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The map label for this gene is 169627473

Identifier: 169627473

GI number: 169627473

Start: 364765

End: 366015

Strand: Direct

Name: 169627473

Synonym: MAB_0369

Alternate gene names: NA

Gene position: 364765-366015 (Clockwise)

Preceding gene: 169627472

Following gene: 169627474

Centisome position: 7.2

GC content: 66.35

Gene sequence:

>1251_bases
ATGGCCACCACGGCGACGGAGGCTGACACAGGCCGAACCCTGTCGGGGCACGACGAGGCTGCCGAGCACATCGCTCGGCA
GGCCTTCGCCGACGCGCAGGTCGGTGCGGTGGGTCTGGAGCTGGAATCTCATACCGTCGAGCTGACTGCTCCGCACCGCA
GAATCACCTGGAACCGGTTGCGTGAGGTCGGGGACTCGGTTCCTGACCTGCCCGGGTCCAGTGCGATTACCTTTGAGCCC
GGCGGTGCCGTCGAGCTCTCCGGCCCCCCATGTTCCGACGTCTGGTCCGCCATCTCCTCGATGCGGGCCGACCACGAGAT
CTTGACCGCGGTGTATCGCGGTGCCGGGATCGCGCTCGCCAGTTTGGGCACCGACCCGCTGCGCAGACCCGAGCGCGTGA
ATCCCGGAGCCCGGTATGTGGCGATGGAGAGGCATTTCGGCGCCGCCGGATATGGCGAGACCGCGCTGCAGATGATGACC
TGCACGGCGTCACTGCAGGTCAATGTGCAATCCGGGACTCCGCGCCAGTGGCGGGACAGGTTTGTCCTGGCGCAGCGGAT
CGGTCCGACGATGGCGGCGCTATCGGCATCCTCACCGATGCTCACCGGTCGCCCTACCGGTCGCCGGAACACCCGGCAAT
GGATCTGGGACAACTTGGATCCGCGGCGCTGCGCCCCCGTCGAGATCGGTGTCGACCCCACCGAATCCTGGGTCGAATAT
GCCCTGCGGGCGCCGGTTATGTTGGTGCGAAACAAGGATGGCGCCGACGCGGTTGTCACCCGTGTGCCCTTCCAATCCTG
GGTCGACGGGACTATGCCGCTTGCGGGACGCGCCCCGACGACCGAAGACCTCGACTACCACCTGACCACATTGTTTCCCC
CGGTACGGCCGCGCCGCTGGTTGGAACTGCGCTATCTGGACGCCGCACCGGACTGGTGGTGGCCCGCCCTGGCGTTTACG
GCGGTCGCCGCGTTGGATGATCCGCAGGTCGCGGATATTGCGGCGGAGATAGCAGAACCGGTGGGCAACGCATGGGGCGT
GGCCGCACGAATCGGCCTGGAGGATCCTGCCCTGCACGCCGCCGCGCACAGACTGGTGTCGGCCGCGTGCGCGGTGGCGC
CGCCCGAGTTGGCCACCGACATGGAGTTCTTGCTCGAACGAGTCGAGCAAGGTCGATGTCCGGCAGATGATTTCATCGAC
AATGTCACAGAATACGGTGTGGAGAAGGCCTTTTCGGGGGCAATCGGATGA

Upstream 100 bases:

>100_bases
CCAACAGCTACATGAACGCCGAGACAATTCGCATCGACGGTGGGGCCCGGCTCCAGCCGAAGTAGAGCAACTTGTCGGTG
GCCTATGGAACATTGGAGAG

Downstream 100 bases:

>100_bases
GTCGTGATGAGTTGGCGCGCGACCTTGAGGCGGCGCGGATGCGGACGCTGACGATTACCGACCACGACGACGCCGAGTTG
CATCGCCAATACGACCCGCT

Product: glutamate--cysteine ligase GshA

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase GSHA; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MATTATEADTGRTLSGHDEAAEHIARQAFADAQVGAVGLELESHTVELTAPHRRITWNRLREVGDSVPDLPGSSAITFEP
GGAVELSGPPCSDVWSAISSMRADHEILTAVYRGAGIALASLGTDPLRRPERVNPGARYVAMERHFGAAGYGETALQMMT
CTASLQVNVQSGTPRQWRDRFVLAQRIGPTMAALSASSPMLTGRPTGRRNTRQWIWDNLDPRRCAPVEIGVDPTESWVEY
ALRAPVMLVRNKDGADAVVTRVPFQSWVDGTMPLAGRAPTTEDLDYHLTTLFPPVRPRRWLELRYLDAAPDWWWPALAFT
AVAALDDPQVADIAAEIAEPVGNAWGVAARIGLEDPALHAAAHRLVSAACAVAPPELATDMEFLLERVEQGRCPADDFID
NVTEYGVEKAFSGAIG

Sequences:

>Translated_416_residues
MATTATEADTGRTLSGHDEAAEHIARQAFADAQVGAVGLELESHTVELTAPHRRITWNRLREVGDSVPDLPGSSAITFEP
GGAVELSGPPCSDVWSAISSMRADHEILTAVYRGAGIALASLGTDPLRRPERVNPGARYVAMERHFGAAGYGETALQMMT
CTASLQVNVQSGTPRQWRDRFVLAQRIGPTMAALSASSPMLTGRPTGRRNTRQWIWDNLDPRRCAPVEIGVDPTESWVEY
ALRAPVMLVRNKDGADAVVTRVPFQSWVDGTMPLAGRAPTTEDLDYHLTTLFPPVRPRRWLELRYLDAAPDWWWPALAFT
AVAALDDPQVADIAAEIAEPVGNAWGVAARIGLEDPALHAAAHRLVSAACAVAPPELATDMEFLLERVEQGRCPADDFID
NVTEYGVEKAFSGAIG
>Mature_415_residues
ATTATEADTGRTLSGHDEAAEHIARQAFADAQVGAVGLELESHTVELTAPHRRITWNRLREVGDSVPDLPGSSAITFEPG
GAVELSGPPCSDVWSAISSMRADHEILTAVYRGAGIALASLGTDPLRRPERVNPGARYVAMERHFGAAGYGETALQMMTC
TASLQVNVQSGTPRQWRDRFVLAQRIGPTMAALSASSPMLTGRPTGRRNTRQWIWDNLDPRRCAPVEIGVDPTESWVEYA
LRAPVMLVRNKDGADAVVTRVPFQSWVDGTMPLAGRAPTTEDLDYHLTTLFPPVRPRRWLELRYLDAAPDWWWPALAFTA
VAALDDPQVADIAAEIAEPVGNAWGVAARIGLEDPALHAAAHRLVSAACAVAPPELATDMEFLLERVEQGRCPADDFIDN
VTEYGVEKAFSGAIG

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 45031; Mature: 44900

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTATEADTGRTLSGHDEAAEHIARQAFADAQVGAVGLELESHTVELTAPHRRITWNRL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEECCCCCCCHHHH
REVGDSVPDLPGSSAITFEPGGAVELSGPPCSDVWSAISSMRADHEILTAVYRGAGIALA
HHHHCCCCCCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEEE
SLGTDPLRRPERVNPGARYVAMERHFGAAGYGETALQMMTCTASLQVNVQSGTPRQWRDR
ECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHEEEEEEEEEECCCCCHHHHHH
FVLAQRIGPTMAALSASSPMLTGRPTGRRNTRQWIWDNLDPRRCAPVEIGVDPTESWVEY
HHHHHHCCCHHHHHCCCCCCEECCCCCCCCCHHHHCCCCCCCCCCCEEECCCCHHHHHHH
ALRAPVMLVRNKDGADAVVTRVPFQSWVDGTMPLAGRAPTTEDLDYHLTTLFPPVRPRRW
HHHCCEEEEECCCCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCEEEHHHCCCCCCCCE
LELRYLDAAPDWWWPALAFTAVAALDDPQVADIAAEIAEPVGNAWGVAARIGLEDPALHA
EEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHH
AAHRLVSAACAVAPPELATDMEFLLERVEQGRCPADDFIDNVTEYGVEKAFSGAIG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATTATEADTGRTLSGHDEAAEHIARQAFADAQVGAVGLELESHTVELTAPHRRITWNRL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEECCCCCCCHHHH
REVGDSVPDLPGSSAITFEPGGAVELSGPPCSDVWSAISSMRADHEILTAVYRGAGIALA
HHHHCCCCCCCCCCEEEECCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEEE
SLGTDPLRRPERVNPGARYVAMERHFGAAGYGETALQMMTCTASLQVNVQSGTPRQWRDR
ECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHEEEEEEEEEECCCCCHHHHHH
FVLAQRIGPTMAALSASSPMLTGRPTGRRNTRQWIWDNLDPRRCAPVEIGVDPTESWVEY
HHHHHHCCCHHHHHCCCCCCEECCCCCCCCCHHHHCCCCCCCCCCCEEECCCCHHHHHHH
ALRAPVMLVRNKDGADAVVTRVPFQSWVDGTMPLAGRAPTTEDLDYHLTTLFPPVRPRRW
HHHCCEEEEECCCCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCEEEHHHCCCCCCCCE
LELRYLDAAPDWWWPALAFTAVAALDDPQVADIAAEIAEPVGNAWGVAARIGLEDPALHA
EEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHH
AAHRLVSAACAVAPPELATDMEFLLERVEQGRCPADDFIDNVTEYGVEKAFSGAIG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA