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The map label for this gene is fadD26 [H]

Identifier: 169627284

GI number: 169627284

Start: 176517

End: 178430

Strand: Direct

Name: fadD26 [H]

Synonym: MAB_0179

Alternate gene names: 169627284

Gene position: 176517-178430 (Clockwise)

Preceding gene: 169627283

Following gene: 169627285

Centisome position: 3.48

GC content: 64.94

Gene sequence:

>1914_bases
ATGGCGTTCGACAACCCGTTCCTTGACTCGGCGGGTCATATCAAGTTCCCGGAGGACGGGAGCATCGTCGGGCACGTTGA
GGGCTTCGCGCAGTCACAGGGTGAGAGCCTGGCGTACCGTTTCCTGGACTTTTCCACGGAGCGTGATGGCGCGTATATCG
ACATCACGTGGGCGCAGTTCGGTGCGCGTAACCGTGCGGTGGCGGCGCGTCTGCAGCAGGTGACCAAGCCGGGTGACCGT
GTGGCCATCTTGGCTCCGCAGAGCCTTGATTACTTGGTGGCACATTTCGGTGCGCTGTACGCCGGCGCGATTTCGGTGCC
GTTGTTCGATCCGAGCGAGGCTGGTCATGCCGGGCGCCTGCATGCGGTGCTCGATGATTGCCAGCCTTCGGCGGTGTTGA
CCACCACCGATTGTGCCGAGGGGGTGCGCAAGTTCTTCCGCAATCGCCCGCCCAAGGAGCGCCCCCGTGTCATCGCGGTC
GATGCGATTCCCAACGATGTGGGGTCGACCTGGGTCGAGCCGGTGGCGACCAAGGACACCATCGCGTATCTGCAGTACAC
CTCGGGCTCGACCCGGGCCCCCGCCGGTGTGCAGATCACTCACCTCAATGTGGCCACCAACCTGCTGCAGCTGATCGACG
CGCTGTCCGCGCAAGAAGGCGCGCAGGGACACGAGGCGCTGCGTGGCTGCACCTGGCTGCCGTTCTTCCATGACATGGGC
TTGATCACCGTGATGGTGCCGTCGATGCTCGGCAAGCACATGACCGTGATGAGTCCGGCGGCGTTTATTCGCCGTCCACT
GCGCTGGTTGCGGGAAATGGCCGTCAAGGGCGAGGACCGTGGTGGTAGCTTCTCGGCGCTGCCGAACTTCGCCTTCGAGC
ATTGCGCGTTGCGCGGTGTGCCCAAGGAGGGCGAACCGCCGCTGGACCTGTCGAACATCCACTCGATCATCAATGGGTCC
GAGCCGGTGTCGACGGCGTCGCTGAAGAAGTTCTGCGACGCCTTCGAACCGTACGGTTTTGATCCCAAGGCGATTCACCC
CTCGTACGGCATGGCCGAGGCCACCTTGTTCGTCTCCTCGACCATCTGGAACACCGAACCGGCGCGGGTGCTGCACGTGG
ACCGCACCGAGCTCAATGCCGGGCGCATCGTGCAGGTGGCCCCGGGCGCAGAGAACTCGCTGACTCAGGTCTCCAGTGGC
CGCATGGCCCGCGATGAGTGGGCGGTGATCGTCGACGGCGAGAGTGCCTCGGAGGTGCCCGACGGTCAGATCGGCCAGAT
CTGGTTGCACGGCAACAACATTGGCTCGGGCTACTGGGGCCGCCCGGAAGAGACCCGCGAAGCGTTCCAGAACACCCTCA
AATCACGCATCCCGGCATCGCATGCCGAGGGCGCTCCGGACGACGCCAACTGGCTCAACACCGGTGACCTCGGAGTATGG
GTCGATGGCGAGCTGTACATCACCGGTCGCGTCAAGGACCTGATCATCGTCGACGGCCGCAACCACTACCCCCAAGACAT
CGAATACACCGCACAAGAAGCCAACAAGGCACTGCGCCCGGGATATGTCGCGGCCTTCTCGGTGCCGGCCAACCAGTTGC
CGCAGGTGGTTTTCGACAATCCGCACGCCGGTTTGAAATACGACCCGGAGGATTCCTCCGAGCAGCTGGTCATCATCGGC
GAGCGCAGCGTGGGCGGCCACAAGTCCGACAACCAAGCTGTCGGTGACGACGTGCGCGCCGCGGTCGCGGTACGCCACGG
CGTCACCGTGCGCGATGTGCTGCTGGTCCCGGCGGGCTCGATCGCCCGGACCTCCAGCGGCAAGATCGCCCGCAGCGCCG
CTCGAACCAGCTACCTGGACGGCAGCCTGCGCGGCGGCTACCAGCAGACCGCATTCCCCGACGACCGGCAGTAG

Upstream 100 bases:

>100_bases
TCATCGAGGATTTGACGTTCGCCCCGATGTCATGTCGGCGTGCACGAACTGTTCAGGCCCGCGAACCGCGCATCCGCGCC
GTCACAGGAGAGAAGTACAC

Downstream 100 bases:

>100_bases
GCGATCCAGCATCGCCTCGCCATCCGATACTCCGGAGATAGGCCTCGAACATGACTGATACTCAACAGGATTCGACACCA
GAGACCAGCCTGGAAACCCC

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MAFDNPFLDSAGHIKFPEDGSIVGHVEGFAQSQGESLAYRFLDFSTERDGAYIDITWAQFGARNRAVAARLQQVTKPGDR
VAILAPQSLDYLVAHFGALYAGAISVPLFDPSEAGHAGRLHAVLDDCQPSAVLTTTDCAEGVRKFFRNRPPKERPRVIAV
DAIPNDVGSTWVEPVATKDTIAYLQYTSGSTRAPAGVQITHLNVATNLLQLIDALSAQEGAQGHEALRGCTWLPFFHDMG
LITVMVPSMLGKHMTVMSPAAFIRRPLRWLREMAVKGEDRGGSFSALPNFAFEHCALRGVPKEGEPPLDLSNIHSIINGS
EPVSTASLKKFCDAFEPYGFDPKAIHPSYGMAEATLFVSSTIWNTEPARVLHVDRTELNAGRIVQVAPGAENSLTQVSSG
RMARDEWAVIVDGESASEVPDGQIGQIWLHGNNIGSGYWGRPEETREAFQNTLKSRIPASHAEGAPDDANWLNTGDLGVW
VDGELYITGRVKDLIIVDGRNHYPQDIEYTAQEANKALRPGYVAAFSVPANQLPQVVFDNPHAGLKYDPEDSSEQLVIIG
ERSVGGHKSDNQAVGDDVRAAVAVRHGVTVRDVLLVPAGSIARTSSGKIARSAARTSYLDGSLRGGYQQTAFPDDRQ

Sequences:

>Translated_637_residues
MAFDNPFLDSAGHIKFPEDGSIVGHVEGFAQSQGESLAYRFLDFSTERDGAYIDITWAQFGARNRAVAARLQQVTKPGDR
VAILAPQSLDYLVAHFGALYAGAISVPLFDPSEAGHAGRLHAVLDDCQPSAVLTTTDCAEGVRKFFRNRPPKERPRVIAV
DAIPNDVGSTWVEPVATKDTIAYLQYTSGSTRAPAGVQITHLNVATNLLQLIDALSAQEGAQGHEALRGCTWLPFFHDMG
LITVMVPSMLGKHMTVMSPAAFIRRPLRWLREMAVKGEDRGGSFSALPNFAFEHCALRGVPKEGEPPLDLSNIHSIINGS
EPVSTASLKKFCDAFEPYGFDPKAIHPSYGMAEATLFVSSTIWNTEPARVLHVDRTELNAGRIVQVAPGAENSLTQVSSG
RMARDEWAVIVDGESASEVPDGQIGQIWLHGNNIGSGYWGRPEETREAFQNTLKSRIPASHAEGAPDDANWLNTGDLGVW
VDGELYITGRVKDLIIVDGRNHYPQDIEYTAQEANKALRPGYVAAFSVPANQLPQVVFDNPHAGLKYDPEDSSEQLVIIG
ERSVGGHKSDNQAVGDDVRAAVAVRHGVTVRDVLLVPAGSIARTSSGKIARSAARTSYLDGSLRGGYQQTAFPDDRQ
>Mature_636_residues
AFDNPFLDSAGHIKFPEDGSIVGHVEGFAQSQGESLAYRFLDFSTERDGAYIDITWAQFGARNRAVAARLQQVTKPGDRV
AILAPQSLDYLVAHFGALYAGAISVPLFDPSEAGHAGRLHAVLDDCQPSAVLTTTDCAEGVRKFFRNRPPKERPRVIAVD
AIPNDVGSTWVEPVATKDTIAYLQYTSGSTRAPAGVQITHLNVATNLLQLIDALSAQEGAQGHEALRGCTWLPFFHDMGL
ITVMVPSMLGKHMTVMSPAAFIRRPLRWLREMAVKGEDRGGSFSALPNFAFEHCALRGVPKEGEPPLDLSNIHSIINGSE
PVSTASLKKFCDAFEPYGFDPKAIHPSYGMAEATLFVSSTIWNTEPARVLHVDRTELNAGRIVQVAPGAENSLTQVSSGR
MARDEWAVIVDGESASEVPDGQIGQIWLHGNNIGSGYWGRPEETREAFQNTLKSRIPASHAEGAPDDANWLNTGDLGVWV
DGELYITGRVKDLIIVDGRNHYPQDIEYTAQEANKALRPGYVAAFSVPANQLPQVVFDNPHAGLKYDPEDSSEQLVIIGE
RSVGGHKSDNQAVGDDVRAAVAVRHGVTVRDVLLVPAGSIARTSSGKIARSAARTSYLDGSLRGGYQQTAFPDDRQ

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI55749758, Length=613, Percent_Identity=24.9592169657423, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI44888818, Length=518, Percent_Identity=23.7451737451737, Blast_Score=108, Evalue=2e-23,
Organism=Escherichia coli, GI1788107, Length=554, Percent_Identity=27.9783393501805, Blast_Score=130, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI32563687, Length=463, Percent_Identity=25.7019438444924, Blast_Score=102, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI193202679, Length=647, Percent_Identity=22.8748068006182, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24654656, Length=401, Percent_Identity=23.4413965087282, Blast_Score=98, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 68984; Mature: 68853

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFDNPFLDSAGHIKFPEDGSIVGHVEGFAQSQGESLAYRFLDFSTERDGAYIDITWAQF
CCCCCCCCCCCCCEECCCCCCEEEECCHHHHCCCCCEEEEEEECCCCCCCCEEEEEHHHH
GARNRAVAARLQQVTKPGDRVAILAPQSLDYLVAHFGALYAGAISVPLFDPSEAGHAGRL
CCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCEE
HAVLDDCQPSAVLTTTDCAEGVRKFFRNRPPKERPRVIAVDAIPNDVGSTWVEPVATKDT
HHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCCE
IAYLQYTSGSTRAPAGVQITHLNVATNLLQLIDALSAQEGAQGHEALRGCTWLPFFHDMG
EEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHCCEECHHHHHCC
LITVMVPSMLGKHMTVMSPAAFIRRPLRWLREMAVKGEDRGGSFSALPNFAFEHCALRGV
HHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCC
PKEGEPPLDLSNIHSIINGSEPVSTASLKKFCDAFEPYGFDPKAIHPSYGMAEATLFVSS
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEE
TIWNTEPARVLHVDRTELNAGRIVQVAPGAENSLTQVSSGRMARDEWAVIVDGESASEVP
EECCCCCCEEEEEECCCCCCCCEEEECCCCCCCHHHHHCCCCCCCCEEEEECCCCCCCCC
DGQIGQIWLHGNNIGSGYWGRPEETREAFQNTLKSRIPASHAEGAPDDANWLNTGDLGVW
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
VDGELYITGRVKDLIIVDGRNHYPQDIEYTAQEANKALRPGYVAAFSVPANQLPQVVFDN
ECCEEEEEECEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHHCCCEEECC
PHAGLKYDPEDSSEQLVIIGERSVGGHKSDNQAVGDDVRAAVAVRHGVTVRDVLLVPAGS
CCCCCEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCC
IARTSSGKIARSAARTSYLDGSLRGGYQQTAFPDDRQ
CEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AFDNPFLDSAGHIKFPEDGSIVGHVEGFAQSQGESLAYRFLDFSTERDGAYIDITWAQF
CCCCCCCCCCCCEECCCCCCEEEECCHHHHCCCCCEEEEEEECCCCCCCCEEEEEHHHH
GARNRAVAARLQQVTKPGDRVAILAPQSLDYLVAHFGALYAGAISVPLFDPSEAGHAGRL
CCCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCEE
HAVLDDCQPSAVLTTTDCAEGVRKFFRNRPPKERPRVIAVDAIPNDVGSTWVEPVATKDT
HHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCCE
IAYLQYTSGSTRAPAGVQITHLNVATNLLQLIDALSAQEGAQGHEALRGCTWLPFFHDMG
EEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHCCEECHHHHHCC
LITVMVPSMLGKHMTVMSPAAFIRRPLRWLREMAVKGEDRGGSFSALPNFAFEHCALRGV
HHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCC
PKEGEPPLDLSNIHSIINGSEPVSTASLKKFCDAFEPYGFDPKAIHPSYGMAEATLFVSS
CCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEE
TIWNTEPARVLHVDRTELNAGRIVQVAPGAENSLTQVSSGRMARDEWAVIVDGESASEVP
EECCCCCCEEEEEECCCCCCCCEEEECCCCCCCHHHHHCCCCCCCCEEEEECCCCCCCCC
DGQIGQIWLHGNNIGSGYWGRPEETREAFQNTLKSRIPASHAEGAPDDANWLNTGDLGVW
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEE
VDGELYITGRVKDLIIVDGRNHYPQDIEYTAQEANKALRPGYVAAFSVPANQLPQVVFDN
ECCEEEEEECEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCHHHCCCEEECC
PHAGLKYDPEDSSEQLVIIGERSVGGHKSDNQAVGDDVRAAVAVRHGVTVRDVLLVPAGS
CCCCCEECCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCC
IARTSSGKIARSAARTSYLDGSLRGGYQQTAFPDDRQ
CEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]