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The map label for this gene is amyS [H]

Identifier: 169834400

GI number: 169834400

Start: 1400202

End: 1401656

Strand: Direct

Name: amyS [H]

Synonym: SPH_1513

Alternate gene names: 169834400

Gene position: 1400202-1401656 (Clockwise)

Preceding gene: 169833760

Following gene: 169832988

Centisome position: 62.35

GC content: 45.7

Gene sequence:

>1455_bases
ATGCAAAATCAAACACTCATGCAATACTTTGAATGGTATCTGCCCCACGACGGTCAACACTGGACGCGTCTGGCTGAAAA
TGCTCCACACCTAGCTCATCTGGGGATCAGTCACGTCTGGATGCCACCAGCCTTCAAGGCAACCAACGAAAAAGATGTCG
GCTATGGGGTCTATGACTTATTTGACTTAGGAGAGTTCAACCAAAAAGGGACTGTCCGCACCAAGTATGGTTTCAAAGAA
GACTATCTTCAAGCCATTCAAGCCCTTAAAGCGCAGGGAATTCAACCTATGGCCGATGTGGTTCTCAACCACAAGGCTGC
TGCTGATCACAGGGAAGCCTTTCAAGTTATTGAAGTGGATCCTGTAGACCGTACAGTTGAACTTGGAGAACCCTTCACCA
TCAATGGCTGGACTAGTTTTACCTTCGATGGTCGCCAAGATACCTACAATGACTTCCACTGGCACTGGTACCACTTCACA
GGTACAGACTATGATGCCAAACGCCGTAAGTCTGGGATTTATCTGATCCAAGGGGACAACAAGGGCTGGGCTAACGAGGA
ATTGGTCGATAACGAAAACGGAAACTACGACTACCTCATGTATGCCGACCTAGACTTTAAACATCCTGAAGTCATCCAAA
ACATCTATGACTGGGCTGATTGGTTCATGGAAACGACTGGTGTAGCTGGTTTCCGTTTGGATGCCGTTAAGCATATTGAC
TCTTTCTTTATGCGCAACTTCATCCGCGATATGAAGGAAAAATACGGTGACGATTTCTATGTTTTTGGTGAATTTTGGAA
CCCAGACAAGGAAGCCAATCTGGACTATCTCGAAAAAACGGAAGAACACTTTGACCTTGTCGATGTTCGTCTCCATCAGA
ATCTCTTTGAAGCCAGTCAAGCTGGCGCAAACTATGACCTTCGTGGCATTTTCACAGATAGCCTGGTTGAACTCAAGCCT
GACAAGGCTGTGACTTTTGTCGACAACCACGATACCCAACGAGGACAAGCCCTTGAGTCTACCGTTGAAGAATGGTTCAA
GCCAGCAGCCTATGCCCTCATTTTGTTACGCCAAGACGGCCTTCCATGTGTCTTTTACGGAGACTACTATGGGATTTCAG
GCCAGTATGCTCAAGAAGATTTCAAAGAAATCCTTGACCGCCTCCTAGCCATCCGAAAAGATTTGGCCTATGGAGAACAA
AATGACTACTTTGACCATGCTAACTGTATCGGTTGGGTACGTTCAGGTGCTGAAAATCAATCCCCAATCGCAGTCCTTAT
CTCAAATGACCAAGAAAACAGCAAGTCAATGTTTGTCGGTCAAGAATGGACTAATCAAACCTTTGTAGATTTACTTGGTA
ACCACCAAGGTCAAGTTACAATTGATGAGGAAGGTTATGGACAATTCCCTGTCTCAGCTAGATCCGTAAGTGTCTGGGCA
GTCAATACCATCTAA

Upstream 100 bases:

>100_bases
AGTAGTTAAAATAGTTTCCTTCTATCTTACGCAAACGTTTGCGCTAATAGATACTTTATGATAGAATAAAGAACAAGATT
GACAAGTAAGAGGAAACATT

Downstream 100 bases:

>100_bases
TAGCTCATAATAACCAAGCTAGGTCCAAGCGGATTTGGCTTTTTTGTATTCACAAAAAGACCTACCCAAATGGATAGATC
TTTACTTGATTACAATTTAC

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPAFKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKE
DYLQAIQALKAQGIQPMADVVLNHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDWADWFMETTGVAGFRLDAVKHID
SFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDYLEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKP
DKAVTFVDNHDTQRGQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIRKDLAYGEQ
NDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVDLLGNHQGQVTIDEEGYGQFPVSARSVSVWA
VNTI

Sequences:

>Translated_484_residues
MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPAFKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKE
DYLQAIQALKAQGIQPMADVVLNHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDWADWFMETTGVAGFRLDAVKHID
SFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDYLEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKP
DKAVTFVDNHDTQRGQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIRKDLAYGEQ
NDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVDLLGNHQGQVTIDEEGYGQFPVSARSVSVWA
VNTI
>Mature_484_residues
MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPAFKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKE
DYLQAIQALKAQGIQPMADVVLNHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDWADWFMETTGVAGFRLDAVKHID
SFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDYLEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKP
DKAVTFVDNHDTQRGQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIRKDLAYGEQ
NDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVDLLGNHQGQVTIDEEGYGQFPVSARSVSVWA
VNTI

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=494, Percent_Identity=43.1174089068826, Blast_Score=384, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 56045; Mature: 56045

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPAFKATNEKDVGYGVYDL
CCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEECCCCCCEECCCCCCCCCCCCCCCCHHHH
FDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVLNHKAAADHREAFQVIEVD
HHCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHCCHHHCEEEEEEC
PVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFTGTDYDAKRRKSGIYLIQGDN
CCCCEEECCCCEEECCEEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHCCEEEEECCC
KGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDWADWFMETTGVAGFRLDAVKHID
CCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDYLEKTEEHFDLVDVRLHQNLFEASQ
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQRGQALESTVEEWFKPAAYALILLRQDG
CCCCCEEEEEECCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCC
LPCVFYGDYYGISGQYAQEDFKEILDRLLAIRKDLAYGEQNDYFDHANCIGWVRSGAENQ
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCC
SPIAVLISNDQENSKSMFVGQEWTNQTFVDLLGNHQGQVTIDEEGYGQFPVSARSVSVWA
CCEEEEEECCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEEE
VNTI
EECC
>Mature Secondary Structure
MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPAFKATNEKDVGYGVYDL
CCCHHHHHHHHHHCCCCCHHHHHHHHCCCCEECCCCCCEECCCCCCCCCCCCCCCCHHHH
FDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVLNHKAAADHREAFQVIEVD
HHCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHCCHHHCEEEEEEC
PVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFTGTDYDAKRRKSGIYLIQGDN
CCCCEEECCCCEEECCEEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHCCEEEEECCC
KGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDWADWFMETTGVAGFRLDAVKHID
CCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDYLEKTEEHFDLVDVRLHQNLFEASQ
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQRGQALESTVEEWFKPAAYALILLRQDG
CCCCCEEEEEECCCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCC
LPCVFYGDYYGISGQYAQEDFKEILDRLLAIRKDLAYGEQNDYFDHANCIGWVRSGAENQ
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCC
SPIAVLISNDQENSKSMFVGQEWTNQTFVDLLGNHQGQVTIDEEGYGQFPVSARSVSVWA
CCEEEEEECCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEEE
VNTI
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3924897; 3876333; 3009417; 2999073; 11226887 [H]