSSLDRVSAVFDDTSPSVVLTTSAVASDVGD
CCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHH
YVDQSRLEIAPKLIEIDTLRLDVDAGPSLVPGDVPNIAYLQYSSGSTRTPTGVMLSHRNL
HHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
QVNFEQLMRSFFVDTGCTIPSNATIVSWLPFYHDMGLVLGVCAPILGGYRADLTSPLAFL
EECHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
ESPSRWVRALATNPHAWSSAPNFAFDLAARKTTDADLAGLDLGGVLGIISGAERVEQATL
CCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
QRFVDRFAHFNFRDSMMRPSYGLAEATVFVASGTWSETAPAAHFDVEELSAGRVRRCAAG
HHHHHHHHCCCCHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHCCCHHHHHHCC
AGTALVQYKVPHSPVLRIIDSDTHRECPADVVGEIWVHGDNVASGYWRKSPEEQRCFGAQ
CCCEEEEEECCCCCEEEEECCCCCCCCCHHHHCEEEEECCCCCCCCCCCCCHHHHHCCCC
IADPSAGTPEGPWLRTGDLGFVSEGELFIVGRIKDLLIIRGRNYYPEDIEATVQEISRGR
CCCCCCCCCCCCEEEECCCCEECCCCEEEEEEECEEEEECCCCCCCHHHHHHHHHHHCCC
VAAISVPFDSTEKLVTIIELKKRSDTDIEAIRSEITSAISNAHGVNVGDVVLVPPGSLPT
EEEEECCCCCCHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCC
TTSGKIRRRSCVEQYLQHEFIRLDADSPVGVA
CCCCCHHHHHHHHHHHHHHEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]