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The map label for this gene is hapE [H]

Identifier: 169627617

GI number: 169627617

Start: 514762

End: 516351

Strand: Direct

Name: hapE [H]

Synonym: MAB_0513

Alternate gene names: 169627617

Gene position: 514762-516351 (Clockwise)

Preceding gene: 169627616

Following gene: 169627618

Centisome position: 10.16

GC content: 58.81

Gene sequence:

>1590_bases
ATGACTAGTGCGCGTGTGCGGGACGGCGAAGCCCGCGGGCTTCGCCAGGGTGTTCCCGACCATGAGGTCGTGATCATCGG
CGCCGGCTTCGGGGGTATCGGTGCCGGAGTCGAATTGACCAAAAGGGGGGTCCACGACTTTGTGATCTTGGAGAAATGGG
GGGCTGCCGGCGGCACCTGGCACGCCAACAAATACCCAGGCGTCGCAGTCGACATACCGACTTTCATCTACAGCTTCTCC
TACGACCAGAAGACGACCTGGTCGAAGTTCTTCACACCCGGAGACGAGCTAGAGCAGTACGCCAACGAACTCGTCGACAA
GCATGAGCTGCGCGGCAAGATTCGATTCAACACACGGGTGGTGCGCCAGGATTTCGATGAGTCCCAGAATATCTGGGTGC
TCACACTGGAGGGCGGCGGCGTGGTCACCGGCCGGCACGTCATCGCAGCCACCGGAGGGCTGGAGCAACCCAAGCTCCCG
GAGATAGACGGTATCGACTCCTACAAGGGCGTGCTTATGCATACAGCGCTGTGGGACAAGGATATTGAGCTCGCTGGAAA
GCGAGTAGCCGTCATCGGGACCGGTGCCACGTCGCTACAGATGGTTCCGGAGATCGCGCCACAGGTGGGTCATCTGACGG
TGTTTCAGCGCACACCTATCTACGTGGGGCCCAAACCGGACTGGCGATACAACGGGTTCTGGCGGGCACTGTTCGGCTTC
CCGGGCGTGGCCAAGGCCATTCGGCACGCCATCAATCTCAGCACCGCATACATTCCGGATGCCGTTTTCAAGCTTGATTC
CAAATATGCCGAATCCGTATCGATGGCGCTGGCAAACAGGATTCGGGCGTGGATGCGTACCCAGGTCGACGATCCGGTGA
CTCGGGAAAAGTTGTTGCCCTACTACGGATTCGGATGCAAGCGGCCCTCGTTCTCCAATACCTATCTCAAAACCTTCAAC
CGTCCCAATGTGGCACTGGTGACCGAACCGATCCAGCGGATCACCGAGAAGGGACTGCTGACAGCAGATGGTGTCGAACA
TGAAGTGGACGTGCTGATCTGTGCCACCGGATTCGCCATCTGGGATCACATGCCCGCCTTCCCCACCACGGGGCGGGGCG
GCAAGGATCTGAAGCAGTTCTTCCAAACCAATCGTTACCAGGCGTACCAGGGCGTTTCGATTCCCGACTACCCCAACTTC
TTCCTCGTTCTGGGGCCGTACGGATACGTTTTCGGGCCGTATCACATGCTCATCGAGTCGACGACGACGCACGCGGCCCG
CGTTATCGCAGAGACCAAGAAACGTGGTGCGACCACCGCCGAAGTCAAGCAGGACGTGCACGACGCGTACTTCCATAAGA
TGCACGAGCGCAACAAAAACCACCTGTGGCTTTCTCCTGCCTGCTCGACCGCCAATACGTATTACATCGACAATCACGGT
GATTCCCCGTTCCGTCCTGGCGGTTTCGGCGAGATGTACTTCCACAACAAGTATTTCCGGCTGGACAACTACCGTTACGG
CACCGAGACGATCGCGCCGGCGGTAGAGTCCGCGCCAAAGCGTAAGCGCAACAAGGAGGTTGTGAGATGA

Upstream 100 bases:

>100_bases
ATTTCCTGGCGGCCTGAATCCGCAATCCACATATCCGTAACTATTAATACGTGGTACTTTCAGTTCGACACATAACGGGT
CGGACTGACAAGGACGTCTA

Downstream 100 bases:

>100_bases
GGCTGCTACCCGCACCCAAACCAGGACTTGTGGTGGTGACCGGCGCCGGCAGCGGCATCGGCCGAGCCATCGCGATCCAA
TTCGCCAAGGGCGGGGCGGA

Product: putative monooxygenase

Products: NA

Alternate protein names: HAPMO; Baeyer-Villiger monooxygenase; BVMO [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MTSARVRDGEARGLRQGVPDHEVVIIGAGFGGIGAGVELTKRGVHDFVILEKWGAAGGTWHANKYPGVAVDIPTFIYSFS
YDQKTTWSKFFTPGDELEQYANELVDKHELRGKIRFNTRVVRQDFDESQNIWVLTLEGGGVVTGRHVIAATGGLEQPKLP
EIDGIDSYKGVLMHTALWDKDIELAGKRVAVIGTGATSLQMVPEIAPQVGHLTVFQRTPIYVGPKPDWRYNGFWRALFGF
PGVAKAIRHAINLSTAYIPDAVFKLDSKYAESVSMALANRIRAWMRTQVDDPVTREKLLPYYGFGCKRPSFSNTYLKTFN
RPNVALVTEPIQRITEKGLLTADGVEHEVDVLICATGFAIWDHMPAFPTTGRGGKDLKQFFQTNRYQAYQGVSIPDYPNF
FLVLGPYGYVFGPYHMLIESTTTHAARVIAETKKRGATTAEVKQDVHDAYFHKMHERNKNHLWLSPACSTANTYYIDNHG
DSPFRPGGFGEMYFHNKYFRLDNYRYGTETIAPAVESAPKRKRNKEVVR

Sequences:

>Translated_529_residues
MTSARVRDGEARGLRQGVPDHEVVIIGAGFGGIGAGVELTKRGVHDFVILEKWGAAGGTWHANKYPGVAVDIPTFIYSFS
YDQKTTWSKFFTPGDELEQYANELVDKHELRGKIRFNTRVVRQDFDESQNIWVLTLEGGGVVTGRHVIAATGGLEQPKLP
EIDGIDSYKGVLMHTALWDKDIELAGKRVAVIGTGATSLQMVPEIAPQVGHLTVFQRTPIYVGPKPDWRYNGFWRALFGF
PGVAKAIRHAINLSTAYIPDAVFKLDSKYAESVSMALANRIRAWMRTQVDDPVTREKLLPYYGFGCKRPSFSNTYLKTFN
RPNVALVTEPIQRITEKGLLTADGVEHEVDVLICATGFAIWDHMPAFPTTGRGGKDLKQFFQTNRYQAYQGVSIPDYPNF
FLVLGPYGYVFGPYHMLIESTTTHAARVIAETKKRGATTAEVKQDVHDAYFHKMHERNKNHLWLSPACSTANTYYIDNHG
DSPFRPGGFGEMYFHNKYFRLDNYRYGTETIAPAVESAPKRKRNKEVVR
>Mature_528_residues
TSARVRDGEARGLRQGVPDHEVVIIGAGFGGIGAGVELTKRGVHDFVILEKWGAAGGTWHANKYPGVAVDIPTFIYSFSY
DQKTTWSKFFTPGDELEQYANELVDKHELRGKIRFNTRVVRQDFDESQNIWVLTLEGGGVVTGRHVIAATGGLEQPKLPE
IDGIDSYKGVLMHTALWDKDIELAGKRVAVIGTGATSLQMVPEIAPQVGHLTVFQRTPIYVGPKPDWRYNGFWRALFGFP
GVAKAIRHAINLSTAYIPDAVFKLDSKYAESVSMALANRIRAWMRTQVDDPVTREKLLPYYGFGCKRPSFSNTYLKTFNR
PNVALVTEPIQRITEKGLLTADGVEHEVDVLICATGFAIWDHMPAFPTTGRGGKDLKQFFQTNRYQAYQGVSIPDYPNFF
LVLGPYGYVFGPYHMLIESTTTHAARVIAETKKRGATTAEVKQDVHDAYFHKMHERNKNHLWLSPACSTANTYYIDNHGD
SPFRPGGFGEMYFHNKYFRLDNYRYGTETIAPAVESAPKRKRNKEVVR

