Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 169834329

GI number: 169834329

Start: 10531

End: 11808

Strand: Direct

Name: tilS [H]

Synonym: SPH_0011

Alternate gene names: 169834329

Gene position: 10531-11808 (Clockwise)

Preceding gene: 169832420

Following gene: 169833613

Centisome position: 0.47

GC content: 34.12

Gene sequence:

>1278_bases
ATGAGGGAACCAGATTTTTTAAATCATTTTCTCAAGAAGGGATATTTCAAAAAGCATGCTAAGGCGGTTCTAGCTCTTTC
TGGTGGATTAGATTCTATGTTTCTATTTAAGGTATTGTCTACTTATCAAAAAGAGTTAGAGATTGAATTGATTCTGGCTC
ATGTGAATCATAAGCAGAGAATTGAATCAGATTGGGAAGAAAAGGAATTAAGGAAGTTGGCTGCTGAAGCAGAGCTTCCT
ATTTATATCAGCAATTTTTCAGGAGAATTTTCAGAAGCGCGTGCACGAAATTTTCGTTATGATTTTTTTCAAGAGGTTAT
GAAAAAGACAGGTGCGACAGCTTTAGTCACTGCCCACCATGCTGATGATCAGGTGGAAACGATTTTGATGCGCTTGATTC
GAGGAACTCGCTTGCGCTATCTATCAGGAATTAAGGAGAAGCAAGTAGTCGGAGAGATAGAAATCATTCGTCCCTTCTTG
CATTTTCAGAAAAAAGACTTTCCATCAATTTTTCACTTTGAAGATACATCAAATCAGGAGAATCATTATTTTCGAAATCG
TATTCGAAACTCTTACTTACCAGAATTGGAAAAAGAAAATCCTCGATTTAGGGATGCAATCTTAGGCATTGGCAATGAAA
TTTTAGATTATGATTTGGCAATAGCTGAATTATCTAACAATATTAATGTGGAAGATTTACAGCAGTTATTTTCTTACTCT
GAGTCTACACAAAGAGTTTTACTTCAAACTTATCTGAATCGTTTTCCAGATTTGAATCTTACAAAAGCTCAGTTTGCTGA
AGTTCAGCAGATTTTAAAATCTAAAAGCCAGTATCGTCATCCGATTAAAAATGGCTATGAATTGATAAAAGAGTACCAAC
AGTTTCAGATTTGTAAAATCAGTCCGCAGGCTGATGAAGAGGAAGATGAATTTGTGTTACACTATCAAAATCAGGTAGCT
TATCAAGGATATTTATTTTCCTTTGGACTTCCATTAGAAGGTGAATCAATTCAACAAATACCTGTTTCACGTGAAACATC
CATACACATTCGTCATCGAAAAACAGGAGATGTTTTGATTCAAAATGGGCATAGAAAAAAACTCAGACGTTTATTTATTG
ATTTGAAAATCCCTATGGAAAAGAGAAACTCTGCTCTTATTATTGAGCAATTTGGTGAAATTGTCTCAATTTTGGGAATT
GCGACCAATAATTTGAGTAAAAAAACGAAAAATGATATAATGAACACTGTACTTTATATAGAAAAAATAGATAGGTAA

Upstream 100 bases:

>100_bases
TTGTCTATGCAGATTCTCCATTTATTCTTTCTATTTTCACTAAGAATTCTGATTATGATACGATTTCTAAGATAGCCAAG
GATGTTTATGAGGTTCTAAA

Downstream 100 bases:

>100_bases
AAAATGTTAGAAAACGATATTAAAAAAGTCCTCGTTTCACACGATGAAATTACAGAAGCAGCTAAAAAACTAGGTGCTCA
ATTAACTAAAGATTATGCAG

Product: tRNA(Ile)-lysidine synthase (tRNA(Ile)-lysidinesynthetase) (tRNA(Ile)-2-lysyl-cytidine synthase)

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQKELEIELILAHVNHKQRIESDWEEKELRKLAAEAELP
IYISNFSGEFSEARARNFRYDFFQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYDLAIAELSNNINVEDLQQLFSYS
ESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKSQYRHPIKNGYELIKEYQQFQICKISPQADEEEDEFVLHYQNQVA
YQGYLFSFGLPLEGESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFGEIVSILGI
ATNNLSKKTKNDIMNTVLYIEKIDR

Sequences:

>Translated_425_residues
MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQKELEIELILAHVNHKQRIESDWEEKELRKLAAEAELP
IYISNFSGEFSEARARNFRYDFFQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYDLAIAELSNNINVEDLQQLFSYS
ESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKSQYRHPIKNGYELIKEYQQFQICKISPQADEEEDEFVLHYQNQVA
YQGYLFSFGLPLEGESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFGEIVSILGI
ATNNLSKKTKNDIMNTVLYIEKIDR
>Mature_425_residues
MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQKELEIELILAHVNHKQRIESDWEEKELRKLAAEAELP
IYISNFSGEFSEARARNFRYDFFQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYDLAIAELSNNINVEDLQQLFSYS
ESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKSQYRHPIKNGYELIKEYQQFQICKISPQADEEEDEFVLHYQNQVA
YQGYLFSFGLPLEGESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFGEIVSILGI
ATNNLSKKTKNDIMNTVLYIEKIDR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=412, Percent_Identity=23.3009708737864, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 49882; Mature: 49882

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQKELEIELILAHVNHKQR
CCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHH
IESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDFFQEVMKKTGATALVTAHH
HHCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEECC
ADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFLHFQKKDFPSIFHFEDTSNQE
CCHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
NHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYDLAIAELSNNINVEDLQQLFSYS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
ESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKSQYRHPIKNGYELIKEYQQFQICKI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEE
SPQADEEEDEFVLHYQNQVAYQGYLFSFGLPLEGESIQQIPVSRETSIHIRHRKTGDVLI
CCCCCCCCCCEEEEECCCCEEEEEEEEECCCCCCCCHHHCCCCCCCEEEEEECCCCCEEE
QNGHRKKLRRLFIDLKIPMEKRNSALIIEQFGEIVSILGIATNNLSKKTKNDIMNTVLYI
ECCHHHHHHHHHHHEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EKIDR
HHCCC
>Mature Secondary Structure
MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQKELEIELILAHVNHKQR
CCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHH
IESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDFFQEVMKKTGATALVTAHH
HHCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEECC
ADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFLHFQKKDFPSIFHFEDTSNQE
CCHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
NHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYDLAIAELSNNINVEDLQQLFSYS
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
ESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKSQYRHPIKNGYELIKEYQQFQICKI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEE
SPQADEEEDEFVLHYQNQVAYQGYLFSFGLPLEGESIQQIPVSRETSIHIRHRKTGDVLI
CCCCCCCCCCEEEEECCCCEEEEEEEEECCCCCCCCHHHCCCCCCCEEEEEECCCCCEEE
QNGHRKKLRRLFIDLKIPMEKRNSALIIEQFGEIVSILGIATNNLSKKTKNDIMNTVLYI
ECCHHHHHHHHHHHEECCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EKIDR
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA