BacMap

Definition	Mycobacterium abscessus ATCC 19977 chromosome chromosome 1, complete sequence.
Accession	NC_010397
Length	5,067,172

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The map label for this gene is ppiA [H]

Identifier: 169627132

GI number: 169627132

Start: 25003

End: 25539

Strand: Direct

Name: ppiA [H]

Synonym: MAB_0025

Alternate gene names: 169627132

Gene position: 25003-25539 (Clockwise)

Preceding gene: 169627130

Following gene: 169627133

Centisome position: 0.49

GC content: 65.55

Gene sequence:

>537_bases
GTGACTTCACCTACTTCACCGATCCAGACAGCCACCGCAACCCTGCACACCAACCGCGGCGACGTCGTCATCGCGCTCTT
CGGTAACCACGCCCCGAAGACCGTCGCCAACTTCGTCGGCCTGGCCGACGGCAGCAAGGAGTACTCCACCAAGAACGCCA
AGGGCGAGGCCGCTGGCCCGTTCTACGACGGCGCCATCTTCCACCGGGTCATCGACGGATTCATGATCCAGGGCGGTGAC
CCCACCGGCACCGGACGCGGCGGCCCGGGATATCAGTTCGCCGACGAGTTCCACCCCGAGCTGCAGTTCGACAAGCCCTA
CCTGCTGGCCATGGCCAACGCCGGACCGGGCACCAACGGCTCGCAGTTCTTCATCACCGTGGGCAAGACCCCGCACCTGA
ACCGCCGCCACACCATCTTCGGCGAAGTGGTGGACCCCGCCTCACAGCAGGTCGTCGACTCCATCGCCTCCACCGCCGTC
GATCGCGGCGACCGTCCCACCGAGGATGTCGTGATCAACTCGATCACCATCAGCTAG

Upstream 100 bases:

>100_bases
CGCACCGACAGCCATGCCTCGCGGGAAAAGAACCGCGTGCCGGATGGCTGTTGCGAAACATCCGCATGAATGTCTTTTCC
GCGCGGATAAGGTGGTCGTC

Downstream 100 bases:

>100_bases
GTCTCGATGGCTCATCCCGGTCAGGTCCCGGTCTACGGGTGCGCTTGGCACCCGGACCGGGCCACCGCGGTGCGCTGCGT
GCGGTGCGGGCGCCCCGTGT

Product: peptidyl-prolyl cis-trans isomerase

Products: NA

Alternate protein names: PPIase A; Rotamase A [H]

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MTSPTSPIQTATATLHTNRGDVVIALFGNHAPKTVANFVGLADGSKEYSTKNAKGEAAGPFYDGAIFHRVIDGFMIQGGD
PTGTGRGGPGYQFADEFHPELQFDKPYLLAMANAGPGTNGSQFFITVGKTPHLNRRHTIFGEVVDPASQQVVDSIASTAV
DRGDRPTEDVVINSITIS

Sequences:

>Translated_178_residues
MTSPTSPIQTATATLHTNRGDVVIALFGNHAPKTVANFVGLADGSKEYSTKNAKGEAAGPFYDGAIFHRVIDGFMIQGGD
PTGTGRGGPGYQFADEFHPELQFDKPYLLAMANAGPGTNGSQFFITVGKTPHLNRRHTIFGEVVDPASQQVVDSIASTAV
DRGDRPTEDVVINSITIS
>Mature_177_residues
TSPTSPIQTATATLHTNRGDVVIALFGNHAPKTVANFVGLADGSKEYSTKNAKGEAAGPFYDGAIFHRVIDGFMIQGGDP
TGTGRGGPGYQFADEFHPELQFDKPYLLAMANAGPGTNGSQFFITVGKTPHLNRRHTIFGEVVDPASQQVVDSIASTAVD
RGDRPTEDVVINSITIS

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI24308049, Length=170, Percent_Identity=47.0588235294118, Blast_Score=144, Evalue=6e-35,
Organism=Homo sapiens, GI19557636, Length=170, Percent_Identity=44.1176470588235, Blast_Score=138, Evalue=3e-33,
Organism=Homo sapiens, GI7706339, Length=172, Percent_Identity=44.7674418604651, Blast_Score=138, Evalue=3e-33,
Organism=Homo sapiens, GI64276486, Length=166, Percent_Identity=40.9638554216867, Blast_Score=126, Evalue=9e-30,
Organism=Homo sapiens, GI4758950, Length=157, Percent_Identity=46.4968152866242, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI4505991, Length=160, Percent_Identity=46.25, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI42560244, Length=160, Percent_Identity=42.5, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI22547212, Length=167, Percent_Identity=41.9161676646707, Blast_Score=103, Evalue=6e-23,
Organism=Homo sapiens, GI7657473, Length=167, Percent_Identity=41.9161676646707, Blast_Score=103, Evalue=6e-23,
Organism=Homo sapiens, GI22547215, Length=167, Percent_Identity=41.9161676646707, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI6631100, Length=157, Percent_Identity=40.7643312101911, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI4826932, Length=159, Percent_Identity=41.5094339622642, Blast_Score=99, Evalue=2e-21,
Organism=Homo sapiens, GI5454154, Length=182, Percent_Identity=35.7142857142857, Blast_Score=93, Evalue=1e-19,
Organism=Homo sapiens, GI10863927, Length=130, Percent_Identity=43.8461538461538, Blast_Score=92, Evalue=2e-19,
Organism=Homo sapiens, GI256017253, Length=130, Percent_Identity=42.3076923076923, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI221139811, Length=130, Percent_Identity=42.3076923076923, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI256017251, Length=130, Percent_Identity=42.3076923076923, Blast_Score=89, Evalue=2e-18,
Organism=Homo sapiens, GI5031987, Length=157, Percent_Identity=40.7643312101911, Blast_Score=88, Evalue=4e-18,
Organism=Homo sapiens, GI20911035, Length=174, Percent_Identity=34.4827586206897, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI310129267, Length=139, Percent_Identity=37.410071942446, Blast_Score=87, Evalue=8e-18,
Organism=Homo sapiens, GI310120620, Length=139, Percent_Identity=37.410071942446, Blast_Score=87, Evalue=8e-18,
Organism=Homo sapiens, GI178057341, Length=130, Percent_Identity=41.5384615384615, Blast_Score=86, Evalue=1e-17,
Organism=Homo sapiens, GI209417936, Length=127, Percent_Identity=40.1574803149606, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI30102944, Length=127, Percent_Identity=40.1574803149606, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI219842199, Length=127, Percent_Identity=40.1574803149606, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI169163387, Length=130, Percent_Identity=42.3076923076923, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI150418007, Length=129, Percent_Identity=39.5348837209302, Blast_Score=84, Evalue=5e-17,
Organism=Homo sapiens, GI310110104, Length=139, Percent_Identity=38.1294964028777, Blast_Score=84, Evalue=6e-17,
Organism=Homo sapiens, GI14277126, Length=171, Percent_Identity=35.0877192982456, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI303305009, Length=159, Percent_Identity=39.622641509434, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI169164185, Length=130, Percent_Identity=41.5384615384615, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI169163623, Length=130, Percent_Identity=41.5384615384615, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI310127646, Length=127, Percent_Identity=38.5826771653543, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI310114371, Length=127, Percent_Identity=38.5826771653543, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI310119221, Length=127, Percent_Identity=38.5826771653543, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI5174637, Length=157, Percent_Identity=36.9426751592357, Blast_Score=81, Evalue=6e-16,
Organism=Homo sapiens, GI45439316, Length=130, Percent_Identity=40, Blast_Score=80, Evalue=9e-16,
Organism=Homo sapiens, GI169214050, Length=139, Percent_Identity=35.2517985611511, Blast_Score=77, Evalue=7e-15,
Organism=Homo sapiens, GI310132444, Length=129, Percent_Identity=37.2093023255814, Blast_Score=77, Evalue=8e-15,
Organism=Homo sapiens, GI310113152, Length=129, Percent_Identity=37.2093023255814, Blast_Score=77, Evalue=8e-15,
Organism=Homo sapiens, GI239751853, Length=139, Percent_Identity=35.2517985611511, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI113428306, Length=139, Percent_Identity=35.2517985611511, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI27735045, Length=134, Percent_Identity=35.0746268656716, Blast_Score=75, Evalue=3e-14,
Organism=Homo sapiens, GI310127745, Length=120, Percent_Identity=37.5, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI310119290, Length=120, Percent_Identity=37.5, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI310114540, Length=122, Percent_Identity=36.8852459016393, Blast_Score=71, Evalue=4e-13,
Organism=Escherichia coli, GI1786736, Length=180, Percent_Identity=36.1111111111111, Blast_Score=84, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17506311, Length=170, Percent_Identity=46.4705882352941, Blast_Score=134, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17539496, Length=167, Percent_Identity=43.7125748502994, Blast_Score=130, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71997782, Length=167, Percent_Identity=39.5209580838323, Blast_Score=120, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71980594, Length=169, Percent_Identity=39.6449704142012, Blast_Score=119, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17552780, Length=157, Percent_Identity=49.6815286624204, Blast_Score=118, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17552784, Length=160, Percent_Identity=45, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71980590, Length=158, Percent_Identity=40.5063291139241, Blast_Score=116, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17551282, Length=159, Percent_Identity=44.0251572327044, Blast_Score=115, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17551284, Length=159, Percent_Identity=44.0251572327044, Blast_Score=115, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17506307, Length=157, Percent_Identity=48.4076433121019, Blast_Score=112, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17532641, Length=168, Percent_Identity=38.0952380952381, Blast_Score=103, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17559074, Length=158, Percent_Identity=41.1392405063291, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17566266, Length=131, Percent_Identity=44.2748091603053, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17532643, Length=157, Percent_Identity=38.2165605095541, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17565860, Length=157, Percent_Identity=41.4012738853503, Blast_Score=91, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17539498, Length=130, Percent_Identity=42.3076923076923, Blast_Score=87, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17552782, Length=157, Percent_Identity=39.4904458598726, Blast_Score=83, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI32563659, Length=174, Percent_Identity=28.1609195402299, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115533296, Length=166, Percent_Identity=32.5301204819277, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323246, Length=163, Percent_Identity=41.1042944785276, Blast_Score=110, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320359, Length=159, Percent_Identity=42.7672955974843, Blast_Score=99, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323562, Length=160, Percent_Identity=41.875, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320510, Length=157, Percent_Identity=41.4012738853503, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321848, Length=158, Percent_Identity=42.4050632911392, Blast_Score=95, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6322492, Length=180, Percent_Identity=37.7777777777778, Blast_Score=89, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17986117, Length=172, Percent_Identity=42.4418604651163, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19922912, Length=169, Percent_Identity=45.5621301775148, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI19922376, Length=163, Percent_Identity=39.8773006134969, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24650807, Length=157, Percent_Identity=43.312101910828, Blast_Score=113, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24652460, Length=170, Percent_Identity=37.0588235294118, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI20130249, Length=163, Percent_Identity=46.0122699386503, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21357249, Length=141, Percent_Identity=37.5886524822695, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI45549139, Length=159, Percent_Identity=41.5094339622642, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24664125, Length=157, Percent_Identity=40.7643312101911, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357783, Length=157, Percent_Identity=40.7643312101911, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17136360, Length=161, Percent_Identity=34.7826086956522, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24586038, Length=133, Percent_Identity=41.3533834586466, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17647301, Length=160, Percent_Identity=37.5, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355677, Length=177, Percent_Identity=29.3785310734463, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: 780 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 18821; Mature: 18690

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPTSPIQTATATLHTNRGDVVIALFGNHAPKTVANFVGLADGSKEYSTKNAKGEAAGP
CCCCCCCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
FYDGAIFHRVIDGFMIQGGDPTGTGRGGPGYQFADEFHPELQFDKPYLLAMANAGPGTNG
CCCHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCC
SQFFITVGKTPHLNRRHTIFGEVVDPASQQVVDSIASTAVDRGDRPTEDVVINSITIS
CEEEEEECCCCCCCCCCEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCHHEEEEEEEEC
>Mature Secondary Structure 
TSPTSPIQTATATLHTNRGDVVIALFGNHAPKTVANFVGLADGSKEYSTKNAKGEAAGP
CCCCCCCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
FYDGAIFHRVIDGFMIQGGDPTGTGRGGPGYQFADEFHPELQFDKPYLLAMANAGPGTNG
CCCHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCCCCCC
SQFFITVGKTPHLNRRHTIFGEVVDPASQQVVDSIASTAVDRGDRPTEDVVINSITIS
CEEEEEECCCCCCCCCCEEEHHHCCCHHHHHHHHHHHHHHHCCCCCCHHEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]