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The map label for this gene is lvr [H]

Identifier: 169627793

GI number: 169627793

Start: 694332

End: 695153

Strand: Direct

Name: lvr [H]

Synonym: MAB_0691

Alternate gene names: 169627793

Gene position: 694332-695153 (Clockwise)

Preceding gene: 169627792

Following gene: 169627795

Centisome position: 13.7

GC content: 66.67

Gene sequence:

>822_bases
ATGCCCGATGAGGTCGTCGTCGTCATCGGCGTCGGCGGGATGGGCAGGGTCATCGCCCGTCGTCAGGGCATCGGCAAGGC
GCTGCTGCTCGCCGACCACAACGAGCAGGCGCTGCAGGCCGTCGTCGATGAGCTGAAGGCCGATGGACACAACGTCATCG
GGCAGCGCGTCGACGTCTCGTCGCGTGCTTCGGTTTCGGGGCTGGCCCAGGCCGCGGCGGGCCTGGGAAGGGTCGCTCAG
GTGGCGCACACCGCGGGGCTTTCGCCGGCGCAGTCGCCCACGGCCGCCATCTTGGCAGTAGACCTGGTCGGTGTGGCCTT
GGTGGTGGAGGAATTTGGCAAGATCGTCGCCTCCGGTGGCGCCGGTGTGGTGATTGCCAGCATGGCCGGACACATGCCGT
ACGAGCTCACCGCCGAGCAAGAACAAGAACTCGGCACCACCCACGCCGAGGACTTGCTCTCGCTGCCGTATGTACGGGAC
ATCGAGCATCCCGCAGTTGCGTACCAAGTGTCCAAGCGTGCCAATCACATTCGCGTTCGGTTCGCCGCCCGCCAGTGGGG
CACGCGGGGAGCCCGCATCAACTCGATAAGCCCCGGGGTGGTATCCACCCCGATGGGTCATCAGGAGCTCGCCTCCGAAA
CCGGAGCCATCACCAAGGCCATGGTCGACGGTTCGGCATCCAGACGGATCGGCACGTCCGACGACATCGCGGCGGCGGCG
GCTTTCCTGCTGGGCCCGGAGTCGTCCTATGTCACCGGCACAGATCTGTTGGTCGATGGCGGGGTCATCGCGGCGGTCCG
CTCCGGTCAGCTGTTCAACTGA

Upstream 100 bases:

>100_bases
GGGCACGGCGGGATAAGCGGCCGCTATGAACGCTGGAAGGAAAGCGCTTTCCAGCTCGCCTGGCTACTGGATATCCTTGG
AGCGCAGGGGGAGACGCCGA

Downstream 100 bases:

>100_bases
TGGCCGAGTCCATATCTTCCAGCGCCCTGCCCTTGGTCTCCTCTACCCAGCGCGCCACGAACAGCAGGGACAACACCGCG
CACAGCGCGTAGCACCCGTA

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MPDEVVVVIGVGGMGRVIARRQGIGKALLLADHNEQALQAVVDELKADGHNVIGQRVDVSSRASVSGLAQAAAGLGRVAQ
VAHTAGLSPAQSPTAAILAVDLVGVALVVEEFGKIVASGGAGVVIASMAGHMPYELTAEQEQELGTTHAEDLLSLPYVRD
IEHPAVAYQVSKRANHIRVRFAARQWGTRGARINSISPGVVSTPMGHQELASETGAITKAMVDGSASRRIGTSDDIAAAA
AFLLGPESSYVTGTDLLVDGGVIAAVRSGQLFN

Sequences:

>Translated_273_residues
MPDEVVVVIGVGGMGRVIARRQGIGKALLLADHNEQALQAVVDELKADGHNVIGQRVDVSSRASVSGLAQAAAGLGRVAQ
VAHTAGLSPAQSPTAAILAVDLVGVALVVEEFGKIVASGGAGVVIASMAGHMPYELTAEQEQELGTTHAEDLLSLPYVRD
IEHPAVAYQVSKRANHIRVRFAARQWGTRGARINSISPGVVSTPMGHQELASETGAITKAMVDGSASRRIGTSDDIAAAA
AFLLGPESSYVTGTDLLVDGGVIAAVRSGQLFN
>Mature_272_residues
PDEVVVVIGVGGMGRVIARRQGIGKALLLADHNEQALQAVVDELKADGHNVIGQRVDVSSRASVSGLAQAAAGLGRVAQV
AHTAGLSPAQSPTAAILAVDLVGVALVVEEFGKIVASGGAGVVIASMAGHMPYELTAEQEQELGTTHAEDLLSLPYVRDI
EHPAVAYQVSKRANHIRVRFAARQWGTRGARINSISPGVVSTPMGHQELASETGAITKAMVDGSASRRIGTSDDIAAAAA
FLLGPESSYVTGTDLLVDGGVIAAVRSGQLFN

Specific function: Catalyzes the regio- and stereoselective reversible NAD- dependent reduction of (6R)-2,2,6-trimethyl-1,4-cyclohexanedione (levodione) to (4R,6R)-4-hydroxy-2,2,6-trimethylcyclohexanone (actinol) [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 28051; Mature: 27920

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDEVVVVIGVGGMGRVIARRQGIGKALLLADHNEQALQAVVDELKADGHNVIGQRVDVS
CCCCEEEEEECCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHCCCCCCCC
SRASVSGLAQAAAGLGRVAQVAHTAGLSPAQSPTAAILAVDLVGVALVVEEFGKIVASGG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGVVIASMAGHMPYELTAEQEQELGTTHAEDLLSLPYVRDIEHPAVAYQVSKRANHIRVR
CCEEEEEHHCCCCEEECCHHHHHCCCHHHHHHHHCCCHHCCCCCHHHHHHHHCCCEEEEE
FAARQWGTRGARINSISPGVVSTPMGHQELASETGAITKAMVDGSASRRIGTSDDIAAAA
EEHHHCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
AFLLGPESSYVTGTDLLVDGGVIAAVRSGQLFN
HHHCCCCCCCEECCCEEECCCEEEEEECCCCCC
>Mature Secondary Structure 
PDEVVVVIGVGGMGRVIARRQGIGKALLLADHNEQALQAVVDELKADGHNVIGQRVDVS
CCCEEEEEECCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHCCCCCCCC
SRASVSGLAQAAAGLGRVAQVAHTAGLSPAQSPTAAILAVDLVGVALVVEEFGKIVASGG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
AGVVIASMAGHMPYELTAEQEQELGTTHAEDLLSLPYVRDIEHPAVAYQVSKRANHIRVR
CCEEEEEHHCCCCEEECCHHHHHCCCHHHHHHHHCCCHHCCCCCHHHHHHHHCCCEEEEE
FAARQWGTRGARINSISPGVVSTPMGHQELASETGAITKAMVDGSASRRIGTSDDIAAAA
EEHHHCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
AFLLGPESSYVTGTDLLVDGGVIAAVRSGQLFN
HHHCCCCCCCEECCCEEECCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11388460; 10508066 [H]