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The map label for this gene is purD [H]

Identifier: 169627784

GI number: 169627784

Start: 684178

End: 685446

Strand: Direct

Name: purD [H]

Synonym: MAB_0682

Alternate gene names: 169627784

Gene position: 684178-685446 (Clockwise)

Preceding gene: 169627783

Following gene: 169627787

Centisome position: 13.5

GC content: 68.72

Gene sequence:

>1269_bases
ATGGAGTCCTTGCGTGTCCTCATTATTGGATCCGGTGGCCGTGAACACGCTTTGGCGATCGCCCTGAAGCGAGACCCACG
CGTGGACTACCTGGCGATAGCGCCGGGCAATGCGGGAACCGCCGCCGTGGCCGAGCAGCATGCCGTCGATATTGGCTCCG
GTGCCGAGGTTGCCGCCTTGGCCACCACCCTCAACATCGACCTGGTGGTCATCGGCCCCGAGGTGCCGTTGGTGCTGGGT
GTCGCCGACGCCGTGCGGGCCGCGGGCATCGCCTGCTTCGGTCCCTCGGCCGCCGCCGCGCGCATCGAAGGCTCCAAGGC
ATTCGCCAAAGACGTGATGACCGCCGCGGGGGTGCGCACCGCGCGCAGCGAAATCGTGGACAACCCAGCGCATCTGGATG
CGGCCCTGGACCGGTTCGCCAGCGAGCCGGCCTGGGTGGTCAAGGACGACGGACTGGCCGCGGGCAAGGGCGTGGTGGTC
ACCGATGACCGCGCGGCCGCGCGCGCCCACGCCGCGAGCCTGCTCGACGACGGGCATCCCGTGCTGCTGGAGTCCTTCCT
CGACGGGCCCGAGGTCTCCCTCCTGTGCATCGTCGACGGCGAGACGGTGGTGCCGCTGCTGCCGGCCCAGGATCACAAGC
GGGTGGGCGACGGAGACACCGGCCCCAACACCGGAGGTATGGGTGCCTACACCCCGCTGCCGTGGCTGCCTGCTGAGACC
GTCTCCGAGATCGTCGACACCATTGTCAAACCCGTTGCCGCCGAGCTGGTCAAGCGGGACAGTACTTTTACCGGCGTGCT
CTACGCGGGTCTGGCGATCACCTCGAACGGCCCCTCCGTCGTCGAGTTCAACTGCCGCTTCGGTGATCCCGAGACGCAGG
CGGTACTTGCGCTGCTCGAGTCGCCGCTGGGGCAACTGCTCAACGCCGCCGCGATCGGGAATCTCCAGAATTTCGGCCCG
CTGCGCTGGCGGGACGGCAGCGCGGTGACAGTGGTGTTGGCCGCGGAAAACTACCCGTTGCGCCCGCGCACCGGCGACGT
GATCACCGGCGCTGAGGCAGAGGGTGTGCTGCATGCCGGTACGGCCCGACGTGAGGATGGCGCCGTGGTGTCGTCCGGTG
GACGCGTGTTGTCGGTGGTGGGCACCGGTACCGATCTGCAGGCCGCGCGCGCCGATGCTTATGCGAAGATCGAGGCGATT
CGCCTGCCGGGCAGCCACTTCCGCAGCGACATCGGGTTGGCCGCTGCCGAAGGGCGCATCAGCATCTAG

Upstream 100 bases:

>100_bases
CCGGACGTGACGAGTTACTGGCCAAGTTGCTGCGAGGGTACCGCCCGGATGCCTAGCCTGCCCCGTGGCCGGGGTGGCCC
CGACCGCTAGGGTCTGGTGC

Downstream 100 bases:

>100_bases
GGGTATCTAGGGGTACGGTCTAGCCAGCCAGGATCGGTGCGATCCAGCCCAGTGCCTGCGGCACTTGGGACAGCCAATAT
CCGCCCTCGTGCGCGCCCGG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MESLRVLIIGSGGREHALAIALKRDPRVDYLAIAPGNAGTAAVAEQHAVDIGSGAEVAALATTLNIDLVVIGPEVPLVLG
VADAVRAAGIACFGPSAAAARIEGSKAFAKDVMTAAGVRTARSEIVDNPAHLDAALDRFASEPAWVVKDDGLAAGKGVVV
TDDRAAARAHAASLLDDGHPVLLESFLDGPEVSLLCIVDGETVVPLLPAQDHKRVGDGDTGPNTGGMGAYTPLPWLPAET
VSEIVDTIVKPVAAELVKRDSTFTGVLYAGLAITSNGPSVVEFNCRFGDPETQAVLALLESPLGQLLNAAAIGNLQNFGP
LRWRDGSAVTVVLAAENYPLRPRTGDVITGAEAEGVLHAGTARREDGAVVSSGGRVLSVVGTGTDLQAARADAYAKIEAI
RLPGSHFRSDIGLAAAEGRISI

Sequences:

>Translated_422_residues
MESLRVLIIGSGGREHALAIALKRDPRVDYLAIAPGNAGTAAVAEQHAVDIGSGAEVAALATTLNIDLVVIGPEVPLVLG
VADAVRAAGIACFGPSAAAARIEGSKAFAKDVMTAAGVRTARSEIVDNPAHLDAALDRFASEPAWVVKDDGLAAGKGVVV
TDDRAAARAHAASLLDDGHPVLLESFLDGPEVSLLCIVDGETVVPLLPAQDHKRVGDGDTGPNTGGMGAYTPLPWLPAET
VSEIVDTIVKPVAAELVKRDSTFTGVLYAGLAITSNGPSVVEFNCRFGDPETQAVLALLESPLGQLLNAAAIGNLQNFGP
LRWRDGSAVTVVLAAENYPLRPRTGDVITGAEAEGVLHAGTARREDGAVVSSGGRVLSVVGTGTDLQAARADAYAKIEAI
RLPGSHFRSDIGLAAAEGRISI
>Mature_422_residues
MESLRVLIIGSGGREHALAIALKRDPRVDYLAIAPGNAGTAAVAEQHAVDIGSGAEVAALATTLNIDLVVIGPEVPLVLG
VADAVRAAGIACFGPSAAAARIEGSKAFAKDVMTAAGVRTARSEIVDNPAHLDAALDRFASEPAWVVKDDGLAAGKGVVV
TDDRAAARAHAASLLDDGHPVLLESFLDGPEVSLLCIVDGETVVPLLPAQDHKRVGDGDTGPNTGGMGAYTPLPWLPAET
VSEIVDTIVKPVAAELVKRDSTFTGVLYAGLAITSNGPSVVEFNCRFGDPETQAVLALLESPLGQLLNAAAIGNLQNFGP
LRWRDGSAVTVVLAAENYPLRPRTGDVITGAEAEGVLHAGTARREDGAVVSSGGRVLSVVGTGTDLQAARADAYAKIEAI
RLPGSHFRSDIGLAAAEGRISI

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=42.688679245283, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=42.688679245283, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=42.688679245283, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=42.688679245283, Blast_Score=320, Evalue=2e-87,
Organism=Escherichia coli, GI1790438, Length=430, Percent_Identity=44.1860465116279, Blast_Score=310, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=38.3886255924171, Blast_Score=244, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=38.2022471910112, Blast_Score=260, Evalue=3e-70,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=41.8981481481481, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=41.8981481481481, Blast_Score=281, Evalue=9e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 43200; Mature: 43200

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLRVLIIGSGGREHALAIALKRDPRVDYLAIAPGNAGTAAVAEQHAVDIGSGAEVAAL
CCCEEEEEEECCCCCEEEEEEEECCCCCCEEEECCCCCCCHHHHHHHCEECCCCCCEEEE
ATTLNIDLVVIGPEVPLVLGVADAVRAAGIACFGPSAAAARIEGSKAFAKDVMTAAGVRT
EEEEEEEEEEECCCCCEEEEHHHHHHHCCEEEECCCCCCEEECCCHHHHHHHHHHHHHHH
ARSEIVDNPAHLDAALDRFASEPAWVVKDDGLAAGKGVVVTDDRAAARAHAASLLDDGHP
HHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCH
VLLESFLDGPEVSLLCIVDGETVVPLLPAQDHKRVGDGDTGPNTGGMGAYTPLPWLPAET
HHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
VSEIVDTIVKPVAAELVKRDSTFTGVLYAGLAITSNGPSVVEFNCRFGDPETQAVLALLE
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHH
SPLGQLLNAAAIGNLQNFGPLRWRDGSAVTVVLAAENYPLRPRTGDVITGAEAEGVLHAG
HHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEEECCCCCCCCCCCEEECCCCCCEEECC
TARREDGAVVSSGGRVLSVVGTGTDLQAARADAYAKIEAIRLPGSHFRSDIGLAAAEGRI
CCCCCCCCEEECCCEEEEEEECCCCCHHHHCCCEEEEEEEECCCHHHHHCCCEEECCCEE
SI
CC
>Mature Secondary Structure
MESLRVLIIGSGGREHALAIALKRDPRVDYLAIAPGNAGTAAVAEQHAVDIGSGAEVAAL
CCCEEEEEEECCCCCEEEEEEEECCCCCCEEEECCCCCCCHHHHHHHCEECCCCCCEEEE
ATTLNIDLVVIGPEVPLVLGVADAVRAAGIACFGPSAAAARIEGSKAFAKDVMTAAGVRT
EEEEEEEEEEECCCCCEEEEHHHHHHHCCEEEECCCCCCEEECCCHHHHHHHHHHHHHHH
ARSEIVDNPAHLDAALDRFASEPAWVVKDDGLAAGKGVVVTDDRAAARAHAASLLDDGHP
HHHHHCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHHHCCCCH
VLLESFLDGPEVSLLCIVDGETVVPLLPAQDHKRVGDGDTGPNTGGMGAYTPLPWLPAET
HHHHHHCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
VSEIVDTIVKPVAAELVKRDSTFTGVLYAGLAITSNGPSVVEFNCRFGDPETQAVLALLE
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHH
SPLGQLLNAAAIGNLQNFGPLRWRDGSAVTVVLAAENYPLRPRTGDVITGAEAEGVLHAG
HHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEEECCCCCCCCCCCEEECCCCCCEEECC
TARREDGAVVSSGGRVLSVVGTGTDLQAARADAYAKIEAIRLPGSHFRSDIGLAAAEGRI
CCCCCCCCEEECCCEEEEEEECCCCCHHHHCCCEEEEEEEECCCHHHHHCCCEEECCCEE
SI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]