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The map label for this gene is mhpD3 [H]

Identifier: 169627727

GI number: 169627727

Start: 623644

End: 624429

Strand: Direct

Name: mhpD3 [H]

Synonym: MAB_0624

Alternate gene names: 169627727

Gene position: 623644-624429 (Clockwise)

Preceding gene: 169627726

Following gene: 169627728

Centisome position: 12.31

GC content: 63.23

Gene sequence:

>786_bases
ATGCTTAGTTCCGAGGTACGCGATTCGATCGCGGCCGACCTGGCCGAGGCCGAGCGCACCCGAGTGGCGATCGCGCCGCC
CACCGACACGCATCCGGATTTTGACGTGGTCGACGCCTATGAGATCCAGCTCATGAATATTCGCTCCCGTCTAGCGGACG
GCGCCAAGGTCGTGGGGCACAAGGTGGGCCTATCGTCCGAGGCGATGCAGAAGATGATGGGCGTCCACGAGCCGGACTAT
GGGCATCTGTTGGCCGATATGGAGCTGTTCGAGGATGTGGCGGCCTCGGCGTCCAAGTTCCTGTTCCCGCGGGTTGAGGT
GGAGGTCGGGTTCATCCTGGCCGCAGACCTGCCCGGCGAAGATTGCACCGAGGACGATGTGCTGGCCGCGACCGCGGCGT
ACGTGCCGTCCATCGAGGTGGTGGACAGCCGGATCACCAACTGGCAGATCAAGATCTGCGACACCATCGCTGACAACGCC
TCCTCTGCGGGGTTTGTCGTTGGTAAGCAACGGGTTTCACCCAAGGACATCGACATCAAGTCGATCGACGCGGTGCTGAC
CTGCAACGGTGAGAAGTTGGCCGAGGGGAGAAGCGATGCGGTACTGGGTAATCCGGTGACATCGGTGGCATGGCTGGCAC
GCAAGGTGGCCTCGTTCGGGGTGCGTCTGCGGGCTGGCGACGTGGTGCTGCCAGGTTCGTGCACGCGGGCGTTCGACGCG
CATCCGGGCGACGAGTTTTTAGCCGAGTTCGCCGGACTTGGTTCGGTCCGACTGGCTTTCGAGTAG

Upstream 100 bases:

>100_bases
GCGGTCGGTATCTGCTCAAATTCGTATGTGAGCGGATTGTCCCTGGCCGACTGCGTCTTCTCCGGACGCCGGGCCGGGAC
CGAGACTGGAAGGCGTATAG

Downstream 100 bases:

>100_bases
AGGAGAGATTGTGGCGTCAAAGGCATCTGTAGCGATCGTCGGTTCGGGAAACATCAGCACCGACCTGCTGTACAAATTGC
AGCGTTCCGAGTGGCTGGAG

Product: hydratase/decarboxylase

Products: NA

Alternate protein names: 2-hydroxypentadienoic acid hydratase 3 [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLSSEVRDSIAADLAEAERTRVAIAPPTDTHPDFDVVDAYEIQLMNIRSRLADGAKVVGHKVGLSSEAMQKMMGVHEPDY
GHLLADMELFEDVAASASKFLFPRVEVEVGFILAADLPGEDCTEDDVLAATAAYVPSIEVVDSRITNWQIKICDTIADNA
SSAGFVVGKQRVSPKDIDIKSIDAVLTCNGEKLAEGRSDAVLGNPVTSVAWLARKVASFGVRLRAGDVVLPGSCTRAFDA
HPGDEFLAEFAGLGSVRLAFE

Sequences:

>Translated_261_residues
MLSSEVRDSIAADLAEAERTRVAIAPPTDTHPDFDVVDAYEIQLMNIRSRLADGAKVVGHKVGLSSEAMQKMMGVHEPDY
GHLLADMELFEDVAASASKFLFPRVEVEVGFILAADLPGEDCTEDDVLAATAAYVPSIEVVDSRITNWQIKICDTIADNA
SSAGFVVGKQRVSPKDIDIKSIDAVLTCNGEKLAEGRSDAVLGNPVTSVAWLARKVASFGVRLRAGDVVLPGSCTRAFDA
HPGDEFLAEFAGLGSVRLAFE
>Mature_261_residues
MLSSEVRDSIAADLAEAERTRVAIAPPTDTHPDFDVVDAYEIQLMNIRSRLADGAKVVGHKVGLSSEAMQKMMGVHEPDY
GHLLADMELFEDVAASASKFLFPRVEVEVGFILAADLPGEDCTEDDVLAATAAYVPSIEVVDSRITNWQIKICDTIADNA
SSAGFVVGKQRVSPKDIDIKSIDAVLTCNGEKLAEGRSDAVLGNPVTSVAWLARKVASFGVRLRAGDVVLPGSCTRAFDA
HPGDEFLAEFAGLGSVRLAFE

Specific function: Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family. MhpD subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=259, Percent_Identity=38.6100386100386, Blast_Score=187, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =4.2.1.80 [H]

Molecular weight: Translated: 27894; Mature: 27894

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSSEVRDSIAADLAEAERTRVAIAPPTDTHPDFDVVDAYEIQLMNIRSRLADGAKVVGH
CCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHEEHHHHHHHHCCCHHHHHH
KVGLSSEAMQKMMGVHEPDYGHLLADMELFEDVAASASKFLFPRVEVEVGFILAADLPGE
HCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCC
DCTEDDVLAATAAYVPSIEVVDSRITNWQIKICDTIADNASSAGFVVGKQRVSPKDIDIK
CCCCCHHHHHHHHHCCCHHHHHCCCCEEEEEEEEEHHCCCCCCCEEEECCCCCCCCCCHH
SIDAVLTCNGEKLAEGRSDAVLGNPVTSVAWLARKVASFGVRLRAGDVVLPGSCTRAFDA
HCCEEEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCEEECCCCCCCCCC
HPGDEFLAEFAGLGSVRLAFE
CCCHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MLSSEVRDSIAADLAEAERTRVAIAPPTDTHPDFDVVDAYEIQLMNIRSRLADGAKVVGH
CCCHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHEEHHHHHHHHCCCHHHHHH
KVGLSSEAMQKMMGVHEPDYGHLLADMELFEDVAASASKFLFPRVEVEVGFILAADLPGE
HCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCCCC
DCTEDDVLAATAAYVPSIEVVDSRITNWQIKICDTIADNASSAGFVVGKQRVSPKDIDIK
CCCCCHHHHHHHHHCCCHHHHHCCCCEEEEEEEEEHHCCCCCCCEEEECCCCCCCCCCHH
SIDAVLTCNGEKLAEGRSDAVLGNPVTSVAWLARKVASFGVRLRAGDVVLPGSCTRAFDA
HCCEEEEECCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCEEECCCCCCCCCC
HPGDEFLAEFAGLGSVRLAFE
CCCHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791097; 12823813 [H]