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The map label for this gene is ask [H]

Identifier: 169627447

GI number: 169627447

Start: 340079

End: 341344

Strand: Direct

Name: ask [H]

Synonym: MAB_0343

Alternate gene names: 169627447

Gene position: 340079-341344 (Clockwise)

Preceding gene: 169627445

Following gene: 169627448

Centisome position: 6.71

GC content: 64.38

Gene sequence:

>1266_bases
GTGGCGCTCGTCGTCCAAAAGTACGGCGGATCGTCGGTCGCAACGGCCGAACGTATCCGCAGGGTCGCCGAACGCATCGT
CGAGACCAAGAAGAATGGCAACGACGTCGTCGTTGTCGTCTCGGCAATGGGTGACACCACCGACGAGCTGCTTGACCTGG
CCCAACAGGTCTGCCCGGCGCCTCCGCCGCGTGAGCTGGACATGCTCCTGACAGCGGGTGAGCGGATGTCCAATGCCCTG
GTCGCGATGGCCATCCACTCGCTGGGAGCCTCGGCGCGTTCGTTTACCGGCTCACAGGCCGGCGTGATCACCACCGGCAA
GCACGGCAGCGCCAAGATCATCGACGTCACGCCCGGCCGTCTGCGCACCGCGCTCGACGAGGGCCAGGTTGTCCTGGTCG
CCGGATTCCAGGGTGTGAGCCAGGACAGCAAGGATGTGACGACACTCGGGCGGGGTGGCTCCGATACCACCGCCGTCGCG
CTGGCCGCCGCGCTGAACGCCGATGTGTGCGAGATCTACACCGACGTCGACGGGGTCTTCACCGCCGATCCGCGGATCGT
GCCGAACGCGGCCCGGCTCGACAAGGTCAGTTTCGAGGAAATGCTGGAGATGGCGGCGGCCGGGGCAAAGGTGCTCATGC
TGCGCTGTGTGGAATACGCCCGGCGATACAACGTGCCGGTTCACGTGCGCTCGTCGTATACGGACAAGCCCGGCACGATC
GTCAGCGGATCAATGGAGGACATTCCCGTGGAAGAAGCAATTCTCACCGGTGTCGCGCACGATCGCGGCGAGGCCAAGGT
CACTGTCGTCGGCATTCCCGACGTGCCCGGATATGCCGCCAAGGTATTCCGCGCGGTCGCCGACGCCGAAATCAACATCG
ACATGGTGCTGCAGAACGTCTCTAAGGTCGAGGACGGCAAGACCGACATCACCTTCACCTGCCCCAAGGAGAACGGTCCC
ACCGCCGTGGAGAAGCTGGACTCGTTGAAGAATGAGATCGGCTTCTCGCAGGTTCTTTACGACGACCACATCGGCAAGGT
GTCGCTGGTTGGTGCGGGTATGCGTAGCCACCCCGGCGTCACCGCCACCTTCTGCGAGGCGTTGGCCGAGGCCGGCGTGA
ACATCGAACTCATCTCGACGTCCGAGATCCGGATCTCGGTGTTGGTGAAAGACGACGAGCTGGACGCCGCCGTGCAGGCG
ATCCACAACGCCTTCGATCTCGGGAGCGAAGAGGAAGCCACCGTGTACGCGGGGACCGGGCGCTAG

Upstream 100 bases:

>100_bases
GGTGGGGTTGCCCATGCGGGCCAGGTTATCGAGTTGTCCGACCCTCTATCCTGATGAACGGGGTCTGACCGATCTTGACC
GAACTGCAGGAAGGTATGCC

Downstream 100 bases:

>100_bases
GGCGAGCGAAGCGACGGGAGATAGATATGACCTCCATTGGTGTAGTCGGTGCCACCGGGCAGGTGGGTGCCGTCATGCGG
AAGCTTCTGGAAGAGCGTGA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase; ASK [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MALVVQKYGGSSVATAERIRRVAERIVETKKNGNDVVVVVSAMGDTTDELLDLAQQVCPAPPPRELDMLLTAGERMSNAL
VAMAIHSLGASARSFTGSQAGVITTGKHGSAKIIDVTPGRLRTALDEGQVVLVAGFQGVSQDSKDVTTLGRGGSDTTAVA
LAAALNADVCEIYTDVDGVFTADPRIVPNAARLDKVSFEEMLEMAAAGAKVLMLRCVEYARRYNVPVHVRSSYTDKPGTI
VSGSMEDIPVEEAILTGVAHDRGEAKVTVVGIPDVPGYAAKVFRAVADAEINIDMVLQNVSKVEDGKTDITFTCPKENGP
TAVEKLDSLKNEIGFSQVLYDDHIGKVSLVGAGMRSHPGVTATFCEALAEAGVNIELISTSEIRISVLVKDDELDAAVQA
IHNAFDLGSEEEATVYAGTGR

Sequences:

>Translated_421_residues
MALVVQKYGGSSVATAERIRRVAERIVETKKNGNDVVVVVSAMGDTTDELLDLAQQVCPAPPPRELDMLLTAGERMSNAL
VAMAIHSLGASARSFTGSQAGVITTGKHGSAKIIDVTPGRLRTALDEGQVVLVAGFQGVSQDSKDVTTLGRGGSDTTAVA
LAAALNADVCEIYTDVDGVFTADPRIVPNAARLDKVSFEEMLEMAAAGAKVLMLRCVEYARRYNVPVHVRSSYTDKPGTI
VSGSMEDIPVEEAILTGVAHDRGEAKVTVVGIPDVPGYAAKVFRAVADAEINIDMVLQNVSKVEDGKTDITFTCPKENGP
TAVEKLDSLKNEIGFSQVLYDDHIGKVSLVGAGMRSHPGVTATFCEALAEAGVNIELISTSEIRISVLVKDDELDAAVQA
IHNAFDLGSEEEATVYAGTGR
>Mature_420_residues
ALVVQKYGGSSVATAERIRRVAERIVETKKNGNDVVVVVSAMGDTTDELLDLAQQVCPAPPPRELDMLLTAGERMSNALV
AMAIHSLGASARSFTGSQAGVITTGKHGSAKIIDVTPGRLRTALDEGQVVLVAGFQGVSQDSKDVTTLGRGGSDTTAVAL
AAALNADVCEIYTDVDGVFTADPRIVPNAARLDKVSFEEMLEMAAAGAKVLMLRCVEYARRYNVPVHVRSSYTDKPGTIV
SGSMEDIPVEEAILTGVAHDRGEAKVTVVGIPDVPGYAAKVFRAVADAEINIDMVLQNVSKVEDGKTDITFTCPKENGPT
AVEKLDSLKNEIGFSQVLYDDHIGKVSLVGAGMRSHPGVTATFCEALAEAGVNIELISTSEIRISVLVKDDELDAAVQAI
HNAFDLGSEEEATVYAGTGR

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=467, Percent_Identity=28.9079229122056, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1790455, Length=461, Percent_Identity=27.3318872017354, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1790376, Length=308, Percent_Identity=24.6753246753247, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320893, Length=378, Percent_Identity=25.3968253968254, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44373; Mature: 44242

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00324 ASPARTOKINASE ; PS00302 IF5A_HYPUSINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVVQKYGGSSVATAERIRRVAERIVETKKNGNDVVVVVSAMGDTTDELLDLAQQVCPA
CEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
PPPRELDMLLTAGERMSNALVAMAIHSLGASARSFTGSQAGVITTGKHGSAKIIDVTPGR
CCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEECCCCCEEEEECCCHH
LRTALDEGQVVLVAGFQGVSQDSKDVTTLGRGGSDTTAVALAAALNADVCEIYTDVDGVF
HHHHHCCCCEEEEECCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCHHEEEECCCCEE
TADPRIVPNAARLDKVSFEEMLEMAAAGAKVLMLRCVEYARRYNVPVHVRSSYTDKPGTI
ECCCCCCCCHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCE
VSGSMEDIPVEEAILTGVAHDRGEAKVTVVGIPDVPGYAAKVFRAVADAEINIDMVLQNV
EECCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHH
SKVEDGKTDITFTCPKENGPTAVEKLDSLKNEIGFSQVLYDDHIGKVSLVGAGMRSHPGV
HHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCCCCC
TATFCEALAEAGVNIELISTSEIRISVLVKDDELDAAVQAIHNAFDLGSEEEATVYAGTG
HHHHHHHHHHCCCEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCEEEECCC
R
C
>Mature Secondary Structure 
ALVVQKYGGSSVATAERIRRVAERIVETKKNGNDVVVVVSAMGDTTDELLDLAQQVCPA
EEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCC
PPPRELDMLLTAGERMSNALVAMAIHSLGASARSFTGSQAGVITTGKHGSAKIIDVTPGR
CCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEECCCCCEEEEECCCHH
LRTALDEGQVVLVAGFQGVSQDSKDVTTLGRGGSDTTAVALAAALNADVCEIYTDVDGVF
HHHHHCCCCEEEEECCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCHHEEEECCCCEE
TADPRIVPNAARLDKVSFEEMLEMAAAGAKVLMLRCVEYARRYNVPVHVRSSYTDKPGTI
ECCCCCCCCHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCE
VSGSMEDIPVEEAILTGVAHDRGEAKVTVVGIPDVPGYAAKVFRAVADAEINIDMVLQNV
EECCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHH
SKVEDGKTDITFTCPKENGPTAVEKLDSLKNEIGFSQVLYDDHIGKVSLVGAGMRSHPGV
HHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCCCCC
TATFCEALAEAGVNIELISTSEIRISVLVKDDELDAAVQAIHNAFDLGSEEEATVYAGTG
HHHHHHHHHHCCCEEEEEECCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCEEEECCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7910936 [H]