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The map label for this gene is dacB [H]

Identifier: 169627623

GI number: 169627623

Start: 519729

End: 521126

Strand: Direct

Name: dacB [H]

Synonym: MAB_0519

Alternate gene names: 169627623

Gene position: 519729-521126 (Clockwise)

Preceding gene: 169627621

Following gene: 169627624

Centisome position: 10.26

GC content: 68.1

Gene sequence:

>1398_bases
ATGGTCACCGCCAGGGCACTTGATCCGCGTCATTGGCGGCGCTCTACCCACGCTCTGCTTGCCGTCGCCGTCGTATTGGC
GATTGCCGCGCTGGTGCTGCTGGCGTCCCTGACCACGGGGGCCTCCACCGCGCATCAGTTGCAGAGCGCCGATCCGGAGC
CGGCGCTGATTACCCCGGCGCCAGGTGTGGTGCCGGTTTCCGATTCGGCACCGATTCCGACGGCGGATGGTTTGGCTCAG
GCGCTGGACAGGGCGTTGTCCGACCCGGCACTGGGCATGTTCACCGGCCGGGTTACCGACGCGCTGACCGGCCGTGAGTT
GTGGCAGCAGGGCTCCACGGTGCCGATGGTGCCGGCCTCGACCAACAAGGTGTTGACCGCCGCGGCGGCCCTGCTCACTC
TCGAACGCGACGCCAAGCTGACCACTTCGGTGGTGGCCGATACTGCCGGGCAACGCGGACTGGTGACACTGGTTGGTGGC
GGCGATCCGATCGTCAGCGCGGCACCGGCAGGCACCGACACCTGGTACCGCGATGCCGCGCGAATCAGCGACCTCGCCGA
CCAGGTGCGCAAGTCCGGTGTCGCCGTCACCTCAATCACCGTTGATATCAGCCGATTCGGTGGGCCCTCGATGGCGCCCG
GGTGGGACCCGGCCGATATCGAAGGCGGCGATGTCGCGCCCATCCAGGCGGTGATGCTCGATGCCGGACGTACGCAGCCG
ACCGACTATGACTCGCGCCGTTCCACCACTCCCGCCCTGGACGCGGGCCGGGCGCTCGCCGCCGCGCTCGGTGTGGACCC
GGAGGCGGTGCGCCTTGACGCGGCCCCTCCGACGGCGAAATCGATTGCCTCCGTGCAATCGGCGCCGCTGATGGAGCGGC
TGCGGGAGATGATGAACGCCTCCGACAACGTCATGGCGGAAACCATCGGCCGGGAGGTGGCCCTGGCCACCGGGCGTGCG
CAGACCTTCTCCGGCACGGTGGACGCCGTGACAAGTCAATTGCGCAGTGCGGGGATCGATCTCACGGACCTGACATTGCG
TGATTCCAGCGGACTGTCGGTGGACGACCGGGTTACCGCGCGCACGTTGGACGAGGTGATCGGAGCCGCCGCCGGCCCCG
ACAAGCCGAAGCTGCGACCACTTCTCGATGTGCTGCCCATCGCCGGTGGCAGCGGCACCCTGTCCGAGCGCTTTGTCACT
CAGAACCAAACCTCGGCGGGGTGGTTGCGGGCCAAGACGGGATCGCTCACCGGTGTGAACACGCTGGCCGGGGTGGTCAC
TGACGTCAGCGGGCGGGTACTGACCTTCTCTCTGATGCAGAATCACGCCACCGCGCCCACCGCACGTAACGCGGTGGACA
ACACGGCTGCCGTGCTGCGGTCCTGCGGATGTAGCTGA

Upstream 100 bases:

>100_bases
CGAACTCCACACCCGCTCCTTGAATGTCGGTGGTACTTGGCAGTCGTCTTCGCGCAAGCGCTCATCCAGCGCGCACCAGG
ATAGCGATACCCTGCAGAGC

Downstream 100 bases:

>100_bases
TGGCTGAGTCGGGACGGGTCGTGCAGAGCGGCGGCGTTGGAACCAAGAGTCCCGAGTTGACGGTTGGCCGCATGGTCAAC
TGGGAATTCGCCGCGACCGT

Product: peptidase S13 (D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MVTARALDPRHWRRSTHALLAVAVVLAIAALVLLASLTTGASTAHQLQSADPEPALITPAPGVVPVSDSAPIPTADGLAQ
ALDRALSDPALGMFTGRVTDALTGRELWQQGSTVPMVPASTNKVLTAAAALLTLERDAKLTTSVVADTAGQRGLVTLVGG
GDPIVSAAPAGTDTWYRDAARISDLADQVRKSGVAVTSITVDISRFGGPSMAPGWDPADIEGGDVAPIQAVMLDAGRTQP
TDYDSRRSTTPALDAGRALAAALGVDPEAVRLDAAPPTAKSIASVQSAPLMERLREMMNASDNVMAETIGREVALATGRA
QTFSGTVDAVTSQLRSAGIDLTDLTLRDSSGLSVDDRVTARTLDEVIGAAAGPDKPKLRPLLDVLPIAGGSGTLSERFVT
QNQTSAGWLRAKTGSLTGVNTLAGVVTDVSGRVLTFSLMQNHATAPTARNAVDNTAAVLRSCGCS

Sequences:

>Translated_465_residues
MVTARALDPRHWRRSTHALLAVAVVLAIAALVLLASLTTGASTAHQLQSADPEPALITPAPGVVPVSDSAPIPTADGLAQ
ALDRALSDPALGMFTGRVTDALTGRELWQQGSTVPMVPASTNKVLTAAAALLTLERDAKLTTSVVADTAGQRGLVTLVGG
GDPIVSAAPAGTDTWYRDAARISDLADQVRKSGVAVTSITVDISRFGGPSMAPGWDPADIEGGDVAPIQAVMLDAGRTQP
TDYDSRRSTTPALDAGRALAAALGVDPEAVRLDAAPPTAKSIASVQSAPLMERLREMMNASDNVMAETIGREVALATGRA
QTFSGTVDAVTSQLRSAGIDLTDLTLRDSSGLSVDDRVTARTLDEVIGAAAGPDKPKLRPLLDVLPIAGGSGTLSERFVT
QNQTSAGWLRAKTGSLTGVNTLAGVVTDVSGRVLTFSLMQNHATAPTARNAVDNTAAVLRSCGCS
>Mature_465_residues
MVTARALDPRHWRRSTHALLAVAVVLAIAALVLLASLTTGASTAHQLQSADPEPALITPAPGVVPVSDSAPIPTADGLAQ
ALDRALSDPALGMFTGRVTDALTGRELWQQGSTVPMVPASTNKVLTAAAALLTLERDAKLTTSVVADTAGQRGLVTLVGG
GDPIVSAAPAGTDTWYRDAARISDLADQVRKSGVAVTSITVDISRFGGPSMAPGWDPADIEGGDVAPIQAVMLDAGRTQP
TDYDSRRSTTPALDAGRALAAALGVDPEAVRLDAAPPTAKSIASVQSAPLMERLREMMNASDNVMAETIGREVALATGRA
QTFSGTVDAVTSQLRSAGIDLTDLTLRDSSGLSVDDRVTARTLDEVIGAAAGPDKPKLRPLLDVLPIAGGSGTLSERFVT
QNQTSAGWLRAKTGSLTGVNTLAGVVTDVSGRVLTFSLMQNHATAPTARNAVDNTAAVLRSCGCS

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=168, Percent_Identity=35.1190476190476, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47698; Mature: 47698

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTARALDPRHWRRSTHALLAVAVVLAIAALVLLASLTTGASTAHQLQSADPEPALITPA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEECCC
PGVVPVSDSAPIPTADGLAQALDRALSDPALGMFTGRVTDALTGRELWQQGSTVPMVPAS
CCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCC
TNKVLTAAAALLTLERDAKLTTSVVADTAGQRGLVTLVGGGDPIVSAAPAGTDTWYRDAA
CCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCCHHHHHHH
RISDLADQVRKSGVAVTSITVDISRFGGPSMAPGWDPADIEGGDVAPIQAVMLDAGRTQP
HHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCC
TDYDSRRSTTPALDAGRALAAALGVDPEAVRLDAAPPTAKSIASVQSAPLMERLREMMNA
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHEEECCCCCCHHHHHHHHCCHHHHHHHHHHCC
SDNVMAETIGREVALATGRAQTFSGTVDAVTSQLRSAGIDLTDLTLRDSSGLSVDDRVTA
CCHHHHHHHCCHHEEECCCCHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHH
RTLDEVIGAAAGPDKPKLRPLLDVLPIAGGSGTLSERFVTQNQTSAGWLRAKTGSLTGVN
HHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCHH
TLAGVVTDVSGRVLTFSLMQNHATAPTARNAVDNTAAVLRSCGCS
HHHHHHHCCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVTARALDPRHWRRSTHALLAVAVVLAIAALVLLASLTTGASTAHQLQSADPEPALITPA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEECCC
PGVVPVSDSAPIPTADGLAQALDRALSDPALGMFTGRVTDALTGRELWQQGSTVPMVPAS
CCEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCC
TNKVLTAAAALLTLERDAKLTTSVVADTAGQRGLVTLVGGGDPIVSAAPAGTDTWYRDAA
CCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEECCCCCEECCCCCCCHHHHHHH
RISDLADQVRKSGVAVTSITVDISRFGGPSMAPGWDPADIEGGDVAPIQAVMLDAGRTQP
HHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCC
TDYDSRRSTTPALDAGRALAAALGVDPEAVRLDAAPPTAKSIASVQSAPLMERLREMMNA
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHEEECCCCCCHHHHHHHHCCHHHHHHHHHHCC
SDNVMAETIGREVALATGRAQTFSGTVDAVTSQLRSAGIDLTDLTLRDSSGLSVDDRVTA
CCHHHHHHHCCHHEEECCCCHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHH
RTLDEVIGAAAGPDKPKLRPLLDVLPIAGGSGTLSERFVTQNQTSAGWLRAKTGSLTGVN
HHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCHH
TLAGVVTDVSGRVLTFSLMQNHATAPTARNAVDNTAAVLRSCGCS
HHHHHHHCCCCCEEEEEHHHCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]