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The map label for this gene is hhaIM [H]

Identifier: 169834211

GI number: 169834211

Start: 66420

End: 67187

Strand: Direct

Name: hhaIM [H]

Synonym: SPH_0083

Alternate gene names: 169834211

Gene position: 66420-67187 (Clockwise)

Preceding gene: 169832586

Following gene: 169833924

Centisome position: 2.96

GC content: 40.62

Gene sequence:

>768_bases
ATGAAATTCTTAGACTTATTTGCTGGCATTGGAGGTTTTAGGATCGGCATGGAATCAGCCGGGCATGAATGTATAGGATT
TTGTGAAATAGACAAATTCGCTAGAGCTAGTTATAAAGCTATACACGATACGAAAGGAGAAATTGAATTACATGACATTA
CAACAGTATCAGATGACACTATTCGAGGAATCGGACATGTTGATGTTATCTGTGGAGGATTTCCGTGCCAGGCTTTCAGC
ATTGCAGGAAACAGACGAGGATTTGAAGATACAAGAGGAACTCTCTTCTTTGAAATCGCAAGGTTCGCATCTATTCTCAA
ACCCAGATATCTATTCCTTGAAAACGTCAAGGGACTCCTCAACCACGACAGAGGAAATACCTTTGAGGTCATCCTCTCAG
CGTTGGATGAACTGGGGTATGATGTGGAATGGCAAGTGCTTAACAGCAAAGATTTTGGAGTCCCCCAAAATCGGGAACGT
GTGTTCATTATCGGACATTCTAGAAAAAGAGGCACCAGAAGAGTTTTTCCTATCGGAAGAGCAAATAGAGAATTTGATTT
TGAACCAAAAATAAAGATTGTTGGTAACACTAAAAATCCGAACGGGACAAGTCAAGGGACTGGGAGCGTTGTTTACGACT
CAAACGGTTTAGTCGGTACGCTTTGCGCTAGAGATTATAAAGAGCCAAAACAAGTAGCTATACCAGTATTGACACCTGAT
AGAGTAAATAAACGACAGAATGGTAGACGTTTAAAAAAAAAACGGTGA

Upstream 100 bases:

>100_bases
TTCACCCGTGATAACAATCCATTTGGCAGACCCACAAATCCATTGGATATTAGTGATGATGATTTACCGTTTTAGCGAAA
GGGGATATTCTAGTTAAGTT

Downstream 100 bases:

>100_bases
GCCTATGTTTACGCTGACGGCACAAGACCGTCATGGGATTTTAATTAAAGAAGCAACAAAAAAAGGTTATGCAGAGGCTA
CAGTTGGTGATAGTGTAAAC

Product: hypothetical protein

Products: NA

Alternate protein names: M.HhaI; Cytosine-specific methyltransferase HhaI [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKFLDLFAGIGGFRIGMESAGHECIGFCEIDKFARASYKAIHDTKGEIELHDITTVSDDTIRGIGHVDVICGGFPCQAFS
IAGNRRGFEDTRGTLFFEIARFASILKPRYLFLENVKGLLNHDRGNTFEVILSALDELGYDVEWQVLNSKDFGVPQNRER
VFIIGHSRKRGTRRVFPIGRANREFDFEPKIKIVGNTKNPNGTSQGTGSVVYDSNGLVGTLCARDYKEPKQVAIPVLTPD
RVNKRQNGRRLKKKR

Sequences:

>Translated_255_residues
MKFLDLFAGIGGFRIGMESAGHECIGFCEIDKFARASYKAIHDTKGEIELHDITTVSDDTIRGIGHVDVICGGFPCQAFS
IAGNRRGFEDTRGTLFFEIARFASILKPRYLFLENVKGLLNHDRGNTFEVILSALDELGYDVEWQVLNSKDFGVPQNRER
VFIIGHSRKRGTRRVFPIGRANREFDFEPKIKIVGNTKNPNGTSQGTGSVVYDSNGLVGTLCARDYKEPKQVAIPVLTPD
RVNKRQNGRRLKKKR
>Mature_255_residues
MKFLDLFAGIGGFRIGMESAGHECIGFCEIDKFARASYKAIHDTKGEIELHDITTVSDDTIRGIGHVDVICGGFPCQAFS
IAGNRRGFEDTRGTLFFEIARFASILKPRYLFLENVKGLLNHDRGNTFEVILSALDELGYDVEWQVLNSKDFGVPQNRER
VFIIGHSRKRGTRRVFPIGRANREFDFEPKIKIVGNTKNPNGTSQGTGSVVYDSNGLVGTLCARDYKEPKQVAIPVLTPD
RVNKRQNGRRLKKKR

Specific function: This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=290, Percent_Identity=33.7931034482759, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 28612; Mature: 28612

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLDLFAGIGGFRIGMESAGHECIGFCEIDKFARASYKAIHDTKGEIELHDITTVSDDT
CCHHHHHHCCCCEEECCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCH
IRGIGHVDVICGGFPCQAFSIAGNRRGFEDTRGTLFFEIARFASILKPRYLFLENVKGLL
HCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
NHDRGNTFEVILSALDELGYDVEWQVLNSKDFGVPQNRERVFIIGHSRKRGTRRVFPIGR
CCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCC
ANREFDFEPKIKIVGNTKNPNGTSQGTGSVVYDSNGLVGTLCARDYKEPKQVAIPVLTPD
CCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCEEEEHHHCCCCCCCEEEEEECCH
RVNKRQNGRRLKKKR
HHCCCCCCCHHCCCC
>Mature Secondary Structure
MKFLDLFAGIGGFRIGMESAGHECIGFCEIDKFARASYKAIHDTKGEIELHDITTVSDDT
CCHHHHHHCCCCEEECCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCH
IRGIGHVDVICGGFPCQAFSIAGNRRGFEDTRGTLFFEIARFASILKPRYLFLENVKGLL
HCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
NHDRGNTFEVILSALDELGYDVEWQVLNSKDFGVPQNRERVFIIGHSRKRGTRRVFPIGR
CCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEECCC
ANREFDFEPKIKIVGNTKNPNGTSQGTGSVVYDSNGLVGTLCARDYKEPKQVAIPVLTPD
CCCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCEEEEHHHCCCCCCCEEEEEECCH
RVNKRQNGRRLKKKR
HHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3549710; 8506140; 7899082; 8343957; 8293469; 9207024; 9783745; 10080885 [H]