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The map label for this gene is purB [H]

Identifier: 169627789

GI number: 169627789

Start: 689144

End: 690562

Strand: Direct

Name: purB [H]

Synonym: MAB_0687

Alternate gene names: 169627789

Gene position: 689144-690562 (Clockwise)

Preceding gene: 169627788

Following gene: 169627791

Centisome position: 13.6

GC content: 66.17

Gene sequence:

>1419_bases
GTGCAAATCCCTAACGTTCTGGCCTCCCGGTACGCAAGCGACGCGATGACTGCGCTGTGGTCGCCCCAATCAAAGGTGGT
GCTGGAGCGCCAGTTGTGGATCGCGGTGCTGCGTGCACAACGCGATCTGGGCATCGAGGTTCCCGACGGTGTCATCGAGG
ACTACGAGCGTGTGGTCGAGAACGTGGACCTTGACTCGATCTCGGCACGTGAGCGCGTCACCCGCCACGATGTGAAGGCC
CGTATCGAGGAGTTCAACGCGCTCGCCGGACACGAGCACATCCATAAGGGCATGACGAGCCGCGATCTCACCGAGAACGT
GGAGCAACTGCAGATTCGCCGCTCGCTGGAGTACGTGCATGCCCATGGCGTCGCGGTGGTGGCGCGGCTCGCGCGTCACG
CCGTCGAGTACCGGGACGTGGTGATGGCCGGGCGCAGCCACAATGTCGCCGCGCAGGCAACCACGTTGGGTAAGCGGTTC
GCATCGGCCGCCGACGAGACTCTGCTCGCGCTGACCCGGTTGCGGGAGCTTCTCGACCGGTATCCGCTGCGGGGGATCAA
GGGGCCGATGGGCACCGGGCAGGACATGCTGGACCTGTTCAACGGAGACGTGGTCAAGCTGGCCGAGCTGGAGGAGCGTG
TTGCGGGCTTCCTCGGCTTTTCCACGACCCTGACGAGCGTGGGCCAGGTATACCCGCGCTCGCTGGACCACGATGTGGTG
TCGGCGTTGGTGCAACTCGGCGCCGGTCCGTCGTCGCTGGCGCACACCATCCGCCTGATGGCCGGACACGAGCTGGTCAC
CGAGGGCTTTGCGCCAGGACAGGTGGGCAGTTCGGCGATGCCGCACAAGATGAACACCCGCAGCTGTGAACGCGTCAACG
GCCTGCAGGTGATTTTGCGCGGCTACGGCTCGATGGCGGCGGAACTTGCTGGGGCGCAATGGAATGAAGGCGATGTGTTC
TGCTCGGTGGTGCGCCGTGTCGCGCTGCCCGACGCGTTCTTCGCGATCGACGGGATGATCGAGACCTTCTTGACGGTGCT
CGATGAGTTCGGTGCGTATCCGGCGGTGATCGACCGTGAGCTGCGCCGCTACCTGCCGTTCCTGGCGACCACCAAGGTGC
TCATGGCGGCGGTCCGGGCCGGAGTCGGCCGCGAGGAGGCCCACGAGGTGATCAAGGAACACGCGGTCGCGACGGCACTG
GCGATGCGTGAAAAGGGTGCCGAACCAAACCTTTTGGGTCTGCTGGCGGAGGATGCGCGACTGCCGCTGGACGCCGATGC
ACTGGAGGCGGCGCTGGCCGACCGCGCCTCGTTCACCGGTGCCGCCGCCGATCAAGTCGACCGGGTGGTTGCCGAGGTCG
AGGCGCTGGCGGCCGAGTATCCCGATGCCGCGGTGTACAGCCCGGGCGCCATTCTGTAG

Upstream 100 bases:

>100_bases
TGTACTAGCTCCGCTGGAAGCAGAACAGCTGGCTGAATCGGTTCCGGCCGATGAGCATCGTGGGCGAAGAGCATGGATCT
AGTCCCCATAGACTCTGGTT

Downstream 100 bases:

>100_bases
CTCACGCCCTCCCGAGGCGCCGACACGCCGTCTTCTGTTGCGAACCTGTGGCGTGTTGCCGCACAGTACGGCGTGTCGGC
GCGAAGGGGAGCGTTAGGCG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MQIPNVLASRYASDAMTALWSPQSKVVLERQLWIAVLRAQRDLGIEVPDGVIEDYERVVENVDLDSISARERVTRHDVKA
RIEEFNALAGHEHIHKGMTSRDLTENVEQLQIRRSLEYVHAHGVAVVARLARHAVEYRDVVMAGRSHNVAAQATTLGKRF
ASAADETLLALTRLRELLDRYPLRGIKGPMGTGQDMLDLFNGDVVKLAELEERVAGFLGFSTTLTSVGQVYPRSLDHDVV
SALVQLGAGPSSLAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGLQVILRGYGSMAAELAGAQWNEGDVF
CSVVRRVALPDAFFAIDGMIETFLTVLDEFGAYPAVIDRELRRYLPFLATTKVLMAAVRAGVGREEAHEVIKEHAVATAL
AMREKGAEPNLLGLLAEDARLPLDADALEAALADRASFTGAAADQVDRVVAEVEALAAEYPDAAVYSPGAIL

Sequences:

>Translated_472_residues
MQIPNVLASRYASDAMTALWSPQSKVVLERQLWIAVLRAQRDLGIEVPDGVIEDYERVVENVDLDSISARERVTRHDVKA
RIEEFNALAGHEHIHKGMTSRDLTENVEQLQIRRSLEYVHAHGVAVVARLARHAVEYRDVVMAGRSHNVAAQATTLGKRF
ASAADETLLALTRLRELLDRYPLRGIKGPMGTGQDMLDLFNGDVVKLAELEERVAGFLGFSTTLTSVGQVYPRSLDHDVV
SALVQLGAGPSSLAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGLQVILRGYGSMAAELAGAQWNEGDVF
CSVVRRVALPDAFFAIDGMIETFLTVLDEFGAYPAVIDRELRRYLPFLATTKVLMAAVRAGVGREEAHEVIKEHAVATAL
AMREKGAEPNLLGLLAEDARLPLDADALEAALADRASFTGAAADQVDRVVAEVEALAAEYPDAAVYSPGAIL
>Mature_472_residues
MQIPNVLASRYASDAMTALWSPQSKVVLERQLWIAVLRAQRDLGIEVPDGVIEDYERVVENVDLDSISARERVTRHDVKA
RIEEFNALAGHEHIHKGMTSRDLTENVEQLQIRRSLEYVHAHGVAVVARLARHAVEYRDVVMAGRSHNVAAQATTLGKRF
ASAADETLLALTRLRELLDRYPLRGIKGPMGTGQDMLDLFNGDVVKLAELEERVAGFLGFSTTLTSVGQVYPRSLDHDVV
SALVQLGAGPSSLAHTIRLMAGHELVTEGFAPGQVGSSAMPHKMNTRSCERVNGLQVILRGYGSMAAELAGAQWNEGDVF
CSVVRRVALPDAFFAIDGMIETFLTVLDEFGAYPAVIDRELRRYLPFLATTKVLMAAVRAGVGREEAHEVIKEHAVATAL
AMREKGAEPNLLGLLAEDARLPLDADALEAALADRASFTGAAADQVDRVVAEVEALAAEYPDAAVYSPGAIL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=448, Percent_Identity=33.4821428571429, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI183227688, Length=381, Percent_Identity=34.1207349081365, Blast_Score=211, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17508577, Length=476, Percent_Identity=29.2016806722689, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI32564234, Length=346, Percent_Identity=31.7919075144509, Blast_Score=162, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6323391, Length=452, Percent_Identity=36.0619469026549, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24647570, Length=460, Percent_Identity=33.9130434782609, Blast_Score=234, Evalue=9e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51288; Mature: 51288

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure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CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]