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The map label for this gene is citA [H]

Identifier: 169628029

GI number: 169628029

Start: 924733

End: 925869

Strand: Reverse

Name: citA [H]

Synonym: MAB_0932c

Alternate gene names: 169628029

Gene position: 925869-924733 (Counterclockwise)

Preceding gene: 169628032

Following gene: 169628028

Centisome position: 18.27

GC content: 67.72

Gene sequence:

>1137_bases
ATGACGGTTGCAGCGCCGCTACCAGCGGATTTCGCACCTGGACTGGAGGGCGTAACCGCCTTCGCCACCGAGATCGCCGA
GCCGGACAAGGACGGTGGTTCACTGCGCTATCGCGGTGTCGACATCGAGGATCTGGTCGCCCAGCGGGTGACCTTCGGTG
ATGTATGGGCACTGTTGGTCGACGGCAAGTTCGGACAGGGGCTCCCACCGGCCGAACCTTTCCCCATCCCCGTGCACACC
GGCGACGTCCGCGTGGACGTACAGGCCGGGCTGGCCATGCTGGCACCTATCTGGGGTTTCGAGCCGCTGCTGGACACCGA
GGATTCGGTGGCGCGCAGTCAGCTCGCGCGCGCCTCGGTCATGGCGCTCTCGTACGTCGCGCAGTCGGCGCGAGGCAATT
CCCTGCCCGCCGTTCCGCAACACATTGTCGACGAATGTTCAACCGTCACATCGCGTTTCATGACCCGCTGGCAGGGTGAT
CCCGATCCCAAGCATGTCGAGGCCATTGACGCGTACTGGGTATCGGCCGCCGAGCACGGCATGAACGCCTCGACGTTCAC
TGCCCGCGTGATCGCCTCCACCGGCGCAGATGTGGCCGCGTCGCTCTCCGGCGCGGTCGGAGCGATGAGCGGGCCATTGC
ATGGCGGCGCCCCCGCCCGCGTACTGCCGATGATCGAGCAGGCCGAGAAGACCGGCGATGCCCGCGGCGTCATCAGGGGC
ATTCTGGACCGCCGCGAAAAGCTCATGGGCTTCGGCCACCGGGTGTATCGCGCCGAAGACCCGCGCGCGCGGGTGCTGCG
CAGCACCGCCAAGCGCCTGGGCGCCGCGCGCTATGAGGTTGCCGCCGCCCTTGAGCAGGCTGCCCTGGCCGAGCTGCGAG
AACGCCGCCCCGATCGCGTCATCGAGACCAATGTCGAGTTCTGGGCGGCGGTCATTCTCGACTTCGCGCAGGTGCCGACG
CGGATGATGCCCGCCATGTTCACCTGCGCCCGCACCGCGGGCTGGAGTGCGCACATCATGGAGCAGAAACGTCTCGGCAA
GTTGGTGCGCCCGGCCGCGCTGTATGTGGGGCCCGGACCGCGCCCCATCGAATCCGTCGAGGGATTCGGGCTGCTGCACT
CTCGCGTCGGTGCGTAA

Upstream 100 bases:

>100_bases
CCCGGATAAGGCACTGCGGGGTGACTAAGAAGCACCCTTATTTGCGAGTGCAAGAATCGCCTTTACCGAGTTGTTACCCA
CAGGTAGGGAGTCGAGAATC

Downstream 100 bases:

>100_bases
CTACCCGAACTCCTGGCGTCACTCTGCGTGAGGTGGTTGGGTGAGTCTTCATGGAACCCTCGCAGACCTCTCCCGACCTC
CCACCAGTGCAACCCTCCGT

Product: citrate synthase 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTVAAPLPADFAPGLEGVTAFATEIAEPDKDGGSLRYRGVDIEDLVAQRVTFGDVWALLVDGKFGQGLPPAEPFPIPVHT
GDVRVDVQAGLAMLAPIWGFEPLLDTEDSVARSQLARASVMALSYVAQSARGNSLPAVPQHIVDECSTVTSRFMTRWQGD
PDPKHVEAIDAYWVSAAEHGMNASTFTARVIASTGADVAASLSGAVGAMSGPLHGGAPARVLPMIEQAEKTGDARGVIRG
ILDRREKLMGFGHRVYRAEDPRARVLRSTAKRLGAARYEVAAALEQAALAELRERRPDRVIETNVEFWAAVILDFAQVPT
RMMPAMFTCARTAGWSAHIMEQKRLGKLVRPAALYVGPGPRPIESVEGFGLLHSRVGA

Sequences:

>Translated_378_residues
MTVAAPLPADFAPGLEGVTAFATEIAEPDKDGGSLRYRGVDIEDLVAQRVTFGDVWALLVDGKFGQGLPPAEPFPIPVHT
GDVRVDVQAGLAMLAPIWGFEPLLDTEDSVARSQLARASVMALSYVAQSARGNSLPAVPQHIVDECSTVTSRFMTRWQGD
PDPKHVEAIDAYWVSAAEHGMNASTFTARVIASTGADVAASLSGAVGAMSGPLHGGAPARVLPMIEQAEKTGDARGVIRG
ILDRREKLMGFGHRVYRAEDPRARVLRSTAKRLGAARYEVAAALEQAALAELRERRPDRVIETNVEFWAAVILDFAQVPT
RMMPAMFTCARTAGWSAHIMEQKRLGKLVRPAALYVGPGPRPIESVEGFGLLHSRVGA
>Mature_377_residues
TVAAPLPADFAPGLEGVTAFATEIAEPDKDGGSLRYRGVDIEDLVAQRVTFGDVWALLVDGKFGQGLPPAEPFPIPVHTG
DVRVDVQAGLAMLAPIWGFEPLLDTEDSVARSQLARASVMALSYVAQSARGNSLPAVPQHIVDECSTVTSRFMTRWQGDP
DPKHVEAIDAYWVSAAEHGMNASTFTARVIASTGADVAASLSGAVGAMSGPLHGGAPARVLPMIEQAEKTGDARGVIRGI
LDRREKLMGFGHRVYRAEDPRARVLRSTAKRLGAARYEVAAALEQAALAELRERRPDRVIETNVEFWAAVILDFAQVPTR
MMPAMFTCARTAGWSAHIMEQKRLGKLVRPAALYVGPGPRPIESVEGFGLLHSRVGA

Specific function: Catalyzes The Synthesis Of 2-Methylcitrate From Propionyl-CoA And Oxaloacetate. Also Catalyzes The Condensation Of Oxaloacetate With Acetyl-CoA But With A Lower Specificity. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=379, Percent_Identity=33.245382585752, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1786939, Length=207, Percent_Identity=36.7149758454106, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 40507; Mature: 40376

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAAPLPADFAPGLEGVTAFATEIAEPDKDGGSLRYRGVDIEDLVAQRVTFGDVWALLV
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHCCHHHHEEEEE
DGKFGQGLPPAEPFPIPVHTGDVRVDVQAGLAMLAPIWGFEPLLDTEDSVARSQLARASV
CCCCCCCCCCCCCCCCEEECCCEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
MALSYVAQSARGNSLPAVPQHIVDECSTVTSRFMTRWQGDPDPKHVEAIDAYWVSAAEHG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
MNASTFTARVIASTGADVAASLSGAVGAMSGPLHGGAPARVLPMIEQAEKTGDARGVIRG
CCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
ILDRREKLMGFGHRVYRAEDPRARVLRSTAKRLGAARYEVAAALEQAALAELRERRPDRV
HHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IETNVEFWAAVILDFAQVPTRMMPAMFTCARTAGWSAHIMEQKRLGKLVRPAALYVGPGP
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCC
RPIESVEGFGLLHSRVGA
CCHHHHCCHHHHHHHCCC
>Mature Secondary Structure 
TVAAPLPADFAPGLEGVTAFATEIAEPDKDGGSLRYRGVDIEDLVAQRVTFGDVWALLV
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHCCHHHHEEEEE
DGKFGQGLPPAEPFPIPVHTGDVRVDVQAGLAMLAPIWGFEPLLDTEDSVARSQLARASV
CCCCCCCCCCCCCCCCEEECCCEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
MALSYVAQSARGNSLPAVPQHIVDECSTVTSRFMTRWQGDPDPKHVEAIDAYWVSAAEHG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
MNASTFTARVIASTGADVAASLSGAVGAMSGPLHGGAPARVLPMIEQAEKTGDARGVIRG
CCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH
ILDRREKLMGFGHRVYRAEDPRARVLRSTAKRLGAARYEVAAALEQAALAELRERRPDRV
HHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IETNVEFWAAVILDFAQVPTRMMPAMFTCARTAGWSAHIMEQKRLGKLVRPAALYVGPGP
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCC
RPIESVEGFGLLHSRVGA
CCHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]