Click here to switch to the map view.

The map label for this gene is pdhD [H]

Identifier: 169627993

GI number: 169627993

Start: 886622

End: 888001

Strand: Reverse

Name: pdhD [H]

Synonym: MAB_0894c

Alternate gene names: 169627993

Gene position: 888001-886622 (Counterclockwise)

Preceding gene: 169627994

Following gene: 169627991

Centisome position: 17.52

GC content: 61.74

Gene sequence:

>1380_bases
GTGAACGCGGCACAGCACGCGGACGTGCTCATCCTCGGGGGCGGCTCGGCGGGTTACTCCTGCGCGCTGCGCGCCAGCCA
ACTCGGCATGTCGGTCACACTCATCGAAGCAGACAAACTCGGTGGCACCTGCCTGCACAGGGGGTGCATTCCTACCAAAG
CACTGCTGCATACTGCTGAACTTGCCGACAATTCCCGCACTGCAGCAGATTTCGGTATTCGGACACGCTTCGATGGCGTC
GATATGACCGCCGTACATGCCTACAAGCAGTCCGTGGTTTCACGACTACACAAAGGACTCGAAGGGCTGGTTGCCAATCG
AAAGATCACTGTCGTGCACGGTGTTGGCCGCTATCTCGGACCCCGCTCAGTGGACGTCGACGGCACTGTCTACACCGGCG
ATGCCGTGGTGCTCGCAACCGGTTCCGCACCGCGCAACATTCCAGCCATCCCTACGAGCTTCCGCATCGTCACCAGCGAC
CATGCTCTGGAACTGTCTTACGTCCCCGAGAGGGCAATTGTCCTGGGCGGCGGGGTGATCGGCGTAGAGTTCGCGAGCCT
ATGGACCTCGTTGGGATCGTCGGTAACCATCGTTGAATCCCTGCCGCGACTATTGGCCGGGGAAGACAACTGGGCATCGG
AAACCCTCGAACGCTCCTTGCGCCGTCGAGGAATAGCGGTCAAAACCAGCAGCATGGTGACCGACGCCGCCGATACCGGC
GACGGCGTCATCGTGACACTCGAAGGCGGCCAGACCCTGACCGCAGACGTACTTCTGGTCGCGGTGGGCCGTCAGCCACA
ATCCCAGGCCTTAGCGGATGCCGGAATCGACGTGGACCGAGGATTCGTGCGTGTCGATGACCGACTTGCCACGAATCATG
CGGGTGTCTACGCCGTCGGCGATCTCGTTGCGGGCCAACAACTCGCGCACCGAGGCTTCGCCCACGGGATCCTCGTCGCC
GAGGTCATCGCAAAAGGCGATCCAGTACCCGTGGCCGAGCACCTCATCCCCCGAGTCACCTACTCGCATCCCGAGGTGGC
TTCCGTCGGCCTCACCGAGGCAGCCGCACGCGAAGAATACGGCGAGATCACCACGACCGTGTATGACCTAGCCGGCAACG
GCAAGAGCCAAATACTGCGAACTTCCGGTGGAATCAAAGTGATTCGCCGTGGACCCGCCGATGGGGACGGCGCGATTGTG
GGAATCCACTGCGTCGGCGATCGCGTCGGCGAACTCATCGGTGAAGCTCAACTCATGGTCGGCTGGGAAGCGCTTCCCAC
CGAGGTTCGACCGTTTATGCACGCTCATCCGACGCAGAATGAAGCGCTTGGTGAAGCCCTGCTTGCGCTCGCAGGTACAC
CGCTGCATAAACACTCCTGA

Upstream 100 bases:

>100_bases
TGAGTTTTGACCACCGGGTGTGCGACGGCGGCTCTGCCGCCGGTTTCCTGCGCCTCTTTGCCGACTACGTAGAGAATCCC
GTCGCTGCGCTGGGCCGACT

Downstream 100 bases:

>100_bases
CTGATATCGGTCAGAAACATCATTCGCGAGCAGTCACCCGCAAAGCGGGCGGACATTGTCATTGAATTGGCGTAGACGCA
ACCCCGACCGGTGATCGGTC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; S complex, 50 kDa subunit [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MNAAQHADVLILGGGSAGYSCALRASQLGMSVTLIEADKLGGTCLHRGCIPTKALLHTAELADNSRTAADFGIRTRFDGV
DMTAVHAYKQSVVSRLHKGLEGLVANRKITVVHGVGRYLGPRSVDVDGTVYTGDAVVLATGSAPRNIPAIPTSFRIVTSD
HALELSYVPERAIVLGGGVIGVEFASLWTSLGSSVTIVESLPRLLAGEDNWASETLERSLRRRGIAVKTSSMVTDAADTG
DGVIVTLEGGQTLTADVLLVAVGRQPQSQALADAGIDVDRGFVRVDDRLATNHAGVYAVGDLVAGQQLAHRGFAHGILVA
EVIAKGDPVPVAEHLIPRVTYSHPEVASVGLTEAAAREEYGEITTTVYDLAGNGKSQILRTSGGIKVIRRGPADGDGAIV
GIHCVGDRVGELIGEAQLMVGWEALPTEVRPFMHAHPTQNEALGEALLALAGTPLHKHS

Sequences:

>Translated_459_residues
MNAAQHADVLILGGGSAGYSCALRASQLGMSVTLIEADKLGGTCLHRGCIPTKALLHTAELADNSRTAADFGIRTRFDGV
DMTAVHAYKQSVVSRLHKGLEGLVANRKITVVHGVGRYLGPRSVDVDGTVYTGDAVVLATGSAPRNIPAIPTSFRIVTSD
HALELSYVPERAIVLGGGVIGVEFASLWTSLGSSVTIVESLPRLLAGEDNWASETLERSLRRRGIAVKTSSMVTDAADTG
DGVIVTLEGGQTLTADVLLVAVGRQPQSQALADAGIDVDRGFVRVDDRLATNHAGVYAVGDLVAGQQLAHRGFAHGILVA
EVIAKGDPVPVAEHLIPRVTYSHPEVASVGLTEAAAREEYGEITTTVYDLAGNGKSQILRTSGGIKVIRRGPADGDGAIV
GIHCVGDRVGELIGEAQLMVGWEALPTEVRPFMHAHPTQNEALGEALLALAGTPLHKHS
>Mature_459_residues
MNAAQHADVLILGGGSAGYSCALRASQLGMSVTLIEADKLGGTCLHRGCIPTKALLHTAELADNSRTAADFGIRTRFDGV
DMTAVHAYKQSVVSRLHKGLEGLVANRKITVVHGVGRYLGPRSVDVDGTVYTGDAVVLATGSAPRNIPAIPTSFRIVTSD
HALELSYVPERAIVLGGGVIGVEFASLWTSLGSSVTIVESLPRLLAGEDNWASETLERSLRRRGIAVKTSSMVTDAADTG
DGVIVTLEGGQTLTADVLLVAVGRQPQSQALADAGIDVDRGFVRVDDRLATNHAGVYAVGDLVAGQQLAHRGFAHGILVA
EVIAKGDPVPVAEHLIPRVTYSHPEVASVGLTEAAAREEYGEITTTVYDLAGNGKSQILRTSGGIKVIRRGPADGDGAIV
GIHCVGDRVGELIGEAQLMVGWEALPTEVRPFMHAHPTQNEALGEALLALAGTPLHKHS

Specific function: Catalyzes the oxidation of dihydrolipoamide to lipoamide [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=468, Percent_Identity=36.5384615384615, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=28.9416846652268, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI148277065, Length=361, Percent_Identity=24.3767313019391, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI148277071, Length=361, Percent_Identity=24.3767313019391, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI33519430, Length=361, Percent_Identity=24.3767313019391, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI33519428, Length=361, Percent_Identity=24.3767313019391, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI33519426, Length=361, Percent_Identity=24.3767313019391, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI22035672, Length=471, Percent_Identity=25.6900212314225, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI291045266, Length=383, Percent_Identity=25.5874673629243, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI291045268, Length=338, Percent_Identity=26.3313609467456, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1786307, Length=467, Percent_Identity=35.117773019272, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1789915, Length=429, Percent_Identity=29.6037296037296, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87082354, Length=458, Percent_Identity=27.9475982532751, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=29.2841648590022, Blast_Score=131, Evalue=8e-32,
Organism=Escherichia coli, GI1789065, Length=215, Percent_Identity=28.3720930232558, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788892, Length=187, Percent_Identity=29.9465240641711, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=468, Percent_Identity=35.6837606837607, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI71983429, Length=385, Percent_Identity=29.0909090909091, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71983419, Length=385, Percent_Identity=29.0909090909091, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17557007, Length=446, Percent_Identity=27.5784753363229, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=399, Percent_Identity=24.812030075188, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17559934, Length=137, Percent_Identity=28.4671532846715, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=475, Percent_Identity=34.3157894736842, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=27.8481012658228, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6325166, Length=476, Percent_Identity=26.6806722689076, Blast_Score=136, Evalue=8e-33,
Organism=Drosophila melanogaster, GI21358499, Length=475, Percent_Identity=33.6842105263158, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24640553, Length=450, Percent_Identity=27.1111111111111, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24640549, Length=444, Percent_Identity=27.2522522522523, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24640551, Length=437, Percent_Identity=27.2311212814645, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI17737741, Length=443, Percent_Identity=24.8306997742664, Blast_Score=99, Evalue=6e-21,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 47966; Mature: 47966

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAQHADVLILGGGSAGYSCALRASQLGMSVTLIEADKLGGTCLHRGCIPTKALLHTAE
CCCCCCCCEEEEECCCCCCEEEEEHHHCCCEEEEEEECCCCCHHHHCCCCCHHHHHHHHH
LADNSRTAADFGIRTRFDGVDMTAVHAYKQSVVSRLHKGLEGLVANRKITVVHGVGRYLG
HCCCCCCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHCC
PRSVDVDGTVYTGDAVVLATGSAPRNIPAIPTSFRIVTSDHALELSYVPERAIVLGGGVI
CCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCEEEEECCCEEEEEECCCEEEEEECCCH
GVEFASLWTSLGSSVTIVESLPRLLAGEDNWASETLERSLRRRGIAVKTSSMVTDAADTG
HHHHHHHHHHHCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCEECCCCCC
DGVIVTLEGGQTLTADVLLVAVGRQPQSQALADAGIDVDRGFVRVDDRLATNHAGVYAVG
CCEEEEECCCCEEEEEEEEEEECCCCHHHHHHHCCCCCCCCEEEECCCHHCCCCCEEEEH
DLVAGQQLAHRGFAHGILVAEVIAKGDPVPVAEHLIPRVTYSHPEVASVGLTEAAAREEY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHCCCHHHHHHHHH
GEITTTVYDLAGNGKSQILRTSGGIKVIRRGPADGDGAIVGIHCVGDRVGELIGEAQLMV
CCCEEEEEEECCCCHHHEEEECCCEEEEEECCCCCCCCEEEEEECCHHHHHHHCCCEEEE
GWEALPTEVRPFMHAHPTQNEALGEALLALAGTPLHKHS
CCHHCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNAAQHADVLILGGGSAGYSCALRASQLGMSVTLIEADKLGGTCLHRGCIPTKALLHTAE
CCCCCCCCEEEEECCCCCCEEEEEHHHCCCEEEEEEECCCCCHHHHCCCCCHHHHHHHHH
LADNSRTAADFGIRTRFDGVDMTAVHAYKQSVVSRLHKGLEGLVANRKITVVHGVGRYLG
HCCCCCCHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHCC
PRSVDVDGTVYTGDAVVLATGSAPRNIPAIPTSFRIVTSDHALELSYVPERAIVLGGGVI
CCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCEEEEECCCEEEEEECCCEEEEEECCCH
GVEFASLWTSLGSSVTIVESLPRLLAGEDNWASETLERSLRRRGIAVKTSSMVTDAADTG
HHHHHHHHHHHCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCEECCCCCC
DGVIVTLEGGQTLTADVLLVAVGRQPQSQALADAGIDVDRGFVRVDDRLATNHAGVYAVG
CCEEEEECCCCEEEEEEEEEEECCCCHHHHHHHCCCCCCCCEEEECCCHHCCCCCEEEEH
DLVAGQQLAHRGFAHGILVAEVIAKGDPVPVAEHLIPRVTYSHPEVASVGLTEAAAREEY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHCCCHHHHHHHHH
GEITTTVYDLAGNGKSQILRTSGGIKVIRRGPADGDGAIVGIHCVGDRVGELIGEAQLMV
CCCEEEEEEECCCCHHHEEEECCCEEEEEECCCCCCCCEEEEEECCHHHHHHHCCCEEEE
GWEALPTEVRPFMHAHPTQNEALGEALLALAGTPLHKHS
CCHHCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 1936936 [H]