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The map label for this gene is pdhC [H]

Identifier: 169627994

GI number: 169627994

Start: 887998

End: 889233

Strand: Reverse

Name: pdhC [H]

Synonym: MAB_0895c

Alternate gene names: 169627994

Gene position: 889233-887998 (Counterclockwise)

Preceding gene: 169627995

Following gene: 169627993

Centisome position: 17.55

GC content: 62.86

Gene sequence:

>1236_bases
ATGACCGCTCAGACATTCAACCTGCCCGACCTCGGCGAAGGTCTCACGGAAGCGATCATCATCAAATGGCTAGTGTCGGT
CGGCGACACCGTCGTCATCGATCAGCCGGTCGTCGAAGTCGAAACTGCCAAAGCTTCCGTCGAGGTACCGGTTCCCTTCG
CCGGAACCATTGTCGAGCTCCACGGCGATCCCGGGGCCGCGATGCCAGTCGGATCGCCGTTGATCACGGTGCGCGGCAAC
GACTCGACTGATCCGGGCGACTCGCCCAGCGCTGACGCGTTTGCACAACACCGCGAGGAAGAACGCGCCGGATCGGGCAA
TGTACTCATCGGGTACGGCACCAGCACCGATGAAGGCCCCACGCGCAGACGGAAGCGTACGCACGAGCCGGCTGTCGGCA
CCGTTACGAAGGTCATTTCGCCGTTAGTGCGAAAGCTTGCTGCCGACAAGGGAATCAACCTGGCACAGGCGTCGGGTACC
GGCGCCGGAGGCGTCATCACCCGCTCCGACGTCGAACGGCTGGTTTCGACAAACCCGCCAGCCGGTGCAGGCAACGTCAC
TCGCATCCCGGTTACCGGCATTCGAAAGGTCATCGCCGACAAGCTCAGTATCAGCCGTCGCGAGATACCCGAGGCAACCG
TCTGGGTTGATGTCGACGCGACGGAACTGCTCGCCGCCAAACGTCTGCTCAGCCAGACGCAGTCGATCAGCCTGCTGGCA
CTCCTGGCCCGACTGACCGTCGCCGCACTCAAACAGTTTCCAGAGTTGAACGCCACATTCGATACCGGGCGCCAAGAAAT
CCTCCAGTACAGCGATATACACCTCGGAGTGGCGGTTCAGGCGCCATACGGTTTGGTGGTTCCCGTCATCTCGCAGGCGG
ACACGCTCGACACAGTGGCCTTGTCAGATGCCCTCGCCGAAGTCATTGCGCTGGCCCGCGACAGCAAGCTGCCCCCAGCC
AAGATGTCCGGGGGCACAGTGACGTTGAACAACTACGGCGTCTTCGGGGTCGACGGGTCGGCGGCGATCATCAATCACCC
CGAAGCCGCCATCCTGGGGGTCGGCCGCATCATTGACCGGCCATGGGTGGTCGACGGTCAGCTGACCGTGCGAAAGACCA
TGGAGCTGACGTTGAGTTTTGACCACCGGGTGTGCGACGGCGGCTCTGCCGCCGGTTTCCTGCGCCTCTTTGCCGACTAC
GTAGAGAATCCCGTCGCTGCGCTGGGCCGACTGTGA

Upstream 100 bases:

>100_bases
GGCTCGAGCGATGGTATCTGCCCAGTACCGACCGCATCCTCGACGCGGTCGATCGCCTGCAATGGGACGACCACCCCGAT
AGCCGTTGGGTGGTCAGCGC

Downstream 100 bases:

>100_bases
ACGCGGCACAGCACGCGGACGTGCTCATCCTCGGGGGCGGCTCGGCGGGTTACTCCTGCGCGCTGCGCGCCAGCCAACTC
GGCATGTCGGTCACACTCAT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTAQTFNLPDLGEGLTEAIIIKWLVSVGDTVVIDQPVVEVETAKASVEVPVPFAGTIVELHGDPGAAMPVGSPLITVRGN
DSTDPGDSPSADAFAQHREEERAGSGNVLIGYGTSTDEGPTRRRKRTHEPAVGTVTKVISPLVRKLAADKGINLAQASGT
GAGGVITRSDVERLVSTNPPAGAGNVTRIPVTGIRKVIADKLSISRREIPEATVWVDVDATELLAAKRLLSQTQSISLLA
LLARLTVAALKQFPELNATFDTGRQEILQYSDIHLGVAVQAPYGLVVPVISQADTLDTVALSDALAEVIALARDSKLPPA
KMSGGTVTLNNYGVFGVDGSAAIINHPEAAILGVGRIIDRPWVVDGQLTVRKTMELTLSFDHRVCDGGSAAGFLRLFADY
VENPVAALGRL

Sequences:

