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The map label for this gene is metE [H]

Identifier: 169833651

GI number: 169833651

Start: 628633

End: 630882

Strand: Direct

Name: metE [H]

Synonym: SPH_0684

Alternate gene names: 169833651

Gene position: 628633-630882 (Clockwise)

Preceding gene: 169834251

Following gene: 169834245

Centisome position: 27.99

GC content: 43.2

Gene sequence:

>2250_bases
ATGTCAACTACAATCGTCGGTTTCCCTCGTTTGGGCGAATTCCGTGAATTAAAATTTACAACTGAAAAATACTTTAGAAA
AGAAATCTCAGAAGAAGAACTTTTAGCAGCAGCAAAAGACTTGCGTGCTAAACACTGGAACATTGTCAAAGAAAAAGGCA
TCACTGAAATTCCATCAAATGACTTTTCTCACTATGATAACTTCCTAGATGCGGCTTTCCTTTTCAACGTGGTGCCTGCA
TCCGTTCAAAACTTGGACTTGTCTGACCTTGAGCGCTACTTCGCTTTGGGACGTGGTTACCAGGGAGAAAAAGGGGACGT
TCGCGCCCTCCCAATGAAGAAATGGTTTAACACTAACTACCACTACATCGTTCCTAAATTTGAAAAAGACACTCAAGTAA
AATTGGCTGGTCACAAGATTTTTGATGAGTTCCAAGAAGCCAAAGAACTTGGATTGAACACTCGTCCTGTTCTTGTAGGT
CCCTTCACTTTCCTTCAATTGTCAGACTTTGAAGAAGGCGTGAAAGCAGACGACTTCGTAGATAGCTTAGTGGCTGCTTA
CCAAGAAGTTTTTGCTAAATTGGCTGAACTTGGTGCGACTTGCATCCAATTGGATGAAGCGGCTCTTGTAAAAGACTTGA
CAGCTGAAGAAAAAGCTCTCTTCTTGAACCTTTACAACAAACTCTTGGCTGACAAAAAAGGTCTTGAAGTCTTGCTTCAA
ACTTATTTCGGTGATGTTCGTGACGTATACGCTGACCTTGTAAACTTGCCAGTAGATGCTATCGGTTTTGACTTTGTTGA
AGGTAAGAAAACTCTTGAACTCGTTAAAGGTGGCTTCCCAGCTGACAAGACTCTCTATGCAGGTATTGTCAATGGTAAAA
ACATCTGGCGCAACAACTATGAAAAGAGCTTGGCTGTTCTTGAGCAAATCCCAGCTGAAAACATCGTTTTGACAAGCTCT
TGCTCACTTCTTCATGTGCCATTTACAACTGCTAATGAAGAATTTGAACCAGCACTCTTGAACCACTTTGCCTTTGCAGT
TGAAAAATTGGATGAAATCCGTGACTTGGATGCTATCCGCAATGGTCAAGGTTCAGAAGCTCTTGCAGCCAACAAAGAAC
TCTTTGCGACTGAGCGTGTTGGTGAAAATGCTGAACTTCGTGCGCGTATCGCTGGCTTGACAGACACAGACTACACTCGT
TTGCCAGCCTTTGCAGAACGTGAAGCTATCCAAGAAGAAGCTTTCAAACTTCCAGCTCTTCCAACAACAACGATTGGTTC
ATTCCCTCAAACAAAAGAAGTTCGTGCCAAACGTTTGGCTTACCGTAAAGGTGAATTGTCTCAAAAAGAGTACGATGCTT
TCCTTGCTGAAACGATTGACGAATGGATCAAGTGGCAAGAAGATATTGACTTTGATGTCCTTGTTCACGGTGAATTTGAG
CGTAATGACATGGTTGAGTACTTCGGTCAAAACTTGTCAGGATACCTCTTCTCTAAAAATGGATGGGTACAATCATACGG
TATGCGTGGGGTAAAACCACCAATCATCTGGGGTGATGTCACTCGTCTTAACCCTATCACTGTTAAATGGTCTAGCTATG
CACAAAGTCGTACAAACAAACCTGTTAAAGGTATGTTGACTGGACCTGTTACCATCCTTAACTGGTCATTCCCACGTGAA
GACATCTCTATCAAGGATTCAACTCTCCAAATCGCCCTTGCTATCAAGGATGAAGTACTTGACCTTGAAGCTGCTGGTGT
GAAAATCATCCAAATCGACGAGGCTGCTCTTCGTGAAAAATTGCCACTCCGCCGTAGCGACTGGTACGAAGACTACCTTG
ACTGGGCTATTCCTGCCTTCCGCTTGGTACACTCAACAGTAGCGCCAGACACACAAATCCACACTCACATGTGTTACTCA
GAATTTACAGATATCATCCCAGCTATCGATAACATGGATGCAGACGTTATTTCCTTTGAAGCTAGCCGTTCAAACCTTGA
AATCTTGGACGAACTCAAAGCGAAAAACTTCCAAACAGAAGTGGGACCTGGGGTTTACGATATCCACTCACCTCGTGTAC
CAAATGAAGGCGAAATCGACAACACAATCGAAGCCATCCTTGCTAAAGTGCCAAGCAAGAAAGTTTGGATCAACCCTGAC
TGTGGTTTGAAAACACGTGGTATCCCAGAAACAAAAGAAAGCTTGATCCGCCTTGTTGAAGCAGCTAAAGCTGCGCGTGA
GAAATTGTAA

Upstream 100 bases:

>100_bases
ATATAGTTTCAAACTATATCAAACCCTAATTTTTTACAATTATACAGACGCTTTCTTTTCTGTTAAGATAGTTTCAACAA
CAAATTTTGGAGGACACATC

Downstream 100 bases:

>100_bases
GATTGGATTTCTCTCCATACAGTGGTTGTTCAAAAAGAAATCAACTAGAAAAGGTTATTACATATGTCACGCCAAACACC
GTCACTCTCATTTGAAGTGT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 749; Mature: 748

Protein sequence:

>749_residues
MSTTIVGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAKHWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPA
SVQNLDLSDLERYFALGRGYQGEKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATCIQLDEAALVKDLTAEEKALFLNLYNKLLADKKGLEVLLQ
TYFGDVRDVYADLVNLPVDAIGFDFVEGKKTLELVKGGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSS
CSLLHVPFTTANEEFEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAGLTDTDYTR
LPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKEYDAFLAETIDEWIKWQEDIDFDVLVHGEFE
RNDMVEYFGQNLSGYLFSKNGWVQSYGMRGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPRE
DISIKDSTLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVHSTVAPDTQIHTHMCYS
EFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVGPGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPD
CGLKTRGIPETKESLIRLVEAAKAAREKL

Sequences:

>Translated_749_residues
MSTTIVGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAKHWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPA
SVQNLDLSDLERYFALGRGYQGEKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATCIQLDEAALVKDLTAEEKALFLNLYNKLLADKKGLEVLLQ
TYFGDVRDVYADLVNLPVDAIGFDFVEGKKTLELVKGGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSS
CSLLHVPFTTANEEFEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAGLTDTDYTR
LPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKEYDAFLAETIDEWIKWQEDIDFDVLVHGEFE
RNDMVEYFGQNLSGYLFSKNGWVQSYGMRGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPRE
DISIKDSTLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVHSTVAPDTQIHTHMCYS
EFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVGPGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPD
CGLKTRGIPETKESLIRLVEAAKAAREKL
>Mature_748_residues
STTIVGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAKHWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPAS
VQNLDLSDLERYFALGRGYQGEKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVGP
FTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATCIQLDEAALVKDLTAEEKALFLNLYNKLLADKKGLEVLLQT
YFGDVRDVYADLVNLPVDAIGFDFVEGKKTLELVKGGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSC
SLLHVPFTTANEEFEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAGLTDTDYTRL
PAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKEYDAFLAETIDEWIKWQEDIDFDVLVHGEFER
NDMVEYFGQNLSGYLFSKNGWVQSYGMRGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPRED
ISIKDSTLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVHSTVAPDTQIHTHMCYSE
FTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVGPGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDC
GLKTRGIPETKESLIRLVEAAKAAREKL

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=752, Percent_Identity=46.0106382978723, Blast_Score=646, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=776, Percent_Identity=46.0051546391753, Blast_Score=650, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 84618; Mature: 84487

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTIVGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAKHWNIVKEKGITEIPSN
CCCCEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQGEKGDVRALPMKKWFNTNY
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEECHHHHHCCCC
HYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVGPFTFLQLSDFEEGVKADDFV
EEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEECCHHCCCCHHHHH
DSLVAAYQEVFAKLAELGATCIQLDEAALVKDLTAEEKALFLNLYNKLLADKKGLEVLLQ
HHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TYFGDVRDVYADLVNLPVDAIGFDFVEGKKTLELVKGGFPADKTLYAGIVNGKNIWRNNY
HHHHHHHHHHHHHHHCCHHHHCCHHHCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHCCH
EKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEEFEPALLNHFAFAVEKLDEIRDLDAIR
HHHHHHHHHCCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NGQGSEALAANKELFATERVGENAELRARIAGLTDTDYTRLPAFAEREAIQEEAFKLPAL
CCCCCCHHHCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCC
PTTTIGSFPQTKEVRAKRLAYRKGELSQKEYDAFLAETIDEWIKWQEDIDFDVLVHGEFE
CCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
RNDMVEYFGQNLSGYLFSKNGWVQSYGMRGVKPPIIWGDVTRLNPITVKWSSYAQSRTNK
CHHHHHHHCCCCCEEEEECCCCHHHCCCCCCCCCEEECCCCCCCCEEEEECHHHHHHCCC
PVKGMLTGPVTILNWSFPREDISIKDSTLQIALAIKDEVLDLEAAGVKIIQIDEAALREK
CCCEEEECCEEEEECCCCCCCCCCCCCEEEEEEEEHHHHHHHHHCCEEEEEECHHHHHHC
LPLRRSDWYEDYLDWAIPAFRLVHSTVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ASRSNLEILDELKAKNFQTEVGPGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPD
CCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCC
CGLKTRGIPETKESLIRLVEAAKAAREKL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STTIVGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAKHWNIVKEKGITEIPSN
CCCEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQGEKGDVRALPMKKWFNTNY
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEECHHHHHCCCC
HYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVGPFTFLQLSDFEEGVKADDFV
EEEEECCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEECCHHCCCCHHHHH
DSLVAAYQEVFAKLAELGATCIQLDEAALVKDLTAEEKALFLNLYNKLLADKKGLEVLLQ
HHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TYFGDVRDVYADLVNLPVDAIGFDFVEGKKTLELVKGGFPADKTLYAGIVNGKNIWRNNY
HHHHHHHHHHHHHHHCCHHHHCCHHHCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHCCH
EKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEEFEPALLNHFAFAVEKLDEIRDLDAIR
HHHHHHHHHCCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NGQGSEALAANKELFATERVGENAELRARIAGLTDTDYTRLPAFAEREAIQEEAFKLPAL
CCCCCCHHHCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCC
PTTTIGSFPQTKEVRAKRLAYRKGELSQKEYDAFLAETIDEWIKWQEDIDFDVLVHGEFE
CCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
RNDMVEYFGQNLSGYLFSKNGWVQSYGMRGVKPPIIWGDVTRLNPITVKWSSYAQSRTNK
CHHHHHHHCCCCCEEEEECCCCHHHCCCCCCCCCEEECCCCCCCCEEEEECHHHHHHCCC
PVKGMLTGPVTILNWSFPREDISIKDSTLQIALAIKDEVLDLEAAGVKIIQIDEAALREK
CCCEEEECCEEEEECCCCCCCCCCCCCEEEEEEEEHHHHHHHHHCCEEEEEECHHHHHHC
LPLRRSDWYEDYLDWAIPAFRLVHSTVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFE
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
ASRSNLEILDELKAKNFQTEVGPGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPD
CCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCC
CGLKTRGIPETKESLIRLVEAAKAAREKL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA