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The map label for this gene is nemA [H]

Identifier: 169627928

GI number: 169627928

Start: 825982

End: 826461

Strand: Direct

Name: nemA [H]

Synonym: MAB_0829

Alternate gene names: 169627928

Gene position: 825982-826461 (Clockwise)

Preceding gene: 169627927

Following gene: 169627929

Centisome position: 16.3

GC content: 50.62

Gene sequence:

>480_bases
GTGGAGAATCGACAGAGATTTGTGATTGAAGTTGCGCGACGTGTGGCAGAGGAAATTGGGCCCGAACGGACAGGGATTCG
TCTCTCGCCCGGAGGTGAGATAGGTGAAATTGAAGAGGGGGACAACTATGTCGACGAGTACATTCAACTTGCGGAAAAGC
TATCTGAACTATCGCTGGTATATATTCACATAGCGTTTCTACGGCCTACTACGGATAAAGATGATCAACTGTTGCGTCGG
CTTCGTGAGGCTTATGATGGTGTAGTGATCGTCAATAGAGGTGGACGTCCTGCCGAATATATTGGCCAAGATGTTTCGAC
TGGTTTAGCTGACATCGAATCCATTGGAGCGCTGGCGTTAGCAAATCCGGATTTGCCTCGACGGCTGGAAATGGAACTAC
CGCTGAACGAGCCGCGGAAGGAATTCTTCTACGGCGGCGGACGTGAGGGGTATACGGACTATCCGGTTTACCTACCGTGA

Upstream 100 bases:

>100_bases
AGGGGCGGACGGTGTGGAATTGCACGGTGCAAATGGGTATCTACTGCAGCAGTTCTTGGCTCCGAACACTAATACGCGCA
CTGATGGTTACGGTGGAAGC

Downstream 100 bases:

>100_bases
GTCAGGCTAAGAATGTTTCGGATGTACACATCGGCTGCCTAGTTATTTGTGACATCTAGGATTAGAAGACTCAAAAGCTA
AATATCAATATCAGATGTGA

Product: putative oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MENRQRFVIEVARRVAEEIGPERTGIRLSPGGEIGEIEEGDNYVDEYIQLAEKLSELSLVYIHIAFLRPTTDKDDQLLRR
LREAYDGVVIVNRGGRPAEYIGQDVSTGLADIESIGALALANPDLPRRLEMELPLNEPRKEFFYGGGREGYTDYPVYLP

Sequences:

>Translated_159_residues
MENRQRFVIEVARRVAEEIGPERTGIRLSPGGEIGEIEEGDNYVDEYIQLAEKLSELSLVYIHIAFLRPTTDKDDQLLRR
LREAYDGVVIVNRGGRPAEYIGQDVSTGLADIESIGALALANPDLPRRLEMELPLNEPRKEFFYGGGREGYTDYPVYLP
>Mature_159_residues
MENRQRFVIEVARRVAEEIGPERTGIRLSPGGEIGEIEEGDNYVDEYIQLAEKLSELSLVYIHIAFLRPTTDKDDQLLRR
LREAYDGVVIVNRGGRPAEYIGQDVSTGLADIESIGALALANPDLPRRLEMELPLNEPRKEFFYGGGREGYTDYPVYLP

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=161, Percent_Identity=37.2670807453416, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325086, Length=178, Percent_Identity=33.1460674157303, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 17984; Mature: 17984

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENRQRFVIEVARRVAEEIGPERTGIRLSPGGEIGEIEEGDNYVDEYIQLAEKLSELSLV
CCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEE
YIHIAFLRPTTDKDDQLLRRLREAYDGVVIVNRGGRPAEYIGQDVSTGLADIESIGALAL
EEEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHCCHHHHHHHHHHHHCEEEE
ANPDLPRRLEMELPLNEPRKEFFYGGGREGYTDYPVYLP
CCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MENRQRFVIEVARRVAEEIGPERTGIRLSPGGEIGEIEEGDNYVDEYIQLAEKLSELSLV
CCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEE
YIHIAFLRPTTDKDDQLLRRLREAYDGVVIVNRGGRPAEYIGQDVSTGLADIESIGALAL
EEEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHCCHHHHHHHHHHHHCEEEE
ANPDLPRRLEMELPLNEPRKEFFYGGGREGYTDYPVYLP
CCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]