>Translated_411_residues
MTAQTFNLPDLGEGLTEAIIIKWLVSVGDTVVIDQPVVEVETAKASVEVPVPFAGTIVELHGDPGAAMPVGSPLITVRGN
DSTDPGDSPSADAFAQHREEERAGSGNVLIGYGTSTDEGPTRRRKRTHEPAVGTVTKVISPLVRKLAADKGINLAQASGT
GAGGVITRSDVERLVSTNPPAGAGNVTRIPVTGIRKVIADKLSISRREIPEATVWVDVDATELLAAKRLLSQTQSISLLA
LLARLTVAALKQFPELNATFDTGRQEILQYSDIHLGVAVQAPYGLVVPVISQADTLDTVALSDALAEVIALARDSKLPPA
KMSGGTVTLNNYGVFGVDGSAAIINHPEAAILGVGRIIDRPWVVDGQLTVRKTMELTLSFDHRVCDGGSAAGFLRLFADY
VENPVAALGRL
>Mature_410_residues
TAQTFNLPDLGEGLTEAIIIKWLVSVGDTVVIDQPVVEVETAKASVEVPVPFAGTIVELHGDPGAAMPVGSPLITVRGND
STDPGDSPSADAFAQHREEERAGSGNVLIGYGTSTDEGPTRRRKRTHEPAVGTVTKVISPLVRKLAADKGINLAQASGTG
AGGVITRSDVERLVSTNPPAGAGNVTRIPVTGIRKVIADKLSISRREIPEATVWVDVDATELLAAKRLLSQTQSISLLAL
LARLTVAALKQFPELNATFDTGRQEILQYSDIHLGVAVQAPYGLVVPVISQADTLDTVALSDALAEVIALARDSKLPPAK
MSGGTVTLNNYGVFGVDGSAAIINHPEAAILGVGRIIDRPWVVDGQLTVRKTMELTLSFDHRVCDGGSAAGFLRLFADYV
ENPVAALGRL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=434, Percent_Identity=29.9539170506912, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI110671329, Length=440, Percent_Identity=26.5909090909091, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI203098753, Length=456, Percent_Identity=26.0964912280702, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI203098816, Length=456, Percent_Identity=26.3157894736842, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=32.4786324786325, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI260898739, Length=166, Percent_Identity=34.3373493975904, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1786946, Length=429, Percent_Identity=29.1375291375291, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1786305, Length=303, Percent_Identity=30.6930693069307, Blast_Score=130, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25146366, Length=408, Percent_Identity=30.3921568627451, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=430, Percent_Identity=28.6046511627907, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17537937, Length=441, Percent_Identity=22.9024943310658, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=218, Percent_Identity=34.4036697247706, Blast_Score=124, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=412, Percent_Identity=26.9417475728155, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324258, Length=457, Percent_Identity=24.507658643326, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI18859875, Length=434, Percent_Identity=26.2672811059908, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24582497, Length=285, Percent_Identity=32.6315789473684, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI20129315, Length=285, Percent_Identity=32.6315789473684, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24645909, Length=239, Percent_Identity=31.7991631799163, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43148; Mature: 43016

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQTFNLPDLGEGLTEAIIIKWLVSVGDTVVIDQPVVEVETAKASVEVPVPFAGTIVEL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCEEEECCCCCCEEEEE
HGDPGAAMPVGSPLITVRGNDSTDPGDSPSADAFAQHREEERAGSGNVLIGYGTSTDEGP
ECCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
TRRRKRTHEPAVGTVTKVISPLVRKLAADKGINLAQASGTGAGGVITRSDVERLVSTNPP
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHCCCC
AGAGNVTRIPVTGIRKVIADKLSISRREIPEATVWVDVDATELLAAKRLLSQTQSISLLA
CCCCCEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHH
LLARLTVAALKQFPELNATFDTGRQEILQYSDIHLGVAVQAPYGLVVPVISQADTLDTVA
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEHHHCCCCHHHHHH
LSDALAEVIALARDSKLPPAKMSGGTVTLNNYGVFGVDGSAAIINHPEAAILGVGRIIDR
HHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEECCCCEEEEECCCHHEEEHHHHHCC
PWVVDGQLTVRKTMELTLSFDHRVCDGGSAAGFLRLFADYVENPVAALGRL
CEEECCCEEEEEEEEEEEEECCEECCCCCHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure 
TAQTFNLPDLGEGLTEAIIIKWLVSVGDTVVIDQPVVEVETAKASVEVPVPFAGTIVEL
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEEECCCEEEECCCCCCEEEEE
HGDPGAAMPVGSPLITVRGNDSTDPGDSPSADAFAQHREEERAGSGNVLIGYGTSTDEGP
ECCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCC
TRRRKRTHEPAVGTVTKVISPLVRKLAADKGINLAQASGTGAGGVITRSDVERLVSTNPP
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHCCCC
AGAGNVTRIPVTGIRKVIADKLSISRREIPEATVWVDVDATELLAAKRLLSQTQSISLLA
CCCCCEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHH
LLARLTVAALKQFPELNATFDTGRQEILQYSDIHLGVAVQAPYGLVVPVISQADTLDTVA
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEHHHCCCCHHHHHH
LSDALAEVIALARDSKLPPAKMSGGTVTLNNYGVFGVDGSAAIINHPEAAILGVGRIIDR
HHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEECCCCEEEEECCCHHEEEHHHHHCC
PWVVDGQLTVRKTMELTLSFDHRVCDGGSAAGFLRLFADYVENPVAALGRL
CEEECCCEEEEEEEEEEEEECCEECCCCCHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA