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The map label for this gene is ispF

Identifier: 169627673

GI number: 169627673

Start: 569524

End: 570000

Strand: Direct

Name: ispF

Synonym: MAB_0570

Alternate gene names: 169627673

Gene position: 569524-570000 (Clockwise)

Preceding gene: 169627672

Following gene: 169627674

Centisome position: 11.24

GC content: 66.25

Gene sequence:

>477_bases
ATGAACGCATTGCCCCGGGTGGGCATCGGTACCGATGTGCACCCCATCGAGGAGGGCAAGCCCTGTTGGTTGCTGGGACT
GCTCTTTTCCGATGAGAACGGATGCGAAGGACACTCCGACGGGGACGTTGCCGCGCATGCGCTCTGTGATGCCCTGCTGT
CCGCGGCCGGGCTGGGCGACGTTGGTGCGGTGTTCGGTACCGGACGTCCCGAGTGGACGGGCGTCAGCGGTGCGAAGATG
CTGAGTCACGTGCGGGACCTGCTGCACGACAATGGCTTCCAGATCGGTAATGCCGCGGTGCAAGTCATCGGCAATCGGCC
GAAGGTGGGTCCGCGCCGGGCTGAGGCGCAGGAGCTGCTTTCCGGCCTGATCGGGGCACCGGTTTCGGTATCGGCAACCA
CCACCGACGGGCTGGGTCTGACCGGACGCGGCGAAGGGTTGGCCGCGGTGGCAACGGCGCTCGTCACAGCGCTGTAA

Upstream 100 bases:

>100_bases
GGGTTCACGTGGTGGAAGGCGACCGGTTGGCCTTCAAAATCACCACGACACTCGACATGACATTGGCCGAGGCGATTCTC
GGTGCTACGGAAGGCGTCCA

Downstream 100 bases:

>100_bases
GCTCGCTGGTCGTGACCGGTCACGTTCCGCTTCGGCTGTACGACACCCGTGTGGGTGCCGTGCGTGACTTCGTGCCGCTG
CAGCCCGGGACCGTCTCCAT

Product: putative 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MNALPRVGIGTDVHPIEEGKPCWLLGLLFSDENGCEGHSDGDVAAHALCDALLSAAGLGDVGAVFGTGRPEWTGVSGAKM
LSHVRDLLHDNGFQIGNAAVQVIGNRPKVGPRRAEAQELLSGLIGAPVSVSATTTDGLGLTGRGEGLAAVATALVTAL

Sequences:

>Translated_158_residues
MNALPRVGIGTDVHPIEEGKPCWLLGLLFSDENGCEGHSDGDVAAHALCDALLSAAGLGDVGAVFGTGRPEWTGVSGAKM
LSHVRDLLHDNGFQIGNAAVQVIGNRPKVGPRRAEAQELLSGLIGAPVSVSATTTDGLGLTGRGEGLAAVATALVTAL
>Mature_158_residues
MNALPRVGIGTDVHPIEEGKPCWLLGLLFSDENGCEGHSDGDVAAHALCDALLSAAGLGDVGAVFGTGRPEWTGVSGAKM
LSHVRDLLHDNGFQIGNAAVQVIGNRPKVGPRRAEAQELLSGLIGAPVSVSATTTDGLGLTGRGEGLAAVATALVTAL

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=153, Percent_Identity=39.8692810457516, Blast_Score=99, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_MYCA9 (B1MGY4)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001701322.1
- ProteinModelPortal:   B1MGY4
- SMR:   B1MGY4
- EnsemblBacteria:   EBMYCT00000005415
- GeneID:   5963106
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_0570
- GeneTree:   EBGT00050000016933
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- BioCyc:   MABS561007:MAB_0570-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 15942; Mature: 15942

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALPRVGIGTDVHPIEEGKPCWLLGLLFSDENGCEGHSDGDVAAHALCDALLSAAGLGD
CCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
VGAVFGTGRPEWTGVSGAKMLSHVRDLLHDNGFQIGNAAVQVIGNRPKVGPRRAEAQELL
CHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCHHHEEECCCCCCCCCHHHHHHHH
SGLIGAPVSVSATTTDGLGLTGRGEGLAAVATALVTAL
HHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNALPRVGIGTDVHPIEEGKPCWLLGLLFSDENGCEGHSDGDVAAHALCDALLSAAGLGD
CCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
VGAVFGTGRPEWTGVSGAKMLSHVRDLLHDNGFQIGNAAVQVIGNRPKVGPRRAEAQELL
CHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCHHHEEECCCCCCCCCHHHHHHHH
SGLIGAPVSVSATTTDGLGLTGRGEGLAAVATALVTAL
HHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA