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The map label for this gene is fadE25 [H]

Identifier: 169627700

GI number: 169627700

Start: 598226

End: 599419

Strand: Direct

Name: fadE25 [H]

Synonym: MAB_0597

Alternate gene names: 169627700

Gene position: 598226-599419 (Clockwise)

Preceding gene: 169627694

Following gene: 169627701

Centisome position: 11.81

GC content: 63.74

Gene sequence:

>1194_bases
GTGGTTGAGTCGCAAGTATCACCGGCGCCAGCCGGAGAACCTATGTCGGAGATGTCCGATGATCAGGTACGCACCGAAAT
CCGGGAATGGCTGGCGGAGAACCTCGTCGGAGAGTTCGCCGAGCTGAAGGGGCTCGGCGGGCCCGGCAGCGAGCACGAGG
CCTTCGAGGAGCGTCTTGCCTGGAATCGTCACCTGGCCGCGGCGGGCTGGACCTGCCTGGGCTGGCCGGTAGAGCACGGG
GGCCGCGGGGCCACGGTGAGCCAACGCGTCATCTTCTACGAGGAGTACGCCCGTGCCGAGGCGCCGCACAAGGTCAACCA
CCTGGGTGAGGAGCTGCTCGGTCCGACGCTTATCGCCTACGGCACTCCCGAGCAACAGCAGCGCTTCCTGCCCGCCATCC
GCGACGTCACGGAGCTGTGGTGCCAGGGATATTCCGAACCCGGCGCGGGCTCGGATCTGGCCAACGTGTCCACCTCTGCA
GTGCTGGACGGGGGCGACTGGGTGATCAACGGGCAGAAGGTGTGGACCTCACTGGCACTGCAGTCGCAATGGTGCTTCGT
GGTGGCGCGCACCGAGCCCGGCTCCTCGCGTCACAAGGGTTTGTCGTATCTGCTTGTGCCCCTTGACCAGCCGGGTGTCG
AGATTCGGCCCATCATCCAGTTGACCGGCACCTCGGAGTTCAACGAGGTGTTCTTCGACAACGCGCGTACCTCCGCCGAT
TTGGTGGTCGGCGAGCCCGGCGACGGCTGGCGCGTCGCCATGGGTACGCTGACTTTTGAGCGTGGTGTCTCGACCCTTGG
TAACCAGATCACCTATGCCCGTGAGCTTTCCAGGCTCGCCGAGCTTGCCAAGTCCAACGGCGCGGCAGACGATCCGGCCA
TTCGTGAACGCTTGGCGCAGGCCTACGTGGGTATGCGTACCATGCGCGCCTATGCGCTGGCCACCATGGACGAAGAACGG
CCCGGCCAGGACAATGTGTCAAAGCTGTTGTGGGCCAACTGGCATCGTGACCTAGGTGAATTGGCGATGGAAATCATCGG
CACGTCGACGCTGCTGACGAATGCGGGCGAGATGGACGAATGGCAGCGGTTGTTCCTGTTTTCTCGTGCTGACACCATCT
ACGGCGGATCCAATGAGATCCAGCGCAACATCATCTCCGAGCGGGTGCTCGGTCTACCCAGAGAGGCTCGCTAA

Upstream 100 bases:

>100_bases
CTGCCGGAATGGTGAGCCCGGGTGTCTGCTGCGCCGATTCCATACGCGTCCGACCCACCTTCCAATAACAAGCAAGTGCT
TGGTAGGTTAGCCTACGGGA

Downstream 100 bases:

>100_bases
ATGAACCTGGCAGAGGCGCCGACAGAGATCGAGGGCCATGGCCTCCTGAAGGGCAAGGCCGTCGTCGTGACCGCAGCGGC
CGGCACCGGAATCGGCTCGT

Product: acyl-CoA dehydrogenase FadE

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MVESQVSPAPAGEPMSEMSDDQVRTEIREWLAENLVGEFAELKGLGGPGSEHEAFEERLAWNRHLAAAGWTCLGWPVEHG
GRGATVSQRVIFYEEYARAEAPHKVNHLGEELLGPTLIAYGTPEQQQRFLPAIRDVTELWCQGYSEPGAGSDLANVSTSA
VLDGGDWVINGQKVWTSLALQSQWCFVVARTEPGSSRHKGLSYLLVPLDQPGVEIRPIIQLTGTSEFNEVFFDNARTSAD
LVVGEPGDGWRVAMGTLTFERGVSTLGNQITYARELSRLAELAKSNGAADDPAIRERLAQAYVGMRTMRAYALATMDEER
PGQDNVSKLLWANWHRDLGELAMEIIGTSTLLTNAGEMDEWQRLFLFSRADTIYGGSNEIQRNIISERVLGLPREAR

Sequences:

>Translated_397_residues
MVESQVSPAPAGEPMSEMSDDQVRTEIREWLAENLVGEFAELKGLGGPGSEHEAFEERLAWNRHLAAAGWTCLGWPVEHG
GRGATVSQRVIFYEEYARAEAPHKVNHLGEELLGPTLIAYGTPEQQQRFLPAIRDVTELWCQGYSEPGAGSDLANVSTSA
VLDGGDWVINGQKVWTSLALQSQWCFVVARTEPGSSRHKGLSYLLVPLDQPGVEIRPIIQLTGTSEFNEVFFDNARTSAD
LVVGEPGDGWRVAMGTLTFERGVSTLGNQITYARELSRLAELAKSNGAADDPAIRERLAQAYVGMRTMRAYALATMDEER
PGQDNVSKLLWANWHRDLGELAMEIIGTSTLLTNAGEMDEWQRLFLFSRADTIYGGSNEIQRNIISERVLGLPREAR
>Mature_397_residues
MVESQVSPAPAGEPMSEMSDDQVRTEIREWLAENLVGEFAELKGLGGPGSEHEAFEERLAWNRHLAAAGWTCLGWPVEHG
GRGATVSQRVIFYEEYARAEAPHKVNHLGEELLGPTLIAYGTPEQQQRFLPAIRDVTELWCQGYSEPGAGSDLANVSTSA
VLDGGDWVINGQKVWTSLALQSQWCFVVARTEPGSSRHKGLSYLLVPLDQPGVEIRPIIQLTGTSEFNEVFFDNARTSAD
LVVGEPGDGWRVAMGTLTFERGVSTLGNQITYARELSRLAELAKSNGAADDPAIRERLAQAYVGMRTMRAYALATMDEER
PGQDNVSKLLWANWHRDLGELAMEIIGTSTLLTNAGEMDEWQRLFLFSRADTIYGGSNEIQRNIISERVLGLPREAR

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557233, Length=346, Percent_Identity=29.4797687861272, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI4501859, Length=336, Percent_Identity=28.2738095238095, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4501857, Length=388, Percent_Identity=25.7731958762887, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI4557231, Length=295, Percent_Identity=25.7627118644068, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI187960098, Length=295, Percent_Identity=25.7627118644068, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI48976061, Length=292, Percent_Identity=26.027397260274, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI211971008, Length=292, Percent_Identity=26.027397260274, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI226958412, Length=266, Percent_Identity=23.6842105263158, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI38505218, Length=154, Percent_Identity=36.3636363636364, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI226958414, Length=215, Percent_Identity=25.5813953488372, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI21361497, Length=403, Percent_Identity=25.3101736972705, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786223, Length=379, Percent_Identity=24.5382585751979, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081958, Length=340, Percent_Identity=24.1176470588235, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17570075, Length=341, Percent_Identity=27.5659824046921, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17508101, Length=353, Percent_Identity=27.7620396600567, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17569725, Length=333, Percent_Identity=26.1261261261261, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17551932, Length=348, Percent_Identity=25.2873563218391, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17534899, Length=333, Percent_Identity=25.2252252252252, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17538396, Length=268, Percent_Identity=25.7462686567164, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71990804, Length=182, Percent_Identity=31.3186813186813, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17505929, Length=293, Percent_Identity=27.9863481228669, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17506239, Length=187, Percent_Identity=31.5508021390374, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI32563615, Length=187, Percent_Identity=31.5508021390374, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356377, Length=346, Percent_Identity=26.3005780346821, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24646207, Length=350, Percent_Identity=26.5714285714286, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24660351, Length=294, Percent_Identity=28.2312925170068, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281363737, Length=346, Percent_Identity=26.5895953757225, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21355753, Length=262, Percent_Identity=26.3358778625954, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24666513, Length=188, Percent_Identity=27.6595744680851, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 43819; Mature: 43819

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVESQVSPAPAGEPMSEMSDDQVRTEIREWLAENLVGEFAELKGLGGPGSEHEAFEERLA
CCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
WNRHLAAAGWTCLGWPVEHGGRGATVSQRVIFYEEYARAEAPHKVNHLGEELLGPTLIAY
HHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEE
GTPEQQQRFLPAIRDVTELWCQGYSEPGAGSDLANVSTSAVLDGGDWVINGQKVWTSLAL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHEEECCCCEEECCHHHHHHHHH
QSQWCFVVARTEPGSSRHKGLSYLLVPLDQPGVEIRPIIQLTGTSEFNEVFFDNARTSAD
CCCEEEEEEECCCCCCHHCCCEEEEEECCCCCCCEEEEEEEECCCHHHHHHHCCCCCCCC
LVVGEPGDGWRVAMGTLTFERGVSTLGNQITYARELSRLAELAKSNGAADDPAIRERLAQ
EEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AYVGMRTMRAYALATMDEERPGQDNVSKLLWANWHRDLGELAMEIIGTSTLLTNAGEMDE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
WQRLFLFSRADTIYGGSNEIQRNIISERVLGLPREAR
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVESQVSPAPAGEPMSEMSDDQVRTEIREWLAENLVGEFAELKGLGGPGSEHEAFEERLA
CCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
WNRHLAAAGWTCLGWPVEHGGRGATVSQRVIFYEEYARAEAPHKVNHLGEELLGPTLIAY
HHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEE
GTPEQQQRFLPAIRDVTELWCQGYSEPGAGSDLANVSTSAVLDGGDWVINGQKVWTSLAL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHEEECCCCEEECCHHHHHHHHH
QSQWCFVVARTEPGSSRHKGLSYLLVPLDQPGVEIRPIIQLTGTSEFNEVFFDNARTSAD
CCCEEEEEEECCCCCCHHCCCEEEEEECCCCCCCEEEEEEEECCCHHHHHHHCCCCCCCC
LVVGEPGDGWRVAMGTLTFERGVSTLGNQITYARELSRLAELAKSNGAADDPAIRERLAQ
EEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AYVGMRTMRAYALATMDEERPGQDNVSKLLWANWHRDLGELAMEIIGTSTLLTNAGEMDE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
WQRLFLFSRADTIYGGSNEIQRNIISERVLGLPREAR
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]