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The map label for this gene is nemA [H]

Identifier: 169627929

GI number: 169627929

Start: 826921

End: 828015

Strand: Direct

Name: nemA [H]

Synonym: MAB_0830

Alternate gene names: 169627929

Gene position: 826921-828015 (Clockwise)

Preceding gene: 169627928

Following gene: 169627930

Centisome position: 16.32

GC content: 57.99

Gene sequence:

>1095_bases
ATGGCATTTACCCTGGACCCACAAGCTGCTCTCCTCAAACCGATTCGTATGGGCGAGGTTGTTGCGTCCAATCGAATCTT
GATGGCGCCGATGACGCGTTCGCGGGCACAAGGAGATGGCACCCCATCAGAGTTTGCGCCGGTCTATTACGGCCAGCGCG
CGAGCGCTGGCCTAATCATCAGCGAGGCCACTGCGATCTCCCAGGCTGGCAACGGCGCGTATATGAATACACCCGGCATC
TACACCGATCGACACCAACACAAGTGGGCTGAAATCGCCGAGACAGTGCATTCTGAGGGCAGCAAGATGTTCTTGCAAAT
CTGGCATGTGGGGCGGTTGGGCCACCCGGCGGTCAGTGGAGGTTTTGAGCCGGTTGCACCGTCGGCGGTTGCCGCCCAGA
TGACCACTCACACGCCTTCAGGCAAGGTGCCCTTAGTGATTCCACGGGAGTTGCGTATAGACGAGATCGCAGAGATCGTA
GACGATTTTCGTGCCGCTGCGCGCCGTGCCATAGACGCCGGTTTGGACGGTGTCGAGATACACGGCGCGAATGGGTATCT
ACTTCACCAGTTCTCGTCCGATGTCATCAACCAACGCACTGATATCTATGGCGGATCGCCGGAGAATAGGGCCCGTCTGA
CCGCCGAGGTAGTCGAGGCCGTTGCCGCAGAAGTTGGTCCTACCAGAGTGGGTGTGCGCATATCGCCGGGAACGAGCGCC
GGCGATATGCACGAGAATGACACCGTGTCCACATATGCGGCGTTACTGGAGCGTATTGACCCATTGGGTCTGGCCTACTT
ACATGTGGTCATCGATCCAGGTGCCGTAGAGCTCCGGGCCATGGGAGCTATGTGGTCGAACAATCTGGTCTTGAATTCCA
GCACGGAAACCGAGACGGAATTTTGCGTTCTAGAGGGGTTGGTCGAGTCAGGCGCTGTCAATGCAGTAGCCCTCGGGCGT
AAATTCCTCGCCAACCCAGATTTGATCGATCGGCTGCTGTTGGGCGCTGAACTTAACGAAGCGAACGCGGCCACCTTCTA
CAGCCCGGGCAAGGAAGGTTACATTGATTACCCCAGCCTCTCAGCGGGTCGCTAG

Upstream 100 bases:

>100_bases
AGGGGTTGTAGAGTATGTGTGCCGTAGTGGCGTTGCGGCAAACAGTGATGTATTAAAAAGTACGAAGGAAGAAAAGCAAG
CCAGGAAAGCTTATACGGAT

Downstream 100 bases:

>100_bases
GGTCAGATCATGGCCACTAGCGACACCCGGGAAATCGTCATCGAAGCGACTCCCGAACAGATCATGGATGTCATCGCCGA
CTTTGAGGTGCTGCCGAAAT

Product: NADH-dependent flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MAFTLDPQAALLKPIRMGEVVASNRILMAPMTRSRAQGDGTPSEFAPVYYGQRASAGLIISEATAISQAGNGAYMNTPGI
YTDRHQHKWAEIAETVHSEGSKMFLQIWHVGRLGHPAVSGGFEPVAPSAVAAQMTTHTPSGKVPLVIPRELRIDEIAEIV
DDFRAAARRAIDAGLDGVEIHGANGYLLHQFSSDVINQRTDIYGGSPENRARLTAEVVEAVAAEVGPTRVGVRISPGTSA
GDMHENDTVSTYAALLERIDPLGLAYLHVVIDPGAVELRAMGAMWSNNLVLNSSTETETEFCVLEGLVESGAVNAVALGR
KFLANPDLIDRLLLGAELNEANAATFYSPGKEGYIDYPSLSAGR

Sequences:

>Translated_364_residues
MAFTLDPQAALLKPIRMGEVVASNRILMAPMTRSRAQGDGTPSEFAPVYYGQRASAGLIISEATAISQAGNGAYMNTPGI
YTDRHQHKWAEIAETVHSEGSKMFLQIWHVGRLGHPAVSGGFEPVAPSAVAAQMTTHTPSGKVPLVIPRELRIDEIAEIV
DDFRAAARRAIDAGLDGVEIHGANGYLLHQFSSDVINQRTDIYGGSPENRARLTAEVVEAVAAEVGPTRVGVRISPGTSA
GDMHENDTVSTYAALLERIDPLGLAYLHVVIDPGAVELRAMGAMWSNNLVLNSSTETETEFCVLEGLVESGAVNAVALGR
KFLANPDLIDRLLLGAELNEANAATFYSPGKEGYIDYPSLSAGR
>Mature_363_residues
AFTLDPQAALLKPIRMGEVVASNRILMAPMTRSRAQGDGTPSEFAPVYYGQRASAGLIISEATAISQAGNGAYMNTPGIY
TDRHQHKWAEIAETVHSEGSKMFLQIWHVGRLGHPAVSGGFEPVAPSAVAAQMTTHTPSGKVPLVIPRELRIDEIAEIVD
DFRAAARRAIDAGLDGVEIHGANGYLLHQFSSDVINQRTDIYGGSPENRARLTAEVVEAVAAEVGPTRVGVRISPGTSAG
DMHENDTVSTYAALLERIDPLGLAYLHVVIDPGAVELRAMGAMWSNNLVLNSSTETETEFCVLEGLVESGAVNAVALGRK
FLANPDLIDRLLLGAELNEANAATFYSPGKEGYIDYPSLSAGR

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=368, Percent_Identity=42.3913043478261, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1789463, Length=223, Percent_Identity=34.9775784753363, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17559804, Length=383, Percent_Identity=27.6762402088773, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=350, Percent_Identity=27.7142857142857, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=383, Percent_Identity=26.3707571801567, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=246, Percent_Identity=30.0813008130081, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17565138, Length=351, Percent_Identity=27.6353276353276, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=353, Percent_Identity=26.628895184136, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325086, Length=376, Percent_Identity=35.1063829787234, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321973, Length=392, Percent_Identity=35.7142857142857, Blast_Score=186, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38818; Mature: 38687

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFTLDPQAALLKPIRMGEVVASNRILMAPMTRSRAQGDGTPSEFAPVYYGQRASAGLII
CCEECCCHHHHHHHHHHHHHHHCCEEEECCCCHHHCCCCCCCHHCCCEEECCCCCCCEEE
SEATAISQAGNGAYMNTPGIYTDRHQHKWAEIAETVHSEGSKMFLQIWHVGRLGHPAVSG
ECHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCC
GFEPVAPSAVAAQMTTHTPSGKVPLVIPRELRIDEIAEIVDDFRAAARRAIDAGLDGVEI
CCCCCCCHHHHHHHEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HGANGYLLHQFSSDVINQRTDIYGGSPENRARLTAEVVEAVAAEVGPTRVGVRISPGTSA
ECCCCEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GDMHENDTVSTYAALLERIDPLGLAYLHVVIDPGAVELRAMGAMWSNNLVLNSSTETETE
CCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEHHHHHHHCCCEEEECCCCCHHH
FCVLEGLVESGAVNAVALGRKFLANPDLIDRLLLGAELNEANAATFYSPGKEGYIDYPSL
HHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCC
SAGR
CCCC
>Mature Secondary Structure 
AFTLDPQAALLKPIRMGEVVASNRILMAPMTRSRAQGDGTPSEFAPVYYGQRASAGLII
CEECCCHHHHHHHHHHHHHHHCCEEEECCCCHHHCCCCCCCHHCCCEEECCCCCCCEEE
SEATAISQAGNGAYMNTPGIYTDRHQHKWAEIAETVHSEGSKMFLQIWHVGRLGHPAVSG
ECHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCC
GFEPVAPSAVAAQMTTHTPSGKVPLVIPRELRIDEIAEIVDDFRAAARRAIDAGLDGVEI
CCCCCCCHHHHHHHEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
HGANGYLLHQFSSDVINQRTDIYGGSPENRARLTAEVVEAVAAEVGPTRVGVRISPGTSA
ECCCCEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GDMHENDTVSTYAALLERIDPLGLAYLHVVIDPGAVELRAMGAMWSNNLVLNSSTETETE
CCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEHHHHHHHCCCEEEECCCCCHHH
FCVLEGLVESGAVNAVALGRKFLANPDLIDRLLLGAELNEANAATFYSPGKEGYIDYPSL
HHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCC
SAGR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]