Specific function: Catalyzes a Baeyer-Villiger oxidation reaction, i.e., the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters. Can oxidize a wide range of acetophenone derivatives. Highest activity occurs with co

COG id: COG2072

COG function: function code P; Predicted flavoprotein involved in K+ transport

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-binding monooxygenase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI25145785, Length=348, Percent_Identity=24.7126436781609, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19921694, Length=242, Percent_Identity=24.7933884297521, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR020946 [H]

Pfam domain/function: PF00743 FMO-like [H]

EC number: =1.14.13.84 [H]

Molecular weight: Translated: 59383; Mature: 59252

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSARVRDGEARGLRQGVPDHEVVIIGAGFGGIGAGVELTKRGVHDFVILEKWGAAGGTW
CCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCC
HANKYPGVAVDIPTFIYSFSYDQKTTWSKFFTPGDELEQYANELVDKHELRGKIRFNTRV
CCCCCCCEEEECCCEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCEEEECHHH
VRQDFDESQNIWVLTLEGGGVVTGRHVIAATGGLEQPKLPEIDGIDSYKGVLMHTALWDK
HHHCCCCCCCEEEEEECCCCEEECCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
DIELAGKRVAVIGTGATSLQMVPEIAPQVGHLTVFQRTPIYVGPKPDWRYNGFWRALFGF
CHHHCCCEEEEEECCCHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHCC
PGVAKAIRHAINLSTAYIPDAVFKLDSKYAESVSMALANRIRAWMRTQVDDPVTREKLLP
CHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC
YYGFGCKRPSFSNTYLKTFNRPNVALVTEPIQRITEKGLLTADGVEHEVDVLICATGFAI
HHCCCCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHCCCEEECCCCCCEEEEEEECCHHH
WDHMPAFPTTGRGGKDLKQFFQTNRYQAYQGVSIPDYPNFFLVLGPYGYVFGPYHMLIES
HHCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCHHHCCHHHEEEC
TTTHAARVIAETKKRGATTAEVKQDVHDAYFHKMHERNKNHLWLSPACSTANTYYIDNHG
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCC
DSPFRPGGFGEMYFHNKYFRLDNYRYGTETIAPAVESAPKRKRNKEVVR
CCCCCCCCCCCEEEECEEEEECCEECCCHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure 
TSARVRDGEARGLRQGVPDHEVVIIGAGFGGIGAGVELTKRGVHDFVILEKWGAAGGTW
CCCCCCCCHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCC
HANKYPGVAVDIPTFIYSFSYDQKTTWSKFFTPGDELEQYANELVDKHELRGKIRFNTRV
CCCCCCCEEEECCCEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCEEEECHHH
VRQDFDESQNIWVLTLEGGGVVTGRHVIAATGGLEQPKLPEIDGIDSYKGVLMHTALWDK
HHHCCCCCCCEEEEEECCCCEEECCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
DIELAGKRVAVIGTGATSLQMVPEIAPQVGHLTVFQRTPIYVGPKPDWRYNGFWRALFGF
CHHHCCCEEEEEECCCHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCCCCCHHHHHHCC
PGVAKAIRHAINLSTAYIPDAVFKLDSKYAESVSMALANRIRAWMRTQVDDPVTREKLLP
CHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCC
YYGFGCKRPSFSNTYLKTFNRPNVALVTEPIQRITEKGLLTADGVEHEVDVLICATGFAI
HHCCCCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHCCCEEECCCCCCEEEEEEECCHHH
WDHMPAFPTTGRGGKDLKQFFQTNRYQAYQGVSIPDYPNFFLVLGPYGYVFGPYHMLIES
HHCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCHHHCCHHHEEEC
TTTHAARVIAETKKRGATTAEVKQDVHDAYFHKMHERNKNHLWLSPACSTANTYYIDNHG
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCC
DSPFRPGGFGEMYFHNKYFRLDNYRYGTETIAPAVESAPKRKRNKEVVR
CCCCCCCCCCCEEEECEEEEECCEECCCHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11322873 [H]