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The map label for this gene is caiD [H]

Identifier: 169627709

GI number: 169627709

Start: 605547

End: 606305

Strand: Reverse

Name: caiD [H]

Synonym: MAB_0606c

Alternate gene names: 169627709

Gene position: 606305-605547 (Counterclockwise)

Preceding gene: 169627712

Following gene: 169627708

Centisome position: 11.97

GC content: 64.03

Gene sequence:

>759_bases
ATGGGCATCACCACCGCGACGGTTGAACCGGGCATCGTCACCGTCACCGTCGACTATCCGAAGGTCAACGCCATCCCGTC
GCGCGGATGGTTTGAGCTGGCCGACGCCATCCTGGCCGCGGGCAACGACCCGTCCACGCACGTGGTGATCCTGCGGGCCG
AGGGCCGCGGGTTCAACGCCGGTGCCGACATCAAGGAGATGCAGGCGACCGACGGCTTCACCGCACTGATCGACACCAAT
CGGGGCTGCTTCGAGGCCTTCAGTGCGGTGTATCGCTGCAAGGTGCCCGTCATCGCCGCCGTCAACGGCTTCTGCCTCGG
CGGCGGAATTGGCTTGGTGGGCAACGCCGATGTGATCGTCGCCGCCGATGACGCCGTGTTCAGCGTGCCGGAAGTCGATC
GTGGCGCGCTGGGCACTGCCACCCATCTGCAACGCCTTGTCCCCCAGCATGTGATGCGCCGAATGTTCTACACGGCAGCC
AAGATCGACGCAAAGACGTTGCATCACTACGGGTCCATCCACGAGGTCGTGCCACGCGCGGAACTCGACGAGGCAGCACT
ACGCGTTGCGCGTGATATCGCCGCCAAGGACACCCGGGTGATCCGGGCCGCCAAGGAGGCCATCAACTTCATCGATCCGC
AGGACGTCGAGTCGAGTTACCGCATGGAGCAGGGCTTCACATTCGAACTCAACCTTGCCGGAGTCGCCGACGAACACCGT
GATGCGTTCGTCGAGACCGGAAAGCCGAAGGGGAACTAA

Upstream 100 bases:

>100_bases
TCGCACGTGACTCCTTGCCCTGTTCCTGCTGTTTGTCCTGCGCTTTCGAGCCATGGTAGCGTACCAAGCAAGTGCTTGTT
TCGGTACCTTGAGGAGTCTC

Downstream 100 bases:

>100_bases
CTCATGGGAGACAAGCGCACCACCCTGGACGAGGTTGTCGCCGAGCTGCGCAGTGGGATGACCATCGGCCTGGGCGGCTG
GGGTTCCCGCCGCAAGCCCA

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MGITTATVEPGIVTVTVDYPKVNAIPSRGWFELADAILAAGNDPSTHVVILRAEGRGFNAGADIKEMQATDGFTALIDTN
RGCFEAFSAVYRCKVPVIAAVNGFCLGGGIGLVGNADVIVAADDAVFSVPEVDRGALGTATHLQRLVPQHVMRRMFYTAA
KIDAKTLHHYGSIHEVVPRAELDEAALRVARDIAAKDTRVIRAAKEAINFIDPQDVESSYRMEQGFTFELNLAGVADEHR
DAFVETGKPKGN

Sequences:

>Translated_252_residues
MGITTATVEPGIVTVTVDYPKVNAIPSRGWFELADAILAAGNDPSTHVVILRAEGRGFNAGADIKEMQATDGFTALIDTN
RGCFEAFSAVYRCKVPVIAAVNGFCLGGGIGLVGNADVIVAADDAVFSVPEVDRGALGTATHLQRLVPQHVMRRMFYTAA
KIDAKTLHHYGSIHEVVPRAELDEAALRVARDIAAKDTRVIRAAKEAINFIDPQDVESSYRMEQGFTFELNLAGVADEHR
DAFVETGKPKGN
>Mature_251_residues
GITTATVEPGIVTVTVDYPKVNAIPSRGWFELADAILAAGNDPSTHVVILRAEGRGFNAGADIKEMQATDGFTALIDTNR
GCFEAFSAVYRCKVPVIAAVNGFCLGGGIGLVGNADVIVAADDAVFSVPEVDRGALGTATHLQRLVPQHVMRRMFYTAAK
IDAKTLHHYGSIHEVVPRAELDEAALRVARDIAAKDTRVIRAAKEAINFIDPQDVESSYRMEQGFTFELNLAGVADEHRD
AFVETGKPKGN

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=30.8, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI194097323, Length=241, Percent_Identity=29.045643153527, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI70995211, Length=231, Percent_Identity=29.4372294372294, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI221142681, Length=214, Percent_Identity=33.1775700934579, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=30.2040816326531, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=28.0487804878049, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87082183, Length=237, Percent_Identity=26.5822784810127, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17534483, Length=246, Percent_Identity=30.0813008130081, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=28.4552845528455, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=252, Percent_Identity=30.952380952381, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=264, Percent_Identity=27.2727272727273, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=246, Percent_Identity=28.8617886178862, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=185, Percent_Identity=29.7297297297297, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=214, Percent_Identity=24.2990654205607, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=221, Percent_Identity=30.316742081448, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653477, Length=221, Percent_Identity=30.316742081448, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19920382, Length=225, Percent_Identity=28, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922422, Length=199, Percent_Identity=25.1256281407035, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24653139, Length=256, Percent_Identity=29.296875, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 27025; Mature: 26894

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGITTATVEPGIVTVTVDYPKVNAIPSRGWFELADAILAAGNDPSTHVVILRAEGRGFNA
CCCCCEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCC
GADIKEMQATDGFTALIDTNRGCFEAFSAVYRCKVPVIAAVNGFCLGGGIGLVGNADVIV
CCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCEEEECCEEEECCCCEEE
AADDAVFSVPEVDRGALGTATHLQRLVPQHVMRRMFYTAAKIDAKTLHHYGSIHEVVPRA
EECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
ELDEAALRVARDIAAKDTRVIRAAKEAINFIDPQDVESSYRMEQGFTFELNLAGVADEHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHH
DAFVETGKPKGN
HHHHCCCCCCCC
>Mature Secondary Structure 
GITTATVEPGIVTVTVDYPKVNAIPSRGWFELADAILAAGNDPSTHVVILRAEGRGFNA
CCCCEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCC
GADIKEMQATDGFTALIDTNRGCFEAFSAVYRCKVPVIAAVNGFCLGGGIGLVGNADVIV
CCCHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCEEEECCEEEECCCCEEE
AADDAVFSVPEVDRGALGTATHLQRLVPQHVMRRMFYTAAKIDAKTLHHYGSIHEVVPRA
EECCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
ELDEAALRVARDIAAKDTRVIRAAKEAINFIDPQDVESSYRMEQGFTFELNLAGVADEHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEEEECCCCCCHH
DAFVETGKPKGN
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA